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The map label for this gene is proP [H]

Identifier: 15893245

GI number: 15893245

Start: 1224109

End: 1224567

Strand: Reverse

Name: proP [H]

Synonym: RC1322

Alternate gene names: 15893245

Gene position: 1224567-1224109 (Counterclockwise)

Preceding gene: 15893246

Following gene: 15893244

Centisome position: 96.52

GC content: 40.09

Gene sequence:

>459_bases
ATGCAAGAGACGTTAACTTTTGCGGTATTTGCTGCAGGCTTTGTAGTACGACCGCTCGGCGGTATTATTTTCGGTAATAT
AGGTGATAGATTCGGTAGACGTAGTTATAAAACTATTGGAATTGCCGCTCCTATTATTTTAACTATTATCAGACTCATAC
AAGGTTTTTCTTTAGGAGGGGAATTTAGCGGTTGTATTTCGTATATTGTAGAACATGCTTCGTTCGAGCAGCGAGGACTT
GCGGGGAGTAGCTCATGTGTTAGTATGTGCGGCGGTATGCTGCTTGGACTTGGTACAGCAGCTGGTTTTTCTTATTTTAT
GCCTGCCGATATTCTATTTGAATGGGGTTGGAGGATACCTTTTATTGCCGGTTTATTTATTAGCTCCGTAGGTTTATATA
TTAGAAAAAACCTAGCAGAAAGTCCTATTTATAAAAAAGCTAAGGGAACAAGGCGTTGA

Upstream 100 bases:

>100_bases
GTAATATCCGGCATGATCGGCAACGCTTTAGAATGGTATGATTATGCATTATATGCGCAATTTGCTTATATTATAGGGCA
GCATTTTTCCGGATCCAGAA

Downstream 100 bases:

>100_bases
GCACGTTTTCCTTTACGAGAAACATTAACAAAATACCCAAAAGAACTAATTATCGCACTTGGTCTGTATATTACCGTAAC
TGCTTCTTTTTATACTTCTA

Product: putative proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MQETLTFAVFAAGFVVRPLGGIIFGNIGDRFGRRSYKTIGIAAPIILTIIRLIQGFSLGGEFSGCISYIVEHASFEQRGL
AGSSSCVSMCGGMLLGLGTAAGFSYFMPADILFEWGWRIPFIAGLFISSVGLYIRKNLAESPIYKKAKGTRR

Sequences:

>Translated_152_residues
MQETLTFAVFAAGFVVRPLGGIIFGNIGDRFGRRSYKTIGIAAPIILTIIRLIQGFSLGGEFSGCISYIVEHASFEQRGL
AGSSSCVSMCGGMLLGLGTAAGFSYFMPADILFEWGWRIPFIAGLFISSVGLYIRKNLAESPIYKKAKGTRR
>Mature_152_residues
MQETLTFAVFAAGFVVRPLGGIIFGNIGDRFGRRSYKTIGIAAPIILTIIRLIQGFSLGGEFSGCISYIVEHASFEQRGL
AGSSSCVSMCGGMLLGLGTAAGFSYFMPADILFEWGWRIPFIAGLFISSVGLYIRKNLAESPIYKKAKGTRR

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=167, Percent_Identity=35.3293413173653, Blast_Score=110, Evalue=5e-26,
Organism=Escherichia coli, GI1788292, Length=163, Percent_Identity=34.3558282208589, Blast_Score=96, Evalue=7e-22,
Organism=Escherichia coli, GI1788942, Length=161, Percent_Identity=37.2670807453416, Blast_Score=84, Evalue=3e-18,
Organism=Escherichia coli, GI1789941, Length=161, Percent_Identity=34.7826086956522, Blast_Score=81, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 16418; Mature: 16418

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQETLTFAVFAAGFVVRPLGGIIFGNIGDRFGRRSYKTIGIAAPIILTIIRLIQGFSLGG
CCCHHHHHHHHHHHHHHHHHHEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
EFSGCISYIVEHASFEQRGLAGSSSCVSMCGGMLLGLGTAAGFSYFMPADILFEWGWRIP
CHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHCHHHHCHHHHHHHCCCCHH
FIAGLFISSVGLYIRKNLAESPIYKKAKGTRR
HHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCC
>Mature Secondary Structure
MQETLTFAVFAAGFVVRPLGGIIFGNIGDRFGRRSYKTIGIAAPIILTIIRLIQGFSLGG
CCCHHHHHHHHHHHHHHHHHHEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
EFSGCISYIVEHASFEQRGLAGSSSCVSMCGGMLLGLGTAAGFSYFMPADILFEWGWRIP
CHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHCHHHHCHHHHHHHCCCCHH
FIAGLFISSVGLYIRKNLAESPIYKKAKGTRR
HHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]