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The map label for this gene is thiF [H]

Identifier: 15897242

GI number: 15897242

Start: 254333

End: 255334

Strand: Reverse

Name: thiF [H]

Synonym: SSO0298

Alternate gene names: 15897242

Gene position: 255334-254333 (Counterclockwise)

Preceding gene: 15897253

Following gene: 15897241

Centisome position: 8.53

GC content: 32.93

Gene sequence:

>1002_bases
TTGTCCAATTCCTCTATTGATATTGGAGTTCCATCACTCCCTCCACCTTTTTCCATTGGAACAATATCAATCCCCACTTT
TAGTAGGCAACACTTATTTTTAAAGATTATTCTACATTATAATCTTGTGGAAAGATATTCTAGGCAACTAATAGTTTTAG
GATTAGGAATTCAACAGAGGTTAAATGAGTTAAAGATATTAATTGCAGGCTGTGGAGCTTTAGGTACTGCCGTTGCAGAG
TTGCTAGCTAGGCTAGGAGTTAAAGAGTTAACTATAGTAGATGCAGATGTGGTAGATATAACTAATCTACATAGGGTCCA
TTTATTTGATGAAAATGATGTAGGCAAACCTAAAGCTGAAGTTTGCGCTAAAAAAATATCCCTTATAAATTCTTCAATCA
AAATTAATTATATTATAGATATACTCGACGAGGAGAACGTCGAGAGGCTAATTTCTGATAAGGATTACGTGTTCGATGCA
TTAGACAGCTTGTACTACAAGCTATTGTTAAATGATGCTATAGTAAAATTAGGAAAAATCCTAATATATGGAGGAATTAA
TGGTGAATACGGTTCTGCTAAACTGATAGACCCTAGTCAAACCTCCTGTTTATCTTGTTTTATTGACTATTCAGATCAAG
ACGAAATTGGGAATTCATGTGATGTTATTGGAACTACTCCACTAATAGTAGAATTAACAGCTACACTTCAAGTTAACTTA
ATGCTTAATCACTTGAGAGGCAATCCGGATTATTCATTATTCTATATAGATTCTAGAGAATTAAAAATTGAGAGAATTAA
CATCGAGAAGAACTCACAATGTAAGACATGCGTTCTAAATGAGTATCCTTTTCTATATCAAAAGATAAGTAAACCTAAAT
GTGGAATATTTAGAACTAATATGAAAATTCCTGAGTTAGATGAGCCCAAAGTGTTTAAAAATCTTGGGAATGTAACTTTA
TGCTATCCTAGTATTGGATGTTTTGAGAAAAGGGGAAGATAA

Upstream 100 bases:

>100_bases
TATCTCTATGCTACTTAGGGACGATCCCACATGTATTGTTTGATCATAAAATAGCATTTTTATTACATTTCTTCTTGCCT
TTTCAGCTACTTGTCTTAAC

Downstream 100 bases:

>100_bases
TGGATAAGAAATACATTGCAGCTATTTTTCTTCCGTTTATAGTGATCATAGTCTATGCATACATATTTAAGATTAATATA
ATATATGTTTTACAAGAAGA

Product: thiamine biosynthesis protein related protein (thiF)

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSNSSIDIGVPSLPPPFSIGTISIPTFSRQHLFLKIILHYNLVERYSRQLIVLGLGIQQRLNELKILIAGCGALGTAVAE
LLARLGVKELTIVDADVVDITNLHRVHLFDENDVGKPKAEVCAKKISLINSSIKINYIIDILDEENVERLISDKDYVFDA
LDSLYYKLLLNDAIVKLGKILIYGGINGEYGSAKLIDPSQTSCLSCFIDYSDQDEIGNSCDVIGTTPLIVELTATLQVNL
MLNHLRGNPDYSLFYIDSRELKIERINIEKNSQCKTCVLNEYPFLYQKISKPKCGIFRTNMKIPELDEPKVFKNLGNVTL
CYPSIGCFEKRGR

Sequences:

>Translated_333_residues
MSNSSIDIGVPSLPPPFSIGTISIPTFSRQHLFLKIILHYNLVERYSRQLIVLGLGIQQRLNELKILIAGCGALGTAVAE
LLARLGVKELTIVDADVVDITNLHRVHLFDENDVGKPKAEVCAKKISLINSSIKINYIIDILDEENVERLISDKDYVFDA
LDSLYYKLLLNDAIVKLGKILIYGGINGEYGSAKLIDPSQTSCLSCFIDYSDQDEIGNSCDVIGTTPLIVELTATLQVNL
MLNHLRGNPDYSLFYIDSRELKIERINIEKNSQCKTCVLNEYPFLYQKISKPKCGIFRTNMKIPELDEPKVFKNLGNVTL
CYPSIGCFEKRGR
>Mature_332_residues
SNSSIDIGVPSLPPPFSIGTISIPTFSRQHLFLKIILHYNLVERYSRQLIVLGLGIQQRLNELKILIAGCGALGTAVAEL
LARLGVKELTIVDADVVDITNLHRVHLFDENDVGKPKAEVCAKKISLINSSIKINYIIDILDEENVERLISDKDYVFDAL
DSLYYKLLLNDAIVKLGKILIYGGINGEYGSAKLIDPSQTSCLSCFIDYSDQDEIGNSCDVIGTTPLIVELTATLQVNLM
LNHLRGNPDYSLFYIDSRELKIERINIEKNSQCKTCVLNEYPFLYQKISKPKCGIFRTNMKIPELDEPKVFKNLGNVTLC
YPSIGCFEKRGR

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=150, Percent_Identity=36.6666666666667, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI38045948, Length=166, Percent_Identity=32.5301204819277, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI38045944, Length=169, Percent_Identity=28.9940828402367, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI38045942, Length=169, Percent_Identity=28.9940828402367, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI150417996, Length=181, Percent_Identity=27.0718232044199, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI87082356, Length=238, Percent_Identity=29.4117647058824, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787048, Length=239, Percent_Identity=25.9414225941423, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1789177, Length=171, Percent_Identity=31.5789473684211, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17540406, Length=241, Percent_Identity=31.9502074688797, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71984476, Length=166, Percent_Identity=29.5180722891566, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI86565431, Length=175, Percent_Identity=27.4285714285714, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI86565433, Length=175, Percent_Identity=27.4285714285714, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=218, Percent_Identity=29.3577981651376, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6322639, Length=177, Percent_Identity=31.0734463276836, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6325323, Length=166, Percent_Identity=31.9277108433735, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6320598, Length=163, Percent_Identity=26.9938650306748, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24582879, Length=271, Percent_Identity=27.6752767527675, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20129973, Length=159, Percent_Identity=28.9308176100629, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 37280; Mature: 37149

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSSIDIGVPSLPPPFSIGTISIPTFSRQHLFLKIILHYNLVERYSRQLIVLGLGIQQR
CCCCCEEECCCCCCCCCCCCEEECCCCCCCCEEEHHHHHHHHHHHHCCEEEEEECCHHHH
LNELKILIAGCGALGTAVAELLARLGVKELTIVDADVVDITNLHRVHLFDENDVGKPKAE
HHHHEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCEEECCCCEEEEEECCCCCCCCHHH
VCAKKISLINSSIKINYIIDILDEENVERLISDKDYVFDALDSLYYKLLLNDAIVKLGKI
HHHHHHHHHCCCEEEEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCEE
LIYGGINGEYGSAKLIDPSQTSCLSCFIDYSDQDEIGNSCDVIGTTPLIVELTATLQVNL
EEEECCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCEEECCCEEEEEEEEEEEEE
MLNHLRGNPDYSLFYIDSRELKIERINIEKNSQCKTCVLNEYPFLYQKISKPKCGIFRTN
EEEECCCCCCEEEEEEECCEEEEEEEECCCCCCCCEEEECCCCHHHHHHCCCCCCEEECC
MKIPELDEPKVFKNLGNVTLCYPSIGCFEKRGR
CCCCCCCCCHHHHHCCCEEEEECCCCCHHCCCC
>Mature Secondary Structure 
SNSSIDIGVPSLPPPFSIGTISIPTFSRQHLFLKIILHYNLVERYSRQLIVLGLGIQQR
CCCCEEECCCCCCCCCCCCEEECCCCCCCCEEEHHHHHHHHHHHHCCEEEEEECCHHHH
LNELKILIAGCGALGTAVAELLARLGVKELTIVDADVVDITNLHRVHLFDENDVGKPKAE
HHHHEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCEEECCCCEEEEEECCCCCCCCHHH
VCAKKISLINSSIKINYIIDILDEENVERLISDKDYVFDALDSLYYKLLLNDAIVKLGKI
HHHHHHHHHCCCEEEEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCEE
LIYGGINGEYGSAKLIDPSQTSCLSCFIDYSDQDEIGNSCDVIGTTPLIVELTATLQVNL
EEEECCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCEEECCCEEEEEEEEEEEEE
MLNHLRGNPDYSLFYIDSRELKIERINIEKNSQCKTCVLNEYPFLYQKISKPKCGIFRTN
EEEECCCCCCEEEEEEECCEEEEEEEECCCCCCCCEEEECCCCHHHHHHCCCCCCEEECC
MKIPELDEPKVFKNLGNVTLCYPSIGCFEKRGR
CCCCCCCCCHHHHHCCCEEEEECCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]