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The map label for this gene is rimK-2 [H]

Identifier: 15897555

GI number: 15897555

Start: 559125

End: 559973

Strand: Direct

Name: rimK-2 [H]

Synonym: SSO0645

Alternate gene names: 15897555

Gene position: 559125-559973 (Clockwise)

Preceding gene: 15897554

Following gene: 15897557

Centisome position: 18.69

GC content: 35.69

Gene sequence:

>849_bases
TTGAAGATAGCCTTAGTAGTAGATATAATTAGACAAGAGGAGAAGTTAATAATAAAGGCATTAAATGAAAGACAAATAGA
CTATGACGTAATTAACGTAGGCCAAGAACCTATCCCATTCAATAAAGCACTAAGCAAGTACGATGTTGGTATAATCAGAC
CAGTAAGTATGTATAGGGCTCTTTACTCATCTGCGATACTAGAGGCCGCCGGTGTACATACGATAAATTCAACAGACGCT
ATTGGCACTTGCGGAGATAAGATACTTACATATTCTAAACTATTTAAACATGGAATTCCAATACCGGACTCAATAATAGC
AGCATCTCCAGATGCAGCTATGAAAGCATATGAGCAAATGGGATTCCCACTGATAGATAAACCGCCTATCGGAAGTTGGG
GAAGGATGGTATCTTTGATAAGAGATGTATATGAGGGAAAGACAATTATAGAGCATAGAGAAATGCTTAATAATTCAGCG
CTAAAAGTACATATTATTCAAGAATATATCAAATACAAAGAAAGAGATATAAGATGTATAGTAATAGGTGATGAGCTATT
GGGATGCTATGCAAGAAATATTCCACCTAATGAGTGGAGAGCAAATGTAGCATTGGGAGGAAATCCTACACCTATTCAGT
TAGATGATAACATAAGAGAATTAGCTGTAAAGTCTGTAAAGTTAGTAAAAGGCGAATTCGTATCTATAGACATATTAGAG
CACAAATCAAAAGGATATGTTATAAATGAGCTAAATGATGTTCCAGAATTTAAAGGATTTATGCTAGCTACTGGTATCAA
TGTTGCCGAAAAGCTAGTAGATCACATCGTTAAGACCTACCGAGCTTAG

Upstream 100 bases:

>100_bases
AATTCTTAACGGTAGATTAGGTTATGAGGTGAGTAAGGCTTTCCTAAATTATGACTCACTTACTTTTTTTGAAATATCTG
AGTATGGAGGAGGAATATGA

Downstream 100 bases:

>100_bases
CCAGAAAAATATCGTGATTAGTCTCCTCTAATAGTCTTCTTATGGTATCAATCTTTTGTCTTAGTCCAGGGACCAAGGAT
TTAGGGTACTCGAAACTCCA

Product: ribosomal protein S6 modification protein (rimK-2)

Products: NA

Alternate protein names: AAA--lysW ligase lysX [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MKIALVVDIIRQEEKLIIKALNERQIDYDVINVGQEPIPFNKALSKYDVGIIRPVSMYRALYSSAILEAAGVHTINSTDA
IGTCGDKILTYSKLFKHGIPIPDSIIAASPDAAMKAYEQMGFPLIDKPPIGSWGRMVSLIRDVYEGKTIIEHREMLNNSA
LKVHIIQEYIKYKERDIRCIVIGDELLGCYARNIPPNEWRANVALGGNPTPIQLDDNIRELAVKSVKLVKGEFVSIDILE
HKSKGYVINELNDVPEFKGFMLATGINVAEKLVDHIVKTYRA

Sequences:

>Translated_282_residues
MKIALVVDIIRQEEKLIIKALNERQIDYDVINVGQEPIPFNKALSKYDVGIIRPVSMYRALYSSAILEAAGVHTINSTDA
IGTCGDKILTYSKLFKHGIPIPDSIIAASPDAAMKAYEQMGFPLIDKPPIGSWGRMVSLIRDVYEGKTIIEHREMLNNSA
LKVHIIQEYIKYKERDIRCIVIGDELLGCYARNIPPNEWRANVALGGNPTPIQLDDNIRELAVKSVKLVKGEFVSIDILE
HKSKGYVINELNDVPEFKGFMLATGINVAEKLVDHIVKTYRA
>Mature_282_residues
MKIALVVDIIRQEEKLIIKALNERQIDYDVINVGQEPIPFNKALSKYDVGIIRPVSMYRALYSSAILEAAGVHTINSTDA
IGTCGDKILTYSKLFKHGIPIPDSIIAASPDAAMKAYEQMGFPLIDKPPIGSWGRMVSLIRDVYEGKTIIEHREMLNNSA
LKVHIIQEYIKYKERDIRCIVIGDELLGCYARNIPPNEWRANVALGGNPTPIQLDDNIRELAVKSVKLVKGEFVSIDILE
HKSKGYVINELNDVPEFKGFMLATGINVAEKLVDHIVKTYRA

Specific function: Catalyzes the ATP-dependent binding between the amino group of alpha-aminoadipate (AAA) and the gamma-carboxyl group of the C-terminal glutamate residue of lysW [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=216, Percent_Identity=25.462962962963, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787076, Length=221, Percent_Identity=30.316742081448, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011870
- InterPro:   IPR016185
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 31574; Mature: 31574

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIALVVDIIRQEEKLIIKALNERQIDYDVINVGQEPIPFNKALSKYDVGIIRPVSMYRA
CEEEEEHHHHHHHHHHHHHHHHCCCCCHHHEECCCCCCCHHHHHHHCCCCEECHHHHHHH
LYSSAILEAAGVHTINSTDAIGTCGDKILTYSKLFKHGIPIPDSIIAASPDAAMKAYEQM
HHHHHHHHHHCCEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHEECCCHHHHHHHHHC
GFPLIDKPPIGSWGRMVSLIRDVYEGKTIIEHREMLNNSALKVHIIQEYIKYKERDIRCI
CCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCEEE
VIGDELLGCYARNIPPNEWRANVALGGNPTPIQLDDNIRELAVKSVKLVKGEFVSIDILE
EECHHHHHHHHCCCCCCCCEEEEEECCCCCCEEECCCHHHHHHHHHHHHCCCEEEEEEEE
HKSKGYVINELNDVPEFKGFMLATGINVAEKLVDHIVKTYRA
CCCCCCEECCCCCCCHHCCEEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIALVVDIIRQEEKLIIKALNERQIDYDVINVGQEPIPFNKALSKYDVGIIRPVSMYRA
CEEEEEHHHHHHHHHHHHHHHHCCCCCHHHEECCCCCCCHHHHHHHCCCCEECHHHHHHH
LYSSAILEAAGVHTINSTDAIGTCGDKILTYSKLFKHGIPIPDSIIAASPDAAMKAYEQM
HHHHHHHHHHCCEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHEECCCHHHHHHHHHC
GFPLIDKPPIGSWGRMVSLIRDVYEGKTIIEHREMLNNSALKVHIIQEYIKYKERDIRCI
CCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCEEE
VIGDELLGCYARNIPPNEWRANVALGGNPTPIQLDDNIRELAVKSVKLVKGEFVSIDILE
EECHHHHHHHHCCCCCCCCEEEEEECCCCCCEEECCCHHHHHHHHHHHHCCCEEEEEEEE
HKSKGYVINELNDVPEFKGFMLATGINVAEKLVDHIVKTYRA
CCCCCCEECCCCCCCHHCCEEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]