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The map label for this gene is metS [H]

Identifier: 15897480

GI number: 15897480

Start: 491695

End: 493416

Strand: Direct

Name: metS [H]

Synonym: SSO0558

Alternate gene names: 15897480

Gene position: 491695-493416 (Clockwise)

Preceding gene: 15897478

Following gene: 15897482

Centisome position: 16.43

GC content: 36.0

Gene sequence:

>1722_bases
ATGAAAGTGCTAGTTACAGCCGCCTGGCCATATGTAAATTCGGTACCACATTTAGGAAACTTAATAGGTTCAATATTATC
AGCAGATGTCTTCGCTAGATATGCAAGGCTAAGATATGGTAAAGAAAACGTATTATTTGTAAGTGGAAGTGACGAGCACG
GAACTCCAATAGAGATAGAGGCTATAAAGAGAAAGGTGAATCCCAAGGAGCTTACTGATCAAGCTCATGAATACGATAGG
CACCTCTTCCTCAATGTTTGGAAAATAAGTTTTGATAACTACACTAGAACTGAAAGTGAGATACATAAGAAGTTCGTAAG
AGAATTTTTACTGAAACTTACTAAATATATTAAAGTGTCTGAAGACGAGATACCATATTGTGAGAATGATAAACTATATC
TACCAGACAGGTTTGTTAAAGGGACATGCCCATATTGTGGCTTTGAAGACGCTAGAGGTGATCAATGTGACAATTGTGGG
AAATTACTAACCCCAAGTTTACTTGTAAATCCGAAATGTAGTATCTGCGGAAAGACACCTGTATTTAAGAAAACCAAACA
CTGGTTTTTTGATTTATCAGAGTTTAATGATAAGATAAGAGGTTGGATTAGCAGCTCTAATGAAATGCCAGATAATGTTA
AGTCTGTCGCATTGAGCTGGGTTGGGGAGGGGCTGAAACCTAGAAGTATCACAAGGGATAATAAATGGGGTATCCCAGCA
CCATTTGAAGGAGCTCAAGATAAATCAATATATGTGTGGTTTGAGGCTCTTTTAGGTTATATATCAGCTGTAATTGAATA
TTTTGAAAGGAAAGGTGACCAAGAGAAGTGGAAAGAATATTGGTTTGGTCCTAACATAAAAAGCTATTATTTCATAGGAA
AAGATAACATCCCATTTCATGCAGTAATTCTTCCAGCTATGCTCATGGCTTCAGAAGAGGAATATCACTTGCCAGATGTA
ATAGCGGCTACGGAATATTTACTATATGAAGGACAAAAGTTCAGCAAAAGTAGAAAGATAGGAGTATGGATTGATGAAGC
ACCAGAGCTAATGGATGTAGAATACTGGAGATTTGTGCTAATTAGATTAAGACCAGAAGAGAAAGATACTAACTTTACTT
GGAGGGAAACTGTCAGAATAGTGAATACTGAACTTAATGACGATATTGGTAATTATGTAAATAGGGTACTTAGTATGGTG
AATAGGTACTATAGCGGGATTGTACCAGAGTTTAAGATTGATATTCTCGATGATAACGATAGGAAAATTATCAGTCTAAT
AAATGAGACGCCGAAAGTTGTTGGAGATCTATTCGAGAAAGGTAAATTAAAGGCCGGAACGGAGGAAATGCTGAAATTCG
TTAGGGAATGTAACGCATATTTAAATATGAAGGCTCCTTGGGACCTATACAAGAGTGGAAAAGAGATTGAATTAAAGAAT
ACACTTTACATTGGAACCAATTCGGTAAAAACGATAGCGATTTTATTATATCCGCTAATGCCCTCACATGCTCAGAAAAT
ATATGAAATGTTGAATATGGGGAATATAGAAAATGAGAAATGGGATGTTGCCTCAACGCTATCAGTTAACCCTGGTCATA
AGATAGGGAAAGTTAATGTACTTTTCAAGAAACTTGAACCAGAATTTGAGTCTAAGATAAAGGATAAACTGGAAAAAATA
AGGAAAGATATAGAAAAAATAAGACCTACCTTACTCAAGTGA

Upstream 100 bases:

>100_bases
ACTCTTTATAAAAGGATACTTTTTAACGTCTAATGCCATGTCCTTACTTAAAGTATGTTTAGTTATGTTTAATAAAGACT
TGCTCTAAACACAAAATATA

Downstream 100 bases:

>100_bases
GACGTAAGCCATAACCGGCTCGAATTTTCTTCCTCTTCCTTCATAGAACTTACTGAACATCTCGTAGAAATGAAGCCTTA
AATCTTGTATAAATACTACT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MKVLVTAAWPYVNSVPHLGNLIGSILSADVFARYARLRYGKENVLFVSGSDEHGTPIEIEAIKRKVNPKELTDQAHEYDR
HLFLNVWKISFDNYTRTESEIHKKFVREFLLKLTKYIKVSEDEIPYCENDKLYLPDRFVKGTCPYCGFEDARGDQCDNCG
KLLTPSLLVNPKCSICGKTPVFKKTKHWFFDLSEFNDKIRGWISSSNEMPDNVKSVALSWVGEGLKPRSITRDNKWGIPA
PFEGAQDKSIYVWFEALLGYISAVIEYFERKGDQEKWKEYWFGPNIKSYYFIGKDNIPFHAVILPAMLMASEEEYHLPDV
IAATEYLLYEGQKFSKSRKIGVWIDEAPELMDVEYWRFVLIRLRPEEKDTNFTWRETVRIVNTELNDDIGNYVNRVLSMV
NRYYSGIVPEFKIDILDDNDRKIISLINETPKVVGDLFEKGKLKAGTEEMLKFVRECNAYLNMKAPWDLYKSGKEIELKN
TLYIGTNSVKTIAILLYPLMPSHAQKIYEMLNMGNIENEKWDVASTLSVNPGHKIGKVNVLFKKLEPEFESKIKDKLEKI
RKDIEKIRPTLLK

Sequences:

>Translated_573_residues
MKVLVTAAWPYVNSVPHLGNLIGSILSADVFARYARLRYGKENVLFVSGSDEHGTPIEIEAIKRKVNPKELTDQAHEYDR
HLFLNVWKISFDNYTRTESEIHKKFVREFLLKLTKYIKVSEDEIPYCENDKLYLPDRFVKGTCPYCGFEDARGDQCDNCG
KLLTPSLLVNPKCSICGKTPVFKKTKHWFFDLSEFNDKIRGWISSSNEMPDNVKSVALSWVGEGLKPRSITRDNKWGIPA
PFEGAQDKSIYVWFEALLGYISAVIEYFERKGDQEKWKEYWFGPNIKSYYFIGKDNIPFHAVILPAMLMASEEEYHLPDV
IAATEYLLYEGQKFSKSRKIGVWIDEAPELMDVEYWRFVLIRLRPEEKDTNFTWRETVRIVNTELNDDIGNYVNRVLSMV
NRYYSGIVPEFKIDILDDNDRKIISLINETPKVVGDLFEKGKLKAGTEEMLKFVRECNAYLNMKAPWDLYKSGKEIELKN
TLYIGTNSVKTIAILLYPLMPSHAQKIYEMLNMGNIENEKWDVASTLSVNPGHKIGKVNVLFKKLEPEFESKIKDKLEKI
RKDIEKIRPTLLK
>Mature_573_residues
MKVLVTAAWPYVNSVPHLGNLIGSILSADVFARYARLRYGKENVLFVSGSDEHGTPIEIEAIKRKVNPKELTDQAHEYDR
HLFLNVWKISFDNYTRTESEIHKKFVREFLLKLTKYIKVSEDEIPYCENDKLYLPDRFVKGTCPYCGFEDARGDQCDNCG
KLLTPSLLVNPKCSICGKTPVFKKTKHWFFDLSEFNDKIRGWISSSNEMPDNVKSVALSWVGEGLKPRSITRDNKWGIPA
PFEGAQDKSIYVWFEALLGYISAVIEYFERKGDQEKWKEYWFGPNIKSYYFIGKDNIPFHAVILPAMLMASEEEYHLPDV
IAATEYLLYEGQKFSKSRKIGVWIDEAPELMDVEYWRFVLIRLRPEEKDTNFTWRETVRIVNTELNDDIGNYVNRVLSMV
NRYYSGIVPEFKIDILDDNDRKIISLINETPKVVGDLFEKGKLKAGTEEMLKFVRECNAYLNMKAPWDLYKSGKEIELKN
TLYIGTNSVKTIAILLYPLMPSHAQKIYEMLNMGNIENEKWDVASTLSVNPGHKIGKVNVLFKKLEPEFESKIKDKLEKI
RKDIEKIRPTLLK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=569, Percent_Identity=36.0281195079086, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI24308436, Length=535, Percent_Identity=27.1028037383178, Blast_Score=166, Evalue=5e-41,
Organism=Escherichia coli, GI1788432, Length=559, Percent_Identity=29.5169946332737, Blast_Score=249, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI17540794, Length=565, Percent_Identity=37.1681415929204, Blast_Score=380, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI212645096, Length=561, Percent_Identity=26.3814616755793, Blast_Score=158, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI71992781, Length=383, Percent_Identity=26.8929503916449, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321704, Length=563, Percent_Identity=36.234458259325, Blast_Score=372, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6321610, Length=555, Percent_Identity=23.6036036036036, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24655311, Length=562, Percent_Identity=38.0782918149466, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI21358483, Length=543, Percent_Identity=24.8618784530387, Blast_Score=159, Evalue=7e-39,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 66517; Mature: 66517

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVTAAWPYVNSVPHLGNLIGSILSADVFARYARLRYGKENVLFVSGSDEHGTPIEIE
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEHH
AIKRKVNPKELTDQAHEYDRHLFLNVWKISFDNYTRTESEIHKKFVREFLLKLTKYIKVS
HHHHCCCHHHHHHHHHHHCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
EDEIPYCENDKLYLPDRFVKGTCPYCGFEDARGDQCDNCGKLLTPSLLVNPKCSICGKTP
CCCCCCCCCCCEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCC
VFKKTKHWFFDLSEFNDKIRGWISSSNEMPDNVKSVALSWVGEGLKPRSITRDNKWGIPA
CCCCCHHHEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PFEGAQDKSIYVWFEALLGYISAVIEYFERKGDQEKWKEYWFGPNIKSYYFIGKDNIPFH
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEECCCCCCCH
AVILPAMLMASEEEYHLPDVIAATEYLLYEGQKFSKSRKIGVWIDEAPELMDVEYWRFVL
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHCCCCCEEEEECCCCCHHHHHHEEEEE
IRLRPEEKDTNFTWRETVRIVNTELNDDIGNYVNRVLSMVNRYYSGIVPEFKIDILDDND
EEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
RKIISLINETPKVVGDLFEKGKLKAGTEEMLKFVRECNAYLNMKAPWDLYKSGKEIELKN
HHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEEEC
TLYIGTNSVKTIAILLYPLMPSHAQKIYEMLNMGNIENEKWDVASTLSVNPGHKIGKVNV
EEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCHHHH
LFKKLEPEFESKIKDKLEKIRKDIEKIRPTLLK
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVLVTAAWPYVNSVPHLGNLIGSILSADVFARYARLRYGKENVLFVSGSDEHGTPIEIE
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEHH
AIKRKVNPKELTDQAHEYDRHLFLNVWKISFDNYTRTESEIHKKFVREFLLKLTKYIKVS
HHHHCCCHHHHHHHHHHHCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
EDEIPYCENDKLYLPDRFVKGTCPYCGFEDARGDQCDNCGKLLTPSLLVNPKCSICGKTP
CCCCCCCCCCCEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCC
VFKKTKHWFFDLSEFNDKIRGWISSSNEMPDNVKSVALSWVGEGLKPRSITRDNKWGIPA
CCCCCHHHEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PFEGAQDKSIYVWFEALLGYISAVIEYFERKGDQEKWKEYWFGPNIKSYYFIGKDNIPFH
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEECCCCCCCH
AVILPAMLMASEEEYHLPDVIAATEYLLYEGQKFSKSRKIGVWIDEAPELMDVEYWRFVL
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHCCCCCEEEEECCCCCHHHHHHEEEEE
IRLRPEEKDTNFTWRETVRIVNTELNDDIGNYVNRVLSMVNRYYSGIVPEFKIDILDDND
EEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
RKIISLINETPKVVGDLFEKGKLKAGTEEMLKFVRECNAYLNMKAPWDLYKSGKEIELKN
HHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEEEC
TLYIGTNSVKTIAILLYPLMPSHAQKIYEMLNMGNIENEKWDVASTLSVNPGHKIGKVNV
EEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCHHHH
LFKKLEPEFESKIKDKLEKIRKDIEKIRPTLLK
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA