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The map label for this gene is aceA/icl [H]

Identifier: 15898176

GI number: 15898176

Start: 1168340

End: 1169653

Strand: Reverse

Name: aceA/icl [H]

Synonym: SSO1333

Alternate gene names: 15898176

Gene position: 1169653-1168340 (Counterclockwise)

Preceding gene: 15898177

Following gene: 15898175

Centisome position: 39.09

GC content: 40.11

Gene sequence:

>1314_bases
ATGAACATCCGAGATAAATGGATTGAAGAGGAGAAAAGTCTCGAATATGAGTGGTCTGAAGATCCAAGGTGGAGAGGAAT
AAAGAGAAATTATAAGCCCTTGGATGTGGTTAAATTAAGAGGATCTATTAGAATTGAGTATAGTTTAGCTAAGTTAGCCT
CTCACAAACTTTGGAACTTACTAAATACTGAGCCCTATGTGGCAACATTTGGGGCATTAACGGGTTCCCAAGCAGTGGAA
ATGGCAAAGGCGGGCCTTAAGGCTATTTACGTAAGTGGATGGCAAGTAGCTGCAGATAACAACTTATCAAATCAAACTTA
TCCAGACTTAAGCTTATACCCATCTAACAGCGTTCCAAACCTAGTAAAGAGGTTAAATAACGCCCTTATAAGGGCTGATC
AAATATCTTGGAGTGAGGGTAAACATGATATTGACTATTTACTGCCTATTGTTGCAGATGCTGAAGCTGGTTTTGGTGGT
CCAATTCACGCCTTCGAATTAACAAAAGCCTTAATAGAGGCAGGGGCTGCAGGGGTACATTTCGAAGATCAATTAGCAGC
AGAGAAGAAGTGTGGCCATTTAGGAGGGAAAGTTCTGATCCCTATAAGTGCATTTATAAGAGTACTAAACGCTGCAAGAC
TAGCGTCTGACGTATTAGGAGTACCAACAATACTAATAGCAAGGACAGACGCACTGAATGCTAAATACTTATCTAACGAT
GTAGATGAGACTGATAATCAATTCTTAACAGGAAAGAGGACATCAGAGGGATATTATGAGATAAAGGGTGGTATTGAATA
CGCCATAGCTAGAGGATTAGCTTATGCACCCTATGCTGACCTATTGTGGTTTGAGACTTCTAAGCCAGATTTGGAAGAGG
CTAGACAATTTGCTGAGGCTATTCACACTCATTATCCAGGTAAGATGTTAGCGTACAATTTATCCCCCTCATTTAACTGG
AAGAAATTTATGGATGATTCTAAAATTAGCAAGTTCATGAACGAATTGGGAGAAATGGGCTATAAATTCCAATTCATTAC
TCTTGCAGGTTGGCACTTAATTAACTACCATACATTCAAATTAGCGAGAGCATATAGAAATGAAGGAATGCCAGCATATG
TAAGACTACAAGAGTTAGAGTTCCAAGCACAAGCAGAAGGCTATACTGCAGTAAGCCACCAGAGAGAGGTTGGAACAGAT
TACTTTGACCTAGTGTTAACAATAGCGTCTGGGGGAGAAGCGTCCACTACTGCAATGGAAGGTTCCACTGAAGCTGAACA
GTTTGTAGAGGCAAAACAGAAAGTATTAAAATAG

Upstream 100 bases:

>100_bases
GAAGTTGATAAAATATCTTTAAATTGGATAGGGAAAGACATAAACTATTAATGCTAAAGTTTATATATTAAAATATGTGT
ATATAGATTAGGATGAAAAA

Downstream 100 bases:

>100_bases
CGGTGATTTAAGTATTCAATGTAAACCCACTTTATTTCAAAGATTGATCGAATTCAATTGGATGAAAAACTACTAATAGT
AGAGATGTATTATTACTAAT

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MNIRDKWIEEEKSLEYEWSEDPRWRGIKRNYKPLDVVKLRGSIRIEYSLAKLASHKLWNLLNTEPYVATFGALTGSQAVE
MAKAGLKAIYVSGWQVAADNNLSNQTYPDLSLYPSNSVPNLVKRLNNALIRADQISWSEGKHDIDYLLPIVADAEAGFGG
PIHAFELTKALIEAGAAGVHFEDQLAAEKKCGHLGGKVLIPISAFIRVLNAARLASDVLGVPTILIARTDALNAKYLSND
VDETDNQFLTGKRTSEGYYEIKGGIEYAIARGLAYAPYADLLWFETSKPDLEEARQFAEAIHTHYPGKMLAYNLSPSFNW
KKFMDDSKISKFMNELGEMGYKFQFITLAGWHLINYHTFKLARAYRNEGMPAYVRLQELEFQAQAEGYTAVSHQREVGTD
YFDLVLTIASGGEASTTAMEGSTEAEQFVEAKQKVLK

Sequences:

>Translated_437_residues
MNIRDKWIEEEKSLEYEWSEDPRWRGIKRNYKPLDVVKLRGSIRIEYSLAKLASHKLWNLLNTEPYVATFGALTGSQAVE
MAKAGLKAIYVSGWQVAADNNLSNQTYPDLSLYPSNSVPNLVKRLNNALIRADQISWSEGKHDIDYLLPIVADAEAGFGG
PIHAFELTKALIEAGAAGVHFEDQLAAEKKCGHLGGKVLIPISAFIRVLNAARLASDVLGVPTILIARTDALNAKYLSND
VDETDNQFLTGKRTSEGYYEIKGGIEYAIARGLAYAPYADLLWFETSKPDLEEARQFAEAIHTHYPGKMLAYNLSPSFNW
KKFMDDSKISKFMNELGEMGYKFQFITLAGWHLINYHTFKLARAYRNEGMPAYVRLQELEFQAQAEGYTAVSHQREVGTD
YFDLVLTIASGGEASTTAMEGSTEAEQFVEAKQKVLK
>Mature_437_residues
MNIRDKWIEEEKSLEYEWSEDPRWRGIKRNYKPLDVVKLRGSIRIEYSLAKLASHKLWNLLNTEPYVATFGALTGSQAVE
MAKAGLKAIYVSGWQVAADNNLSNQTYPDLSLYPSNSVPNLVKRLNNALIRADQISWSEGKHDIDYLLPIVADAEAGFGG
PIHAFELTKALIEAGAAGVHFEDQLAAEKKCGHLGGKVLIPISAFIRVLNAARLASDVLGVPTILIARTDALNAKYLSND
VDETDNQFLTGKRTSEGYYEIKGGIEYAIARGLAYAPYADLLWFETSKPDLEEARQFAEAIHTHYPGKMLAYNLSPSFNW
KKFMDDSKISKFMNELGEMGYKFQFITLAGWHLINYHTFKLARAYRNEGMPAYVRLQELEFQAQAEGYTAVSHQREVGTD
YFDLVLTIASGGEASTTAMEGSTEAEQFVEAKQKVLK

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=438, Percent_Identity=59.8173515981735, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1786525, Length=267, Percent_Identity=26.2172284644195, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71982926, Length=416, Percent_Identity=58.6538461538462, Blast_Score=497, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17561814, Length=416, Percent_Identity=58.6538461538462, Blast_Score=497, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6320908, Length=252, Percent_Identity=40.0793650793651, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6325263, Length=254, Percent_Identity=37.4015748031496, Blast_Score=167, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 48919; Mature: 48919

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRDKWIEEEKSLEYEWSEDPRWRGIKRNYKPLDVVKLRGSIRIEYSLAKLASHKLWNL
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHH
LNTEPYVATFGALTGSQAVEMAKAGLKAIYVSGWQVAADNNLSNQTYPDLSLYPSNSVPN
CCCCCCEEEHHCCCCHHHHHHHHCCCEEEEEECEEEEECCCCCCCCCCCCEECCCCCHHH
LVKRLNNALIRADQISWSEGKHDIDYLLPIVADAEAGFGGPIHAFELTKALIEAGAAGVH
HHHHHHHHHEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
FEDQLAAEKKCGHLGGKVLIPISAFIRVLNAARLASDVLGVPTILIARTDALNAKYLSND
CHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCC
VDETDNQFLTGKRTSEGYYEIKGGIEYAIARGLAYAPYADLLWFETSKPDLEEARQFAEA
CCCCCCCEECCCCCCCCEEEECCCHHHHHHCCCCCCCCHHEEEEECCCCCHHHHHHHHHH
IHTHYPGKMLAYNLSPSFNWKKFMDDSKISKFMNELGEMGYKFQFITLAGWHLINYHTFK
HHHCCCCCEEEEECCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEEEEECEEEEEHHHHH
LARAYRNEGMPAYVRLQELEFQAQAEGYTAVSHQREVGTDYFDLVLTIASGGEASTTAME
HHHHHHCCCCCEEEEEEHHHHHHHCCCCCHHHHHHHHCCCHHHEEEEEECCCCCCCEEEC
GSTEAEQFVEAKQKVLK
CCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIRDKWIEEEKSLEYEWSEDPRWRGIKRNYKPLDVVKLRGSIRIEYSLAKLASHKLWNL
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHH
LNTEPYVATFGALTGSQAVEMAKAGLKAIYVSGWQVAADNNLSNQTYPDLSLYPSNSVPN
CCCCCCEEEHHCCCCHHHHHHHHCCCEEEEEECEEEEECCCCCCCCCCCCEECCCCCHHH
LVKRLNNALIRADQISWSEGKHDIDYLLPIVADAEAGFGGPIHAFELTKALIEAGAAGVH
HHHHHHHHHEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
FEDQLAAEKKCGHLGGKVLIPISAFIRVLNAARLASDVLGVPTILIARTDALNAKYLSND
CHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCC
VDETDNQFLTGKRTSEGYYEIKGGIEYAIARGLAYAPYADLLWFETSKPDLEEARQFAEA
CCCCCCCEECCCCCCCCEEEECCCHHHHHHCCCCCCCCHHEEEEECCCCCHHHHHHHHHH
IHTHYPGKMLAYNLSPSFNWKKFMDDSKISKFMNELGEMGYKFQFITLAGWHLINYHTFK
HHHCCCCCEEEEECCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEEEEECEEEEEHHHHH
LARAYRNEGMPAYVRLQELEFQAQAEGYTAVSHQREVGTDYFDLVLTIASGGEASTTAME
HHHHHHCCCCCEEEEEEHHHHHHHCCCCCHHHHHHHHCCCHHHEEEEEECCCCCCCEEEC
GSTEAEQFVEAKQKVLK
CCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]