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The map label for this gene is speE [H]

Identifier: 15897656

GI number: 15897656

Start: 641071

End: 641976

Strand: Reverse

Name: speE [H]

Synonym: SSO0757

Alternate gene names: 15897656

Gene position: 641976-641071 (Counterclockwise)

Preceding gene: 15897657

Following gene: 15897654

Centisome position: 21.45

GC content: 35.21

Gene sequence:

>906_bases
ATGTTTGGTTGGCATTGGCTCCTAGAATGGCAAACGCCATATGAGTTTCACGGTCACCTAATAGAAAAAGTATTAGCTGA
AGAGAAGACTCCATATCAGCATGTTACATTAGTTGAATTCACTAGGTTTGGAAAAGGGCTTATCATAGATGGAAAAGTGC
AATCAACACTATATGATGAGCATATTTATCATGAATTATTAGTTCATCCATTATTACTATCATTAACAAAACCTCCTAAG
AGTGTGTTAATTTTAGGCGGTGGAGAAGGAGCAACGCTTAGAGAAGTTCTTAAGTATAAGTCTGTAGAAAAAGCAGTAAT
GGTTGATATAGACGAGAAGGTTATAGAGTTTGCTAAGAAGTATCTATATGAGTGGCATCAGGGAGCTTTTGAGGATAAGA
GGACTAACCTAGTAATTACTGATGGTTTAAAGTTTATAAAAGAAACAAAGGAGAAATATGATGCAATAATTCTAGACCTA
ACAGATCCAATCAAGAATTCTACATCATATATGCTTTATACTAAGGAGTTCTATGAGAAATTAAGAGAAATATTGAATGA
GAGAGGAGGCATAGTAACACAAGCCACATCTCCATCATTTTCTCTAGAAGTATATGTGACGATTTATAACACTATAAAGG
AGGTATTTAAGGAAGCATCAGCTTCATACACCTATATGGCATCCTTTGATGGATTATGGGGATTCGTCTATGGAGGAGTA
AGGCCAGACCTACTTTCGGAGGATGAAGTAGATTCCAAAATAAACGAACGTATTGATGGACAGCTAAGATTCTATGACGG
CTATTCTCATAAGATTTCGTTTAGTTTGCCTAAGAATATAAAAAGTGAATTCCAGAAGATAACTAAAATATCCACAGAAA
AAGATCCGATTTACGTTCCTGCTTAA

Upstream 100 bases:

>100_bases
TAAGTAAGCACTACTGTGCAGCTTGTGGATTTGGAAGGACGAAGAAGATAAGGAGATATAGCTGGCAAAATAAGAAGGTT
AATGGGGTCAGAATAAGATA

Downstream 100 bases:

>100_bases
TTTTTTAAATTTTTATCGATATATATTTAATTGCCGGGGTGCCCGAGCGGTCAAGGGGGTAGGCTCGAGACCTTTCCAGC
GTTACTGCGAGGGACCTACT

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MFGWHWLLEWQTPYEFHGHLIEKVLAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDEHIYHELLVHPLLLSLTKPPK
SVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKKYLYEWHQGAFEDKRTNLVITDGLKFIKETKEKYDAIILDL
TDPIKNSTSYMLYTKEFYEKLREILNERGGIVTQATSPSFSLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGV
RPDLLSEDEVDSKINERIDGQLRFYDGYSHKISFSLPKNIKSEFQKITKISTEKDPIYVPA

Sequences:

>Translated_301_residues
MFGWHWLLEWQTPYEFHGHLIEKVLAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDEHIYHELLVHPLLLSLTKPPK
SVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKKYLYEWHQGAFEDKRTNLVITDGLKFIKETKEKYDAIILDL
TDPIKNSTSYMLYTKEFYEKLREILNERGGIVTQATSPSFSLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGV
RPDLLSEDEVDSKINERIDGQLRFYDGYSHKISFSLPKNIKSEFQKITKISTEKDPIYVPA
>Mature_301_residues
MFGWHWLLEWQTPYEFHGHLIEKVLAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDEHIYHELLVHPLLLSLTKPPK
SVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKKYLYEWHQGAFEDKRTNLVITDGLKFIKETKEKYDAIILDL
TDPIKNSTSYMLYTKEFYEKLREILNERGGIVTQATSPSFSLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGV
RPDLLSEDEVDSKINERIDGQLRFYDGYSHKISFSLPKNIKSEFQKITKISTEKDPIYVPA

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=270, Percent_Identity=27.7777777777778, Blast_Score=131, Evalue=9e-31,
Organism=Escherichia coli, GI1786312, Length=285, Percent_Identity=34.3859649122807, Blast_Score=151, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI25154106, Length=267, Percent_Identity=35.2059925093633, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI212645616, Length=233, Percent_Identity=37.3390557939914, Blast_Score=130, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6323175, Length=265, Percent_Identity=32.0754716981132, Blast_Score=144, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6325326, Length=265, Percent_Identity=34.3396226415094, Blast_Score=142, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24645443, Length=289, Percent_Identity=32.5259515570934, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI21355941, Length=221, Percent_Identity=33.0316742081448, Blast_Score=110, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 34854; Mature: 34854

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGWHWLLEWQTPYEFHGHLIEKVLAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDE
CCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEHEEHHHCCCCEEECCCHHHHHHHH
HIYHELLVHPLLLSLTKPPKSVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKK
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCHHEEEECHHHHHHHHHH
YLYEWHQGAFEDKRTNLVITDGLKFIKETKEKYDAIILDLTDPIKNSTSYMLYTKEFYEK
HHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHCEEEEEECCCCCCCCEEEEEHHHHHHH
LREILNERGGIVTQATSPSFSLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGV
HHHHHHHCCCEEEEECCCCEEEEEEEEEHHHHHHHHHHHCCCEEEEEECCCHHHHHCCCC
RPDLLSEDEVDSKINERIDGQLRFYDGYSHKISFSLPKNIKSEFQKITKISTEKDPIYVP
CCCCCCCCHHHHHHHHCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEC
A
C
>Mature Secondary Structure
MFGWHWLLEWQTPYEFHGHLIEKVLAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDE
CCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEHEEHHHCCCCEEECCCHHHHHHHH
HIYHELLVHPLLLSLTKPPKSVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKK
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCHHEEEECHHHHHHHHHH
YLYEWHQGAFEDKRTNLVITDGLKFIKETKEKYDAIILDLTDPIKNSTSYMLYTKEFYEK
HHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHCEEEEEECCCCCCCCEEEEEHHHHHHH
LREILNERGGIVTQATSPSFSLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGV
HHHHHHHCCCEEEEECCCCEEEEEEEEEHHHHHHHHHHHCCCEEEEEECCCHHHHHCCCC
RPDLLSEDEVDSKINERIDGQLRFYDGYSHKISFSLPKNIKSEFQKITKISTEKDPIYVP
CCCCCCCCHHHHHHHHCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA