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The map label for this gene is gdhA-1 [H]

Identifier: 15898289

GI number: 15898289

Start: 1313283

End: 1314542

Strand: Direct

Name: gdhA-1 [H]

Synonym: SSO1457

Alternate gene names: 15898289

Gene position: 1313283-1314542 (Clockwise)

Preceding gene: 15898283

Following gene: 15898293

Centisome position: 43.89

GC content: 39.52

Gene sequence:

>1260_bases
ATGGAGGAACTTCTTACTTCGAATTTATTGGTTCAACAAGTTAAGAAGTTGTATAAAGTTGGTGAATTGTTGGGTTTAGA
TAGTGAGACTATAGATGTCCTTTCTCAACCAGAAAGGGTTATGCAAGTTAAGATACAGATTAGGAGTTCTGATGGTAAGT
TAAGGACGTTTATTGGTTGGAGAAGTCAGCATAATTCAGCCTTGGGTCCATATAAGGGAGGAGTTAGATACGGTCCCAAT
GTTACACAAGATGAAGTAATAGCACTATCGATGATTATGACGTGGAAGAACTCCCTTTTATTACTACCCTATGGGGGAGG
AAAGGGTGGGATAAGAGTAGATCCTAAGAAGCTAACATTAAAGGAATTGGAGGATTTGTCTAGGAAATACGTTCAATTGA
TTCACAAGTATTTGGGTAGTGATGTTGACATTCCTGCACCTGACATTAATACTAACCCGCAAACTATGGCTTGGTTCCTA
GATGAATACATAAAGATAACTGGTAAAGTAGATTTCGCAGTGTTTACTGGTAAGCCTTCCGAGCTAGGTGGGATAGGTGT
TAGACTCTACAGCACTGGGTTGGGTGTAGCGACAATAGCTAGAGATGCTGCTAATAAGTTTATAGGAGGAATTGAAGGAT
CAAGGGTTATAATTCAAGGGTTTGGAAACGTTGGTTTTTTTACCGCTAAATTCTTGAGCGAAATGGGGGCTAAAATAATT
GGAGTTAGCGATATTGGAGGAGGGGTAATTAATGAAAATGGAATAGACGTTAATAGGGCGTTGGAAGTTGTTCAGAGTAC
AGGTAGTGTTGTGAATTACCTTGAGGGTAAAAAGGTTACGAATGAGGAGTTGTTAATAAGCGATTGTGATATATTGATTC
CGGCTGCAGTGGAAAACGTTATAAATAAGTTTAATGCTCCGAAGGTTAGGGCAAAGCTTATTGTTGAAGGTGCAAATGGT
CCATTGACTGCAGATGCTGATGAAATAATGAAGCAAAGGGGTGTAATAGTCATACCAGACATTTTAGCTAACGCAGGGGG
AGTTGTGGGTAGTTATGTGGAGTGGGCTAACAATAGGTCTGGTGGAATAATTAGTGATGAGGAGGCTAAGAAACTTATTG
TAGATAGAATGATAAATGCTTTTAATACTTTATACGATTTTCACAAGAGAAAGTTTGCTGATCAGGATTTGAGGACTGTA
GCGATGGCTTTAGCTGTGGATAGAGTAGTAGGGGCAATGAAGGCGAGGGGTCTACTATAA

Upstream 100 bases:

>100_bases
GGAATCAATTTATCTTTTGCACTATAGGAAAATTGTGTATTAAAAAATTTTAAAATTAAGTTGGAATAATTTTTTATTCT
TAAATCATTAAGGGTAGAAC

Downstream 100 bases:

>100_bases
TATGTTCTTCTTTTCTCAGTCTTAAACTTATGCTAGGTATTAGACAGCTATTTTTTTTTTGTGTTTGTTCACCTATCAAA
TATTTTTTACTAAGAGCTAT

Product: NAD specific glutamate dehydrogenase (gdhA-1)

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MEELLTSNLLVQQVKKLYKVGELLGLDSETIDVLSQPERVMQVKIQIRSSDGKLRTFIGWRSQHNSALGPYKGGVRYGPN
VTQDEVIALSMIMTWKNSLLLLPYGGGKGGIRVDPKKLTLKELEDLSRKYVQLIHKYLGSDVDIPAPDINTNPQTMAWFL
DEYIKITGKVDFAVFTGKPSELGGIGVRLYSTGLGVATIARDAANKFIGGIEGSRVIIQGFGNVGFFTAKFLSEMGAKII
GVSDIGGGVINENGIDVNRALEVVQSTGSVVNYLEGKKVTNEELLISDCDILIPAAVENVINKFNAPKVRAKLIVEGANG
PLTADADEIMKQRGVIVIPDILANAGGVVGSYVEWANNRSGGIISDEEAKKLIVDRMINAFNTLYDFHKRKFADQDLRTV
AMALAVDRVVGAMKARGLL

Sequences:

>Translated_419_residues
MEELLTSNLLVQQVKKLYKVGELLGLDSETIDVLSQPERVMQVKIQIRSSDGKLRTFIGWRSQHNSALGPYKGGVRYGPN
VTQDEVIALSMIMTWKNSLLLLPYGGGKGGIRVDPKKLTLKELEDLSRKYVQLIHKYLGSDVDIPAPDINTNPQTMAWFL
DEYIKITGKVDFAVFTGKPSELGGIGVRLYSTGLGVATIARDAANKFIGGIEGSRVIIQGFGNVGFFTAKFLSEMGAKII
GVSDIGGGVINENGIDVNRALEVVQSTGSVVNYLEGKKVTNEELLISDCDILIPAAVENVINKFNAPKVRAKLIVEGANG
PLTADADEIMKQRGVIVIPDILANAGGVVGSYVEWANNRSGGIISDEEAKKLIVDRMINAFNTLYDFHKRKFADQDLRTV
AMALAVDRVVGAMKARGLL
>Mature_419_residues
MEELLTSNLLVQQVKKLYKVGELLGLDSETIDVLSQPERVMQVKIQIRSSDGKLRTFIGWRSQHNSALGPYKGGVRYGPN
VTQDEVIALSMIMTWKNSLLLLPYGGGKGGIRVDPKKLTLKELEDLSRKYVQLIHKYLGSDVDIPAPDINTNPQTMAWFL
DEYIKITGKVDFAVFTGKPSELGGIGVRLYSTGLGVATIARDAANKFIGGIEGSRVIIQGFGNVGFFTAKFLSEMGAKII
GVSDIGGGVINENGIDVNRALEVVQSTGSVVNYLEGKKVTNEELLISDCDILIPAAVENVINKFNAPKVRAKLIVEGANG
PLTADADEIMKQRGVIVIPDILANAGGVVGSYVEWANNRSGGIISDEEAKKLIVDRMINAFNTLYDFHKRKFADQDLRTV
AMALAVDRVVGAMKARGLL

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=341, Percent_Identity=39.2961876832845, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI4885281, Length=341, Percent_Identity=38.1231671554252, Blast_Score=249, Evalue=2e-66,
Organism=Escherichia coli, GI1788059, Length=343, Percent_Identity=33.8192419825073, Blast_Score=172, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17544676, Length=395, Percent_Identity=37.9746835443038, Blast_Score=251, Evalue=6e-67,
Organism=Saccharomyces cerevisiae, GI6324951, Length=396, Percent_Identity=29.040404040404, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6319256, Length=396, Percent_Identity=28.2828282828283, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24649283, Length=362, Percent_Identity=38.6740331491713, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI45553475, Length=342, Percent_Identity=39.4736842105263, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI45549226, Length=342, Percent_Identity=39.4736842105263, Blast_Score=250, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24649460, Length=279, Percent_Identity=40.5017921146953, Blast_Score=205, Evalue=4e-53,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 45510; Mature: 45510

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEELLTSNLLVQQVKKLYKVGELLGLDSETIDVLSQPERVMQVKIQIRSSDGKLRTFIGW
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEEE
RSQHNSALGPYKGGVRYGPNVTQDEVIALSMIMTWKNSLLLLPYGGGKGGIRVDPKKLTL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEECHHHHHH
KELEDLSRKYVQLIHKYLGSDVDIPAPDINTNPQTMAWFLDEYIKITGKVDFAVFTGKPS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCC
ELGGIGVRLYSTGLGVATIARDAANKFIGGIEGSRVIIQGFGNVGFFTAKFLSEMGAKII
CCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEE
GVSDIGGGVINENGIDVNRALEVVQSTGSVVNYLEGKKVTNEELLISDCDILIPAAVENV
EEECCCCCEECCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEECCCEEEHHHHHHH
INKFNAPKVRAKLIVEGANGPLTADADEIMKQRGVIVIPDILANAGGVVGSYVEWANNRS
HHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCEEECHHHCCCCCHHHHHHHHCCCCC
GGIISDEEAKKLIVDRMINAFNTLYDFHKRKFADQDLRTVAMALAVDRVVGAMKARGLL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEELLTSNLLVQQVKKLYKVGELLGLDSETIDVLSQPERVMQVKIQIRSSDGKLRTFIGW
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEEE
RSQHNSALGPYKGGVRYGPNVTQDEVIALSMIMTWKNSLLLLPYGGGKGGIRVDPKKLTL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEECHHHHHH
KELEDLSRKYVQLIHKYLGSDVDIPAPDINTNPQTMAWFLDEYIKITGKVDFAVFTGKPS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCC
ELGGIGVRLYSTGLGVATIARDAANKFIGGIEGSRVIIQGFGNVGFFTAKFLSEMGAKII
CCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEE
GVSDIGGGVINENGIDVNRALEVVQSTGSVVNYLEGKKVTNEELLISDCDILIPAAVENV
EEECCCCCEECCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEECCCEEEHHHHHHH
INKFNAPKVRAKLIVEGANGPLTADADEIMKQRGVIVIPDILANAGGVVGSYVEWANNRS
HHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCEEECHHHCCCCCHHHHHHHHCCCCC
GGIISDEEAKKLIVDRMINAFNTLYDFHKRKFADQDLRTVAMALAVDRVVGAMKARGLL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8125334 [H]