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The map label for this gene is bioB

Identifier: 15897976

GI number: 15897976

Start: 961609

End: 962664

Strand: Direct

Name: bioB

Synonym: SSO1115

Alternate gene names: NA

Gene position: 961609-962664 (Clockwise)

Preceding gene: 15897975

Following gene: 15897977

Centisome position: 32.14

GC content: 39.3

Gene sequence:

>1056_bases
ATGACATTACGTCTAATTTCAAGTCCAGATTGGGTAAGACTAAGTTTTGGCGCAGATATGGTCTTAGGTTTTTCTTCTGG
AGTGTTTTTAAAGGGAGCTCTGAATACCACGATAAATCTGTTGCAATATTACCCAGATGGATGCAAAGCAAATTGCTCAT
ATTGTGGCCAAGCTAGAGAGGTCGCCAATGGTCCAGAATGTAAAACGTTAATAAGGGTAGAATGGCCACTTAGACCTCTA
AATGAGGTATTAAAGAGAATTTATGAGAGACAAGGCAATCCAGAATACGGTCTTCAAAGGATATGTATAGGACAATTGGC
CCATCCTAGGGCATCTCCAGATGCCATAGAGATAACGAGAAGGATAAGAAATGCGGGGATTGAGCTACAAATTTCAGAAT
TAGTTACAGCAACTTATACTTTCAAACATCACATGATTGAAATGAGGAAAGCTGGAGCTGATATGATTGATGTTGCAATA
GACGCGGCAAATGAGAAGGTGTTTGAAGAGCTAAGGGGTAAGAAAGCTAGAAGCATGCACTCATGGAAGAGATATTTAGA
GGCAATAGATGAAGCTGTGGAAGTATTTGGTAAAAAGAACGCTGGTATTCACTTAATAATAGGTTTAGGGGAGAGTGAGA
AAGACGCAGTCAACCTTATGTGGTACGCTCATAGTAGGGGAGCTAAAATTTCACTCTTTGCGTTTTATCCAGAGGCTGGT
ACTCCAATGGAAAAACGAAAACCAGTCCCAGTTCACGTCTATAGAAGGATGCAAATAGCTAGATGGCTGATTGAAAATGA
TATTGTCGATATTAATGCGTTCAAGTTTGACGACAAGGGAGAACTAATAGATGTAGAAATACCGTCCGATATAACCTTAA
ATGAATTAGCCCCAGCATTTATGACTAGTGGATGTCCAGGTTGTAACAGACCATATTCCAATGAGAGGCCAGGTAGAGTA
TTAAAGAACGTTCCTTGGTATCTAGACAGAGAAATGACTTTACGTTCAATTAAAGCTTCCAGATTAGATTCCTTAATAAA
GAAAGTAGTAAGGTAA

Upstream 100 bases:

>100_bases
AAGACGAAACGTACGAATACGCAAAAAGTAAGAGGGAAATATTTTTAAGTAATGCGTGTTGTGGTAATATAGTTTTTGAT
ATATTTAAGGTGATAACCTC

Downstream 100 bases:

>100_bases
AAATGTTCATAAAGGATGGCTCTTTAATAAGCTATTTTTCAAGTGGTTTTCCTTCACATGTTAGAGTTAAGGCAATAGAT
ATCTCATCTCCAGACTATGA

Product: biotin synthase (bioB)

Products: biotin

Alternate protein names: Radical SAM Domain-Containing Protein; Biotin Synthase Related Protein; Biotin Synthase; Biotin Synthase-Related Protein; Biotin Synthase-Related; Radical SAM Family Protein; Radical SAM Superfamily Protein

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTLRLISSPDWVRLSFGADMVLGFSSGVFLKGALNTTINLLQYYPDGCKANCSYCGQAREVANGPECKTLIRVEWPLRPL
NEVLKRIYERQGNPEYGLQRICIGQLAHPRASPDAIEITRRIRNAGIELQISELVTATYTFKHHMIEMRKAGADMIDVAI
DAANEKVFEELRGKKARSMHSWKRYLEAIDEAVEVFGKKNAGIHLIIGLGESEKDAVNLMWYAHSRGAKISLFAFYPEAG
TPMEKRKPVPVHVYRRMQIARWLIENDIVDINAFKFDDKGELIDVEIPSDITLNELAPAFMTSGCPGCNRPYSNERPGRV
LKNVPWYLDREMTLRSIKASRLDSLIKKVVR

Sequences:

>Translated_351_residues
MTLRLISSPDWVRLSFGADMVLGFSSGVFLKGALNTTINLLQYYPDGCKANCSYCGQAREVANGPECKTLIRVEWPLRPL
NEVLKRIYERQGNPEYGLQRICIGQLAHPRASPDAIEITRRIRNAGIELQISELVTATYTFKHHMIEMRKAGADMIDVAI
DAANEKVFEELRGKKARSMHSWKRYLEAIDEAVEVFGKKNAGIHLIIGLGESEKDAVNLMWYAHSRGAKISLFAFYPEAG
TPMEKRKPVPVHVYRRMQIARWLIENDIVDINAFKFDDKGELIDVEIPSDITLNELAPAFMTSGCPGCNRPYSNERPGRV
LKNVPWYLDREMTLRSIKASRLDSLIKKVVR
>Mature_350_residues
TLRLISSPDWVRLSFGADMVLGFSSGVFLKGALNTTINLLQYYPDGCKANCSYCGQAREVANGPECKTLIRVEWPLRPLN
EVLKRIYERQGNPEYGLQRICIGQLAHPRASPDAIEITRRIRNAGIELQISELVTATYTFKHHMIEMRKAGADMIDVAID
AANEKVFEELRGKKARSMHSWKRYLEAIDEAVEVFGKKNAGIHLIIGLGESEKDAVNLMWYAHSRGAKISLFAFYPEAGT
PMEKRKPVPVHVYRRMQIARWLIENDIVDINAFKFDDKGELIDVEIPSDITLNELAPAFMTSGCPGCNRPYSNERPGRVL
KNVPWYLDREMTLRSIKASRLDSLIKKVVR

Specific function: Unknown

COG id: COG2516

COG function: function code R; Biotin synthase-related enzyme

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.8.1.6

Molecular weight: Translated: 39815; Mature: 39683

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRLISSPDWVRLSFGADMVLGFSSGVFLKGALNTTINLLQYYPDGCKANCSYCGQARE
CEEEEECCCCEEEEECCCCEEEECCCCEEEEEHHHHHHHHHHHCCCCCCCCCHHCCHHHH
VANGPECKTLIRVEWPLRPLNEVLKRIYERQGNPEYGLQRICIGQLAHPRASPDAIEITR
HCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHH
RIRNAGIELQISELVTATYTFKHHMIEMRKAGADMIDVAIDAANEKVFEELRGKKARSMH
HHHHCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCHHHHHHH
SWKRYLEAIDEAVEVFGKKNAGIHLIIGLGESEKDAVNLMWYAHSRGAKISLFAFYPEAG
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCEEEEEEEECCCC
TPMEKRKPVPVHVYRRMQIARWLIENDIVDINAFKFDDKGELIDVEIPSDITLNELAPAF
CCHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHHHH
MTSGCPGCNRPYSNERPGRVLKNVPWYLDREMTLRSIKASRLDSLIKKVVR
HHCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TLRLISSPDWVRLSFGADMVLGFSSGVFLKGALNTTINLLQYYPDGCKANCSYCGQARE
EEEEECCCCEEEEECCCCEEEECCCCEEEEEHHHHHHHHHHHCCCCCCCCCHHCCHHHH
VANGPECKTLIRVEWPLRPLNEVLKRIYERQGNPEYGLQRICIGQLAHPRASPDAIEITR
HCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHH
RIRNAGIELQISELVTATYTFKHHMIEMRKAGADMIDVAIDAANEKVFEELRGKKARSMH
HHHHCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCHHHHHHH
SWKRYLEAIDEAVEVFGKKNAGIHLIIGLGESEKDAVNLMWYAHSRGAKISLFAFYPEAG
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCEEEEEEEECCCC
TPMEKRKPVPVHVYRRMQIARWLIENDIVDINAFKFDDKGELIDVEIPSDITLNELAPAF
CCHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHHHH
MTSGCPGCNRPYSNERPGRVLKNVPWYLDREMTLRSIKASRLDSLIKKVVR
HHCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dethiobiotin; sulfur

Specific reaction: dethiobiotin + sulfur = biotin

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA