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The map label for this gene is gatB [H]

Identifier: 15897180

GI number: 15897180

Start: 200640

End: 202016

Strand: Direct

Name: gatB [H]

Synonym: SSO0232

Alternate gene names: 15897180

Gene position: 200640-202016 (Clockwise)

Preceding gene: 15897179

Following gene: 15897181

Centisome position: 6.71

GC content: 33.99

Gene sequence:

>1377_bases
TTGAAAACTAAACTATTCTGTTCATGTCCATCGGATTATACTGGAAAAGACCCGAATACAGTAACTTGTCCCGTATGTTT
AGGATTACCTGGGGCTATTCCCGTATTAAATGAAAATGCAGTAAAATACGGTATCTTAACTGCATTAGCCCTTAACTGTC
AAATTGCTGAAAAATTGATTTTCGATAGAAAACACTATTTTTATCCAGATATGTCTAAAAATTATCAGATATCTCAGTAT
GATGGACCAGGTAGTATGGCCATAGCCAAAGACGGTTATATCAAATTAAATGATAAAATCATAAGAATTAGAAGAATAAA
TATAGAAGAAGATCCGGCAAAGATAGTCTACCCTACTGGTTCTATACTAACTAGCAAATATACGTTACTAGACTATAATA
GATCTGGTACACCATTGTTAGAAATTGTAACAGAACCAGATCTAAGGTCTGCTAAAGAAGCCAGATTCTTCCTAGAAAAA
TTAAGGTCAATCCTAGAACATTTAGGTGTATGTGATTGTGGTATTGAAGGTGCAATGAAAGCTGATGTAAATATATCAGT
AAAAGGAGGAGAGAGAGTGGAAGTTAAGAATGTGGGTTCTCCTAAAGACGTTGAGGATGCTATAAATTATGAAATTGCAA
GACAGAGAGCTAGTATACTACAAGGTATCAAGATAGAAAGAGAGACTAGACATTGGGATAACGAAAGGAGAGTTACAGTA
CCTTTACGAACTAAAGAGACTGAGGAGGATTATAGATATTTTCCAGATCCAGATTTACCGCCATACTCAATAACACCAGA
GCTTATCGAGAAGTTTAAACGTGAAATGCCAGAGTTACCAGACCAGAGAGCGGAGAGATTTGTAAAACAATACAACGTTA
CTCCATATCAAGCTCAAGTTTTAGTTAATGAAAAAGCGTTAGCAGATTTATTTGAAGAAATCACAAACAACTATAAAAAC
TATGCCAAAGTCGCAAATCTGCTAATTAACGATTACATGAGATGGCTTAACGAAAATAATATCACAGTAACACAAAGTAA
AGCCAAAGCTAATCATATCATTGAACTATTTGAATACTTAGATTCTGGCTTAATATCAATAAAAATAGTAAAAGAACTAC
TACCTGAAATGATCTTAACTGGAAAAACTCCGGGACAACTAATTAAGGAAAAGGGTATGACAAACATCAAAGATGAGAAC
TACTTAGAAAGGATTATAGATGAAGTATTAAATGAAGAGAAAGAGGCTGTAGAGAAAGCTAAGAGAGATCCAAAAGTTGT
AAATTATTTAGTAGGTATGGTAATGAAGAAGACGGGGAAAAGAGCTGACCCTCAAATGACAGTTGAAATAATAAAAAAGA
AGCTAGGTTTAACTTAA

Upstream 100 bases:

>100_bases
TTTGCCTGGTCTCGGAGACGCTGGAGATAGAGCATTTGGGTGATGAGATACGGATGATGATGTAAAAATAGGATTAGAAG
TTCATGTTCATATAACTGCA

Downstream 100 bases:

>100_bases
CATGGAGATTTTTATGTTACAGAGTAATCAAATAAAGGAAAAAGAATTACGGCTTAGGCTATTTAGTGTAGATATCTTAA
AAGAATTGAAAAACATTTAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKTKLFCSCPSDYTGKDPNTVTCPVCLGLPGAIPVLNENAVKYGILTALALNCQIAEKLIFDRKHYFYPDMSKNYQISQY
DGPGSMAIAKDGYIKLNDKIIRIRRINIEEDPAKIVYPTGSILTSKYTLLDYNRSGTPLLEIVTEPDLRSAKEARFFLEK
LRSILEHLGVCDCGIEGAMKADVNISVKGGERVEVKNVGSPKDVEDAINYEIARQRASILQGIKIERETRHWDNERRVTV
PLRTKETEEDYRYFPDPDLPPYSITPELIEKFKREMPELPDQRAERFVKQYNVTPYQAQVLVNEKALADLFEEITNNYKN
YAKVANLLINDYMRWLNENNITVTQSKAKANHIIELFEYLDSGLISIKIVKELLPEMILTGKTPGQLIKEKGMTNIKDEN
YLERIIDEVLNEEKEAVEKAKRDPKVVNYLVGMVMKKTGKRADPQMTVEIIKKKLGLT

Sequences:

>Translated_458_residues
MKTKLFCSCPSDYTGKDPNTVTCPVCLGLPGAIPVLNENAVKYGILTALALNCQIAEKLIFDRKHYFYPDMSKNYQISQY
DGPGSMAIAKDGYIKLNDKIIRIRRINIEEDPAKIVYPTGSILTSKYTLLDYNRSGTPLLEIVTEPDLRSAKEARFFLEK
LRSILEHLGVCDCGIEGAMKADVNISVKGGERVEVKNVGSPKDVEDAINYEIARQRASILQGIKIERETRHWDNERRVTV
PLRTKETEEDYRYFPDPDLPPYSITPELIEKFKREMPELPDQRAERFVKQYNVTPYQAQVLVNEKALADLFEEITNNYKN
YAKVANLLINDYMRWLNENNITVTQSKAKANHIIELFEYLDSGLISIKIVKELLPEMILTGKTPGQLIKEKGMTNIKDEN
YLERIIDEVLNEEKEAVEKAKRDPKVVNYLVGMVMKKTGKRADPQMTVEIIKKKLGLT
>Mature_458_residues
MKTKLFCSCPSDYTGKDPNTVTCPVCLGLPGAIPVLNENAVKYGILTALALNCQIAEKLIFDRKHYFYPDMSKNYQISQY
DGPGSMAIAKDGYIKLNDKIIRIRRINIEEDPAKIVYPTGSILTSKYTLLDYNRSGTPLLEIVTEPDLRSAKEARFFLEK
LRSILEHLGVCDCGIEGAMKADVNISVKGGERVEVKNVGSPKDVEDAINYEIARQRASILQGIKIERETRHWDNERRVTV
PLRTKETEEDYRYFPDPDLPPYSITPELIEKFKREMPELPDQRAERFVKQYNVTPYQAQVLVNEKALADLFEEITNNYKN
YAKVANLLINDYMRWLNENNITVTQSKAKANHIIELFEYLDSGLISIKIVKELLPEMILTGKTPGQLIKEKGMTNIKDEN
YLERIIDEVLNEEKEAVEKAKRDPKVVNYLVGMVMKKTGKRADPQMTVEIIKKKLGLT

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=484, Percent_Identity=35.3305785123967, Blast_Score=260, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI115534094, Length=290, Percent_Identity=37.2413793103448, Blast_Score=174, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6319391, Length=378, Percent_Identity=33.0687830687831, Blast_Score=184, Evalue=2e-47,
Organism=Drosophila melanogaster, GI45550808, Length=482, Percent_Identity=32.9875518672199, Blast_Score=248, Evalue=5e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52338; Mature: 52338

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKLFCSCPSDYTGKDPNTVTCPVCLGLPGAIPVLNENAVKYGILTALALNCQIAEKLI
CCCCEEEECCCCCCCCCCCEEEEHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FDRKHYFYPDMSKNYQISQYDGPGSMAIAKDGYIKLNDKIIRIRRINIEEDPAKIVYPTG
HCCCCCCCCCCCCCEEEEEECCCCCEEEECCCEEEECCEEEEEEEECCCCCCCEEEECCC
SILTSKYTLLDYNRSGTPLLEIVTEPDLRSAKEARFFLEKLRSILEHLGVCDCGIEGAMK
CHHCCEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
ADVNISVKGGERVEVKNVGSPKDVEDAINYEIARQRASILQGIKIERETRHWDNERRVTV
ECEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEE
PLRTKETEEDYRYFPDPDLPPYSITPELIEKFKREMPELPDQRAERFVKQYNVTPYQAQV
EECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEE
LVNEKALADLFEEITNNYKNYAKVANLLINDYMRWLNENNITVTQSKAKANHIIELFEYL
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHH
DSGLISIKIVKELLPEMILTGKTPGQLIKEKGMTNIKDENYLERIIDEVLNEEKEAVEKA
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHHHHH
KRDPKVVNYLVGMVMKKTGKRADPQMTVEIIKKKLGLT
HCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTKLFCSCPSDYTGKDPNTVTCPVCLGLPGAIPVLNENAVKYGILTALALNCQIAEKLI
CCCCEEEECCCCCCCCCCCEEEEHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FDRKHYFYPDMSKNYQISQYDGPGSMAIAKDGYIKLNDKIIRIRRINIEEDPAKIVYPTG
HCCCCCCCCCCCCCEEEEEECCCCCEEEECCCEEEECCEEEEEEEECCCCCCCEEEECCC
SILTSKYTLLDYNRSGTPLLEIVTEPDLRSAKEARFFLEKLRSILEHLGVCDCGIEGAMK
CHHCCEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
ADVNISVKGGERVEVKNVGSPKDVEDAINYEIARQRASILQGIKIERETRHWDNERRVTV
ECEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEE
PLRTKETEEDYRYFPDPDLPPYSITPELIEKFKREMPELPDQRAERFVKQYNVTPYQAQV
EECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEE
LVNEKALADLFEEITNNYKNYAKVANLLINDYMRWLNENNITVTQSKAKANHIIELFEYL
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHH
DSGLISIKIVKELLPEMILTGKTPGQLIKEKGMTNIKDENYLERIIDEVLNEEKEAVEKA
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHHHHH
KRDPKVVNYLVGMVMKKTGKRADPQMTVEIIKKKLGLT
HCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]