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The map label for this gene is hemB [H]

Identifier: 15897132

GI number: 15897132

Start: 152828

End: 153853

Strand: Direct

Name: hemB [H]

Synonym: SSO0181

Alternate gene names: 15897132

Gene position: 152828-153853 (Clockwise)

Preceding gene: 15897131

Following gene: 15897133

Centisome position: 5.11

GC content: 35.96

Gene sequence:

>1026_bases
TTGATATTTTTAAAGATGGTAACATTTCCGGTTTTGAGACCGAGAAGATTAAGGAAAAGCAAGTTAGTGAGAGATCTAGT
ATCTGAAACATCTATTTCACAAAACGATTTAGTACTTCCAGTATTTATAAAGGAAAACATAAATGAGCCAGAGCAAATTG
CTTCGATGCCAGGTATATATAGATATCCGCCAAATGATAAGTTAATCAACTATCTACAAGATAGTTACGAAAACGGAATT
CGAAGTGTAATATTGTTTGGAATTCCTTCTTATAAAGACGATATAGCATCTTCAGCCTATGATAAAAATGGTATAATACA
GAGAGCGGTTAGAATAATAAAGGATAGTTTTAAGGATAAGATTATAGTAATCACAGATGAGTGTACTGATGAGTATATGG
CTAATGGTCATTGTGGTCTTGTTAAATATACTAACGGGTGTTATGTGGTAGATAATGATGAAAGTCTAAAGATTCATGCT
AAGATTGCATTAAGTCAAGCTGAAGCTGGTGCAGACGTTATAGCTCCATCCAGCATGATGGATGGAGTAGTTAGGGCTAT
AAGAGATGCGCTAGATAGTGCCGGCTACATTGATACATTAATATTGTCATATAGTGTAAAGTACGCTTCAGTAATGTATG
GTCCCTTTAGGGATGCAGCGTACTCTAAACCCGCATTTGGAGATAGAAGGGGATATCAGATGGACCCTAGAAATGCATTT
GAGGCAATAAAAGAAGCGAGACTAGATATAGAGGAAGGTGCTGATATTTTGATGGTTAAACCTGCCCATACATATTTAGA
TGTAATAAGATTAGTGAAAGACCACTTTCCAGAATATCCGCTAGCTGCATATCATGTTAGTGGTGAATATAGTATGATAA
AGGCAGCTGCAATTAATGGATGGATAGACGAAAAGACTGCAGTCTTAGAAATTACTACTGCAATAAAAAGGGCAGGGGCT
GATTTAATCATTACTTATTATGCTAACGATATTGCTAGATGGATAAAGGACGGTGTACCATTTTGA

Upstream 100 bases:

>100_bases
ACTAGATCAATCATTAAAAAGTCATTCCAACCCCTTTTCGATAATATAAGATCACTAATATTTAACGGAGAAAATTCGAT
AAACTACATTAACTTTCTAA

Downstream 100 bases:

>100_bases
ATAGCGAGGAACTATGGGCTCAAGCTAAGCAATTATTTGCTGGAGGAGTAAATAGTCCAGTTAGAGCAGCAGTAAAGCCA
TTCCCCTTTTATGTAGAAAG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MIFLKMVTFPVLRPRRLRKSKLVRDLVSETSISQNDLVLPVFIKENINEPEQIASMPGIYRYPPNDKLINYLQDSYENGI
RSVILFGIPSYKDDIASSAYDKNGIIQRAVRIIKDSFKDKIIVITDECTDEYMANGHCGLVKYTNGCYVVDNDESLKIHA
KIALSQAEAGADVIAPSSMMDGVVRAIRDALDSAGYIDTLILSYSVKYASVMYGPFRDAAYSKPAFGDRRGYQMDPRNAF
EAIKEARLDIEEGADILMVKPAHTYLDVIRLVKDHFPEYPLAAYHVSGEYSMIKAAAINGWIDEKTAVLEITTAIKRAGA
DLIITYYANDIARWIKDGVPF

Sequences:

