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The map label for this gene is pgk [H]

Identifier: 15897452

GI number: 15897452

Start: 456948

End: 458195

Strand: Direct

Name: pgk [H]

Synonym: SSO0527

Alternate gene names: 15897452

Gene position: 456948-458195 (Clockwise)

Preceding gene: 15897451

Following gene: 15897453

Centisome position: 15.27

GC content: 35.98

Gene sequence:

>1248_bases
GTGGGGATCTTTCTGATTAAAGTGGGAGACTTAACTATACCCACAATGGATGATATAGATATCCAATCAAAAAAAGTTCT
GTTACGAATAGACATAAATTCCCCCGTAGATGAGAACGGCAAAATAATTGATGATTCTAGAATAAAGGCACATATTGGAA
CAATTAAGGAATTGATTAATAAGGGCAATTCTGTTGTGTTAATTTCACATCAAGGTAGGCCTGGAGACAAGGATTTCACA
AGTTTAGAGGAACATGCTAAATTAATTTCAAAGTATTTAGATCGAGAAGTAATTTTCGTAGATGATGTAATAGGCCCATA
TGCAAGGGAAATGATTAAAAAATTAGAAAATAATGGGATTTTACTCTTAGATAATATAAGATTAATTTCAGAGGAGTTAA
TAGAGGCTCCACCACAACAACACGTAAAAAGCTTTTTAGTAAAGAAATTAGCTCCGCTTTTCGACATTTATATCAATGAT
GCGTTCGCTACTGCACACAGAAGTCAGCCTAGTATCGTAGGCTTTCCTTTAGCTTTACCATCAGCAGCTGGAAGAGTAAT
GGAAAGGGAAGTTTCAGCGTTAGCCAAGATTTTTAATGCCGAAGACACACCAAAGATATTTACGTTAGGTGGTGGTAAGG
TTCACGATACTATTAGGATAATTGAAAATCTAGTGAGAAAAAGGATAGCTGACAGAATACTTACTGGGGGTTTAGTTGCA
GAATTATTTTCTGTCGCTAAAGGAATGAATTTAAATCCAAAGAATATGGAAATTCTGGAAAAATTAGGTATACTGAGTCT
AATTCCTAGAGCTAGAAAACTACTTCTAAGTGGAGCTCCTATCGAAATACCAGTTGACTATAAGGTAGAAATAAACGGAA
ACGTAATCGAGGAACCAGCTAGTAAGGTCACTGGCATTATAAAGGATATCGGATCCACTACTGCAGAAATATACTCATCA
TTTATAAAAGATGCTAAAGTAGTAGTTTTAAGGGGACCAATGGGAGTAATCGAAGATGAAAGATTTAAGAGTGGAAGTAA
AAGCGTCTTAAAAGCATCATTAGAGGGACAAGGTTACGTTATAATAGGAGGTGGACACATGATAAGTGCTTTAGATGAGG
ATATGAAGATAGATAGTAGTAAAGTTCATATCTCTACTGGTGGAGGAGCTCTCCTCTTATTCTTATCTGGAGAACGATTA
CCAGCATTAGAGGCATTATCAATGTCGGTGGTGAATAGCGGTGATTAA

Upstream 100 bases:

>100_bases
ATCAGCTGAAGACTAAAGCCTTGATACCCCTAATTTGGGATCGTTTTATTATCGATTCTCTGCGTTGCTAAAAGTTATTA
CCCTAAAATGCGGAATATAA

Downstream 100 bases:

>100_bases
TGTAGCTGTTAACGGTTATGGTACTATAGGGAAAAGAGTCGCTGATGCCATCATTAAACAACCAGACATGAAATTAGTAG
GGGTTGCGAAGACATCTCCA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MGIFLIKVGDLTIPTMDDIDIQSKKVLLRIDINSPVDENGKIIDDSRIKAHIGTIKELINKGNSVVLISHQGRPGDKDFT
SLEEHAKLISKYLDREVIFVDDVIGPYAREMIKKLENNGILLLDNIRLISEELIEAPPQQHVKSFLVKKLAPLFDIYIND
AFATAHRSQPSIVGFPLALPSAAGRVMEREVSALAKIFNAEDTPKIFTLGGGKVHDTIRIIENLVRKRIADRILTGGLVA
ELFSVAKGMNLNPKNMEILEKLGILSLIPRARKLLLSGAPIEIPVDYKVEINGNVIEEPASKVTGIIKDIGSTTAEIYSS
FIKDAKVVVLRGPMGVIEDERFKSGSKSVLKASLEGQGYVIIGGGHMISALDEDMKIDSSKVHISTGGGALLLFLSGERL
PALEALSMSVVNSGD

Sequences:

>Translated_415_residues
MGIFLIKVGDLTIPTMDDIDIQSKKVLLRIDINSPVDENGKIIDDSRIKAHIGTIKELINKGNSVVLISHQGRPGDKDFT
SLEEHAKLISKYLDREVIFVDDVIGPYAREMIKKLENNGILLLDNIRLISEELIEAPPQQHVKSFLVKKLAPLFDIYIND
AFATAHRSQPSIVGFPLALPSAAGRVMEREVSALAKIFNAEDTPKIFTLGGGKVHDTIRIIENLVRKRIADRILTGGLVA
ELFSVAKGMNLNPKNMEILEKLGILSLIPRARKLLLSGAPIEIPVDYKVEINGNVIEEPASKVTGIIKDIGSTTAEIYSS
FIKDAKVVVLRGPMGVIEDERFKSGSKSVLKASLEGQGYVIIGGGHMISALDEDMKIDSSKVHISTGGGALLLFLSGERL
PALEALSMSVVNSGD
>Mature_414_residues
GIFLIKVGDLTIPTMDDIDIQSKKVLLRIDINSPVDENGKIIDDSRIKAHIGTIKELINKGNSVVLISHQGRPGDKDFTS
LEEHAKLISKYLDREVIFVDDVIGPYAREMIKKLENNGILLLDNIRLISEELIEAPPQQHVKSFLVKKLAPLFDIYINDA
FATAHRSQPSIVGFPLALPSAAGRVMEREVSALAKIFNAEDTPKIFTLGGGKVHDTIRIIENLVRKRIADRILTGGLVAE
LFSVAKGMNLNPKNMEILEKLGILSLIPRARKLLLSGAPIEIPVDYKVEINGNVIEEPASKVTGIIKDIGSTTAEIYSSF
IKDAKVVVLRGPMGVIEDERFKSGSKSVLKASLEGQGYVIIGGGHMISALDEDMKIDSSKVHISTGGGALLLFLSGERLP
ALEALSMSVVNSGD

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=426, Percent_Identity=33.5680751173709, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI31543397, Length=422, Percent_Identity=31.2796208530806, Blast_Score=175, Evalue=7e-44,
Organism=Escherichia coli, GI1789294, Length=408, Percent_Identity=30.3921568627451, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17508823, Length=417, Percent_Identity=33.0935251798561, Blast_Score=182, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6319857, Length=415, Percent_Identity=33.2530120481928, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI17136394, Length=424, Percent_Identity=32.311320754717, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24581203, Length=421, Percent_Identity=31.5914489311164, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 45167; Mature: 45036

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIFLIKVGDLTIPTMDDIDIQSKKVLLRIDINSPVDENGKIIDDSRIKAHIGTIKELIN
CCEEEEEECCEECCCCCCCCCCCCEEEEEEECCCCCCCCCCEECCCHHHHHHHHHHHHHH
KGNSVVLISHQGRPGDKDFTSLEEHAKLISKYLDREVIFVDDVIGPYAREMIKKLENNGI
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHCCHHHHHHHHHHCCCCE
LLLDNIRLISEELIEAPPQQHVKSFLVKKLAPLFDIYINDAFATAHRSQPSIVGFPLALP
EEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEECEECC
SAAGRVMEREVSALAKIFNAEDTPKIFTLGGGKVHDTIRIIENLVRKRIADRILTGGLVA
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
ELFSVAKGMNLNPKNMEILEKLGILSLIPRARKLLLSGAPIEIPVDYKVEINGNVIEEPA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEECCCEECCHH
SKVTGIIKDIGSTTAEIYSSFIKDAKVVVLRGPMGVIEDERFKSGSKSVLKASLEGQGYV
HHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHCCCCHHHHHHCCCCCEEE
IIGGGHMISALDEDMKIDSSKVHISTGGGALLLFLSGERLPALEALSMSVVNSGD
EEECCCEEHHHCCCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
GIFLIKVGDLTIPTMDDIDIQSKKVLLRIDINSPVDENGKIIDDSRIKAHIGTIKELIN
CEEEEEECCEECCCCCCCCCCCCEEEEEEECCCCCCCCCCEECCCHHHHHHHHHHHHHH
KGNSVVLISHQGRPGDKDFTSLEEHAKLISKYLDREVIFVDDVIGPYAREMIKKLENNGI
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHCCHHHHHHHHHHCCCCE
LLLDNIRLISEELIEAPPQQHVKSFLVKKLAPLFDIYINDAFATAHRSQPSIVGFPLALP
EEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEECEECC
SAAGRVMEREVSALAKIFNAEDTPKIFTLGGGKVHDTIRIIENLVRKRIADRILTGGLVA
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
ELFSVAKGMNLNPKNMEILEKLGILSLIPRARKLLLSGAPIEIPVDYKVEINGNVIEEPA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEECCCEECCHH
SKVTGIIKDIGSTTAEIYSSFIKDAKVVVLRGPMGVIEDERFKSGSKSVLKASLEGQGYV
HHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHCCCCHHHHHHCCCCCEEE
IIGGGHMISALDEDMKIDSSKVHISTGGGALLLFLSGERLPALEALSMSVVNSGD
EEECCCEEHHHCCCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8521845; 10701121; 11427726 [H]