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The map label for this gene is yieO [C]

Identifier: 15898361

GI number: 15898361

Start: 1386468

End: 1387892

Strand: Reverse

Name: yieO [C]

Synonym: SSO1535

Alternate gene names: 15898361

Gene position: 1387892-1386468 (Counterclockwise)

Preceding gene: 15898364

Following gene: 15898359

Centisome position: 46.38

GC content: 35.72

Gene sequence:

>1425_bases
ATGGAGAGAGCATTTCTGAGATATGTCCCTATCCTAGCATTTATAACAATAATGACAATGTATGTAGAAATGGTAATTTT
GCCTTCTTTACCAGAAATAGAAAACCAATTCTCAATAACATCATCAGAAGCATCATGGGTCTTGTCTTCTGAAACATTAG
CTGGTATGACCTTAGCTCCCTTCTTAGGAAAATTAGCTGATAGTTATGGAAGGAAAAAGGTCTTAATTTCGATCCTAGTT
GTATATATACTAAGTGTATTCTTAACTGCGATCTCACCAAACTATTCCATCCTTTTAATTTCAAGGAGCATACAAGGGAT
AGGTTTAAGTATAAATCCAATAGCTTATACGATACTAAGGGAAAGAATACCGGATAGAGAACTACCAATAGCCCAAGGTA
TAATTGCATCAACATTTGCGATAGGTGCAGCTGTAGCACTTCCTATTGGTAGTTACATTTCACAATATTTCTCATGGGAA
TTCGCTTACGAAACAGCAATACCACTCTTAATTCTTTCTACTATAATTATCTATGTGATATTACCTTCATCCGAGTCAAA
GGGTAGTGATAGGAAAATAGACGATGTGGGAGTTATTCTTCTTTCCCTATCTTTCCTAATCCTTGGTATTGGTTTAACCG
AAGCTCCTACATGGGGATGGACATCTTTACAGTTCTTCACCTCTTTACTTCTCTCAATTCTTCTGTTTTACCTCTTTGCA
CTTCATATAAGCAAAAGTAGAAACCCGATAATTGACCCTTATGATTTTAAGAATCCTAATGTTGCTGTCCCACTTATTTC
CTCGTTTATTACTGGTTTTGGCTTATTCTTAACTTTCCAGTCTTTAGTCTTCATTTTTGAACTCCCGTCTCCAATAGGTT
ACGGTATGACTATTTTACAGACGGGAGAGACAATGGCACCAATTGCTTTAATAATTCTAATTGCTGGTCCCTTCTTTGGA
AGATTAGTCACAAAGATTGGCTATATGAAAGTAATCCTTACCTCGTCTTCAGTCTCATCTTTCCTTTTACTTTTCCTCGC
ATATATAATTTCCCTTAGAATTTCCGTTTTAGAATTAATGGGCTTCTTGTCATTAATCTTGTTTTTTATCTCTGGAATGA
ATGTGACAAGGGTAACTTTGCTAATCGCTTCATCAAAAAGAGAAAGGATGAGCACCTTAACTGGAACTAATACTTCAATG
AGGTTAATGGGTAATACTTTAGGTCCAATAGTTGTTGGGTCATTAGAGGATACATTTAAGACTCCAATATTTGAGGGGTA
TTTAAATAATATACCGATATTTGCATTTGTTCCTTCCAGATTGGCCTTTGAATATGCATTTTTAATCTCTATGGCTACAA
CTGTTTTAGTGACTATCTTAGCGACTAGGATAAGGGAAAGACCCTCTACTACAACCTATGCTTAA

Upstream 100 bases:

>100_bases
AATAATATTTCACTTTTCGTTTATACTGTACGATTAATGATCGTAAAAGTACTCTATTTGAGGAGTAAAAGTTTAGTATC
ATCAAGTAGATATATCTATT

Downstream 100 bases:

>100_bases
GTAATGAAAGCTAGACTGAATTTGAGACGAAAATTAACTTTTCATTTTTTAGTTTTTAAGCTGTGGTCAGCAGTTTCAGA
CGAACAGTAAAGTCTATAAA

Product: multidrug ABC transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MERAFLRYVPILAFITIMTMYVEMVILPSLPEIENQFSITSSEASWVLSSETLAGMTLAPFLGKLADSYGRKKVLISILV
VYILSVFLTAISPNYSILLISRSIQGIGLSINPIAYTILRERIPDRELPIAQGIIASTFAIGAAVALPIGSYISQYFSWE
FAYETAIPLLILSTIIIYVILPSSESKGSDRKIDDVGVILLSLSFLILGIGLTEAPTWGWTSLQFFTSLLLSILLFYLFA
LHISKSRNPIIDPYDFKNPNVAVPLISSFITGFGLFLTFQSLVFIFELPSPIGYGMTILQTGETMAPIALIILIAGPFFG
RLVTKIGYMKVILTSSSVSSFLLLFLAYIISLRISVLELMGFLSLILFFISGMNVTRVTLLIASSKRERMSTLTGTNTSM
RLMGNTLGPIVVGSLEDTFKTPIFEGYLNNIPIFAFVPSRLAFEYAFLISMATTVLVTILATRIRERPSTTTYA

Sequences:

>Translated_474_residues
MERAFLRYVPILAFITIMTMYVEMVILPSLPEIENQFSITSSEASWVLSSETLAGMTLAPFLGKLADSYGRKKVLISILV
VYILSVFLTAISPNYSILLISRSIQGIGLSINPIAYTILRERIPDRELPIAQGIIASTFAIGAAVALPIGSYISQYFSWE
FAYETAIPLLILSTIIIYVILPSSESKGSDRKIDDVGVILLSLSFLILGIGLTEAPTWGWTSLQFFTSLLLSILLFYLFA
LHISKSRNPIIDPYDFKNPNVAVPLISSFITGFGLFLTFQSLVFIFELPSPIGYGMTILQTGETMAPIALIILIAGPFFG
RLVTKIGYMKVILTSSSVSSFLLLFLAYIISLRISVLELMGFLSLILFFISGMNVTRVTLLIASSKRERMSTLTGTNTSM
RLMGNTLGPIVVGSLEDTFKTPIFEGYLNNIPIFAFVPSRLAFEYAFLISMATTVLVTILATRIRERPSTTTYA
>Mature_474_residues
MERAFLRYVPILAFITIMTMYVEMVILPSLPEIENQFSITSSEASWVLSSETLAGMTLAPFLGKLADSYGRKKVLISILV
VYILSVFLTAISPNYSILLISRSIQGIGLSINPIAYTILRERIPDRELPIAQGIIASTFAIGAAVALPIGSYISQYFSWE
FAYETAIPLLILSTIIIYVILPSSESKGSDRKIDDVGVILLSLSFLILGIGLTEAPTWGWTSLQFFTSLLLSILLFYLFA
LHISKSRNPIIDPYDFKNPNVAVPLISSFITGFGLFLTFQSLVFIFELPSPIGYGMTILQTGETMAPIALIILIAGPFFG
RLVTKIGYMKVILTSSSVSSFLLLFLAYIISLRISVLELMGFLSLILFFISGMNVTRVTLLIASSKRERMSTLTGTNTSM
RLMGNTLGPIVVGSLEDTFKTPIFEGYLNNIPIFAFVPSRLAFEYAFLISMATTVLVTILATRIRERPSTTTYA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=309, Percent_Identity=24.2718446601942, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1788710, Length=344, Percent_Identity=25.8720930232558, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789042, Length=324, Percent_Identity=23.1481481481481, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081983, Length=427, Percent_Identity=23.6533957845433, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788392, Length=369, Percent_Identity=21.9512195121951, Blast_Score=62, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323735, Length=148, Percent_Identity=26.3513513513513, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52279; Mature: 52279

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS00449 ATPASE_A ; PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERAFLRYVPILAFITIMTMYVEMVILPSLPEIENQFSITSSEASWVLSSETLAGMTLAP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEECCHHCCHHHHH
FLGKLADSYGRKKVLISILVVYILSVFLTAISPNYSILLISRSIQGIGLSINPIAYTILR
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEHHHHHHHHH
ERIPDRELPIAQGIIASTFAIGAAVALPIGSYISQYFSWEFAYETAIPLLILSTIIIYVI
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
LPSSESKGSDRKIDDVGVILLSLSFLILGIGLTEAPTWGWTSLQFFTSLLLSILLFYLFA
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LHISKSRNPIIDPYDFKNPNVAVPLISSFITGFGLFLTFQSLVFIFELPSPIGYGMTILQ
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
TGETMAPIALIILIAGPFFGRLVTKIGYMKVILTSSSVSSFLLLFLAYIISLRISVLELM
CCCCHHHHHHHHHHHCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
GFLSLILFFISGMNVTRVTLLIASSKRERMSTLTGTNTSMRLMGNTLGPIVVGSLEDTFK
HHHHHHHHHHCCCCHHEEEEEEECCHHHHHHHHCCCCCCHHHHCCCCCCEEECCCHHHHC
TPIFEGYLNNIPIFAFVPSRLAFEYAFLISMATTVLVTILATRIRERPSTTTYA
CCHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MERAFLRYVPILAFITIMTMYVEMVILPSLPEIENQFSITSSEASWVLSSETLAGMTLAP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEECCHHCCHHHHH
FLGKLADSYGRKKVLISILVVYILSVFLTAISPNYSILLISRSIQGIGLSINPIAYTILR
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEHHHHHHHHH
ERIPDRELPIAQGIIASTFAIGAAVALPIGSYISQYFSWEFAYETAIPLLILSTIIIYVI
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
LPSSESKGSDRKIDDVGVILLSLSFLILGIGLTEAPTWGWTSLQFFTSLLLSILLFYLFA
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LHISKSRNPIIDPYDFKNPNVAVPLISSFITGFGLFLTFQSLVFIFELPSPIGYGMTILQ
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
TGETMAPIALIILIAGPFFGRLVTKIGYMKVILTSSSVSSFLLLFLAYIISLRISVLELM
CCCCHHHHHHHHHHHCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
GFLSLILFFISGMNVTRVTLLIASSKRERMSTLTGTNTSMRLMGNTLGPIVVGSLEDTFK
HHHHHHHHHHCCCCHHEEEEEEECCHHHHHHHHCCCCCCHHHHCCCCCCEEECCCHHHHC
TPIFEGYLNNIPIFAFVPSRLAFEYAFLISMATTVLVTILATRIRERPSTTTYA
CCHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]