Click here to switch to the map view.

The map label for this gene is pheS

Identifier: 15897061

GI number: 15897061

Start: 81106

End: 82512

Strand: Direct

Name: pheS

Synonym: SSO0100

Alternate gene names: 15897061

Gene position: 81106-82512 (Clockwise)

Preceding gene: 15897060

Following gene: 15897062

Centisome position: 2.71

GC content: 32.84

Gene sequence:

>1407_bases
GTGATATCAATGTTAAGTGAGAACGAAGCTAAGATACTATTTTTTTTGAAAGATCTTAAAAAAACAAATTCAGTGGAACT
TGCAATAAAAATGGGCATTCCAGAAAGCTCGGTCTTAAGTTTAATTGAGTTACTAAGGGAAAAGGGTTATGTAAAAACAG
AGGTAAAATCTGAAAAACATTATGTATTAACTGAAGAGGGGAGGAAAAGAAAAGAAAGTGGACTCCCAGAAGATATTTTA
ATAAATACGTTGAATGGACAAGAAAAAGATCTAAACGAGATAAAAAACATTCTTAATAAGGACTTTAACATCGCAATTAG
TTGGGCAAAGAGAAAAGGATTAATAGATATAAAAGAAGGAAAAGTGATACCTAAAGTAAAAACTTATATGTCATCTGAAT
ATCTTGCTCTTTCAAATCTTGAAAGAGCTGATAGTAATACAATAAATTTGCTTAAAAAAAGAGGTCTTATAGAAGAAAAG
GAAAGGAAAATTGTAAGTGTAGAGTTAATAAAAGAACCTAAAGAGTCTGAGATAGGTATTTCAAATCTGAATAGAGAATT
GATAATTAGTGGTGAGTGGAAGAAGTACAAGCTTAAGAAGTACAATGTAGAAGCCTTTCCTCCATATTATACTATTAGTA
AAAAACATTATTTTAGAGAGTTTTTAGAAAAAGTCAAGGATATTATGATTAGCTTAGGATTTAAAGAGATTAATACAGGG
TACATTGAAATGGAATTTTATAATTTTGATCTTTTGTTTCAACCTCAAGACCACCCTGCCAGAGAGATTCACGATAGCTT
TTCAGTAGAAGGTTCAGGAAAAATAGAAGACAAAGATTTGCTAAATAATGTAAAGGAAATTCATGAGAAATTCTGGAAGT
ATGAATGGAAACAAGATATTACACTAAGGCTAATGCTGAGAAGCCAGACTACTGCTACAACTGCGAGAGTCCTAGCATCA
AGACCTAAGGCTCCTCAAAAAGTCTTCACTTTAGGTAAGGTCTTCAGACCAGACGCAATAGATGCAACTCATTTGATAGA
ATTTCACCAATTAGATGGTGTAATTATAGACAATAATTTCACGTTTAAGGAGTTATTAGGTGTTCTGAAGGAAATATTCT
ATAGGTTAGGAATTAAGGAAGTTAAATTTAAACCAGCATACTTCCCCTTCACGGAACCTAGCGTAGAAGCTTACGGGTAT
CTGGAAAAATTAGGCTGGGTAGAGATGTGCGGGGCTGGACTGTTGAGACCAGAAATACTAAGTTCAGTTGGAATCGATAG
TACAGCTGGAGCTTGGGGAATTGGAATTGAAAGACTTGCTATGAGCTTTCTCAATATTAGTGATATTAGATTACTATATT
CTAACAATATAGAGTATATAAGAGATACTAAAGTGAAAATAGAGTGA

Upstream 100 bases:

>100_bases
CATGGGATCTGATAAAAGTTGACGTAAATGATTTTGTAAAACTAGTTAAGAACAAAGGATATAATCCCATAGTATTACAA
CCAGGCCAAACTATAACGTT

Downstream 100 bases:

>100_bases
TATAAATGGTAACTATAGTATTAAATAAATATAAATTATTAAATAAAATAGGCATAGGTCAACAGAAACTAGAGGATTTA
TTGTTTAACTTAAAATCAGA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MISMLSENEAKILFFLKDLKKTNSVELAIKMGIPESSVLSLIELLREKGYVKTEVKSEKHYVLTEEGRKRKESGLPEDIL
INTLNGQEKDLNEIKNILNKDFNIAISWAKRKGLIDIKEGKVIPKVKTYMSSEYLALSNLERADSNTINLLKKRGLIEEK
ERKIVSVELIKEPKESEIGISNLNRELIISGEWKKYKLKKYNVEAFPPYYTISKKHYFREFLEKVKDIMISLGFKEINTG
YIEMEFYNFDLLFQPQDHPAREIHDSFSVEGSGKIEDKDLLNNVKEIHEKFWKYEWKQDITLRLMLRSQTTATTARVLAS
RPKAPQKVFTLGKVFRPDAIDATHLIEFHQLDGVIIDNNFTFKELLGVLKEIFYRLGIKEVKFKPAYFPFTEPSVEAYGY
LEKLGWVEMCGAGLLRPEILSSVGIDSTAGAWGIGIERLAMSFLNISDIRLLYSNNIEYIRDTKVKIE

Sequences:

>Translated_468_residues
MISMLSENEAKILFFLKDLKKTNSVELAIKMGIPESSVLSLIELLREKGYVKTEVKSEKHYVLTEEGRKRKESGLPEDIL
INTLNGQEKDLNEIKNILNKDFNIAISWAKRKGLIDIKEGKVIPKVKTYMSSEYLALSNLERADSNTINLLKKRGLIEEK
ERKIVSVELIKEPKESEIGISNLNRELIISGEWKKYKLKKYNVEAFPPYYTISKKHYFREFLEKVKDIMISLGFKEINTG
YIEMEFYNFDLLFQPQDHPAREIHDSFSVEGSGKIEDKDLLNNVKEIHEKFWKYEWKQDITLRLMLRSQTTATTARVLAS
RPKAPQKVFTLGKVFRPDAIDATHLIEFHQLDGVIIDNNFTFKELLGVLKEIFYRLGIKEVKFKPAYFPFTEPSVEAYGY
LEKLGWVEMCGAGLLRPEILSSVGIDSTAGAWGIGIERLAMSFLNISDIRLLYSNNIEYIRDTKVKIE
>Mature_468_residues
MISMLSENEAKILFFLKDLKKTNSVELAIKMGIPESSVLSLIELLREKGYVKTEVKSEKHYVLTEEGRKRKESGLPEDIL
INTLNGQEKDLNEIKNILNKDFNIAISWAKRKGLIDIKEGKVIPKVKTYMSSEYLALSNLERADSNTINLLKKRGLIEEK
ERKIVSVELIKEPKESEIGISNLNRELIISGEWKKYKLKKYNVEAFPPYYTISKKHYFREFLEKVKDIMISLGFKEINTG
YIEMEFYNFDLLFQPQDHPAREIHDSFSVEGSGKIEDKDLLNNVKEIHEKFWKYEWKQDITLRLMLRSQTTATTARVLAS
RPKAPQKVFTLGKVFRPDAIDATHLIEFHQLDGVIIDNNFTFKELLGVLKEIFYRLGIKEVKFKPAYFPFTEPSVEAYGY
LEKLGWVEMCGAGLLRPEILSSVGIDSTAGAWGIGIERLAMSFLNISDIRLLYSNNIEYIRDTKVKIE

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=482, Percent_Identity=31.9502074688797, Blast_Score=223, Evalue=4e-58,
Organism=Homo sapiens, GI5729820, Length=297, Percent_Identity=24.9158249158249, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788007, Length=255, Percent_Identity=31.7647058823529, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32563657, Length=479, Percent_Identity=31.9415448851775, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17508957, Length=479, Percent_Identity=31.9415448851775, Blast_Score=229, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6321087, Length=339, Percent_Identity=35.1032448377581, Blast_Score=212, Evalue=1e-55,
Organism=Drosophila melanogaster, GI18858079, Length=478, Percent_Identity=30.7531380753138, Blast_Score=212, Evalue=6e-55,
Organism=Drosophila melanogaster, GI17137424, Length=267, Percent_Identity=25.8426966292135, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_SULSO (P95961)

Other databases:

- EMBL:   Y08257
- EMBL:   AE006641
- PIR:   S75389
- RefSeq:   NP_341666.1
- ProteinModelPortal:   P95961
- SMR:   P95961
- GeneID:   1453284
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0100
- NMPDR:   fig|273057.1.peg.89
- HOGENOM:   HBG497668
- OMA:   PEFYQCE
- PhylomeDB:   P95961
- ProtClustDB:   PRK04172
- BioCyc:   SSOL273057:SSO0100-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00282
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR022917
- InterPro:   IPR002319
- InterPro:   IPR002831
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF01978 TrmB; PF01409 tRNA-synt_2d

EC number: =6.1.1.20

Molecular weight: Translated: 54102; Mature: 54102

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISMLSENEAKILFFLKDLKKTNSVELAIKMGIPESSVLSLIELLREKGYVKTEVKSEKH
CCCCCCCCCCEEEEEEEHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCC
YVLTEEGRKRKESGLPEDILINTLNGQEKDLNEIKNILNKDFNIAISWAKRKGLIDIKEG
EEECCCCCHHHHCCCCHHHEEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCEEECCC
KVIPKVKTYMSSEYLALSNLERADSNTINLLKKRGLIEEKERKIVSVELIKEPKESEIGI
CCCHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHCCCCCHHHCCEEEEEECCCCCCCCCCH
SNLNRELIISGEWKKYKLKKYNVEAFPPYYTISKKHYFREFLEKVKDIMISLGFKEINTG
HCCCCEEEEECCCCEEEEEEECCEECCCEEEECHHHHHHHHHHHHHHHHHHCCCEECCCC
YIEMEFYNFDLLFQPQDHPAREIHDSFSVEGSGKIEDKDLLNNVKEIHEKFWKYEWKQDI
EEEEEEEEEEEEECCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
TLRLMLRSQTTATTARVLASRPKAPQKVFTLGKVFRPDAIDATHLIEFHQLDGVIIDNNF
EEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHECCCCEEEECCC
TFKELLGVLKEIFYRLGIKEVKFKPAYFPFTEPSVEAYGYLEKLGWVEMCGAGLLRPEIL
CHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCHHHHHHHHHCCHHHHHCCCCCCHHHH
SSVGIDSTAGAWGIGIERLAMSFLNISDIRLLYSNNIEYIRDTKVKIE
HHCCCCCCCCCHHHHHHHHHHHHHCHHHEEEEECCCCCEEECCEEEEC
>Mature Secondary Structure
MISMLSENEAKILFFLKDLKKTNSVELAIKMGIPESSVLSLIELLREKGYVKTEVKSEKH
CCCCCCCCCCEEEEEEEHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCC
YVLTEEGRKRKESGLPEDILINTLNGQEKDLNEIKNILNKDFNIAISWAKRKGLIDIKEG
EEECCCCCHHHHCCCCHHHEEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCEEECCC
KVIPKVKTYMSSEYLALSNLERADSNTINLLKKRGLIEEKERKIVSVELIKEPKESEIGI
CCCHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHCCCCCHHHCCEEEEEECCCCCCCCCCH
SNLNRELIISGEWKKYKLKKYNVEAFPPYYTISKKHYFREFLEKVKDIMISLGFKEINTG
HCCCCEEEEECCCCEEEEEEECCEECCCEEEECHHHHHHHHHHHHHHHHHHCCCEECCCC
YIEMEFYNFDLLFQPQDHPAREIHDSFSVEGSGKIEDKDLLNNVKEIHEKFWKYEWKQDI
EEEEEEEEEEEEECCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
TLRLMLRSQTTATTARVLASRPKAPQKVFTLGKVFRPDAIDATHLIEFHQLDGVIIDNNF
EEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHECCCCEEEECCC
TFKELLGVLKEIFYRLGIKEVKFKPAYFPFTEPSVEAYGYLEKLGWVEMCGAGLLRPEIL
CHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCHHHHHHHHHCCHHHHHCCCCCCHHHH
SSVGIDSTAGAWGIGIERLAMSFLNISDIRLLYSNNIEYIRDTKVKIE
HHCCCCCCCCCHHHHHHHHHHHHHCHHHEEEEECCCCCEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8899719; 11427726