>Translated_341_residues
MIFLKMVTFPVLRPRRLRKSKLVRDLVSETSISQNDLVLPVFIKENINEPEQIASMPGIYRYPPNDKLINYLQDSYENGI
RSVILFGIPSYKDDIASSAYDKNGIIQRAVRIIKDSFKDKIIVITDECTDEYMANGHCGLVKYTNGCYVVDNDESLKIHA
KIALSQAEAGADVIAPSSMMDGVVRAIRDALDSAGYIDTLILSYSVKYASVMYGPFRDAAYSKPAFGDRRGYQMDPRNAF
EAIKEARLDIEEGADILMVKPAHTYLDVIRLVKDHFPEYPLAAYHVSGEYSMIKAAAINGWIDEKTAVLEITTAIKRAGA
DLIITYYANDIARWIKDGVPF
>Mature_341_residues
MIFLKMVTFPVLRPRRLRKSKLVRDLVSETSISQNDLVLPVFIKENINEPEQIASMPGIYRYPPNDKLINYLQDSYENGI
RSVILFGIPSYKDDIASSAYDKNGIIQRAVRIIKDSFKDKIIVITDECTDEYMANGHCGLVKYTNGCYVVDNDESLKIHA
KIALSQAEAGADVIAPSSMMDGVVRAIRDALDSAGYIDTLILSYSVKYASVMYGPFRDAAYSKPAFGDRRGYQMDPRNAF
EAIKEARLDIEEGADILMVKPAHTYLDVIRLVKDHFPEYPLAAYHVSGEYSMIKAAAINGWIDEKTAVLEITTAIKRAGA
DLIITYYANDIARWIKDGVPF

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=314, Percent_Identity=42.9936305732484, Blast_Score=256, Evalue=3e-68,
Organism=Escherichia coli, GI87081728, Length=323, Percent_Identity=49.5356037151703, Blast_Score=291, Evalue=4e-80,
Organism=Saccharomyces cerevisiae, GI6321398, Length=320, Percent_Identity=39.6875, Blast_Score=226, Evalue=6e-60,
Organism=Drosophila melanogaster, GI21358291, Length=302, Percent_Identity=43.046357615894, Blast_Score=228, Evalue=4e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 38263; Mature: 38263

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFLKMVTFPVLRPRRLRKSKLVRDLVSETSISQNDLVLPVFIKENINEPEQIASMPGIY
CEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHCCCCCE
RYPPNDKLINYLQDSYENGIRSVILFGIPSYKDDIASSAYDKNGIIQRAVRIIKDSFKDK
ECCCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCE
IIVITDECTDEYMANGHCGLVKYTNGCYVVDNDESLKIHAKIALSQAEAGADVIAPSSMM
EEEEECCCCHHHHCCCCEEEEEEECCEEEEECCCCEEEEEEEEEEHHHCCCCCCCCHHHH
DGVVRAIRDALDSAGYIDTLILSYSVKYASVMYGPFRDAAYSKPAFGDRRGYQMDPRNAF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCHHCCCCCCCCCCCCCCCCCHHHHH
EAIKEARLDIEEGADILMVKPAHTYLDVIRLVKDHFPEYPLAAYHVSGEYSMIKAAAING
HHHHHHHCCHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCC
WIDEKTAVLEITTAIKRAGADLIITYYANDIARWIKDGVPF
CCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIFLKMVTFPVLRPRRLRKSKLVRDLVSETSISQNDLVLPVFIKENINEPEQIASMPGIY
CEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHCCCCCE
RYPPNDKLINYLQDSYENGIRSVILFGIPSYKDDIASSAYDKNGIIQRAVRIIKDSFKDK
ECCCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCE
IIVITDECTDEYMANGHCGLVKYTNGCYVVDNDESLKIHAKIALSQAEAGADVIAPSSMM
EEEEECCCCHHHHCCCCEEEEEEECCEEEEECCCCEEEEEEEEEEHHHCCCCCCCCHHHH
DGVVRAIRDALDSAGYIDTLILSYSVKYASVMYGPFRDAAYSKPAFGDRRGYQMDPRNAF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCHHCCCCCCCCCCCCCCCCCHHHHH
EAIKEARLDIEEGADILMVKPAHTYLDVIRLVKDHFPEYPLAAYHVSGEYSMIKAAAING
HHHHHHHCCHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCC
WIDEKTAVLEITTAIKRAGADLIITYYANDIARWIKDGVPF
CCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]