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The map label for this gene is rad2

Identifier: 15897130

GI number: 15897130

Start: 150044

End: 150952

Strand: Direct

Name: rad2

Synonym: SSO0179

Alternate gene names: 15897130

Gene position: 150044-150952 (Clockwise)

Preceding gene: 15897129

Following gene: 15897131

Centisome position: 5.01

GC content: 34.21

Gene sequence:

>909_bases
ATGGATTCACAGGGGAGAGTAACTAGTCATCTAAGTGGATTATTTTATAGAACGATAAATATCCTAGAAGAAGGTGTCAT
ACCAATCTACGTCTTTGATGGTAAACCGCCAGAGCAAAAAAGTGAAGAGTTAGAAAGAAGGAGAAAGGCAAAAGAAGAAG
CTGAGAGAAAGTTAGAAAGAGCCAAAAGCGAAGGGAAGATAGAGGAGCTAAGGAAATATTCACAAGCTATACTTAGATTA
TCAAATATCATGGTAGAAGAAAGTAAAAAATTGTTGAGAGCTATGGGAATACCTATAGTTCAAGCACCTTCTGAAGGAGA
GGCTGAGGCTGCATATCTGAATAAATTAGGATTAAGCTGGGCAGCAGCAAGTCAAGATTATGATGCGATACTTTTCGGAG
CTAAAAGGCTAGTAAGAAATTTGACAATTACAGGGAAAAGAAAGCTTCCTAACAAGGACGTATATGTGGAGATAAAACCA
GAATTAATAGAAACGGAAATCTTACTTAAAAAATTAGGAATAACAAGGGAACAACTAATTGATATAGGCATATTAATTGG
AACAGACTATAATCCTGATGGAATAAGGGGCATAGGACCAGAAAGAGCACTAAAGATAATAAAAAAATACGGCAAGATAG
AAAAAGCAATGGAATATGGTGAAATTTCAAAAAAGGATATTAATTTCAATATCGATGAAATTAGAGGTTTATTTTTGAAT
CCACAAGTAGTTAAACCAGAGGAAGCTTTAGATTTGAATGAACCTAACGGAGAAGATATAATTAATATCTTAGTATATGA
ACATAACTTCAGTGAAGAAAGAGTGAAGAATGGAATTGAAAGATTAACTAAAGCAATAAAAGAAGCTAAAGGAGCGTCTA
GACAAACAGGATTGGACAGATGGTTTTAA

Upstream 100 bases:

>100_bases
TTCGTTTTCTGAACTTAAAGGAAAGAGGGTAAGTATCGATGGATATAATGCGTTATATCAGTTTTTAGCTGCAATAAGAC
AACCGGATGGAACTCCCTTA

Downstream 100 bases:

>100_bases
TACTTTAAAGTTAAACTTAAAGTTATCCATTAATATCTACTACCTTGTGATTACAAAGGATTATTATCCAATTTTTATAG
ACCTTTCTAATTTTCGTATA

Product: flap endonuclease-1

Products: NA

Alternate protein names: FEN-1; Flap structure-specific endonuclease 1

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MDSQGRVTSHLSGLFYRTINILEEGVIPIYVFDGKPPEQKSEELERRRKAKEEAERKLERAKSEGKIEELRKYSQAILRL
SNIMVEESKKLLRAMGIPIVQAPSEGEAEAAYLNKLGLSWAAASQDYDAILFGAKRLVRNLTITGKRKLPNKDVYVEIKP
ELIETEILLKKLGITREQLIDIGILIGTDYNPDGIRGIGPERALKIIKKYGKIEKAMEYGEISKKDINFNIDEIRGLFLN
PQVVKPEEALDLNEPNGEDIINILVYEHNFSEERVKNGIERLTKAIKEAKGASRQTGLDRWF

Sequences:

>Translated_302_residues
MDSQGRVTSHLSGLFYRTINILEEGVIPIYVFDGKPPEQKSEELERRRKAKEEAERKLERAKSEGKIEELRKYSQAILRL
SNIMVEESKKLLRAMGIPIVQAPSEGEAEAAYLNKLGLSWAAASQDYDAILFGAKRLVRNLTITGKRKLPNKDVYVEIKP
ELIETEILLKKLGITREQLIDIGILIGTDYNPDGIRGIGPERALKIIKKYGKIEKAMEYGEISKKDINFNIDEIRGLFLN
PQVVKPEEALDLNEPNGEDIINILVYEHNFSEERVKNGIERLTKAIKEAKGASRQTGLDRWF
>Mature_302_residues
MDSQGRVTSHLSGLFYRTINILEEGVIPIYVFDGKPPEQKSEELERRRKAKEEAERKLERAKSEGKIEELRKYSQAILRL
SNIMVEESKKLLRAMGIPIVQAPSEGEAEAAYLNKLGLSWAAASQDYDAILFGAKRLVRNLTITGKRKLPNKDVYVEIKP
ELIETEILLKKLGITREQLIDIGILIGTDYNPDGIRGIGPERALKIIKKYGKIEKAMEYGEISKKDINFNIDEIRGLFLN
PQVVKPEEALDLNEPNGEDIINILVYEHNFSEERVKNGIERLTKAIKEAKGASRQTGLDRWF

Specific function: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymeras

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily

Homologues:

Organism=Homo sapiens, GI4758356, Length=304, Percent_Identity=39.8026315789474, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI194018535, Length=199, Percent_Identity=32.1608040201005, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI194018531, Length=199, Percent_Identity=32.1608040201005, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI284055276, Length=209, Percent_Identity=28.7081339712919, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI284172361, Length=209, Percent_Identity=28.7081339712919, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI284055278, Length=209, Percent_Identity=28.7081339712919, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI51988900, Length=127, Percent_Identity=33.8582677165354, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17510005, Length=301, Percent_Identity=36.5448504983389, Blast_Score=200, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI17555016, Length=209, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322736, Length=306, Percent_Identity=39.5424836601307, Blast_Score=200, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6321697, Length=136, Percent_Identity=30.8823529411765, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320469, Length=188, Percent_Identity=29.7872340425532, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324607, Length=216, Percent_Identity=26.8518518518519, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17647423, Length=303, Percent_Identity=39.6039603960396, Blast_Score=211, Evalue=7e-55,
Organism=Drosophila melanogaster, GI24658219, Length=205, Percent_Identity=30.7317073170732, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI78706854, Length=134, Percent_Identity=32.089552238806, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI78706850, Length=134, Percent_Identity=32.089552238806, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI78706852, Length=134, Percent_Identity=32.089552238806, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17137168, Length=194, Percent_Identity=27.8350515463918, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FEN_SULSO (Q980U8)

Other databases:

- EMBL:   AE006641
- PDB:   2IZO
- PDBsum:   2IZO
- ProteinModelPortal:   Q980U8
- SMR:   Q980U8
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0179
- NMPDR:   fig|273057.1.peg.158
- HOGENOM:   HBG316651
- OMA:   DYDSLLF
- PhylomeDB:   Q980U8
- ProtClustDB:   PRK03980
- BioCyc:   SSOL273057:SSO0179-MONOMER
- HAMAP:   MF_00614
- InterPro:   IPR020045
- InterPro:   IPR019973
- InterPro:   IPR008918
- InterPro:   IPR006086
- InterPro:   IPR019974
- InterPro:   IPR006085
- InterPro:   IPR006084
- PANTHER:   PTHR11081
- PRINTS:   PR00853
- SMART:   SM00279
- SMART:   SM00484
- SMART:   SM00485
- TIGRFAMs:   TIGR03674

Pfam domain/function: PF00867 XPG_I; PF00752 XPG_N; SSF47807 5_3_exo_C

EC number: 3.-.-.-

Molecular weight: Translated: 34333; Mature: 34333

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS00841 XPG_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSQGRVTSHLSGLFYRTINILEEGVIPIYVFDGKPPEQKSEELERRRKAKEEAERKLER
CCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
AKSEGKIEELRKYSQAILRLSNIMVEESKKLLRAMGIPIVQAPSEGEAEAAYLNKLGLSW
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCHH
AAASQDYDAILFGAKRLVRNLTITGKRKLPNKDVYVEIKPELIETEILLKKLGITREQLI
HHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHH
DIGILIGTDYNPDGIRGIGPERALKIIKKYGKIEKAMEYGEISKKDINFNIDEIRGLFLN
HEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEECC
PQVVKPEEALDLNEPNGEDIINILVYEHNFSEERVKNGIERLTKAIKEAKGASRQTGLDR
CCEECCHHHCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCC
WF
CC
>Mature Secondary Structure
MDSQGRVTSHLSGLFYRTINILEEGVIPIYVFDGKPPEQKSEELERRRKAKEEAERKLER
CCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
AKSEGKIEELRKYSQAILRLSNIMVEESKKLLRAMGIPIVQAPSEGEAEAAYLNKLGLSW
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCHH
AAASQDYDAILFGAKRLVRNLTITGKRKLPNKDVYVEIKPELIETEILLKKLGITREQLI
HHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHH
DIGILIGTDYNPDGIRGIGPERALKIIKKYGKIEKAMEYGEISKKDINFNIDEIRGLFLN
HEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEECC
PQVVKPEEALDLNEPNGEDIINILVYEHNFSEERVKNGIERLTKAIKEAKGASRQTGLDR
CCEECCHHHCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCC
WF
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726