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The map label for this gene is hyuB-2

Identifier: 15898802

GI number: 15898802

Start: 1825283

End: 1826815

Strand: Direct

Name: hyuB-2

Synonym: SSO2010

Alternate gene names: NA

Gene position: 1825283-1826815 (Clockwise)

Preceding gene: 15898801

Following gene: 15898803

Centisome position: 61.0

GC content: 38.75

Gene sequence:

>1533_bases
ATGACGAGTTGGGAAATAATTCATAAGGCTTTTGAATTTATTGCAGAAGAAATGGGAGTTATGTTAAAGAGATCGGCGAT
GTCACCTAATATTAGGGAGAGAATGGACCACAGTTGTGCCATTTTAGACGCTGAGGGAAATATCATAGCTCAGGCTGAGC
ATATACCCGTTCATCTAGGTTCCTTCAGTGTAGGAGTTAAGAATACTCTGAAAATCGTTAATTTAGAAGAGGGCGATATG
GTTATATTAAATGACCCTTACATTTCTGGTACTCACTTAAATGACGTAATGCTGTTAGCCCCTATTTATTATAACGGTAA
ACTCATAGGATACGTTGTTAATAAAGCTCATCATGTAGACGTAGGAGGACCTTCTCCAGGGAGTTTAAACCCTTATGCCA
CTACAATATATGAGGAGGGACTTGTAATACCTCCAGTAAAGATAATTAAGAAGGGTAAGCTAAACGAGGAGATAGTTGAG
ATTATTAGAGAGAACTTTAAGGTTCCAGAAGTATCCTTAGGTGATTTAAATGCTCAGATCTCAGCTAACTTAAACGGTGT
ATTGAGAGTAAAGCAGCTAATAGATAAATACGGCTTAGATAGAGTTTTATCTTCCTGGGCAAAATCAATTGAATACGGAA
GATCCTTAGCTCTTTCTGAAATTTCTAAATGGAATGAGGGTTCATTTGAGGATGAGGATTACTTAGAATTGGATACTTTA
AAGAGAATAAGGGTTAAAATAACTATTAGTGATAAGGGAATAATTGCAGACTTTAATGGAACTGATAATCAAGTTGAAGC
TCCATTTAACGCAGTATACGGTGTTACTTTTTCTGCAGTGAGTTTTGTGGTTAGGTCTTTAATAGGGAAGGACATTCCTA
CTAATGAGGGGTTTTATAGCGTAATTAAGGTAATAGCACCAGAGGGTACTATAGTTAATCCATTAAAGCCTGCAGCAGTC
GGAGGAGGAAACGTTGAAACTTCACAGAGGATTGCAGATGTCACATTTAAAGCGTTTTCACACTCAATTCCAGTACCAGC
TGCAAGCTCTGGTACCATGATGAACGTAATGATGGGGGGCACTTATAAGGGAAAATATTGGGCTTACTATGAGACTATAG
GGGGAGGTATGGGGGGAAGACCAAATAAGGATGGAGTGTCTGGGGTTCATGTAAATATGACGAATACGTTAAATACTCCC
ATAGAAATAGCTGAAAGGCATTATCCAATACTTTTCACTACATATAGAATAAGAGAAGGAAGCGGAGGAAAGGGTATGTA
TAGGGGAGGAGATGGAATAATAAGAGCATTTAAGGTTTTAGAGAAAACTAAGCTCTCAATAATGGCTGAAAGATTTAAGG
TTCCACCTTGGGGATTAAAGGGAGGAGAGAACGGAAAGCCAGGGAGGGTTACAATTATCAGAAAGGGTAATGTTGTAGAG
GAAATGCCCAGTAAGTTCTCCACTATTTTAAATCCGGGAGATGAGGTAATAATTGAAACCCCAGGGGGAGGAGGATATGG
AAGCGAGAAATAG

Upstream 100 bases:

>100_bases
AAATAAGGGGTCCGGCAATTATTGAGGAGTATAGCTCTACTATCGTGGTCAAGCCAGGTTGGAAGGCTTTAGTAGATGAT
TCAATTATAATGGTGAGAGA

Downstream 100 bases:

>100_bases
TGATCTATTACTCTTTTTTCATTCAATTTTAGCTAAACATTTTAAATTATAAGAGGCATGTAAGTAACAGTTTATGGCTA
TAAAATTAGAAAAGTTAATT

Product: N-methyl hydantoinase B (hyuB-2)

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MTSWEIIHKAFEFIAEEMGVMLKRSAMSPNIRERMDHSCAILDAEGNIIAQAEHIPVHLGSFSVGVKNTLKIVNLEEGDM
VILNDPYISGTHLNDVMLLAPIYYNGKLIGYVVNKAHHVDVGGPSPGSLNPYATTIYEEGLVIPPVKIIKKGKLNEEIVE
IIRENFKVPEVSLGDLNAQISANLNGVLRVKQLIDKYGLDRVLSSWAKSIEYGRSLALSEISKWNEGSFEDEDYLELDTL
KRIRVKITISDKGIIADFNGTDNQVEAPFNAVYGVTFSAVSFVVRSLIGKDIPTNEGFYSVIKVIAPEGTIVNPLKPAAV
GGGNVETSQRIADVTFKAFSHSIPVPAASSGTMMNVMMGGTYKGKYWAYYETIGGGMGGRPNKDGVSGVHVNMTNTLNTP
IEIAERHYPILFTTYRIREGSGGKGMYRGGDGIIRAFKVLEKTKLSIMAERFKVPPWGLKGGENGKPGRVTIIRKGNVVE
EMPSKFSTILNPGDEVIIETPGGGGYGSEK

Sequences:

>Translated_510_residues
MTSWEIIHKAFEFIAEEMGVMLKRSAMSPNIRERMDHSCAILDAEGNIIAQAEHIPVHLGSFSVGVKNTLKIVNLEEGDM
VILNDPYISGTHLNDVMLLAPIYYNGKLIGYVVNKAHHVDVGGPSPGSLNPYATTIYEEGLVIPPVKIIKKGKLNEEIVE
IIRENFKVPEVSLGDLNAQISANLNGVLRVKQLIDKYGLDRVLSSWAKSIEYGRSLALSEISKWNEGSFEDEDYLELDTL
KRIRVKITISDKGIIADFNGTDNQVEAPFNAVYGVTFSAVSFVVRSLIGKDIPTNEGFYSVIKVIAPEGTIVNPLKPAAV
GGGNVETSQRIADVTFKAFSHSIPVPAASSGTMMNVMMGGTYKGKYWAYYETIGGGMGGRPNKDGVSGVHVNMTNTLNTP
IEIAERHYPILFTTYRIREGSGGKGMYRGGDGIIRAFKVLEKTKLSIMAERFKVPPWGLKGGENGKPGRVTIIRKGNVVE
EMPSKFSTILNPGDEVIIETPGGGGYGSEK
>Mature_509_residues
TSWEIIHKAFEFIAEEMGVMLKRSAMSPNIRERMDHSCAILDAEGNIIAQAEHIPVHLGSFSVGVKNTLKIVNLEEGDMV
ILNDPYISGTHLNDVMLLAPIYYNGKLIGYVVNKAHHVDVGGPSPGSLNPYATTIYEEGLVIPPVKIIKKGKLNEEIVEI
IRENFKVPEVSLGDLNAQISANLNGVLRVKQLIDKYGLDRVLSSWAKSIEYGRSLALSEISKWNEGSFEDEDYLELDTLK
RIRVKITISDKGIIADFNGTDNQVEAPFNAVYGVTFSAVSFVVRSLIGKDIPTNEGFYSVIKVIAPEGTIVNPLKPAAVG
GGNVETSQRIADVTFKAFSHSIPVPAASSGTMMNVMMGGTYKGKYWAYYETIGGGMGGRPNKDGVSGVHVNMTNTLNTPI
EIAERHYPILFTTYRIREGSGGKGMYRGGDGIIRAFKVLEKTKLSIMAERFKVPPWGLKGGENGKPGRVTIIRKGNVVEE
MPSKFSTILNPGDEVIIETPGGGGYGSEK

Specific function: Unknown

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=524, Percent_Identity=39.3129770992366, Blast_Score=338, Evalue=8e-93,
Organism=Caenorhabditis elegans, GI133901900, Length=526, Percent_Identity=38.4030418250951, Blast_Score=343, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI133901902, Length=502, Percent_Identity=37.6494023904382, Blast_Score=319, Evalue=3e-87,
Organism=Saccharomyces cerevisiae, GI6322634, Length=537, Percent_Identity=36.6852886405959, Blast_Score=325, Evalue=7e-90,
Organism=Drosophila melanogaster, GI45550492, Length=528, Percent_Identity=35.7954545454545, Blast_Score=320, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.14

Molecular weight: Translated: 55615; Mature: 55483

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSWEIIHKAFEFIAEEMGVMLKRSAMSPNIRERMDHSCAILDAEGNIIAQAEHIPVHLG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCEEEEECCCEEEEC
SFSVGVKNTLKIVNLEEGDMVILNDPYISGTHLNDVMLLAPIYYNGKLIGYVVNKAHHVD
CEEECCCCEEEEEEECCCCEEEEECCEECCCCCCCEEEEEEEEECCEEEEEEECCEEEEE
VGGPSPGSLNPYATTIYEEGLVIPPVKIIKKGKLNEEIVEIIRENFKVPEVSLGDLNAQI
CCCCCCCCCCCCEEEEECCCEECCCHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCCEEE
SANLNGVLRVKQLIDKYGLDRVLSSWAKSIEYGRSLALSEISKWNEGSFEDEDYLELDTL
ECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCEEHHCE
KRIRVKITISDKGIIADFNGTDNQVEAPFNAVYGVTFSAVSFVVRSLIGKDIPTNEGFYS
EEEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
VIKVIAPEGTIVNPLKPAAVGGGNVETSQRIADVTFKAFSHSIPVPAASSGTMMNVMMGG
HHHHCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECC
TYKGKYWAYYETIGGGMGGRPNKDGVSGVHVNMTNTLNTPIEIAERHYPILFTTYRIREG
CCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCEEEEEEEEEEC
SGGKGMYRGGDGIIRAFKVLEKTKLSIMAERFKVPPWGLKGGENGKPGRVTIIRKGNVVE
CCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHH
EMPSKFSTILNPGDEVIIETPGGGGYGSEK
HHHHHHHHHCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TSWEIIHKAFEFIAEEMGVMLKRSAMSPNIRERMDHSCAILDAEGNIIAQAEHIPVHLG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCEEEEECCCEEEEC
SFSVGVKNTLKIVNLEEGDMVILNDPYISGTHLNDVMLLAPIYYNGKLIGYVVNKAHHVD
CEEECCCCEEEEEEECCCCEEEEECCEECCCCCCCEEEEEEEEECCEEEEEEECCEEEEE
VGGPSPGSLNPYATTIYEEGLVIPPVKIIKKGKLNEEIVEIIRENFKVPEVSLGDLNAQI
CCCCCCCCCCCCEEEEECCCEECCCHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCCEEE
SANLNGVLRVKQLIDKYGLDRVLSSWAKSIEYGRSLALSEISKWNEGSFEDEDYLELDTL
ECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCEEHHCE
KRIRVKITISDKGIIADFNGTDNQVEAPFNAVYGVTFSAVSFVVRSLIGKDIPTNEGFYS
EEEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
VIKVIAPEGTIVNPLKPAAVGGGNVETSQRIADVTFKAFSHSIPVPAASSGTMMNVMMGG
HHHHCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECC
TYKGKYWAYYETIGGGMGGRPNKDGVSGVHVNMTNTLNTPIEIAERHYPILFTTYRIREG
CCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCEEEEEEEEEEC
SGGKGMYRGGDGIIRAFKVLEKTKLSIMAERFKVPPWGLKGGENGKPGRVTIIRKGNVVE
CCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHH
EMPSKFSTILNPGDEVIIETPGGGGYGSEK
HHHHHHHHHCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]