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The map label for this gene is thiC-2 [H]

Identifier: 15898740

GI number: 15898740

Start: 1759304

End: 1760605

Strand: Direct

Name: thiC-2 [H]

Synonym: SSO1941

Alternate gene names: 15898740

Gene position: 1759304-1760605 (Clockwise)

Preceding gene: 15898739

Following gene: 15898741

Centisome position: 58.8

GC content: 42.17

Gene sequence:

>1302_bases
ATGGGCATCATAGATGAGGCAAAAAGGGGTCAGATAACAGATGAGATGAGGGCAATAAGTAAGCTAGAAGGTATACCAGT
AGAGAAGGTCAGAAATAGGATAAGTGAAGGTAAAATAATGTTGATAAGAAATGCGAAGTACCCCAGTAGAAAACTTGTCC
CAATAGGTAAGGGACTAACTACTAAAGTTAACGTAAACATAGGCACTTCAAGTGAGGTTGTAGACTTAGACATGGAATTA
CAGAAGGTAAAGGTTGCAAACAAGTGGGGAGATACTCTAATGGATTTATCAACGGGAGGAGACTTAGATGCCATAAGGAG
GGATATAATAAAGGCATCAGATCTACCAGTTGGTACAGTCCCAGTTTACCAAATTTTCATAGAGTCCTTTAAGAAGAAGT
CTGGAGGAGCGTATTTTACTGAAGATGAATTACTAAACACAGTGGAAAAGCACTTAAAGGATGGGGTTGCATTCATGACA
ATTCACGCTGGAATAACTAAGGATTTAGCTATTAGGGCGTTAAAGAGCGATAGGATTATTCCAATAGTCTCAAGGGGAGG
GGACATGATAGCTGGTTGGATGATACACAATAACTCGGAGAACCCCTATAGAAAGAACTGGGATTACGTGTTAGAAATGT
TTAAGGAATATGATGCTGTAATCTCTTTGGGAGATGCCTTAAGACCTGGAGCTACTGGAGACGCTCATGACGAGTTCCAA
ATTGGAGAACTATTGGAAACCGCTAGGCTAGTTAAAAGTGCGTTGCAAAAGGGTGTGCAAGTTATGGTTGAGGGACCGGG
ACACGTACCGCTGAACGAAATAGCTTGGGACGTTAAGTTAATGAAGAAGTTAACTGGTGGTGTACCATATTACGTTTTGG
GTCCCTTGCCCATTGATGTAGGTGCACCCTATGATCACATAGCCTCTGCAATAGGTGCGGCAATATCATCAGCTAGTGGT
GTTGACTTATTATGTTATCTAACCCCAGCTGAGCACTTAGGGTTACCAACTGTTAAGCAAGTTGAGGAGGGGGCAATCGC
TTATAGGATTGCTGCCCATGCAGGTGATGTGGTTAAGTTAGGCAGGAAAGCTAGGAAGTGGGATGACGAGGTCAGTTATT
ATAGGGGGAAACTGGATTGGGAGAACATGATTTCTAAGCTAATAGATCCGCAAAGGGCTTATCAAGTTTACACTCAGTTT
GGTACTCCTAAAGTGAAAGCTTGTACCATGTGTGGTGGATATTGTCCAATGATGTGGGCTATGGATCAAGTTAGGAAGAT
AGGTTCTTCATCATCCCTATAA

Upstream 100 bases:

>100_bases
GATGAACCAAATAGAAAAGGCTTAATTTTAGCAGCTAAAGACTCTCCGGCATCTTATAGTATATAATAATCTTTATAGCT
TAGTTATATGAATATTTCTT

Downstream 100 bases:

>100_bases
GTACGGATTCGTATTTTTATTTTCAAACAACAACACTTTTTTATGGATAAAACAGTTTTAGACGCGAGTTTAGAACCTTT
AAAGGGAAAGACAATAGCCG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase 1; HMP-P synthase 1; HMP-phosphate synthase 1; HMPP synthase 1; Thiamine biosynthesis protein thiC 1 [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MGIIDEAKRGQITDEMRAISKLEGIPVEKVRNRISEGKIMLIRNAKYPSRKLVPIGKGLTTKVNVNIGTSSEVVDLDMEL
QKVKVANKWGDTLMDLSTGGDLDAIRRDIIKASDLPVGTVPVYQIFIESFKKKSGGAYFTEDELLNTVEKHLKDGVAFMT
IHAGITKDLAIRALKSDRIIPIVSRGGDMIAGWMIHNNSENPYRKNWDYVLEMFKEYDAVISLGDALRPGATGDAHDEFQ
IGELLETARLVKSALQKGVQVMVEGPGHVPLNEIAWDVKLMKKLTGGVPYYVLGPLPIDVGAPYDHIASAIGAAISSASG
VDLLCYLTPAEHLGLPTVKQVEEGAIAYRIAAHAGDVVKLGRKARKWDDEVSYYRGKLDWENMISKLIDPQRAYQVYTQF
GTPKVKACTMCGGYCPMMWAMDQVRKIGSSSSL

Sequences:

>Translated_433_residues
MGIIDEAKRGQITDEMRAISKLEGIPVEKVRNRISEGKIMLIRNAKYPSRKLVPIGKGLTTKVNVNIGTSSEVVDLDMEL
QKVKVANKWGDTLMDLSTGGDLDAIRRDIIKASDLPVGTVPVYQIFIESFKKKSGGAYFTEDELLNTVEKHLKDGVAFMT
IHAGITKDLAIRALKSDRIIPIVSRGGDMIAGWMIHNNSENPYRKNWDYVLEMFKEYDAVISLGDALRPGATGDAHDEFQ
IGELLETARLVKSALQKGVQVMVEGPGHVPLNEIAWDVKLMKKLTGGVPYYVLGPLPIDVGAPYDHIASAIGAAISSASG
VDLLCYLTPAEHLGLPTVKQVEEGAIAYRIAAHAGDVVKLGRKARKWDDEVSYYRGKLDWENMISKLIDPQRAYQVYTQF
GTPKVKACTMCGGYCPMMWAMDQVRKIGSSSSL
>Mature_432_residues
GIIDEAKRGQITDEMRAISKLEGIPVEKVRNRISEGKIMLIRNAKYPSRKLVPIGKGLTTKVNVNIGTSSEVVDLDMELQ
KVKVANKWGDTLMDLSTGGDLDAIRRDIIKASDLPVGTVPVYQIFIESFKKKSGGAYFTEDELLNTVEKHLKDGVAFMTI
HAGITKDLAIRALKSDRIIPIVSRGGDMIAGWMIHNNSENPYRKNWDYVLEMFKEYDAVISLGDALRPGATGDAHDEFQI
GELLETARLVKSALQKGVQVMVEGPGHVPLNEIAWDVKLMKKLTGGVPYYVLGPLPIDVGAPYDHIASAIGAAISSASGV
DLLCYLTPAEHLGLPTVKQVEEGAIAYRIAAHAGDVVKLGRKARKWDDEVSYYRGKLDWENMISKLIDPQRAYQVYTQFG
TPKVKACTMCGGYCPMMWAMDQVRKIGSSSSL

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=418, Percent_Identity=37.0813397129187, Blast_Score=269, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 47642; Mature: 47511

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIIDEAKRGQITDEMRAISKLEGIPVEKVRNRISEGKIMLIRNAKYPSRKLVPIGKGLT
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCE
TKVNVNIGTSSEVVDLDMELQKVKVANKWGDTLMDLSTGGDLDAIRRDIIKASDLPVGTV
EEEEEEECCCCCEEEECHHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHCCCCCCCH
PVYQIFIESFKKKSGGAYFTEDELLNTVEKHLKDGVAFMTIHAGITKDLAIRALKSDRII
HHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCCEE
PIVSRGGDMIAGWMIHNNSENPYRKNWDYVLEMFKEYDAVISLGDALRPGATGDAHDEFQ
EEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
IGELLETARLVKSALQKGVQVMVEGPGHVPLNEIAWDVKLMKKLTGGVPYYVLGPLPIDV
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECCEEECC
GAPYDHIASAIGAAISSASGVDLLCYLTPAEHLGLPTVKQVEEGAIAYRIAAHAGDVVKL
CCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHCCCCCHHHHHCCCEEEEEECCCCHHHHH
GRKARKWDDEVSYYRGKLDWENMISKLIDPQRAYQVYTQFGTPKVKACTMCGGYCPMMWA
HHHHHCCCHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEHHCCCCHHHHHH
MDQVRKIGSSSSL
HHHHHHCCCCCCC
>Mature Secondary Structure 
GIIDEAKRGQITDEMRAISKLEGIPVEKVRNRISEGKIMLIRNAKYPSRKLVPIGKGLT
CCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCE
TKVNVNIGTSSEVVDLDMELQKVKVANKWGDTLMDLSTGGDLDAIRRDIIKASDLPVGTV
EEEEEEECCCCCEEEECHHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHCCCCCCCH
PVYQIFIESFKKKSGGAYFTEDELLNTVEKHLKDGVAFMTIHAGITKDLAIRALKSDRII
HHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCCEE
PIVSRGGDMIAGWMIHNNSENPYRKNWDYVLEMFKEYDAVISLGDALRPGATGDAHDEFQ
EEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
IGELLETARLVKSALQKGVQVMVEGPGHVPLNEIAWDVKLMKKLTGGVPYYVLGPLPIDV
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECCEEECC
GAPYDHIASAIGAAISSASGVDLLCYLTPAEHLGLPTVKQVEEGAIAYRIAAHAGDVVKL
CCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHCCCCCHHHHHCCCEEEEEECCCCHHHHH
GRKARKWDDEVSYYRGKLDWENMISKLIDPQRAYQVYTQFGTPKVKACTMCGGYCPMMWA
HHHHHCCCHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEHHCCCCHHHHHH
MDQVRKIGSSSSL
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]