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The map label for this gene is rfbB-2 [H]

Identifier: 15898538

GI number: 15898538

Start: 1573177

End: 1574229

Strand: Direct

Name: rfbB-2 [H]

Synonym: SSO1734

Alternate gene names: 15898538

Gene position: 1573177-1574229 (Clockwise)

Preceding gene: 15898536

Following gene: 15898539

Centisome position: 52.58

GC content: 39.03

Gene sequence:

>1053_bases
TTGAACCGCGTTCAACTTTCTCAACGCTCTTCTCCCATCTTTCCCCAACCTGCTCAAGTAGCTCCCACTCGCCTTAAGCA
AGGCACTGAGGAGTTCCTCAGGGAAAAGTATTTTAGTGAGGACCGAGTAAATTATCTTGAGGCGGACCTGGCGAAGTCCC
GGTTGAACTTCGCCGGTTTAGTTGTATTCTCATGCTTCATATGTTCAAGTCCGCCCTCGTATATACAAACTTTTCTACAG
AAATTCTCATCCTCTCAACTTTTTCTGTATTTCATTCATCTCGTTTTTTTACGAGAAAAAAGAGATTCCCATACTGACGT
GGATAGGTCAATTTACAAACCTCAAGATTTTGTCTCAACGAACGTCTTTGGGACTGTAAACGTATTAGAGGCTGCAAGAA
GGTATAACTTTAAGTATGTTCATATCTCCACTGATGAAGTATACGGAGAGGAGTGCTCAGACGAGAACTCATCCGTAAAC
CCATCATCTCCTTATAGCGCATCTAAAGCGTCAGCTGATCTATTCGTAAAATCCTACATTAAGACGTATAATGTTGAGGC
TATAATAGTTAGACCTTCCAATAACTATGGACCTAGACAATTTCCAGAGAAATTTATTCCAAAGGCTATAATTCGTACAA
TTCTAGGAATTTATGTACCAATTTATGGTAATGGGGAACAAGAGAGGGATTGGATTTACGTAGAGGATACTGCTAGGGTA
ATTTTCGATTTGATTCGAAGTGCAGAGTGGAGAGGTGAAGTGTATAATATTCCGGGAGGGTATAGGGTTAAGAACATTCA
ATTAATAAGACTGTTGGAGAGGGTAATCAAAAAAGAGATTAAAGTTAAGTACGTTTCGGATAGACCTGGACATGATAGAA
GATATTGCATGATAAATACTAAACTAAATTACACTACAACTCCCCTAGAGGAGGGATTAAGGAAAACGTATGATTGGTAC
GCTAATAATAATTGGTGGTGGAGTTCGCTAGTTAATGATGAATTTTTCAAATCTGATGAACCCTGGAGACCTTACGAAGA
TAAAAAATACTAA

Upstream 100 bases:

>100_bases
ATTGAGGTATGGCATGGAAGTCCACTGCTACCCTCCTGTCCCTGACTCCCCTTAACGTCATCCTCCCCATCTTCCTCAAC
GCATCCCTCACGTCCTCAAC

Downstream 100 bases:

>100_bases
CGCACACACTTTTGAACCCTTTTAACTTCCCCCTCTCTAACCAAAATAGCAACGGCATTTTTCTGGAGAATGTTCTCATG
ACCTTATTTAAGTTGTTTAA

Product: dTDP-glucose 4,6-dehydratase (rfbB-2)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MNRVQLSQRSSPIFPQPAQVAPTRLKQGTEEFLREKYFSEDRVNYLEADLAKSRLNFAGLVVFSCFICSSPPSYIQTFLQ
KFSSSQLFLYFIHLVFLREKRDSHTDVDRSIYKPQDFVSTNVFGTVNVLEAARRYNFKYVHISTDEVYGEECSDENSSVN
PSSPYSASKASADLFVKSYIKTYNVEAIIVRPSNNYGPRQFPEKFIPKAIIRTILGIYVPIYGNGEQERDWIYVEDTARV
IFDLIRSAEWRGEVYNIPGGYRVKNIQLIRLLERVIKKEIKVKYVSDRPGHDRRYCMINTKLNYTTTPLEEGLRKTYDWY
ANNNWWWSSLVNDEFFKSDEPWRPYEDKKY

Sequences:

>Translated_350_residues
MNRVQLSQRSSPIFPQPAQVAPTRLKQGTEEFLREKYFSEDRVNYLEADLAKSRLNFAGLVVFSCFICSSPPSYIQTFLQ
KFSSSQLFLYFIHLVFLREKRDSHTDVDRSIYKPQDFVSTNVFGTVNVLEAARRYNFKYVHISTDEVYGEECSDENSSVN
PSSPYSASKASADLFVKSYIKTYNVEAIIVRPSNNYGPRQFPEKFIPKAIIRTILGIYVPIYGNGEQERDWIYVEDTARV
IFDLIRSAEWRGEVYNIPGGYRVKNIQLIRLLERVIKKEIKVKYVSDRPGHDRRYCMINTKLNYTTTPLEEGLRKTYDWY
ANNNWWWSSLVNDEFFKSDEPWRPYEDKKY
>Mature_350_residues
MNRVQLSQRSSPIFPQPAQVAPTRLKQGTEEFLREKYFSEDRVNYLEADLAKSRLNFAGLVVFSCFICSSPPSYIQTFLQ
KFSSSQLFLYFIHLVFLREKRDSHTDVDRSIYKPQDFVSTNVFGTVNVLEAARRYNFKYVHISTDEVYGEECSDENSSVN
PSSPYSASKASADLFVKSYIKTYNVEAIIVRPSNNYGPRQFPEKFIPKAIIRTILGIYVPIYGNGEQERDWIYVEDTARV
IFDLIRSAEWRGEVYNIPGGYRVKNIQLIRLLERVIKKEIKVKYVSDRPGHDRRYCMINTKLNYTTTPLEEGLRKTYDWY
ANNNWWWSSLVNDEFFKSDEPWRPYEDKKY

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=259, Percent_Identity=36.2934362934363, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI42516563, Length=238, Percent_Identity=26.890756302521, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1788353, Length=296, Percent_Identity=39.1891891891892, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI48994969, Length=265, Percent_Identity=40, Blast_Score=188, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17568069, Length=238, Percent_Identity=35.2941176470588, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI115532424, Length=225, Percent_Identity=32.8888888888889, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17539532, Length=147, Percent_Identity=29.9319727891156, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21356223, Length=231, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 41170; Mature: 41170

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRVQLSQRSSPIFPQPAQVAPTRLKQGTEEFLREKYFSEDRVNYLEADLAKSRLNFAGL
CCCCCCCCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VVFSCFICSSPPSYIQTFLQKFSSSQLFLYFIHLVFLREKRDSHTDVDRSIYKPQDFVST
HHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHC
NVFGTVNVLEAARRYNFKYVHISTDEVYGEECSDENSSVNPSSPYSASKASADLFVKSYI
CCCHHHHHHHHHHHCCCEEEEEECHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KTYNVEAIIVRPSNNYGPRQFPEKFIPKAIIRTILGIYVPIYGNGEQERDWIYVEDTARV
HHCCCEEEEEECCCCCCCHHCCHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEHHHHH
IFDLIRSAEWRGEVYNIPGGYRVKNIQLIRLLERVIKKEIKVKYVSDRPGHDRRYCMINT
HHHHHHCCCCCCCEEECCCCCEECHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEC
KLNYTTTPLEEGLRKTYDWYANNNWWWSSLVNDEFFKSDEPWRPYEDKKY
CCCCEECHHHHHHHHHHHHHCCCCEEEHHHCCHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MNRVQLSQRSSPIFPQPAQVAPTRLKQGTEEFLREKYFSEDRVNYLEADLAKSRLNFAGL
CCCCCCCCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VVFSCFICSSPPSYIQTFLQKFSSSQLFLYFIHLVFLREKRDSHTDVDRSIYKPQDFVST
HHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHC
NVFGTVNVLEAARRYNFKYVHISTDEVYGEECSDENSSVNPSSPYSASKASADLFVKSYI
CCCHHHHHHHHHHHCCCEEEEEECHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KTYNVEAIIVRPSNNYGPRQFPEKFIPKAIIRTILGIYVPIYGNGEQERDWIYVEDTARV
HHCCCEEEEEECCCCCCCHHCCHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEHHHHH
IFDLIRSAEWRGEVYNIPGGYRVKNIQLIRLLERVIKKEIKVKYVSDRPGHDRRYCMINT
HHHHHHCCCCCCCEEECCCCCEECHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEC
KLNYTTTPLEEGLRKTYDWYANNNWWWSSLVNDEFFKSDEPWRPYEDKKY
CCCCEECHHHHHHHHHHHHHCCCCEEEHHHCCHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8022265; 10710307 [H]