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The map label for this gene is proS [H]

Identifier: 15897489

GI number: 15897489

Start: 500806

End: 502251

Strand: Direct

Name: proS [H]

Synonym: SSO0569

Alternate gene names: 15897489

Gene position: 500806-502251 (Clockwise)

Preceding gene: 15897488

Following gene: 15897490

Centisome position: 16.74

GC content: 35.96

Gene sequence:

>1446_bases
GTGCAGATAACTAGAGAGAAGTGGTCAAAAAACTTTAGTGAATGGTTTGATTGGGTATTAAGAGAAGGAGAATTTTACGA
TTATGGGAGATATCCAATAAAAGGTATGGGAGTATGGATGCCATATGGATTTAAGCTAAGACAAAATATAATAGGAATAA
TTAGAAATTTTCTAGACTCTACTGGACATGAGGAAGTATTGTTTCCACTTCTAATCCCAGAAGATTTGCTAAGGAGAGAA
AGCACGCATATTAAAGGATTTGAAGAGGAGGTATTCTGGGTAACTAAGGGAGGAAGCACGGACTTAGACGTTAAACTTGC
GCTAAGACCAACTAGTGAAGTCGCAATAACCACAATGGAAAACTTATGGTTGAAAAGCTACAAGCAATTACCTAAAAAGT
ACTATCAAATTGTAAGCGTCTTTAGATATGAAACTAAGGCTACTAGGCCAATGATAAGATTAAGAGAAATTACAACTTTT
AAAGAAGCCCATACGGTTCATGAAACTTACGATGATGCACAGAAACAAGTAGAAGAGGCAATAGAAATTTATAAGAAGAT
TTTTGACACTCTCACAATCCCATACGTCCTTTCTGAAAGACCAGAGTGGGATAGATTTGCCGGAGCTTTGCACACTTATG
CTTTCGATACTATTATGCCGGATGGTAGAGCAATGCAAATAGGTACAGTGCATCACTTAGGTCAAAACTTTAGTAAGGCA
TTAGATTTTAAGATACAGAAAAAAGATGGTAGTTTAGACTATCCACACCAAACAAGTTATGGTATTTCAGACAGAGCAGT
AGCTTCAGTAATTGCAATACATGGAGATGATCACGGACCGGTTTTACCTCCTTCTGTAGCTCCCATTAAAGTAGTAGTAA
TACCCATTCCTGCAAAAAATGAAGAGGAAACTCAACAAGTAATGAAATACTCAATTGAGATCTGTGAGATGTTTAACAAG
AACAATATAACTTGTTTGACTGATCAAGATACTGAGAAAACACCTGGGGAGAAGTTCTATATTTGGGAAATTAAGGGAGT
TCCAATTAGACTAGAAATCGGCCCAAGGGAGCTTATTTCTGGCACAGTATTTATAAAGAGAAGAGACAATTTTAAGTCTT
ATACTGTAAGGAAAGAAGAAGCCATCAATAAGATTAAAGAACTTCTAAATGAAATCCAGGAAGACTTAAGAAGAAAGGCT
TGGGAGGATCTTAAATCTAGAATAGAGTATTCGAATGATATTGAAAAAGCTAAGAAGATATTAGGGAATAACTCAGGTAT
TGTAGAAGTCCCTTGGTGTGGAAGTAACGAGTGTGGCTTAAAAATTGAGGAACTTACCAATGCGAGAGTACTAGGTTATC
CAATAGAGAAGAGAGAAGTTAATGATAAGTGCGTAATTTGTAAAATGAACGCAAAAACAGTTCTAAGGATTGCAAAAACT
TATTAG

Upstream 100 bases:

>100_bases
GAATAGCAGTATACGGAATCTTGTTCGCTGTACTAATGTTGTTCGGTAAGTTCTAAAATAAAGGTTAAAGTTTTTTATCT
CCTTATGTTTTTTCCTTTTT

Downstream 100 bases:

>100_bases
AGATTAAATAGTGTGAATTAAATAGGGTGTAAAATTGCCAAAGAAGAGAGAGAACAGAGGAAGAAGAAAAGGAGATAAAG
GACATGTTGGTTATATAAGT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MQITREKWSKNFSEWFDWVLREGEFYDYGRYPIKGMGVWMPYGFKLRQNIIGIIRNFLDSTGHEEVLFPLLIPEDLLRRE
STHIKGFEEEVFWVTKGGSTDLDVKLALRPTSEVAITTMENLWLKSYKQLPKKYYQIVSVFRYETKATRPMIRLREITTF
KEAHTVHETYDDAQKQVEEAIEIYKKIFDTLTIPYVLSERPEWDRFAGALHTYAFDTIMPDGRAMQIGTVHHLGQNFSKA
LDFKIQKKDGSLDYPHQTSYGISDRAVASVIAIHGDDHGPVLPPSVAPIKVVVIPIPAKNEEETQQVMKYSIEICEMFNK
NNITCLTDQDTEKTPGEKFYIWEIKGVPIRLEIGPRELISGTVFIKRRDNFKSYTVRKEEAINKIKELLNEIQEDLRRKA
WEDLKSRIEYSNDIEKAKKILGNNSGIVEVPWCGSNECGLKIEELTNARVLGYPIEKREVNDKCVICKMNAKTVLRIAKT
Y

Sequences:

>Translated_481_residues
MQITREKWSKNFSEWFDWVLREGEFYDYGRYPIKGMGVWMPYGFKLRQNIIGIIRNFLDSTGHEEVLFPLLIPEDLLRRE
STHIKGFEEEVFWVTKGGSTDLDVKLALRPTSEVAITTMENLWLKSYKQLPKKYYQIVSVFRYETKATRPMIRLREITTF
KEAHTVHETYDDAQKQVEEAIEIYKKIFDTLTIPYVLSERPEWDRFAGALHTYAFDTIMPDGRAMQIGTVHHLGQNFSKA
LDFKIQKKDGSLDYPHQTSYGISDRAVASVIAIHGDDHGPVLPPSVAPIKVVVIPIPAKNEEETQQVMKYSIEICEMFNK
NNITCLTDQDTEKTPGEKFYIWEIKGVPIRLEIGPRELISGTVFIKRRDNFKSYTVRKEEAINKIKELLNEIQEDLRRKA
WEDLKSRIEYSNDIEKAKKILGNNSGIVEVPWCGSNECGLKIEELTNARVLGYPIEKREVNDKCVICKMNAKTVLRIAKT
Y
>Mature_481_residues
MQITREKWSKNFSEWFDWVLREGEFYDYGRYPIKGMGVWMPYGFKLRQNIIGIIRNFLDSTGHEEVLFPLLIPEDLLRRE
STHIKGFEEEVFWVTKGGSTDLDVKLALRPTSEVAITTMENLWLKSYKQLPKKYYQIVSVFRYETKATRPMIRLREITTF
KEAHTVHETYDDAQKQVEEAIEIYKKIFDTLTIPYVLSERPEWDRFAGALHTYAFDTIMPDGRAMQIGTVHHLGQNFSKA
LDFKIQKKDGSLDYPHQTSYGISDRAVASVIAIHGDDHGPVLPPSVAPIKVVVIPIPAKNEEETQQVMKYSIEICEMFNK
NNITCLTDQDTEKTPGEKFYIWEIKGVPIRLEIGPRELISGTVFIKRRDNFKSYTVRKEEAINKIKELLNEIQEDLRRKA
WEDLKSRIEYSNDIEKAKKILGNNSGIVEVPWCGSNECGLKIEELTNARVLGYPIEKREVNDKCVICKMNAKTVLRIAKT
Y

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=501, Percent_Identity=32.9341317365269, Blast_Score=265, Evalue=9e-71,
Organism=Escherichia coli, GI1786392, Length=162, Percent_Identity=29.6296296296296, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71991342, Length=489, Percent_Identity=31.9018404907975, Blast_Score=255, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI71991346, Length=352, Percent_Identity=34.6590909090909, Blast_Score=221, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6321808, Length=500, Percent_Identity=31.6, Blast_Score=264, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24649468, Length=448, Percent_Identity=33.0357142857143, Blast_Score=236, Evalue=4e-62,
Organism=Drosophila melanogaster, GI24649466, Length=448, Percent_Identity=33.0357142857143, Blast_Score=235, Evalue=5e-62,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 55853; Mature: 55853

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITREKWSKNFSEWFDWVLREGEFYDYGRYPIKGMGVWMPYGFKLRQNIIGIIRNFLDS
CCCCHHHHCCCHHHHHHHHHHCCCEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHC
TGHEEVLFPLLIPEDLLRRESTHIKGFEEEVFWVTKGGSTDLDVKLALRPTSEVAITTME
CCCCCEEEEEECCHHHHHCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEHHHH
NLWLKSYKQLPKKYYQIVSVFRYETKATRPMIRLREITTFKEAHTVHETYDDAQKQVEEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEIYKKIFDTLTIPYVLSERPEWDRFAGALHTYAFDTIMPDGRAMQIGTVHHLGQNFSKA
HHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHH
LDFKIQKKDGSLDYPHQTSYGISDRAVASVIAIHGDDHGPVLPPSVAPIKVVVIPIPAKN
HCEEEEECCCCCCCCCCCCCCCCHHHHEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCC
EEETQQVMKYSIEICEMFNKNNITCLTDQDTEKTPGEKFYIWEIKGVPIRLEIGPRELIS
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEEECEEEEEEECHHHHHC
GTVFIKRRDNFKSYTVRKEEAINKIKELLNEIQEDLRRKAWEDLKSRIEYSNDIEKAKKI
CEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LGNNSGIVEVPWCGSNECGLKIEELTNARVLGYPIEKREVNDKCVICKMNAKTVLRIAKT
HCCCCCEEEECCCCCCCCCEEHHHCCCCEEEECCCHHCCCCCCEEEEEECHHHHHHHHHC
Y
C
>Mature Secondary Structure
MQITREKWSKNFSEWFDWVLREGEFYDYGRYPIKGMGVWMPYGFKLRQNIIGIIRNFLDS
CCCCHHHHCCCHHHHHHHHHHCCCEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHC
TGHEEVLFPLLIPEDLLRRESTHIKGFEEEVFWVTKGGSTDLDVKLALRPTSEVAITTME
CCCCCEEEEEECCHHHHHCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEHHHH
NLWLKSYKQLPKKYYQIVSVFRYETKATRPMIRLREITTFKEAHTVHETYDDAQKQVEEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEIYKKIFDTLTIPYVLSERPEWDRFAGALHTYAFDTIMPDGRAMQIGTVHHLGQNFSKA
HHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHH
LDFKIQKKDGSLDYPHQTSYGISDRAVASVIAIHGDDHGPVLPPSVAPIKVVVIPIPAKN
HCEEEEECCCCCCCCCCCCCCCCHHHHEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCC
EEETQQVMKYSIEICEMFNKNNITCLTDQDTEKTPGEKFYIWEIKGVPIRLEIGPRELIS
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEEECEEEEEEECHHHHHC
GTVFIKRRDNFKSYTVRKEEAINKIKELLNEIQEDLRRKAWEDLKSRIEYSNDIEKAKKI
CEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LGNNSGIVEVPWCGSNECGLKIEELTNARVLGYPIEKREVNDKCVICKMNAKTVLRIAKT
HCCCCCEEEECCCCCCCCCEEHHHCCCCEEEECCCHHCCCCCCEEEEEECHHHHHHHHHC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA