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The map label for this gene is dphB

Identifier: 15897832

GI number: 15897832

Start: 808432

End: 809205

Strand: Direct

Name: dphB

Synonym: SSO0953

Alternate gene names: 15897832

Gene position: 808432-809205 (Clockwise)

Preceding gene: 15897829

Following gene: 15897833

Centisome position: 27.02

GC content: 33.59

Gene sequence:

>774_bases
GTGAGCATCTTAAGTCTAGTAGGTCTTGGAATCTCTAAGAAATTCATAACTGATAGTGCTATAGAGACACTAAGTAATTC
TGATATTATAATTTTTGATAGATATACATCTAGATCTTGTGATATAAATGTGGACGTATTAAGGAGATTGGTAAAGGGAG
AGAGAGAATTCATTGAAGCGGATAGGAGTTTATTAGAAAATAACTCAAAGGCTATCATAGACTATTTAGACAAGGGTTAT
AATGTAAGTATAGCCAGTATTGGAGATGCATTAATAGCCACAACCCATGTTTCGCTACTTATAGAGGCTAAACATCGAGG
ACATGAGGTAAAAGTGATTCCAGGGATTTCAGTACATTGCTATCTTATATCAAAGTCTCTATTATCTTCCTATAAATTTG
GTAAGTCAGTTACAGTTACATTTCCTTATAACGATTTCATAGATCCTACTCCCTATAATGTGATTAAGGATAACAAAGAA
AGAGGTCTTCATACCATATTATATCTGGATTTGAAAAACGAGAAAGCAATGACTGCTAACGAAGCACTTCAGATCTTATT
ACGACTTGAAGAGAGGCATAAGAAAAGTGTTCTTTCAAAATCTGATATTATAATAGTTGGTGCTAGATTAGGGTGTGATG
ACGAGAGAATTATTGCACTTAAAGTAGAAGAAGCTACTTCATTCGACTTCGGAAATACACCACATATTATTATAATTCCT
GGTAACCTTCATTATATGGAGGCTGATGCTATAAAATGGATATTGAGGAGTTAA

Upstream 100 bases:

>100_bases
TTACGTAAAGTGCTGGTAAGTCCTCAGCAATTAAGATTCCTTTAGCGAGTTCAAGCATTTAAATAAGATAATATTAAACA
CTATTTTATACATTTTTTCT

Downstream 100 bases:

>100_bases
AGAAAAGGATAGAAAAGTACATTAAAGGAATGGATGAACGATTAAAGGACACTAGTAGTGCCAACAATAAGGTAGTAGAA
TTGGCTAGATTATATACTGA

Product: diphthine synthase

Products: NA

Alternate protein names: Diphthamide biosynthesis methyltransferase

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSILSLVGLGISKKFITDSAIETLSNSDIIIFDRYTSRSCDINVDVLRRLVKGEREFIEADRSLLENNSKAIIDYLDKGY
NVSIASIGDALIATTHVSLLIEAKHRGHEVKVIPGISVHCYLISKSLLSSYKFGKSVTVTFPYNDFIDPTPYNVIKDNKE
RGLHTILYLDLKNEKAMTANEALQILLRLEERHKKSVLSKSDIIIVGARLGCDDERIIALKVEEATSFDFGNTPHIIIIP
GNLHYMEADAIKWILRS

Sequences:

>Translated_257_residues
MSILSLVGLGISKKFITDSAIETLSNSDIIIFDRYTSRSCDINVDVLRRLVKGEREFIEADRSLLENNSKAIIDYLDKGY
NVSIASIGDALIATTHVSLLIEAKHRGHEVKVIPGISVHCYLISKSLLSSYKFGKSVTVTFPYNDFIDPTPYNVIKDNKE
RGLHTILYLDLKNEKAMTANEALQILLRLEERHKKSVLSKSDIIIVGARLGCDDERIIALKVEEATSFDFGNTPHIIIIP
GNLHYMEADAIKWILRS
>Mature_256_residues
SILSLVGLGISKKFITDSAIETLSNSDIIIFDRYTSRSCDINVDVLRRLVKGEREFIEADRSLLENNSKAIIDYLDKGYN
VSIASIGDALIATTHVSLLIEAKHRGHEVKVIPGISVHCYLISKSLLSSYKFGKSVTVTFPYNDFIDPTPYNVIKDNKER
GLHTILYLDLKNEKAMTANEALQILLRLEERHKKSVLSKSDIIIVGARLGCDDERIIALKVEEATSFDFGNTPHIIIIPG
NLHYMEADAIKWILRS

Specific function: Required for the methylation step in diphthamide biosynthesis

COG id: COG1798

COG function: function code J; Diphthamide biosynthesis methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diphthine synthase family

Homologues:

Organism=Homo sapiens, GI117190326, Length=272, Percent_Identity=26.1029411764706, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI117190262, Length=272, Percent_Identity=26.1029411764706, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI117190324, Length=271, Percent_Identity=25.830258302583, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17531369, Length=273, Percent_Identity=28.9377289377289, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6323201, Length=257, Percent_Identity=27.2373540856031, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28572120, Length=274, Percent_Identity=25.9124087591241, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DPHB_SULSO (Q97TX8)

Other databases:

- EMBL:   AE006641
- PIR:   D90246
- RefSeq:   NP_342437.1
- ProteinModelPortal:   Q97TX8
- SMR:   Q97TX8
- GeneID:   1455194
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0953
- NMPDR:   fig|273057.1.peg.860
- HOGENOM:   HBG460511
- OMA:   YAEFYTA
- PhylomeDB:   Q97TX8
- ProtClustDB:   PRK04160
- BioCyc:   SSOL273057:SSO0953-MONOMER
- BRENDA:   2.1.1.98
- HAMAP:   MF_01084
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR004551
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- PANTHER:   PTHR10882
- PIRSF:   PIRSF036432
- TIGRFAMs:   TIGR00522

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.98

Molecular weight: Translated: 28835; Mature: 28703

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILSLVGLGISKKFITDSAIETLSNSDIIIFDRYTSRSCDINVDVLRRLVKGEREFIEA
CCHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHCHHHHHHH
DRSLLENNSKAIIDYLDKGYNVSIASIGDALIATTHVSLLIEAKHRGHEVKVIPGISVHC
HHHHHHCCCCEEEHHHCCCCEEEEHHHCCHHHHHHEEEEEEEECCCCCEEEEECCCEEEE
YLISKSLLSSYKFGKSVTVTFPYNDFIDPTPYNVIKDNKERGLHTILYLDLKNEKAMTAN
EEEEHHHHHHHCCCCEEEEEECCCCCCCCCCCHHCCCCCCCCEEEEEEEEECCCCCCCHH
EALQILLRLEERHKKSVLSKSDIIIVGARLGCDDERIIALKVEEATSFDFGNTPHIIIIP
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCEEEEEC
GNLHYMEADAIKWILRS
CCEEEECHHHHHHHHCC
>Mature Secondary Structure 
SILSLVGLGISKKFITDSAIETLSNSDIIIFDRYTSRSCDINVDVLRRLVKGEREFIEA
CHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHCHHHHHHH
DRSLLENNSKAIIDYLDKGYNVSIASIGDALIATTHVSLLIEAKHRGHEVKVIPGISVHC
HHHHHHCCCCEEEHHHCCCCEEEEHHHCCHHHHHHEEEEEEEECCCCCEEEEECCCEEEE
YLISKSLLSSYKFGKSVTVTFPYNDFIDPTPYNVIKDNKERGLHTILYLDLKNEKAMTAN
EEEEHHHHHHHCCCCEEEEEECCCCCCCCCCCHHCCCCCCCCEEEEEEEEECCCCCCCHH
EALQILLRLEERHKKSVLSKSDIIIVGARLGCDDERIIALKVEEATSFDFGNTPHIIIIP
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCEEEEEC
GNLHYMEADAIKWILRS
CCEEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726