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The map label for this gene is dnaG

Identifier: 15893253

GI number: 15893253

Start: 1233961

End: 1235748

Strand: Reverse

Name: dnaG

Synonym: RC1330

Alternate gene names: 15893253

Gene position: 1235748-1233961 (Counterclockwise)

Preceding gene: 15893254

Following gene: 15893252

Centisome position: 97.4

GC content: 30.65

Gene sequence:

>1788_bases
ATGAGAGTTGCACCGGAATTTTATGAGTTCTTAAGAAACCGTATTAACATATCGGATGTAGTGCGGCAAAAAGTAGCTTT
AACTAGAAAATCCGGTAACTATGTAGGTTTATGTCCCTTCCATCAAGAAAAGACCCCATCTTTTACGGTTAGTAATAGTA
AGAGATTTTTCTATTGTTTTGGTTGTAAAGCAGCCGGTGACGTTATAAAATTTACTTCTAATATTAGCGGTTTATCTTAT
AATGAGTCTGCTATAAAGCTTGCAATCGATTACGGTATAGAGATACCGAAATTAACTGCAAAACAGAAAGAATTTTATGA
AGAATCCGATGAAATTCTCAATATTTTAGAACTCGCCAATAAGTTTTTCAGGACACAATTAACACCTGAAATATTACATT
ATCTACACGAAAGGGGTATTACCGAAGAAACAGTAAAAGAGTTTTCAATAGGTTTTGCCCCGAAGAATAATAAATTTGAA
AAATTTTTTCACGATAAAAATATAGATATTATAAAGCTTGGTAAAGCAGGGCTAATCGGCAAGCGAGAAAACGGTGAAAT
ATATAATTTATTTTCTAACCGTATTACTATTCCAATTAGAAATATTTACAATAAAATTGTAGGCTTTGGCGGTAGAGTAC
TAGGGGAGGGGCTGCCTAAATACTTAAATTCACCTGAAACTACAGTATTTCAAAAAAGTGAAACTCTATACGGTGAGCAT
AAAGCGATAAGCAGTAGCTATAAAAAAAATCATTCGATTTTAGTAGAGGGTTATTTTGATGTTATAGCACTACATCAAGC
AGGTTTTAGTGAAACGGTTGCGAGTCTCGGTACTAGTGTTACTGAGAATCACTTACATAAATTATGGCGTGCTGGCGATG
AAATTATATTATGTCTTGACGGAGATAATGCAGGCATAAAAGCTAGTATAAGAACTATTAACTTAGCATTACCGCTTATT
AATAGTGAAAAGAAAATTTCTTTTATTAGGTTACCAAGCGGACTTGATCCCGACGATGCAGTAAATAAAAACGGTGCTGA
TTTTTTTGCAAAGCTTATAGATAAAAGAATAAGCTTATCAGAAATGATTTGGCATATTGAGTATAGCGGCAAGAGTTTTA
AAACTGCGGAAGAAAAAGCTAATTTAGAAAAAAATTTAAAAAATTATTGCAGCAAAATATCGGATAGTGATTTAAAAGTA
AGTTATTACAGGTTTTTTAAAGATCAAATATGGCAAAATTTAGTTACAACGCAGAAGAAAGCTACAACTAAAAACTCAAA
TTTGGCTCCTATAATTTCAAGTCACGGCTATTCGGAGCTTGAAATACTAGAACACGCTTTTTGTGCTTTGTTGGTTAAAT
TTCCTATAATACTTGAAGAAAAAGATATTAGAGATTTCATCTTAAATTTAAATTTTAATAATAAATCGCTTGAAGAATTC
CGTAATTGGTATTTAAACGAGATTATAGATAATAACGTAGAAGCAAGTGAAATTACTGCAATTGTGGAAAAAACTAGCTT
TTTTGATATTTTTTTATTATTATCAAAAACGGATAATTTGTTTTTAGATATCTCATTTAATAAAAATAATATCAGACTGG
ATTTATTATGGCAGTGGCTACATAAGAAATATTATCTAATAAATTTACAACAAGAATACGCTATTACCATAAACAGTACT
GACAATCACGACTTTGAAAAAGTTTTACTATATAAAAAAGAAATTCTGAAGATTGCAAATGAACTGCAAGTTTTAAACGA
ATCTTTTATTAATCACACGATAACTTAA

Upstream 100 bases:

>100_bases
GCTGAATTTTATTCAAAAGATTTAAGAATTAACGATAATTTGTCTATACCGCTTTCTTATTGTCTTTCTACAGCTATTCT
TTCCTACATATTGTAATATA

Downstream 100 bases:

>100_bases
TTATTTCAAACTTCTTTTAAAAGAAAAAAGCATATAGGTATTTAATGAGCAATAGCAATATAGACAATGATCTAGATAAA
ATAGATAGTTTACTTAAAAA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MRVAPEFYEFLRNRINISDVVRQKVALTRKSGNYVGLCPFHQEKTPSFTVSNSKRFFYCFGCKAAGDVIKFTSNISGLSY
NESAIKLAIDYGIEIPKLTAKQKEFYEESDEILNILELANKFFRTQLTPEILHYLHERGITEETVKEFSIGFAPKNNKFE
KFFHDKNIDIIKLGKAGLIGKRENGEIYNLFSNRITIPIRNIYNKIVGFGGRVLGEGLPKYLNSPETTVFQKSETLYGEH
KAISSSYKKNHSILVEGYFDVIALHQAGFSETVASLGTSVTENHLHKLWRAGDEIILCLDGDNAGIKASIRTINLALPLI
NSEKKISFIRLPSGLDPDDAVNKNGADFFAKLIDKRISLSEMIWHIEYSGKSFKTAEEKANLEKNLKNYCSKISDSDLKV
SYYRFFKDQIWQNLVTTQKKATTKNSNLAPIISSHGYSELEILEHAFCALLVKFPIILEEKDIRDFILNLNFNNKSLEEF
RNWYLNEIIDNNVEASEITAIVEKTSFFDIFLLLSKTDNLFLDISFNKNNIRLDLLWQWLHKKYYLINLQQEYAITINST
DNHDFEKVLLYKKEILKIANELQVLNESFINHTIT

Sequences:

>Translated_595_residues
MRVAPEFYEFLRNRINISDVVRQKVALTRKSGNYVGLCPFHQEKTPSFTVSNSKRFFYCFGCKAAGDVIKFTSNISGLSY
NESAIKLAIDYGIEIPKLTAKQKEFYEESDEILNILELANKFFRTQLTPEILHYLHERGITEETVKEFSIGFAPKNNKFE
KFFHDKNIDIIKLGKAGLIGKRENGEIYNLFSNRITIPIRNIYNKIVGFGGRVLGEGLPKYLNSPETTVFQKSETLYGEH
KAISSSYKKNHSILVEGYFDVIALHQAGFSETVASLGTSVTENHLHKLWRAGDEIILCLDGDNAGIKASIRTINLALPLI
NSEKKISFIRLPSGLDPDDAVNKNGADFFAKLIDKRISLSEMIWHIEYSGKSFKTAEEKANLEKNLKNYCSKISDSDLKV
SYYRFFKDQIWQNLVTTQKKATTKNSNLAPIISSHGYSELEILEHAFCALLVKFPIILEEKDIRDFILNLNFNNKSLEEF
RNWYLNEIIDNNVEASEITAIVEKTSFFDIFLLLSKTDNLFLDISFNKNNIRLDLLWQWLHKKYYLINLQQEYAITINST
DNHDFEKVLLYKKEILKIANELQVLNESFINHTIT
>Mature_595_residues
MRVAPEFYEFLRNRINISDVVRQKVALTRKSGNYVGLCPFHQEKTPSFTVSNSKRFFYCFGCKAAGDVIKFTSNISGLSY
NESAIKLAIDYGIEIPKLTAKQKEFYEESDEILNILELANKFFRTQLTPEILHYLHERGITEETVKEFSIGFAPKNNKFE
KFFHDKNIDIIKLGKAGLIGKRENGEIYNLFSNRITIPIRNIYNKIVGFGGRVLGEGLPKYLNSPETTVFQKSETLYGEH
KAISSSYKKNHSILVEGYFDVIALHQAGFSETVASLGTSVTENHLHKLWRAGDEIILCLDGDNAGIKASIRTINLALPLI
NSEKKISFIRLPSGLDPDDAVNKNGADFFAKLIDKRISLSEMIWHIEYSGKSFKTAEEKANLEKNLKNYCSKISDSDLKV
SYYRFFKDQIWQNLVTTQKKATTKNSNLAPIISSHGYSELEILEHAFCALLVKFPIILEEKDIRDFILNLNFNNKSLEEF
RNWYLNEIIDNNVEASEITAIVEKTSFFDIFLLLSKTDNLFLDISFNKNNIRLDLLWQWLHKKYYLINLQQEYAITINST
DNHDFEKVLLYKKEILKIANELQVLNESFINHTIT

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Escherichia coli, GI1789447, Length=406, Percent_Identity=34.7290640394089, Blast_Score=266, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIM_RICCN (Q92FZ7)

Other databases:

- EMBL:   AE006914
- PIR:   B97866
- RefSeq:   NP_360967.1
- HSSP:   Q9X4D0
- ProteinModelPortal:   Q92FZ7
- SMR:   Q92FZ7
- GeneID:   928481
- GenomeReviews:   AE006914_GR
- KEGG:   rco:RC1330
- NMPDR:   fig|272944.1.peg.1330
- OMA:   TREQTLN
- ProtClustDB:   PRK05667
- BioCyc:   RCON272944:RC1330-MONOMER
- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694
- Gene3D:   G3DSA:3.90.980.10
- Gene3D:   G3DSA:3.90.580.10
- SMART:   SM00493
- SMART:   SM00400
- TIGRFAMs:   TIGR01391

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2

EC number: 2.7.7.-

Molecular weight: Translated: 68424; Mature: 68424

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVAPEFYEFLRNRINISDVVRQKVALTRKSGNYVGLCPFHQEKTPSFTVSNSKRFFYCF
CCCCHHHHHHHHCCCCHHHHHHHHHHHEECCCCEEEECCCCCCCCCCEEEECCCEEEEEE
GCKAAGDVIKFTSNISGLSYNESAIKLAIDYGIEIPKLTAKQKEFYEESDEILNILELAN
CCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KFFRTQLTPEILHYLHERGITEETVKEFSIGFAPKNNKFEKFFHDKNIDIIKLGKAGLIG
HHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCEEEECCCCCCC
KRENGEIYNLFSNRITIPIRNIYNKIVGFGGRVLGEGLPKYLNSPETTVFQKSETLYGEH
CCCCCCEEEEECCEEEEEHHHHHHHHHCCCCHHHHCCCHHHHCCCCCEEEECCCHHCCHH
KAISSSYKKNHSILVEGYFDVIALHQAGFSETVASLGTSVTENHLHKLWRAGDEIILCLD
HHHHHHHCCCCCEEEEEHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEC
GDNAGIKASIRTINLALPLINSEKKISFIRLPSGLDPDDAVNKNGADFFAKLIDKRISLS
CCCCCEEEEEEEEEEEEEECCCCCCEEEEECCCCCCCCHHCCCCCHHHHHHHHHHHCCHH
EMIWHIEYSGKSFKTAEEKANLEKNLKNYCSKISDSDLKVSYYRFFKDQIWQNLVTTQKK
HEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ATTKNSNLAPIISSHGYSELEILEHAFCALLVKFPIILEEKDIRDFILNLNFNNKSLEEF
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEEECCCCCHHHHH
RNWYLNEIIDNNVEASEITAIVEKTSFFDIFLLLSKTDNLFLDISFNKNNIRLDLLWQWL
HHHHHHHHHCCCCCHHHHHHHHHHCHHEEEEEEEECCCCEEEEEEECCCCEEHHHHHHHH
HKKYYLINLQQEYAITINSTDNHDFEKVLLYKKEILKIANELQVLNESFINHTIT
CCEEEEEEEECEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRVAPEFYEFLRNRINISDVVRQKVALTRKSGNYVGLCPFHQEKTPSFTVSNSKRFFYCF
CCCCHHHHHHHHCCCCHHHHHHHHHHHEECCCCEEEECCCCCCCCCCEEEECCCEEEEEE
GCKAAGDVIKFTSNISGLSYNESAIKLAIDYGIEIPKLTAKQKEFYEESDEILNILELAN
CCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KFFRTQLTPEILHYLHERGITEETVKEFSIGFAPKNNKFEKFFHDKNIDIIKLGKAGLIG
HHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCEEEECCCCCCC
KRENGEIYNLFSNRITIPIRNIYNKIVGFGGRVLGEGLPKYLNSPETTVFQKSETLYGEH
CCCCCCEEEEECCEEEEEHHHHHHHHHCCCCHHHHCCCHHHHCCCCCEEEECCCHHCCHH
KAISSSYKKNHSILVEGYFDVIALHQAGFSETVASLGTSVTENHLHKLWRAGDEIILCLD
HHHHHHHCCCCCEEEEEHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEC
GDNAGIKASIRTINLALPLINSEKKISFIRLPSGLDPDDAVNKNGADFFAKLIDKRISLS
CCCCCEEEEEEEEEEEEEECCCCCCEEEEECCCCCCCCHHCCCCCHHHHHHHHHHHCCHH
EMIWHIEYSGKSFKTAEEKANLEKNLKNYCSKISDSDLKVSYYRFFKDQIWQNLVTTQKK
HEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ATTKNSNLAPIISSHGYSELEILEHAFCALLVKFPIILEEKDIRDFILNLNFNNKSLEEF
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEEECCCCCHHHHH
RNWYLNEIIDNNVEASEITAIVEKTSFFDIFLLLSKTDNLFLDISFNKNNIRLDLLWQWL
HHHHHHHHHCCCCCHHHHHHHHHHCHHEEEEEEEECCCCEEEEEEECCCCEEHHHHHHHH
HKKYYLINLQQEYAITINSTDNHDFEKVLLYKKEILKIANELQVLNESFINHTIT
CCEEEEEEEECEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893