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The map label for this gene is alaS-like1

Identifier: 15897381

GI number: 15897381

Start: 389485

End: 390195

Strand: Reverse

Name: alaS-like1

Synonym: SSO0450

Alternate gene names: 15897381

Gene position: 390195-389485 (Counterclockwise)

Preceding gene: 15897384

Following gene: 15897380

Centisome position: 13.04

GC content: 36.71

Gene sequence:

>711_bases
ATGACAGAAGAACTCTATCTAAAGGATTCTTACATTAAAGAATTCGAGGGAAGAGTAGTTAGGATAGAAGGTAACTACGT
AATACTTGATAAGACAGCTTTTTATCCAGGTGGAGGAGGTTTAGATAACGATACTGGATTCCTAGTCAATGAGAAAGGGG
AGAGAATAAGCGTTACTGAGGTAAAAAGGGGAGAAAACGGAGAGATTTTACATAAGATAGATCAAAATGGTTCCCTTAAT
GTTAATGAAAAAGTTATAGGCACAATAGATTGGGACAGAAGATATAGAATGATGCGATTACATACCGCTTCGCATATAGT
CGCTGCATTGGCTTATAGAAAATTCGGAGCTTTGATAACCGGAGGGCACATTAGCCCAGAACAAGCTAAGGACGATTTTA
ATGTTGAAAATAAAGATACCCTTATTGAGTTAATAAATGAAGCTAACGAAATAATCAAAAAAGACATAGAGTTGAAGATC
TATTTTCTACCTAGGGAAGAAGCTCTAATGATACCAGCTATAGTGAAACTAGCTGGAAGAAATCCACCACAGATTCCCAT
ATGGCGAATTGTTGAGATTCCGGGTATAGATATCCAAGCTGATGGTGGACCTCACGTTAAGAATACCAAGGAAATAGGAG
AAATAGTATTACTTAAAGTTGAAAACAAAGGGAAAGGGAGAAAGAGAGTTTACTATACTGTTAAACCATAG

Upstream 100 bases:

>100_bases
AATTAAAGTGATATCGTTACCAATAAGGATAGACCCATTAACCTTAACTTACAAGCACAAGAATGGAATATTTATATTAC
AAGGTAACAAGTTAAACTAA

Downstream 100 bases:

>100_bases
GGAATTTATTTATTAGTTTGGGCAGATAACTATTTTTGTGTCATTACTAATCAGGCTCGCTAAGTTTGCTTTAGTTGGTG
GTTTAGGCACAATTGTAAAT

Product: hypothetical protein

Products: AMP; diphosphate; L-alanyl-tRNA(Ala)

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MTEELYLKDSYIKEFEGRVVRIEGNYVILDKTAFYPGGGGLDNDTGFLVNEKGERISVTEVKRGENGEILHKIDQNGSLN
VNEKVIGTIDWDRRYRMMRLHTASHIVAALAYRKFGALITGGHISPEQAKDDFNVENKDTLIELINEANEIIKKDIELKI
YFLPREEALMIPAIVKLAGRNPPQIPIWRIVEIPGIDIQADGGPHVKNTKEIGEIVLLKVENKGKGRKRVYYTVKP

Sequences:

>Translated_236_residues
MTEELYLKDSYIKEFEGRVVRIEGNYVILDKTAFYPGGGGLDNDTGFLVNEKGERISVTEVKRGENGEILHKIDQNGSLN
VNEKVIGTIDWDRRYRMMRLHTASHIVAALAYRKFGALITGGHISPEQAKDDFNVENKDTLIELINEANEIIKKDIELKI
YFLPREEALMIPAIVKLAGRNPPQIPIWRIVEIPGIDIQADGGPHVKNTKEIGEIVLLKVENKGKGRKRVYYTVKP
>Mature_235_residues
TEELYLKDSYIKEFEGRVVRIEGNYVILDKTAFYPGGGGLDNDTGFLVNEKGERISVTEVKRGENGEILHKIDQNGSLNV
NEKVIGTIDWDRRYRMMRLHTASHIVAALAYRKFGALITGGHISPEQAKDDFNVENKDTLIELINEANEIIKKDIELKIY
FLPREEALMIPAIVKLAGRNPPQIPIWRIVEIPGIDIQADGGPHVKNTKEIGEIVLLKVENKGKGRKRVYYTVKP

Specific function: Functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala)

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily

Homologues:

Organism=Homo sapiens, GI217416402, Length=215, Percent_Identity=25.5813953488372, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI13376886, Length=215, Percent_Identity=25.5813953488372, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324911, Length=225, Percent_Identity=28, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI18543305, Length=253, Percent_Identity=26.0869565217391, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALAXM_SULSO (Q97ZX2)

Other databases:

- EMBL:   AE006641
- PIR:   A90190
- RefSeq:   NP_341986.1
- ProteinModelPortal:   Q97ZX2
- SMR:   Q97ZX2
- GeneID:   1455586
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0450
- NMPDR:   fig|273057.1.peg.409
- HOGENOM:   HBG691293
- OMA:   HMRLHTA
- PhylomeDB:   Q97ZX2
- ProtClustDB:   CLSK2301220
- BioCyc:   SSOL273057:SSO0450-MONOMER
- GO:   GO:0005737
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947
- SMART:   SM00863

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: 6.1.1.7

Molecular weight: Translated: 26710; Mature: 26579

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEELYLKDSYIKEFEGRVVRIEGNYVILDKTAFYPGGGGLDNDTGFLVNEKGERISVTE
CCCEEEECHHHHHHHCCEEEEEECCEEEEECCEECCCCCCCCCCCCEEECCCCCEEEEEE
VKRGENGEILHKIDQNGSLNVNEKVIGTIDWDRRYRMMRLHTASHIVAALAYRKFGALIT
ECCCCCCCEEEEECCCCCCEECCEEEEEECHHHCEEEEEHHHHHHHHHHHHHHHHCCEEE
GGHISPEQAKDDFNVENKDTLIELINEANEIIKKDIELKIYFLPREEALMIPAIVKLAGR
CCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEHHHHHHHHCC
NPPQIPIWRIVEIPGIDIQADGGPHVKNTKEIGEIVLLKVENKGKGRKRVYYTVKP
CCCCCCEEEEEEECCEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECC
>Mature Secondary Structure 
TEELYLKDSYIKEFEGRVVRIEGNYVILDKTAFYPGGGGLDNDTGFLVNEKGERISVTE
CCEEEECHHHHHHHCCEEEEEECCEEEEECCEECCCCCCCCCCCCEEECCCCCEEEEEE
VKRGENGEILHKIDQNGSLNVNEKVIGTIDWDRRYRMMRLHTASHIVAALAYRKFGALIT
ECCCCCCCEEEEECCCCCCEECCEEEEEECHHHCEEEEEHHHHHHHHHHHHHHHHCCEEE
GGHISPEQAKDDFNVENKDTLIELINEANEIIKKDIELKIYFLPREEALMIPAIVKLAGR
CCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEHHHHHHHHCC
NPPQIPIWRIVEIPGIDIQADGGPHVKNTKEIGEIVLLKVENKGKGRKRVYYTVKP
CCCCCCEEEEEEECCEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-alanine; tRNA(Ala)

Specific reaction: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726