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The map label for this gene is proP5 [H]

Identifier: 15893093

GI number: 15893093

Start: 1070474

End: 1071781

Strand: Reverse

Name: proP5 [H]

Synonym: RC1170

Alternate gene names: 15893093

Gene position: 1071781-1070474 (Counterclockwise)

Preceding gene: 15893094

Following gene: 15893092

Centisome position: 84.48

GC content: 33.49

Gene sequence:

>1308_bases
TTGCTCTTTTCCAAATTAAACTTCGTCGTCTACCTACTTTTCGTTTACTCACAGTATACCTTTAAAATGAAACAAACAAA
ATCACGCAATAAAGTTCTCGGTGCTTTTTTAGGCACTATTATTGAATATTACGACTATAGTTTATATGGTTTTTCAGCGG
CAATTATCGCTGATAAATTCTTTTCAGCCAGTACTGATCTATTAACAAAGCTTGTAAATGTTTTTGCCGTTTATGCAGCA
GCATATTTATCAAAGCCTATGGGTGCTTATATTTTTGGACGTATAGGGGATATATACGGTCGAAAAAAAGCCTTAAGTTT
TACGATTATCGGTATTGTAATCCCTACCTTAATAATAGGTTTATTGCCCGATTATTCCTCGATAGGAGTTTGGAGTACAA
TAATTTTAGTTTTATGTCGCTTCATGCAAGGTATTTTTATTGGAGGGGAATATGACGGTGCAGCGATTTATGTTATTGAA
CATTTAGGAGCAAAATACCGATTTACTGCCTCGGCAATAACTAGATGTACAGGAGTAATGGGTTTATTATGCGGAATCGG
TGCAACTAATTTCTTTAATTCTCATATTTTTCCGGAGTGGGGTTGGCGTATTCCGTTTTTACTAAGTTTACCACTTGCAT
TAATAACTCTTTATTATCGCCGGAAATTTGATGAAACACCGGAGTTTAAAAAAGCACACTATAGCCAAGATTCTATTGAA
AAACTTAGCTCTATAATTAAAAAGCAATGGAAAAATATTGCACGGTTAATATTTTTAGCCGGAGGTTTTGGAGCAACATA
TCAAATTGCTATCATCTTTATGAAGCAATATTTACCGATCGTATTACCTTCAATCGGTATTATTATGAGTGCTTTTTCGG
TCTTAATAGTAATATGTTTTGCTGTTTGCATGCCGATTGCCGGTTTTATTGCCGATCGCTTAGGAGTTAATTCAGTATTA
AAAATCGCATTTATATGTACTATTACAGCAAGTGTATTTTTTGTGATAGCAGTAAAATATCAAATGACTAATTTAGGGCT
TGCAGCTAGTTTAATGTTAGCTGCATCAGTTGCACCTTTTAATTCTCTTGCTCATAGTATAGTTATTAAGTCTTTTGTAG
TTAAAGAACGCTATCGTGTTATAAGTTTAGGACATAATATCGGCTCAATGTTAATGTCCGGTACTGCTAATTATATTTGT
GCAAAGGTAATAAAATCATTTGATTTTAACCTATTCCCGATTTTATATCTTTGCATGTTTGCCGTACTTGCTTATTCTAT
GACCATATTATTTAACCGTAATAGGTAA

Upstream 100 bases:

>100_bases
TCATGTATACTCGTTTAATTTGAAAAATTGGCTACGTTGTCTTTGGCTGATAATCCTCACGTACTAGTATGTACACTGCG
GTTATCAGCTTCAAAACGCC

Downstream 100 bases:

>100_bases
AAAATAAATCTTGACGATAACTTTAAATGTTATGCATAAAATTTTTCATAAATCTATAATTTTTATTATCTTATGTATAC
AAGGAAATATTAGTTTTACC

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MLFSKLNFVVYLLFVYSQYTFKMKQTKSRNKVLGAFLGTIIEYYDYSLYGFSAAIIADKFFSASTDLLTKLVNVFAVYAA
AYLSKPMGAYIFGRIGDIYGRKKALSFTIIGIVIPTLIIGLLPDYSSIGVWSTIILVLCRFMQGIFIGGEYDGAAIYVIE
HLGAKYRFTASAITRCTGVMGLLCGIGATNFFNSHIFPEWGWRIPFLLSLPLALITLYYRRKFDETPEFKKAHYSQDSIE
KLSSIIKKQWKNIARLIFLAGGFGATYQIAIIFMKQYLPIVLPSIGIIMSAFSVLIVICFAVCMPIAGFIADRLGVNSVL
KIAFICTITASVFFVIAVKYQMTNLGLAASLMLAASVAPFNSLAHSIVIKSFVVKERYRVISLGHNIGSMLMSGTANYIC
AKVIKSFDFNLFPILYLCMFAVLAYSMTILFNRNR

Sequences:

>Translated_435_residues
MLFSKLNFVVYLLFVYSQYTFKMKQTKSRNKVLGAFLGTIIEYYDYSLYGFSAAIIADKFFSASTDLLTKLVNVFAVYAA
AYLSKPMGAYIFGRIGDIYGRKKALSFTIIGIVIPTLIIGLLPDYSSIGVWSTIILVLCRFMQGIFIGGEYDGAAIYVIE
HLGAKYRFTASAITRCTGVMGLLCGIGATNFFNSHIFPEWGWRIPFLLSLPLALITLYYRRKFDETPEFKKAHYSQDSIE
KLSSIIKKQWKNIARLIFLAGGFGATYQIAIIFMKQYLPIVLPSIGIIMSAFSVLIVICFAVCMPIAGFIADRLGVNSVL
KIAFICTITASVFFVIAVKYQMTNLGLAASLMLAASVAPFNSLAHSIVIKSFVVKERYRVISLGHNIGSMLMSGTANYIC
AKVIKSFDFNLFPILYLCMFAVLAYSMTILFNRNR
>Mature_435_residues
MLFSKLNFVVYLLFVYSQYTFKMKQTKSRNKVLGAFLGTIIEYYDYSLYGFSAAIIADKFFSASTDLLTKLVNVFAVYAA
AYLSKPMGAYIFGRIGDIYGRKKALSFTIIGIVIPTLIIGLLPDYSSIGVWSTIILVLCRFMQGIFIGGEYDGAAIYVIE
HLGAKYRFTASAITRCTGVMGLLCGIGATNFFNSHIFPEWGWRIPFLLSLPLALITLYYRRKFDETPEFKKAHYSQDSIE
KLSSIIKKQWKNIARLIFLAGGFGATYQIAIIFMKQYLPIVLPSIGIIMSAFSVLIVICFAVCMPIAGFIADRLGVNSVL
KIAFICTITASVFFVIAVKYQMTNLGLAASLMLAASVAPFNSLAHSIVIKSFVVKERYRVISLGHNIGSMLMSGTANYIC
AKVIKSFDFNLFPILYLCMFAVLAYSMTILFNRNR

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=420, Percent_Identity=29.7619047619048, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1788292, Length=436, Percent_Identity=28.8990825688073, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI1788942, Length=431, Percent_Identity=26.2180974477958, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789941, Length=431, Percent_Identity=26.9141531322506, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87082404, Length=350, Percent_Identity=24.8571428571429, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87082231, Length=215, Percent_Identity=25.5813953488372, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48524; Mature: 48524

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHEEECCC
>Mature Secondary Structure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HHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]