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The map label for this gene is cobB

Identifier: 15897338

GI number: 15897338

Start: 353640

End: 354944

Strand: Reverse

Name: cobB

Synonym: SSO0404

Alternate gene names: 15897338

Gene position: 354944-353640 (Counterclockwise)

Preceding gene: 15897343

Following gene: 15897336

Centisome position: 11.86

GC content: 33.72

Gene sequence:

>1305_bases
ATGAAAAGGATATTGTTATCATCAGATAGAAGTGGATCAGGTAAAACGCTTATAACCTCAGCAATAATGAGAGCACTATC
TAAAAAATATAAAGTCAGAGGTTTCAAAGCGGGACCAGATTTCATAGATCCAGGATATCACAAGATTGCCACAGGTTTTC
CTTCAATAAACTTGGACTTATGGATGATGGGAAAAAATAATGTAAAAAGAAGTCTAGCGAAATACGGAAAGGAATTCGAT
ATAGGAATAATTGAGGGAGTAATGGGCTTATATGATGGTGTTGACACGCTTTACAGCACTTATGAGCTAGCCAAGGTTAC
TAAGACCCCAATAATTTTAATAATTAACTGCTCCAACATAGGGAGCACTGTAGGTGCAATTGTAAAGGGGCTAAAATATT
ATAGAAGCGACGTAAGTATAAGGGGTGTAATCTTTAATAAAATAGCTTCTGAGACCCATTATAATTACTGTAGGAACGCA
GTAGAGGATGTTGAAGTTTTAGGCTATGTCCCATTTGATAAGAATTTAGAAATTAAGTCTAGACATTTAGGTTTAGTTAC
CGTCGAAGATAATAGAGAAGTACAAAACTTAATTAGATATGCCTCAGAGTTAGTAGAGAAATACGTTGATTTAGATAAGA
TCTATGAAATGGCTTCAGATGAAGATCTTGAAATCGATCTTCCGGAAGATAATGAGAGTAATGGGGTAAAAAGAAAAATG
GCAATAGCTTACGATCCAGCATTTAGTTTCTATTATCAAGAAAACTTGGATATATTGAAAAATAAATACGAATTGGAATT
CTTCAGCCCTCTAAATAACGAATATGTGGAGGATGCGGAAGCCATATATATCGGGGGAGGTTATCCAGAACTTCATCTAA
ATGAGTTAGAAAAGTCCACTAGAACGAAGAAATGGCTTAAAAATATGTCTTACGCGGGAGTAAAAATCTATGCTGAGTGC
GGTGGACTAATGTACTTATCTAAAAACCTAATAGACGAAAATAATAAAAACCATAGTATGACTGGTATTTTTGATATTGA
TATAAAAACTAAAGATAAATTAACAATTGGATATACAGAATTAGAGGCAGTTAAAGAGAACTTCATAGTTAATAAGAACA
ATGTAGTCAGGGGGCATGAATTTCATGTGTCTAAACCCATAAGCGTTAATGAAAAAGAATTTGTATTTAAAGTTAGAATA
GGGAAAGGTATTATTAATAAATTGGACGGCGTAAAAAGCAATAACACTGTTGCAAGTTACTCTCACTTACACTTTTCTAA
CTTTCAGCTGAGGATTGTTTTTTGA

Upstream 100 bases:

>100_bases
TCTTATATTCCTTTCAAAAGATTTAAGATATATCATACTTTCAATTTAACTAAAGATTCACAAGATTTAACTACCTTTTA
AATATATACTTCTAATAGCA

Downstream 100 bases:

>100_bases
GCTCTATTTTCTTTACCCTCTCAATTAATTCGGAAACTTTATCTATATCTTGTGAATACAGTCTCACCTGGAATGGGAAC
TTTGATGAAGACCTAATCTC

Product: cobyrinic acid A,C diamisynthase (CBIA)

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MKRILLSSDRSGSGKTLITSAIMRALSKKYKVRGFKAGPDFIDPGYHKIATGFPSINLDLWMMGKNNVKRSLAKYGKEFD
IGIIEGVMGLYDGVDTLYSTYELAKVTKTPIILIINCSNIGSTVGAIVKGLKYYRSDVSIRGVIFNKIASETHYNYCRNA
VEDVEVLGYVPFDKNLEIKSRHLGLVTVEDNREVQNLIRYASELVEKYVDLDKIYEMASDEDLEIDLPEDNESNGVKRKM
AIAYDPAFSFYYQENLDILKNKYELEFFSPLNNEYVEDAEAIYIGGGYPELHLNELEKSTRTKKWLKNMSYAGVKIYAEC
GGLMYLSKNLIDENNKNHSMTGIFDIDIKTKDKLTIGYTELEAVKENFIVNKNNVVRGHEFHVSKPISVNEKEFVFKVRI
GKGIINKLDGVKSNNTVASYSHLHFSNFQLRIVF

Sequences:

>Translated_434_residues
MKRILLSSDRSGSGKTLITSAIMRALSKKYKVRGFKAGPDFIDPGYHKIATGFPSINLDLWMMGKNNVKRSLAKYGKEFD
IGIIEGVMGLYDGVDTLYSTYELAKVTKTPIILIINCSNIGSTVGAIVKGLKYYRSDVSIRGVIFNKIASETHYNYCRNA
VEDVEVLGYVPFDKNLEIKSRHLGLVTVEDNREVQNLIRYASELVEKYVDLDKIYEMASDEDLEIDLPEDNESNGVKRKM
AIAYDPAFSFYYQENLDILKNKYELEFFSPLNNEYVEDAEAIYIGGGYPELHLNELEKSTRTKKWLKNMSYAGVKIYAEC
GGLMYLSKNLIDENNKNHSMTGIFDIDIKTKDKLTIGYTELEAVKENFIVNKNNVVRGHEFHVSKPISVNEKEFVFKVRI
GKGIINKLDGVKSNNTVASYSHLHFSNFQLRIVF
>Mature_434_residues
MKRILLSSDRSGSGKTLITSAIMRALSKKYKVRGFKAGPDFIDPGYHKIATGFPSINLDLWMMGKNNVKRSLAKYGKEFD
IGIIEGVMGLYDGVDTLYSTYELAKVTKTPIILIINCSNIGSTVGAIVKGLKYYRSDVSIRGVIFNKIASETHYNYCRNA
VEDVEVLGYVPFDKNLEIKSRHLGLVTVEDNREVQNLIRYASELVEKYVDLDKIYEMASDEDLEIDLPEDNESNGVKRKM
AIAYDPAFSFYYQENLDILKNKYELEFFSPLNNEYVEDAEAIYIGGGYPELHLNELEKSTRTKKWLKNMSYAGVKIYAEC
GGLMYLSKNLIDENNKNHSMTGIFDIDIKTKDKLTIGYTELEAVKENFIVNKNNVVRGHEFHVSKPISVNEKEFVFKVRI
GKGIINKLDGVKSNNTVASYSHLHFSNFQLRIVF

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBB_SULSO (Q980B1)

Other databases:

- EMBL:   AE006641
- PIR:   F90184
- RefSeq:   NP_341943.1
- ProteinModelPortal:   Q980B1
- GeneID:   1455543
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0404
- NMPDR:   fig|273057.1.peg.366
- HOGENOM:   HBG634910
- OMA:   VFKCGPD
- ProtClustDB:   CLSK883637
- BioCyc:   SSOL273057:SSO0404-MONOMER
- HAMAP:   MF_00027
- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00379

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.9

Molecular weight: Translated: 49346; Mature: 49346

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 320-320 ACT_SITE 422-422

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRILLSSDRSGSGKTLITSAIMRALSKKYKVRGFKAGPDFIDPGYHKIATGFPSINLDL
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHCCCCCCCEEE
WMMGKNNVKRSLAKYGKEFDIGIIEGVMGLYDGVDTLYSTYELAKVTKTPIILIINCSNI
EEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
GSTVGAIVKGLKYYRSDVSIRGVIFNKIASETHYNYCRNAVEDVEVLGYVPFDKNLEIKS
CHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEE
RHLGLVTVEDNREVQNLIRYASELVEKYVDLDKIYEMASDEDLEIDLPEDNESNGVKRKM
EEEEEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEEEECCCCCCCCCCEEEE
AIAYDPAFSFYYQENLDILKNKYELEFFSPLNNEYVEDAEAIYIGGGYPELHLNELEKST
EEEECCCEEEEECCCHHHHCCCEEEEEECCCCCCHHCCCCEEEEECCCCCCHHHHHHHHH
RTKKWLKNMSYAGVKIYAECGGLMYLSKNLIDENNKNHSMTGIFDIDIKTKDKLTIGYTE
HHHHHHHCCCCCCEEEEEECCCEEEEHHHHHCCCCCCCCEEEEEEEEECCCCCEEECHHH
LEAVKENFIVNKNNVVRGHEFHVSKPISVNEKEFVFKVRIGKGIINKLDGVKSNNTVASY
HHHHHHHCEECCCCEEECCEEEECCCCCCCCCEEEEEEEECCHHHHHHCCCCCCCCEEEE
SHLHFSNFQLRIVF
EEEEECCEEEEEEC
>Mature Secondary Structure
MKRILLSSDRSGSGKTLITSAIMRALSKKYKVRGFKAGPDFIDPGYHKIATGFPSINLDL
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHCCCCCCCEEE
WMMGKNNVKRSLAKYGKEFDIGIIEGVMGLYDGVDTLYSTYELAKVTKTPIILIINCSNI
EEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
GSTVGAIVKGLKYYRSDVSIRGVIFNKIASETHYNYCRNAVEDVEVLGYVPFDKNLEIKS
CHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEE
RHLGLVTVEDNREVQNLIRYASELVEKYVDLDKIYEMASDEDLEIDLPEDNESNGVKRKM
EEEEEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEEEECCCCCCCCCCEEEE
AIAYDPAFSFYYQENLDILKNKYELEFFSPLNNEYVEDAEAIYIGGGYPELHLNELEKST
EEEECCCEEEEECCCHHHHCCCEEEEEECCCCCCHHCCCCEEEEECCCCCCHHHHHHHHH
RTKKWLKNMSYAGVKIYAECGGLMYLSKNLIDENNKNHSMTGIFDIDIKTKDKLTIGYTE
HHHHHHHCCCCCCEEEEEECCCEEEEHHHHHCCCCCCCCEEEEEEEEECCCCCEEECHHH
LEAVKENFIVNKNNVVRGHEFHVSKPISVNEKEFVFKVRIGKGIINKLDGVKSNNTVASY
HHHHHHHCEECCCCEEECCEEEECCCCCCCCCEEEEEEEECCHHHHHHCCCCCCCCEEEE
SHLHFSNFQLRIVF
EEEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726