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The map label for this gene is glnA-1

Identifier: 15897301

GI number: 15897301

Start: 311070

End: 312485

Strand: Reverse

Name: glnA-1

Synonym: SSO0366

Alternate gene names: 15897301

Gene position: 312485-311070 (Counterclockwise)

Preceding gene: 15897302

Following gene: 15897293

Centisome position: 10.44

GC content: 38.56

Gene sequence:

>1416_bases
ATGCCAAGTACAGCAGAAGATGTACTCAAATTCCTAAAGGAAAATAACATAAAATGGGTAGATCTACAATTCACAGATGT
TCCCGGTAGGCTGCATCATATTACAATACCTGCTGACGAGTTTGATTTAGAATCCTTAAAGACGGGTTTCGGAAAATTAG
ACGGAAGCAGTATAAGAGGATTTACGAGCATTTATGAAAGTGATATGGTACTCTTACCAGTACCAGAGACTATGACACTA
ATTCCTTGGTCTCCAGGAGTAGCTAGAGTCTTATGCAAAGTTTTTTGGGGAGGGGGAAAGGGAAGATTTGAAAGAGATCC
TAGATTCATAGCCGAGGAAGCCGAGAAATACCAGACAGAACAAGGCTACACTTCCTACTATGGTCCAGAATTGGAATTCT
TCATGTTTGATAAGGTAAAATTAGACGTAAGTGCCCCACAGTCTGGAACTGGGTACAAAATTTATGCAAGGGAGGCTCCG
TGGAGCGATAGTGGAACCTTTGTAATAAGATTTAAAGAGGGATATTACCCTGCACCACCAGTTGATCAGCTAATGGATGT
AAGAGTAGAGATAGTCGATACTTTAGTTAAGTACTTTGGATACACCATAGAGGCTACACATCATGAAGTTGCAACTGCAG
GTCAAGGGGAAATAGATTTCAGATTCTCTACTCTTGTAGATACTGCAGACAAGGTCCAAACTTTGAAGTATGTAGCTAAA
AATATTGCCGCTAAACACGGATTAGTAGTAACTTTCATGCCAAAACCTATTTATGGTGATAATGGAACTGGTATGCACAC
GCACCTCAGTTTATGGACTAAAGATGGAAAGAAGAATTTAATGTATGATCCTAATGACGAATATGCAGAGATAAGCCAAT
TTGGAAGATATGTAATAGGAGGTCTTTTAACACACGCTAGGGCTTTATCTGCAATAGTCTCACCTACTGTAAACAGTTAT
AGAAGGTTAATACCAGGGTTTGAAGCTCCAGTTTACATTGCGTGGAGTAAGGGAAACAGAAGTGCCGTAATTAGAGTGCC
AGCATACTATAGAGGAATGGAAAAGGCAAAAAGAATAGAGTATAGACCTCCAGATCCATCCACTAATCCGTATCTAGCAT
TTGCTGCTTTACTAATGGCTGCATTAGATGGTGTAAATAAGAAAATAGATCCAGGAGATCCAGTGGATGAGAATATATAC
CATCTAACTCCAGAGAAGAGAAGACAATTGGGAATAAAAGAACTACCTAGATCATTAAATGAAGCTTTAGATGAGTTAGA
AAGTGATAAAGAGTTCTTGAAGCCAGTATTTAATTCGTCAATATTAGATACTTACATAGATTTAAAGAGAGATGAGGCAA
GAAGCTTGCAAGGTTATCCACACCCAATGGAGTTATACTATTATTTAGATTCCTAA

Upstream 100 bases:

>100_bases
GATTTTGAGAAATCTCATGTGAAATTCCACATAAGAATGTGAAAACTTTCTATGATAATATCTAGCAATCTTTTTAAACT
ATATATGAATAGCCACATAC

Downstream 100 bases:

>100_bases
ATTGGTATAAAAATACCAGTATTTTTTAATGAGTTTTTGAACTCCTTAAGGTCGAGGTAATAATCGAATTCATAATTTTT
TGATGCTTCTTTTAGAATAT

Product: glutamine synthetase (glutamate ammonia ligase) (GS). (glnA-1)

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MPSTAEDVLKFLKENNIKWVDLQFTDVPGRLHHITIPADEFDLESLKTGFGKLDGSSIRGFTSIYESDMVLLPVPETMTL
IPWSPGVARVLCKVFWGGGKGRFERDPRFIAEEAEKYQTEQGYTSYYGPELEFFMFDKVKLDVSAPQSGTGYKIYAREAP
WSDSGTFVIRFKEGYYPAPPVDQLMDVRVEIVDTLVKYFGYTIEATHHEVATAGQGEIDFRFSTLVDTADKVQTLKYVAK
NIAAKHGLVVTFMPKPIYGDNGTGMHTHLSLWTKDGKKNLMYDPNDEYAEISQFGRYVIGGLLTHARALSAIVSPTVNSY
RRLIPGFEAPVYIAWSKGNRSAVIRVPAYYRGMEKAKRIEYRPPDPSTNPYLAFAALLMAALDGVNKKIDPGDPVDENIY
HLTPEKRRQLGIKELPRSLNEALDELESDKEFLKPVFNSSILDTYIDLKRDEARSLQGYPHPMELYYYLDS

Sequences:

>Translated_471_residues
MPSTAEDVLKFLKENNIKWVDLQFTDVPGRLHHITIPADEFDLESLKTGFGKLDGSSIRGFTSIYESDMVLLPVPETMTL
IPWSPGVARVLCKVFWGGGKGRFERDPRFIAEEAEKYQTEQGYTSYYGPELEFFMFDKVKLDVSAPQSGTGYKIYAREAP
WSDSGTFVIRFKEGYYPAPPVDQLMDVRVEIVDTLVKYFGYTIEATHHEVATAGQGEIDFRFSTLVDTADKVQTLKYVAK
NIAAKHGLVVTFMPKPIYGDNGTGMHTHLSLWTKDGKKNLMYDPNDEYAEISQFGRYVIGGLLTHARALSAIVSPTVNSY
RRLIPGFEAPVYIAWSKGNRSAVIRVPAYYRGMEKAKRIEYRPPDPSTNPYLAFAALLMAALDGVNKKIDPGDPVDENIY
HLTPEKRRQLGIKELPRSLNEALDELESDKEFLKPVFNSSILDTYIDLKRDEARSLQGYPHPMELYYYLDS
>Mature_470_residues
PSTAEDVLKFLKENNIKWVDLQFTDVPGRLHHITIPADEFDLESLKTGFGKLDGSSIRGFTSIYESDMVLLPVPETMTLI
PWSPGVARVLCKVFWGGGKGRFERDPRFIAEEAEKYQTEQGYTSYYGPELEFFMFDKVKLDVSAPQSGTGYKIYAREAPW
SDSGTFVIRFKEGYYPAPPVDQLMDVRVEIVDTLVKYFGYTIEATHHEVATAGQGEIDFRFSTLVDTADKVQTLKYVAKN
IAAKHGLVVTFMPKPIYGDNGTGMHTHLSLWTKDGKKNLMYDPNDEYAEISQFGRYVIGGLLTHARALSAIVSPTVNSYR
RLIPGFEAPVYIAWSKGNRSAVIRVPAYYRGMEKAKRIEYRPPDPSTNPYLAFAALLMAALDGVNKKIDPGDPVDENIYH
LTPEKRRQLGIKELPRSLNEALDELESDKEFLKPVFNSSILDTYIDLKRDEARSLQGYPHPMELYYYLDS

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family

Homologues:

Organism=Homo sapiens, GI221307485, Length=472, Percent_Identity=24.7881355932203, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1790301, Length=478, Percent_Identity=45.1882845188285, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI87081870, Length=396, Percent_Identity=25, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): GLNA_SULSO (P23794)

Other databases:

- EMBL:   X53263
- EMBL:   AE006641
- PIR:   A99180
- PIR:   S11899
- RefSeq:   NP_341906.1
- ProteinModelPortal:   P23794
- SMR:   P23794
- GeneID:   1455506
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0366
- NMPDR:   fig|273057.1.peg.329
- HOGENOM:   HBG553755
- OMA:   YYKGMEK
- PhylomeDB:   P23794
- ProtClustDB:   CLSK883635
- BioCyc:   SSOL273057:SSO0366-MONOMER
- BRENDA:   6.3.1.2
- GO:   GO:0005737
- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- Gene3D:   G3DSA:3.30.590.10
- TIGRFAMs:   TIGR00653

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N; SSF54368 Gln_synt_beta

EC number: =6.3.1.2

Molecular weight: Translated: 53348; Mature: 53217

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00180 GLNA_1; PS00181 GLNA_ATP; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTAEDVLKFLKENNIKWVDLQFTDVPGRLHHITIPADEFDLESLKTGFGKLDGSSIRG
CCCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCH
FTSIYESDMVLLPVPETMTLIPWSPGVARVLCKVFWGGGKGRFERDPRFIAEEAEKYQTE
HHHHHCCCEEEEECCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
QGYTSYYGPELEFFMFDKVKLDVSAPQSGTGYKIYAREAPWSDSGTFVIRFKEGYYPAPP
CCCCCCCCCCEEEEEEEEEEEEECCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCC
VDQLMDVRVEIVDTLVKYFGYTIEATHHEVATAGQGEIDFRFSTLVDTADKVQTLKYVAK
HHHHHHHHHHHHHHHHHHHCCEEEECCHHHHCCCCCCEEEEHHHHHCCHHHHHHHHHHHH
NIAAKHGLVVTFMPKPIYGDNGTGMHTHLSLWTKDGKKNLMYDPNDEYAEISQFGRYVIG
HHHHHCCEEEEEECCCEECCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
GLLTHARALSAIVSPTVNSYRRLIPGFEAPVYIAWSKGNRSAVIRVPAYYRGMEKAKRIE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCHHHHCHHHHHCCC
YRPPDPSTNPYLAFAALLMAALDGVNKKIDPGDPVDENIYHLTPEKRRQLGIKELPRSLN
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHCCCHHHHHHHHH
EALDELESDKEFLKPVFNSSILDTYIDLKRDEARSLQGYPHPMELYYYLDS
HHHHHHHCCHHHHHHHHCCHHHHHHHHCCCHHHHHCCCCCCCEEEEEEECC
>Mature Secondary Structure 
PSTAEDVLKFLKENNIKWVDLQFTDVPGRLHHITIPADEFDLESLKTGFGKLDGSSIRG
CCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCH
FTSIYESDMVLLPVPETMTLIPWSPGVARVLCKVFWGGGKGRFERDPRFIAEEAEKYQTE
HHHHHCCCEEEEECCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
QGYTSYYGPELEFFMFDKVKLDVSAPQSGTGYKIYAREAPWSDSGTFVIRFKEGYYPAPP
CCCCCCCCCCEEEEEEEEEEEEECCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCC
VDQLMDVRVEIVDTLVKYFGYTIEATHHEVATAGQGEIDFRFSTLVDTADKVQTLKYVAK
HHHHHHHHHHHHHHHHHHHCCEEEECCHHHHCCCCCCEEEEHHHHHCCHHHHHHHHHHHH
NIAAKHGLVVTFMPKPIYGDNGTGMHTHLSLWTKDGKKNLMYDPNDEYAEISQFGRYVIG
HHHHHCCEEEEEECCCEECCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
GLLTHARALSAIVSPTVNSYRRLIPGFEAPVYIAWSKGNRSAVIRVPAYYRGMEKAKRIE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCHHHHCHHHHHCCC
YRPPDPSTNPYLAFAALLMAALDGVNKKIDPGDPVDENIYHLTPEKRRQLGIKELPRSLN
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHCCCHHHHHHHHH
EALDELESDKEFLKPVFNSSILDTYIDLKRDEARSLQGYPHPMELYYYLDS
HHHHHHHCCHHHHHHHHCCHHHHHHHHCCCHHHHHCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973523; 11427726