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The map label for this gene is alkK-1 [H]

Identifier: 15898409

GI number: 15898409

Start: 1439908

End: 1441335

Strand: Direct

Name: alkK-1 [H]

Synonym: SSO1595

Alternate gene names: 15898409

Gene position: 1439908-1441335 (Clockwise)

Preceding gene: 15898406

Following gene: 15898412

Centisome position: 48.12

GC content: 40.2

Gene sequence:

>1428_bases
TTGATGTCTTCAGTTAAAAACGAATACGATGCAATTCATAGGGAATTACGAGAACAAGGTTTTATTAGAACTGATTATCC
CCCAAATCCATCTGAAGTGTTATGGAATAAGAAGATAATGACAATGAAAAGAGAGGAAATCGATAAGTTAAAGACGTTTA
GACTAAGAAGGATTATAAAGTGGGCATGGGAAAACATTGAGTTCTATAGAAGGTTTTGGAAATCTAAAAACTTCGAACCA
GACATGATAAAGGACTGGAGGGATATCGCTAAAATACCGATATTGAGAAAAGATGAAATTAGGAAAGACCTACAGCAAAA
TCCTCCATTTGGTACAATTTTCCATCCAGAATTGGCTAATCACATAAGATTTGTTGGAGCGACTTCTGGCTCAACTGGAT
TACCTACCTTCCAAGGATGGGGGGCTTTGGAAATGGACTACTTCCAAGAAGCCCAGGCAAGGTACTTGTGGACTTTTGCC
GAAGTTAAGCCAACTAAGGTTTACGCAAACTACCTAAACATGAGTGGCTTTTATAGTTGGGGGCCTCCGGTTGTTGAGAC
CGCAATGTGGAGATGTGGGGCAACTGTCATAGCTGGAGGTGGTGAGACATACTTCTCATGGAAGAATAGACACAACCTGA
TCTTCAAACTATGGAAAGTTGACGTATTCGCCACAACTCCTTGGCTACATAGATTAGTAGGAGAGGAGGCTAAAATTGAG
GGTTGGGAAACTCCCTTCAAGGTTTTGCTACTTCACGGTGGTGCAGCTGCTGAAAACACTAAAAAGAAATTATTCAAGGT
TCATCCAAATGCTGAATTAGCTATAAGTGTTTGGGGAACCACTGATGGACATATGGCTATTGAAGTTCCAAGCTTAGAAG
GACAATTAGTAGTTTGGGAGGACATGGAGATATTTGATATTGTAGACCCTAAGACTGATGAACCAGTATCTGGGGGAGAA
AGAGGTGAACTGATAGCGACCTTGTTAAATCACTTTACCATGCCTTTAATACGCTATAGTTTAGGAGATTACGTTAAGAA
TGACTTCCTGACCGATCCGGATCCTAAGTATGGTATAACTCACATGAGATTTGCTGAGCCAATACCAGGTAGGGTTGAGT
GGATGTTCTTCGTTAAAGGGAAACTATTATTGCCAATTTACGTTGAGGACGCTGTGAATGAAATTCCAGACACTACTGGA
ATGTTTAACATTTTCATTTACGATAATAGTATGGATAAATTGAAGATTAGAATAGAGACAAGGAGGGAACAGGTTAATTC
TACTTACGATAAGCAAGCCAGAGAGATATTAGCGAGCAGGATTGGGATTAACCCAGATGACGTTGAGATAGAGTGGGTAA
AGCCTGGAAATACAATATGGACGGGCTACAAGTTGCAAGTGTTTGTTGACCAGAGGAAGAAGAAATAA

Upstream 100 bases:

>100_bases
TTAACAAAATTAAAAATCGAACTTATAGGTATGACTACTCGAGGTAAGTTTCCCTTGAAAACGAATTTATAATTTTTTAA
ACTCTTTAAACATTAGAAGA

Downstream 100 bases:

>100_bases
TTTTTCTTTTTACTTATTACTTTATTATACTAGAAATCAAAAATAATAATAGAAAAGTTCTTATGTAAATAGCTCCGCCC
TAATATAGATGGTACGATGT

Product: medium-chain-fatty-acid--CoA ligase (alkK-1)

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MMSSVKNEYDAIHRELREQGFIRTDYPPNPSEVLWNKKIMTMKREEIDKLKTFRLRRIIKWAWENIEFYRRFWKSKNFEP
DMIKDWRDIAKIPILRKDEIRKDLQQNPPFGTIFHPELANHIRFVGATSGSTGLPTFQGWGALEMDYFQEAQARYLWTFA
EVKPTKVYANYLNMSGFYSWGPPVVETAMWRCGATVIAGGGETYFSWKNRHNLIFKLWKVDVFATTPWLHRLVGEEAKIE
GWETPFKVLLLHGGAAAENTKKKLFKVHPNAELAISVWGTTDGHMAIEVPSLEGQLVVWEDMEIFDIVDPKTDEPVSGGE
RGELIATLLNHFTMPLIRYSLGDYVKNDFLTDPDPKYGITHMRFAEPIPGRVEWMFFVKGKLLLPIYVEDAVNEIPDTTG
MFNIFIYDNSMDKLKIRIETRREQVNSTYDKQAREILASRIGINPDDVEIEWVKPGNTIWTGYKLQVFVDQRKKK

Sequences:

>Translated_475_residues
MMSSVKNEYDAIHRELREQGFIRTDYPPNPSEVLWNKKIMTMKREEIDKLKTFRLRRIIKWAWENIEFYRRFWKSKNFEP
DMIKDWRDIAKIPILRKDEIRKDLQQNPPFGTIFHPELANHIRFVGATSGSTGLPTFQGWGALEMDYFQEAQARYLWTFA
EVKPTKVYANYLNMSGFYSWGPPVVETAMWRCGATVIAGGGETYFSWKNRHNLIFKLWKVDVFATTPWLHRLVGEEAKIE
GWETPFKVLLLHGGAAAENTKKKLFKVHPNAELAISVWGTTDGHMAIEVPSLEGQLVVWEDMEIFDIVDPKTDEPVSGGE
RGELIATLLNHFTMPLIRYSLGDYVKNDFLTDPDPKYGITHMRFAEPIPGRVEWMFFVKGKLLLPIYVEDAVNEIPDTTG
MFNIFIYDNSMDKLKIRIETRREQVNSTYDKQAREILASRIGINPDDVEIEWVKPGNTIWTGYKLQVFVDQRKKK
>Mature_475_residues
MMSSVKNEYDAIHRELREQGFIRTDYPPNPSEVLWNKKIMTMKREEIDKLKTFRLRRIIKWAWENIEFYRRFWKSKNFEP
DMIKDWRDIAKIPILRKDEIRKDLQQNPPFGTIFHPELANHIRFVGATSGSTGLPTFQGWGALEMDYFQEAQARYLWTFA
EVKPTKVYANYLNMSGFYSWGPPVVETAMWRCGATVIAGGGETYFSWKNRHNLIFKLWKVDVFATTPWLHRLVGEEAKIE
GWETPFKVLLLHGGAAAENTKKKLFKVHPNAELAISVWGTTDGHMAIEVPSLEGQLVVWEDMEIFDIVDPKTDEPVSGGE
RGELIATLLNHFTMPLIRYSLGDYVKNDFLTDPDPKYGITHMRFAEPIPGRVEWMFFVKGKLLLPIYVEDAVNEIPDTTG
MFNIFIYDNSMDKLKIRIETRREQVNSTYDKQAREILASRIGINPDDVEIEWVKPGNTIWTGYKLQVFVDQRKKK

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=431, Percent_Identity=25.2900232018561, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 55389; Mature: 55389

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSSVKNEYDAIHRELREQGFIRTDYPPNPSEVLWNKKIMTMKREEIDKLKTFRLRRIIK
CCCCHHHHHHHHHHHHHHCCCEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
WAWENIEFYRRFWKSKNFEPDMIKDWRDIAKIPILRKDEIRKDLQQNPPFGTIFHPELAN
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEECHHHHC
HIRFVGATSGSTGLPTFQGWGALEMDYFQEAQARYLWTFAEVKPTKVYANYLNMSGFYSW
CEEEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHEEEEEECCCCHHHHHHHHCCCCCCCC
GPPVVETAMWRCGATVIAGGGETYFSWKNRHNLIFKLWKVDVFATTPWLHRLVGEEAKIE
CCHHHHHHHHHCCCEEEECCCCEEEEECCCCCEEEEEEEEEEEECCHHHHHHCCCCCCCC
GWETPFKVLLLHGGAAAENTKKKLFKVHPNAELAISVWGTTDGHMAIEVPSLEGQLVVWE
CCCCCEEEEEEECCCCCCCCCCCEEEECCCCEEEEEEECCCCCEEEEEECCCCCCEEEEE
DMEIFDIVDPKTDEPVSGGERGELIATLLNHFTMPLIRYSLGDYVKNDFLTDPDPKYGIT
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
HMRFAEPIPGRVEWMFFVKGKLLLPIYVEDAVNEIPDTTGMFNIFIYDNSMDKLKIRIET
EEEECCCCCCCEEEEEEECCCEEEEEEEHHHHHHCCCCCCEEEEEEEECCCCEEEEEEEH
RREQVNSTYDKQAREILASRIGINPDDVEIEWVKPGNTIWTGYKLQVFVDQRKKK
HHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECEEEEEEEECCCCC
>Mature Secondary Structure
MMSSVKNEYDAIHRELREQGFIRTDYPPNPSEVLWNKKIMTMKREEIDKLKTFRLRRIIK
CCCCHHHHHHHHHHHHHHCCCEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
WAWENIEFYRRFWKSKNFEPDMIKDWRDIAKIPILRKDEIRKDLQQNPPFGTIFHPELAN
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEECHHHHC
HIRFVGATSGSTGLPTFQGWGALEMDYFQEAQARYLWTFAEVKPTKVYANYLNMSGFYSW
CEEEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHEEEEEECCCCHHHHHHHHCCCCCCCC
GPPVVETAMWRCGATVIAGGGETYFSWKNRHNLIFKLWKVDVFATTPWLHRLVGEEAKIE
CCHHHHHHHHHCCCEEEECCCCEEEEECCCCCEEEEEEEEEEEECCHHHHHHCCCCCCCC
GWETPFKVLLLHGGAAAENTKKKLFKVHPNAELAISVWGTTDGHMAIEVPSLEGQLVVWE
CCCCCEEEEEEECCCCCCCCCCCEEEECCCCEEEEEEECCCCCEEEEEECCCCCCEEEEE
DMEIFDIVDPKTDEPVSGGERGELIATLLNHFTMPLIRYSLGDYVKNDFLTDPDPKYGIT
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
HMRFAEPIPGRVEWMFFVKGKLLLPIYVEDAVNEIPDTTGMFNIFIYDNSMDKLKIRIET
EEEECCCCCCCEEEEEEECCCEEEEEEEHHHHHHCCCCCCEEEEEEEECCCCEEEEEEEH
RREQVNSTYDKQAREILASRIGINPDDVEIEWVKPGNTIWTGYKLQVFVDQRKKK
HHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]