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The map label for this gene is truA [H]

Identifier: 15893251

GI number: 15893251

Start: 1231179

End: 1231916

Strand: Reverse

Name: truA [H]

Synonym: RC1328

Alternate gene names: 15893251

Gene position: 1231916-1231179 (Counterclockwise)

Preceding gene: 15893252

Following gene: 15893249

Centisome position: 97.1

GC content: 36.04

Gene sequence:

>738_bases
ATGTATCGATATAAAATTACCATTGAATATTTAGGCACGGATCTTGCAGGTTGGCAAAGACAAGCAGGAGTAATGTCGGT
ACAGCAGATATTAGAAGAAGCGATTTATAAATTTTCCGGTGAACAGGTGATATTGTTTGGGTCAGGAAGGACAGATGCCG
GTGTTCATGCTATAGGTCAAGTAGCACATTTTGATCTTTCAAAATATTTAGAACCTCACAAAATTATTACAGCTATCAAC
TATTTTGCAAGACCGTATGCCGTAGGAGTATGGAATTGTGAATTAGCCCCCAATAATTTTCATGCTAGGTTTTCGGCTAC
TTCTCGTTATTATATATATAGAATTATTAATAGACCTTATCCATCGGTAATTGATTTAAATAGAGCATGGTGGATTAGTG
CACCTTTAGATGTTCCGGCTATGCAACAAGCTGCTGTCTATCTATTAGGCAAACATGATTTTACTTCATTTAGGGCTAGC
TCATGTCAATCAAAATCACCGATAAAAACTTTAACTGAACTTAATATTATTAAAGAAGACGAAGAAATAAAATTATATCT
TTCAGCCCCGTCATTTTTGCATCATATGGTACGTAATATTGTCGGCAGTTTAGTACTTGTTGGTAAAAATATTTGGCAGG
CAGAACAAATTAAAGATGTTTTAGAAGCCAAAGATAGAAAAGCCGCTGGTCCTACTGCTCCCGCCTCCGGTCTTTATTTT
GTGAAAACAGCGTATTAA

Upstream 100 bases:

>100_bases
CATGATAATACTTATACTATCATCCCGTGATTTGGGATCTCAGGACACGGGCTGTGATACAAGATCCCGTGGTCAAGCCA
CGGGATGACAGTGGGCTATT

Downstream 100 bases:

>100_bases
CAACCATAAATTTTGTCATTACAATGCCTCCCTGCATAGGCATTGTTATGTGGATACCACCTTGTCATTGCGAGGAAATT
ACGAAGTAATTGACGAAGCA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MYRYKITIEYLGTDLAGWQRQAGVMSVQQILEEAIYKFSGEQVILFGSGRTDAGVHAIGQVAHFDLSKYLEPHKIITAIN
YFARPYAVGVWNCELAPNNFHARFSATSRYYIYRIINRPYPSVIDLNRAWWISAPLDVPAMQQAAVYLLGKHDFTSFRAS
SCQSKSPIKTLTELNIIKEDEEIKLYLSAPSFLHHMVRNIVGSLVLVGKNIWQAEQIKDVLEAKDRKAAGPTAPASGLYF
VKTAY

Sequences:

>Translated_245_residues
MYRYKITIEYLGTDLAGWQRQAGVMSVQQILEEAIYKFSGEQVILFGSGRTDAGVHAIGQVAHFDLSKYLEPHKIITAIN
YFARPYAVGVWNCELAPNNFHARFSATSRYYIYRIINRPYPSVIDLNRAWWISAPLDVPAMQQAAVYLLGKHDFTSFRAS
SCQSKSPIKTLTELNIIKEDEEIKLYLSAPSFLHHMVRNIVGSLVLVGKNIWQAEQIKDVLEAKDRKAAGPTAPASGLYF
VKTAY
>Mature_245_residues
MYRYKITIEYLGTDLAGWQRQAGVMSVQQILEEAIYKFSGEQVILFGSGRTDAGVHAIGQVAHFDLSKYLEPHKIITAIN
YFARPYAVGVWNCELAPNNFHARFSATSRYYIYRIINRPYPSVIDLNRAWWISAPLDVPAMQQAAVYLLGKHDFTSFRAS
SCQSKSPIKTLTELNIIKEDEEIKLYLSAPSFLHHMVRNIVGSLVLVGKNIWQAEQIKDVLEAKDRKAAGPTAPASGLYF
VKTAY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=275, Percent_Identity=34.1818181818182, Blast_Score=133, Evalue=1e-31,
Organism=Escherichia coli, GI1788657, Length=246, Percent_Identity=41.869918699187, Blast_Score=182, Evalue=2e-47,
Organism=Drosophila melanogaster, GI116007752, Length=301, Percent_Identity=26.578073089701, Blast_Score=91, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27682; Mature: 27682

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRYKITIEYLGTDLAGWQRQAGVMSVQQILEEAIYKFSGEQVILFGSGRTDAGVHAIGQ
CEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHH
VAHFDLSKYLEPHKIITAINYFARPYAVGVWNCELAPNNFHARFSATSRYYIYRIINRPY
HHHHHHHHHCCHHHHHHHHHHHHCCEEEEEEEEEECCCCCEEEEECCCCEEEEEEECCCC
PSVIDLNRAWWISAPLDVPAMQQAAVYLLGKHDFTSFRASSCQSKSPIKTLTELNIIKED
CCEEECCCEEEEECCCCCCHHHHHEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHEEECC
EEIKLYLSAPSFLHHMVRNIVGSLVLVGKNIWQAEQIKDVLEAKDRKAAGPTAPASGLYF
CEEEEEECCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEE
VKTAY
EEECC
>Mature Secondary Structure
MYRYKITIEYLGTDLAGWQRQAGVMSVQQILEEAIYKFSGEQVILFGSGRTDAGVHAIGQ
CEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHH
VAHFDLSKYLEPHKIITAINYFARPYAVGVWNCELAPNNFHARFSATSRYYIYRIINRPY
HHHHHHHHHCCHHHHHHHHHHHHCCEEEEEEEEEECCCCCEEEEECCCCEEEEEEECCCC
PSVIDLNRAWWISAPLDVPAMQQAAVYLLGKHDFTSFRASSCQSKSPIKTLTELNIIKED
CCEEECCCEEEEECCCCCCHHHHHEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHEEECC
EEIKLYLSAPSFLHHMVRNIVGSLVLVGKNIWQAEQIKDVLEAKDRKAAGPTAPASGLYF
CEEEEEECCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEE
VKTAY
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA