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The map label for this gene is paaF-3 [C]

Identifier: 15898152

GI number: 15898152

Start: 1140958

End: 1141761

Strand: Reverse

Name: paaF-3 [C]

Synonym: SSO1311

Alternate gene names: 15898152

Gene position: 1141761-1140958 (Counterclockwise)

Preceding gene: 15898153

Following gene: 15898147

Centisome position: 38.16

GC content: 37.44

Gene sequence:

>804_bases
ATGGGTTTAACTGAATTGACTCCAAAATATTTCAAAATAGAAGTCGAAGATGGAGTGGGGATAATAAGATTAAATAGACC
ACCAGCTAATGCACATAATCTGGAAATGTTAAGAGAATTGGATAACATTATTGTGGAGTCACGTTTTGATGAAAATGTAA
AAGCGTTAATTATAACTAGCGCAATTCCCAGATTTTTCTCTGCAGGATTTGATATAAATGAAATAAAGGATAAATCACCA
GAATACATAGGGCTCTCAAGTCAGTTTAGCAAAGAGGTCATGTTAAGAATGATGTCAACAAAGAAATTGATAATAGCCAG
CATAAATGGCCATTGTATGGGTGGGGGCTTAGAACTGGCACTTGCTTGTGATTTGAGGTTCGGTGCCAATGATGAGAATA
TTAAATTTGGAATGCCAGAAGTAGCCAATTTAGCTTTAATCCCAGGTGAGGGAGGAACTCAGTTTTTAGCTAGATTAGTT
GGAAGATCGAAGGCTATATATCTAATGATAACCGGCAAGACACTTTCTCCTAAAGAGGCTTATGATTTAGGTATCCTAGA
CAAGTTAGTAGAGCCAGAAAAACTATTTGAAGAGTCATTAGAGTTTGCTAGGCAAATATCTAGAGGTCCATCATTAGCAG
TGGGATTTGCAAAATTAGCAGTTAATGAAGGAATGGATTTGCCGTTGTATAATGCTTTTGCTCTGGAGAGAGAAATGCAG
AATCAAGCTCTGGCTTCAGAGGATGCTAAGGAAGGCGCTAGAGCTTTCTTCGAGAAAAGAAGACCTAATTTTAAAGGGAA
GTGA

Upstream 100 bases:

>100_bases
AGGCAATTATACATAAAAGATGTCGCAGAATTAATCGCACCCTTAGGTTTAGAGTTACCAGATGTGAATAGGAATAGAAG
AATATTTTAGGTGATAGTTC

Downstream 100 bases:

>100_bases
AACTTCCTAACCTCATAAGATTTTTATATTCCAGATTTGATACAATTGGTAATTTTAGATTGTAATTATTAAAGTTAATA
TTTACTATTATTTTAATATT

Product: enoyl CoA hydratase (paaF-3)

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MGLTELTPKYFKIEVEDGVGIIRLNRPPANAHNLEMLRELDNIIVESRFDENVKALIITSAIPRFFSAGFDINEIKDKSP
EYIGLSSQFSKEVMLRMMSTKKLIIASINGHCMGGGLELALACDLRFGANDENIKFGMPEVANLALIPGEGGTQFLARLV
GRSKAIYLMITGKTLSPKEAYDLGILDKLVEPEKLFEESLEFARQISRGPSLAVGFAKLAVNEGMDLPLYNAFALEREMQ
NQALASEDAKEGARAFFEKRRPNFKGK

Sequences:

>Translated_267_residues
MGLTELTPKYFKIEVEDGVGIIRLNRPPANAHNLEMLRELDNIIVESRFDENVKALIITSAIPRFFSAGFDINEIKDKSP
EYIGLSSQFSKEVMLRMMSTKKLIIASINGHCMGGGLELALACDLRFGANDENIKFGMPEVANLALIPGEGGTQFLARLV
GRSKAIYLMITGKTLSPKEAYDLGILDKLVEPEKLFEESLEFARQISRGPSLAVGFAKLAVNEGMDLPLYNAFALEREMQ
NQALASEDAKEGARAFFEKRRPNFKGK
>Mature_266_residues
GLTELTPKYFKIEVEDGVGIIRLNRPPANAHNLEMLRELDNIIVESRFDENVKALIITSAIPRFFSAGFDINEIKDKSPE
YIGLSSQFSKEVMLRMMSTKKLIIASINGHCMGGGLELALACDLRFGANDENIKFGMPEVANLALIPGEGGTQFLARLVG
RSKAIYLMITGKTLSPKEAYDLGILDKLVEPEKLFEESLEFARQISRGPSLAVGFAKLAVNEGMDLPLYNAFALEREMQN
QALASEDAKEGARAFFEKRRPNFKGK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=36.6935483870968, Blast_Score=147, Evalue=7e-36,
Organism=Homo sapiens, GI20127408, Length=190, Percent_Identity=33.1578947368421, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI68989263, Length=196, Percent_Identity=36.2244897959184, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=34.375, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI31542718, Length=265, Percent_Identity=29.0566037735849, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI62530384, Length=233, Percent_Identity=26.6094420600858, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI213417737, Length=230, Percent_Identity=29.5652173913043, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI157694516, Length=230, Percent_Identity=29.5652173913043, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI295842266, Length=233, Percent_Identity=25.3218884120172, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI150378541, Length=258, Percent_Identity=27.5193798449612, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI157694520, Length=208, Percent_Identity=28.8461538461538, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI261878539, Length=97, Percent_Identity=44.3298969072165, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI37594471, Length=195, Percent_Identity=34.8717948717949, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI37594469, Length=195, Percent_Identity=34.8717948717949, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI70995211, Length=253, Percent_Identity=24.5059288537549, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=33.59375, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1788682, Length=192, Percent_Identity=35.9375, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI221142681, Length=251, Percent_Identity=31.4741035856574, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=33.976833976834, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=26.8482490272374, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI87082183, Length=206, Percent_Identity=29.6116504854369, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1790281, Length=176, Percent_Identity=30.1136363636364, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17554946, Length=267, Percent_Identity=34.8314606741573, Blast_Score=147, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=31.7269076305221, Blast_Score=123, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17540714, Length=232, Percent_Identity=34.4827586206897, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17558304, Length=214, Percent_Identity=31.3084112149533, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17508953, Length=184, Percent_Identity=33.695652173913, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25144276, Length=184, Percent_Identity=33.695652173913, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17508951, Length=184, Percent_Identity=33.695652173913, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25141363, Length=239, Percent_Identity=25.1046025104602, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17560910, Length=200, Percent_Identity=27.5, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=262, Percent_Identity=25.9541984732824, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI25144160, Length=184, Percent_Identity=32.6086956521739, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144157, Length=184, Percent_Identity=32.6086956521739, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=240, Percent_Identity=29.1666666666667, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI20129971, Length=260, Percent_Identity=35.3846153846154, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24653477, Length=260, Percent_Identity=35.3846153846154, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24653139, Length=258, Percent_Identity=33.7209302325581, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19921000, Length=187, Percent_Identity=35.8288770053476, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24583077, Length=187, Percent_Identity=35.8288770053476, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24583079, Length=187, Percent_Identity=35.8288770053476, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19922422, Length=256, Percent_Identity=25.78125, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19921018, Length=220, Percent_Identity=29.0909090909091, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21357171, Length=269, Percent_Identity=25.6505576208178, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24583165, Length=213, Percent_Identity=29.1079812206573, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571730, Length=185, Percent_Identity=35.1351351351351, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28571729, Length=185, Percent_Identity=35.1351351351351, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24654903, Length=216, Percent_Identity=23.6111111111111, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550169, Length=185, Percent_Identity=26.4864864864865, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29595; Mature: 29464

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLTELTPKYFKIEVEDGVGIIRLNRPPANAHNLEMLRELDNIIVESRFDENVKALIITS
CCCCCCCCCEEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEHH
AIPRFFSAGFDINEIKDKSPEYIGLSSQFSKEVMLRMMSTKKLIIASINGHCMGGGLELA
HHHHHHHCCCCHHHHCCCCCCEECCHHHHHHHHHHHHHCCCEEEEEECCCEEECCCEEEE
LACDLRFGANDENIKFGMPEVANLALIPGEGGTQFLARLVGRSKAIYLMITGKTLSPKEA
EEEEEEECCCCCCEEECCCCCCCEEEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHH
YDLGILDKLVEPEKLFEESLEFARQISRGPSLAVGFAKLAVNEGMDLPLYNAFALEREMQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
NQALASEDAKEGARAFFEKRRPNFKGK
HHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GLTELTPKYFKIEVEDGVGIIRLNRPPANAHNLEMLRELDNIIVESRFDENVKALIITS
CCCCCCCCEEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEHH
AIPRFFSAGFDINEIKDKSPEYIGLSSQFSKEVMLRMMSTKKLIIASINGHCMGGGLELA
HHHHHHHCCCCHHHHCCCCCCEECCHHHHHHHHHHHHHCCCEEEEEECCCEEECCCEEEE
LACDLRFGANDENIKFGMPEVANLALIPGEGGTQFLARLVGRSKAIYLMITGKTLSPKEA
EEEEEEECCCCCCEEECCCCCCCEEEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHH
YDLGILDKLVEPEKLFEESLEFARQISRGPSLAVGFAKLAVNEGMDLPLYNAFALEREMQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
NQALASEDAKEGARAFFEKRRPNFKGK
HHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA