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The map label for this gene is aroE

Identifier: 15897248

GI number: 15897248

Start: 260097

End: 260906

Strand: Direct

Name: aroE

Synonym: SSO0306

Alternate gene names: 15897248

Gene position: 260097-260906 (Clockwise)

Preceding gene: 15897247

Following gene: 15897249

Centisome position: 8.69

GC content: 31.6

Gene sequence:

>810_bases
ATGCTTGAAGAAATAAATTATGATACTAAGTTATTCGGTCTAATAGGTAAAAACATAAAGTACACGCTATCCCCTTATAT
TCATAATTTCTCATTTAGAACACTAGGAATAAATGCAGTTTATCTAGTTTTTGATCTCGACGAAATGAAATTCAAGCGTA
GTATTAGTGGGATATTGGAAATTGCAGAAGGACTTAATGTTACGATACCGTATAAGGATGAAGTAATGAAATATTTGGAT
AATACTGATACGCACTCCACGAGAATTCAAGCTGTAAATACAATATATAAAAAAAGTGGTTATAACACTGATTATTTAGC
AATAAAAAATCTTGTAAGAAAGAAGATTAAGAATGTATCTGGCTACGAATGTTACATATATGGGGCTGGAGGTGCAGCAA
AAGCAGCAGCTTTTGCGTTATCTGAATTAGGATGCTCTAGTATTAGTATTGTGAATAGAACAAAATCAAGGGCTTATGAG
TTAGCTGAATTATTAAATAAGAACGGTTATAACGCGTCAATTAAAGAGAATTGCAACATTACAAATAATATACTTATTGT
CAATAGCACTCCTAATTCTTCTGTAGTCCCAGAGGACTGTGTTAAAAAATCTGATCTTGTTATAGAATTTGTTTATAGAC
CAGTTGAGACTGAGTTAATTAAAAATGCTAAAAAATATGGTATACAATATATAAACGGTCTAGAAATTTTAGTGAATCAA
GCTGTAGAAGCGGAGAAGATATGGTTTAATAAGAGTGTGGCAGATGAAAAGATTATAGAGTATCTTTATGCCAGGGAACT
CGTTTGGTAA

Upstream 100 bases:

>100_bases
AACATAGGAAAGATGTAATTTTGATACCGTTTCCTACTAGAATAGGTAGCTGGAAAAAAGTTGAAGTTCCTCTAGATACC
GTAAAGGGGTTTGCCGAACA

Downstream 100 bases:

>100_bases
ACTATTTAGAATAACCACTTTTGGAGAGAGCCATGGTCCTGCAGTAGGTGTAGTCATAGACGGTGTTCCTGCCGGTTTAC
CATTAACTGTTGAAGATATA

Product: shikimate 5-dehydrogenase (aroE)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MLEEINYDTKLFGLIGKNIKYTLSPYIHNFSFRTLGINAVYLVFDLDEMKFKRSISGILEIAEGLNVTIPYKDEVMKYLD
NTDTHSTRIQAVNTIYKKSGYNTDYLAIKNLVRKKIKNVSGYECYIYGAGGAAKAAAFALSELGCSSISIVNRTKSRAYE
LAELLNKNGYNASIKENCNITNNILIVNSTPNSSVVPEDCVKKSDLVIEFVYRPVETELIKNAKKYGIQYINGLEILVNQ
AVEAEKIWFNKSVADEKIIEYLYARELVW

Sequences:

>Translated_269_residues
MLEEINYDTKLFGLIGKNIKYTLSPYIHNFSFRTLGINAVYLVFDLDEMKFKRSISGILEIAEGLNVTIPYKDEVMKYLD
NTDTHSTRIQAVNTIYKKSGYNTDYLAIKNLVRKKIKNVSGYECYIYGAGGAAKAAAFALSELGCSSISIVNRTKSRAYE
LAELLNKNGYNASIKENCNITNNILIVNSTPNSSVVPEDCVKKSDLVIEFVYRPVETELIKNAKKYGIQYINGLEILVNQ
AVEAEKIWFNKSVADEKIIEYLYARELVW
>Mature_269_residues
MLEEINYDTKLFGLIGKNIKYTLSPYIHNFSFRTLGINAVYLVFDLDEMKFKRSISGILEIAEGLNVTIPYKDEVMKYLD
NTDTHSTRIQAVNTIYKKSGYNTDYLAIKNLVRKKIKNVSGYECYIYGAGGAAKAAAFALSELGCSSISIVNRTKSRAYE
LAELLNKNGYNASIKENCNITNNILIVNSTPNSSVVPEDCVKKSDLVIEFVYRPVETELIKNAKKYGIQYINGLEILVNQ
AVEAEKIWFNKSVADEKIIEYLYARELVW

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1787983, Length=266, Percent_Identity=26.3157894736842, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789675, Length=264, Percent_Identity=25.3787878787879, Blast_Score=74, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320332, Length=267, Percent_Identity=28.0898876404494, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_SULSO (Q980I8)

Other databases:

- EMBL:   AE006641
- PIR:   D90173
- RefSeq:   NP_341853.1
- HSSP:   Q9SQT8
- ProteinModelPortal:   Q980I8
- SMR:   Q980I8
- GeneID:   1455453
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0306
- NMPDR:   fig|273057.1.peg.276
- HOGENOM:   HBG553408
- OMA:   AVAFKIW
- ProtClustDB:   CLSK802661
- BioCyc:   SSOL273057:SSO0306-MONOMER
- BRENDA:   1.1.1.25
- GO:   GO:0005737
- GO:   GO:0005488
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 30472; Mature: 30472

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: ACT_SITE 72-72

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEEINYDTKLFGLIGKNIKYTLSPYIHNFSFRTLGINAVYLVFDLDEMKFKRSISGILE
CCCCCCCCHHHHHHHCCCCEEEECHHHHCCEEEEECCEEEEEEEECHHHHHHHHHHHHHH
IAEGLNVTIPYKDEVMKYLDNTDTHSTRIQAVNTIYKKSGYNTDYLAIKNLVRKKIKNVS
HHCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
GYECYIYGAGGAAKAAAFALSELGCSSISIVNRTKSRAYELAELLNKNGYNASIKENCNI
CCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
TNNILIVNSTPNSSVVPEDCVKKSDLVIEFVYRPVETELIKNAKKYGIQYINGLEILVNQ
CCCEEEEECCCCCCCCCHHHHCCCCEEEEEHHCCHHHHHHHHHHHHCHHHHHHHHHHHHH
AVEAEKIWFNKSVADEKIIEYLYARELVW
HHHHHHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLEEINYDTKLFGLIGKNIKYTLSPYIHNFSFRTLGINAVYLVFDLDEMKFKRSISGILE
CCCCCCCCHHHHHHHCCCCEEEECHHHHCCEEEEECCEEEEEEEECHHHHHHHHHHHHHH
IAEGLNVTIPYKDEVMKYLDNTDTHSTRIQAVNTIYKKSGYNTDYLAIKNLVRKKIKNVS
HHCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
GYECYIYGAGGAAKAAAFALSELGCSSISIVNRTKSRAYELAELLNKNGYNASIKENCNI
CCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
TNNILIVNSTPNSSVVPEDCVKKSDLVIEFVYRPVETELIKNAKKYGIQYINGLEILVNQ
CCCEEEEECCCCCCCCCHHHHCCCCEEEEEHHCCHHHHHHHHHHHHCHHHHHHHHHHHHH
AVEAEKIWFNKSVADEKIIEYLYARELVW
HHHHHHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726