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The map label for this gene is NuoB [H]

Identifier: 15897574

GI number: 15897574

Start: 574064

End: 574576

Strand: Direct

Name: NuoB [H]

Synonym: SSO0665

Alternate gene names: 15897574

Gene position: 574064-574576 (Clockwise)

Preceding gene: 15897571

Following gene: 15897578

Centisome position: 19.19

GC content: 39.77

Gene sequence:

>513_bases
ATGACAGAACAGACTATTTTAATGGGTAATTTAAATGAAGTAGCTAAAAAGGCGGCTCAATGGCTAATTAATAGGAAACC
CATCAAATCGATAATTGATTGGGGAATTTCATTTTCTTTATGGCCACCTCACTTTACTACATCTTGTTGTGGTACGGAAT
TTGGAGCTTTCGCAGCTGCAAGATTTGATGCAGAGAGATATGGGATGTTGCCATTCTCCTCTGCTAGACAATCAAATATA
CTTATACTGGAAGGTACGCTAAGTAGAAAAATGGGTAGAGCAGCGAGAATTGTTTATGACCAGATGCCAGAACCAAAATA
CGTTATCGCCCTTGGAGCGTGTATACTCGAAGGAGGAATCTTTTGGAATTCTTATAATACCGTGCTTCCCTCAGATATTG
GAATACCAGTTGATTTATACGTTCCGGGATGCCCAATAAGGCCAGAGGCTGTAGCTAGAGCAGTATTAATGTTACAGAAG
AAGATAAGAACTCAAGGAGCCCTTAAGACATAA

Upstream 100 bases:

>100_bases
AATATACTTGTCGATGATGAGTCAAGAAAAATGATTTGATGATTTTTGAGGTGATCTCTGAATAAAGTTATTTAAAGGAG
TAGGAAAAGAGTGTATAGGA

Downstream 100 bases:

>100_bases
ATGTTATTTACATTAGTGTGAGTATATCCTGTTTTTATAACAGCTTTATGGCTTTCTAGCAATCCATAGCTATTATGAGT
CTCCAATGCATTATTTATCT

Product: NADH dehydrogenase subunit B (NuoB)

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit K 2; NDH-1 subunit K 2; NDH-K 2 [H]

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MTEQTILMGNLNEVAKKAAQWLINRKPIKSIIDWGISFSLWPPHFTTSCCGTEFGAFAAARFDAERYGMLPFSSARQSNI
LILEGTLSRKMGRAARIVYDQMPEPKYVIALGACILEGGIFWNSYNTVLPSDIGIPVDLYVPGCPIRPEAVARAVLMLQK
KIRTQGALKT

Sequences:

>Translated_170_residues
MTEQTILMGNLNEVAKKAAQWLINRKPIKSIIDWGISFSLWPPHFTTSCCGTEFGAFAAARFDAERYGMLPFSSARQSNI
LILEGTLSRKMGRAARIVYDQMPEPKYVIALGACILEGGIFWNSYNTVLPSDIGIPVDLYVPGCPIRPEAVARAVLMLQK
KIRTQGALKT
>Mature_169_residues
TEQTILMGNLNEVAKKAAQWLINRKPIKSIIDWGISFSLWPPHFTTSCCGTEFGAFAAARFDAERYGMLPFSSARQSNIL
ILEGTLSRKMGRAARIVYDQMPEPKYVIALGACILEGGIFWNSYNTVLPSDIGIPVDLYVPGCPIRPEAVARAVLMLQKK
IRTQGALKT

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=142, Percent_Identity=42.9577464788732, Blast_Score=135, Evalue=2e-32,
Organism=Escherichia coli, GI1788624, Length=136, Percent_Identity=40.4411764705882, Blast_Score=110, Evalue=3e-26,
Organism=Escherichia coli, GI1789074, Length=124, Percent_Identity=38.7096774193548, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788834, Length=121, Percent_Identity=38.8429752066116, Blast_Score=82, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17509685, Length=155, Percent_Identity=40.6451612903226, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI18859983, Length=151, Percent_Identity=44.3708609271523, Blast_Score=140, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24642371, Length=151, Percent_Identity=44.3708609271523, Blast_Score=140, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24651058, Length=154, Percent_Identity=42.8571428571429, Blast_Score=136, Evalue=6e-33,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 18823; Mature: 18692

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQTILMGNLNEVAKKAAQWLINRKPIKSIIDWGISFSLWPPHFTTSCCGTEFGAFAAA
CCCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCHHHCCHHHHHHHH
RFDAERYGMLPFSSARQSNILILEGTLSRKMGRAARIVYDQMPEPKYVIALGACILEGGI
HHCHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHCCE
FWNSYNTVLPSDIGIPVDLYVPGCPIRPEAVARAVLMLQKKIRTQGALKT
EECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TEQTILMGNLNEVAKKAAQWLINRKPIKSIIDWGISFSLWPPHFTTSCCGTEFGAFAAA
CCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCHHHCCHHHHHHHH
RFDAERYGMLPFSSARQSNILILEGTLSRKMGRAARIVYDQMPEPKYVIALGACILEGGI
HHCHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHCCE
FWNSYNTVLPSDIGIPVDLYVPGCPIRPEAVARAVLMLQKKIRTQGALKT
EECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA