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The map label for this gene is ostA [H]

Identifier: 15892947

GI number: 15892947

Start: 950538

End: 952679

Strand: Reverse

Name: ostA [H]

Synonym: RC1024

Alternate gene names: 15892947

Gene position: 952679-950538 (Counterclockwise)

Preceding gene: 15892954

Following gene: 15892946

Centisome position: 75.09

GC content: 31.7

Gene sequence:

>2142_bases
ATGCTACTGCGAATAATATGTTTGCTTATTATCATAATATTTTCTTTGGTAAGTTTTGCCGCTCTTTCTAAGAATGGTCA
AAATATTAAAATAAGCAATATTATAGCTGACTTCGTAGAGTATAATGAAAATCAGGATTTGATATATGCTAAAGGTAATA
TAAGAATTATCACGGATGAGTATTTATTAACAGTAGATAATTTACTTTATGATATAGAAAACGATATTTTAAGGGCAGAA
GGTAATATCCGAATTAAGGATAAACAAAATCGGATAATAGAGGGAGACAAAGCGGTTTTAAAAGATGAATTTAAGAAAGG
TATCATATCGGAATTCATTTTGCTTTCCGGTGATAATAATCTTTTAATTGCTAAACTTGCCGAAAGAATAGATGAAAATA
ATCTTAAGCTGCACCATGCCGAGTTTACCCCTTGTGATGTTGCTTGTAATAGAAATCCTATTTGGCAAATTGCTGCTAAG
GATACTTATATTCATTCCGCAGAGCATAGAGTAGTTTATAAAAACGTGTTTTTTGAAGTATACGGTGTACCGATTTGTTA
CTTACCGTATTTTTTCCATCCGACTCATACGGCCCCTGCAACTTCTGGATTATTGGTACCTGACGTCAAAAATAAAGGGT
TTGGCATCCCCCTATATCTACGTGCAAAACCTAATATCGATTTTACTCTCACTCCAAGAATTTTTAGTAAATATCAAACT
TATGAGCTAGAAGCACGTTATCGCCTGAATGATACCGATAATATGAATTTTCAAGTTAGTTACGGTAAATTACCGTATTT
TCTAGAAAAAGATGGAAGTGTAGTGAAAAATAAAAAAGTTTCTTCTTATCATTATATAGCAAGCGGTAATTTTATTAGTA
CCGATAAAGTTTATGATTACGGTTTCAGGGTGGAGCGTACATCGGATAAAGCTTATTTAAAAAATTATTATAATAACTAC
GCTTCATATTTAACTTCTAAAATATTCTTAAATAAAATTCATGAGGCTGATTATTTTTCGGCGGAAGGATTAAGTTTTCA
AGGCTTAGGTAGTAATGATCGTAAATATACCGATCCTCTAGTATTACCAAAAATCAATACAAAAAACGTGATAAATTTGA
ATGATAATGACAACAGCCATATCGTTGTTGAAAATAATACGTTAATGTACAAAGAAAGAATAGGAAAACAAGTTGCTAGA
ACCTCTTTGCAACTATCCTTTATGCATAATGTGCTGACTGCTTCAGGACAAATTTTTGATTTTGCTGCTAAAGATAGAGG
CGATTTTTATCTTGTTAGGCGGGCTTATGTAGATAAGCCGAGAGAAAGTAAAGCATTTCAAAGGAATATACCGGAATTGC
AAACTTTATGGCGTTATCCGTTAGTAGGCAATATAACAAAAACGATAAATTTACTTATTGAGCCAGTAGTTTCTTTTACT
ATGGGACGTAAACTATCTAAGAAAGATAAAAAATTTGTTATAATTGATCCTGCTAAATATGAATTATCCGAAAAAAATAT
CTTTTTATCCAACCGTTATAGCGGTATTGATTGCTATGATTTCGGTAATAGATTAATTTACGGCTTAAATACTAGTATTG
CTAAAGAAGAAAATTATTTAAAATTATTTTTAGGACACTCCCGTAATACAAGATATAGTATAAATTTGCCAGCTGATGAT
AAGGAAAATGTAGGGCAGATACTAGGTAATCTTTCTAATAATTTAGAAGTGTTTTATAGCTTTCGGAAAGATAGATATTT
CAGACCTATTAGGGATGAAGTGGGAGGAAATTTTAACTGTGATAAGATTAACTTTCTAGGTAGTTTTATTCAGCTTACCA
ATCTTAACAAATATTATTCCGTAGAAAGTATAAAGGTTTCAAATAATAGAGTACGACAATTTTATGGAGACATAAATTAT
CAGCTAGCGGAAAATTGGACTATAGGCTTTGGTGCACGCATTGATCTCTCTAGACGGTCTCCAAATTTACTTACGCGAAC
TATTAGGATGACATATGCTAAAGATTGTGTTAGAATTACTGCAAAGATTTATTCCGATTATATAGCCGATGGAAATAGAG
GAATCAAAAAGACTACCTCTTCGCCGACTATTTCTATCGGTTTAAAAGTTTTAAACATGTGA

Upstream 100 bases:

>100_bases
ACCTTGTACTACAAAAGAGCAAGCAAAATTTTTTATTATTTGATATATTGCTAATAGCTTGCTATTATTTTTTACTATTA
AAATATATTTATTAATATAA

Downstream 100 bases:

>100_bases
GTATACGAAAAATTGGCTATGTCGTCTTATAAGTTCTGCGGTGCTCACGTACTAAATGTACGCTCCGCTCCTTGACTTAT
AGACTCCTTGCTCTTTTCCA

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 713; Mature: 713

Protein sequence:

>713_residues
MLLRIICLLIIIIFSLVSFAALSKNGQNIKISNIIADFVEYNENQDLIYAKGNIRIITDEYLLTVDNLLYDIENDILRAE
GNIRIKDKQNRIIEGDKAVLKDEFKKGIISEFILLSGDNNLLIAKLAERIDENNLKLHHAEFTPCDVACNRNPIWQIAAK
DTYIHSAEHRVVYKNVFFEVYGVPICYLPYFFHPTHTAPATSGLLVPDVKNKGFGIPLYLRAKPNIDFTLTPRIFSKYQT
YELEARYRLNDTDNMNFQVSYGKLPYFLEKDGSVVKNKKVSSYHYIASGNFISTDKVYDYGFRVERTSDKAYLKNYYNNY
ASYLTSKIFLNKIHEADYFSAEGLSFQGLGSNDRKYTDPLVLPKINTKNVINLNDNDNSHIVVENNTLMYKERIGKQVAR
TSLQLSFMHNVLTASGQIFDFAAKDRGDFYLVRRAYVDKPRESKAFQRNIPELQTLWRYPLVGNITKTINLLIEPVVSFT
MGRKLSKKDKKFVIIDPAKYELSEKNIFLSNRYSGIDCYDFGNRLIYGLNTSIAKEENYLKLFLGHSRNTRYSINLPADD
KENVGQILGNLSNNLEVFYSFRKDRYFRPIRDEVGGNFNCDKINFLGSFIQLTNLNKYYSVESIKVSNNRVRQFYGDINY
QLAENWTIGFGARIDLSRRSPNLLTRTIRMTYAKDCVRITAKIYSDYIADGNRGIKKTTSSPTISIGLKVLNM

Sequences:

>Translated_713_residues
MLLRIICLLIIIIFSLVSFAALSKNGQNIKISNIIADFVEYNENQDLIYAKGNIRIITDEYLLTVDNLLYDIENDILRAE
GNIRIKDKQNRIIEGDKAVLKDEFKKGIISEFILLSGDNNLLIAKLAERIDENNLKLHHAEFTPCDVACNRNPIWQIAAK
DTYIHSAEHRVVYKNVFFEVYGVPICYLPYFFHPTHTAPATSGLLVPDVKNKGFGIPLYLRAKPNIDFTLTPRIFSKYQT
YELEARYRLNDTDNMNFQVSYGKLPYFLEKDGSVVKNKKVSSYHYIASGNFISTDKVYDYGFRVERTSDKAYLKNYYNNY
ASYLTSKIFLNKIHEADYFSAEGLSFQGLGSNDRKYTDPLVLPKINTKNVINLNDNDNSHIVVENNTLMYKERIGKQVAR
TSLQLSFMHNVLTASGQIFDFAAKDRGDFYLVRRAYVDKPRESKAFQRNIPELQTLWRYPLVGNITKTINLLIEPVVSFT
MGRKLSKKDKKFVIIDPAKYELSEKNIFLSNRYSGIDCYDFGNRLIYGLNTSIAKEENYLKLFLGHSRNTRYSINLPADD
KENVGQILGNLSNNLEVFYSFRKDRYFRPIRDEVGGNFNCDKINFLGSFIQLTNLNKYYSVESIKVSNNRVRQFYGDINY
QLAENWTIGFGARIDLSRRSPNLLTRTIRMTYAKDCVRITAKIYSDYIADGNRGIKKTTSSPTISIGLKVLNM
>Mature_713_residues
MLLRIICLLIIIIFSLVSFAALSKNGQNIKISNIIADFVEYNENQDLIYAKGNIRIITDEYLLTVDNLLYDIENDILRAE
GNIRIKDKQNRIIEGDKAVLKDEFKKGIISEFILLSGDNNLLIAKLAERIDENNLKLHHAEFTPCDVACNRNPIWQIAAK
DTYIHSAEHRVVYKNVFFEVYGVPICYLPYFFHPTHTAPATSGLLVPDVKNKGFGIPLYLRAKPNIDFTLTPRIFSKYQT
YELEARYRLNDTDNMNFQVSYGKLPYFLEKDGSVVKNKKVSSYHYIASGNFISTDKVYDYGFRVERTSDKAYLKNYYNNY
ASYLTSKIFLNKIHEADYFSAEGLSFQGLGSNDRKYTDPLVLPKINTKNVINLNDNDNSHIVVENNTLMYKERIGKQVAR
TSLQLSFMHNVLTASGQIFDFAAKDRGDFYLVRRAYVDKPRESKAFQRNIPELQTLWRYPLVGNITKTINLLIEPVVSFT
MGRKLSKKDKKFVIIDPAKYELSEKNIFLSNRYSGIDCYDFGNRLIYGLNTSIAKEENYLKLFLGHSRNTRYSINLPADD
KENVGQILGNLSNNLEVFYSFRKDRYFRPIRDEVGGNFNCDKINFLGSFIQLTNLNKYYSVESIKVSNNRVRQFYGDINY
QLAENWTIGFGARIDLSRRSPNLLTRTIRMTYAKDCVRITAKIYSDYIADGNRGIKKTTSSPTISIGLKVLNM

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

None

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 82199; Mature: 82199

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLRIICLLIIIIFSLVSFAALSKNGQNIKISNIIADFVEYNENQDLIYAKGNIRIITDE
CHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCEEEECCCEEEEECH
YLLTVDNLLYDIENDILRAEGNIRIKDKQNRIIEGDKAVLKDEFKKGIISEFILLSGDNN
HHHHHHHHHHHHCCCEEECCCCEEEEECCCCEECCCHHHHHHHHHHHHHHEEEEEECCCC
LLIAKLAERIDENNLKLHHAEFTPCDVACNRNPIWQIAAKDTYIHSAEHRVVYKNVFFEV
EEHHHHHHHCCCCCEEEEECCCCCCEEECCCCCEEEEEECCEEEECCCCEEEEEEEEEHH
YGVPICYLPYFFHPTHTAPATSGLLVPDVKNKGFGIPLYLRAKPNIDFTLTPRIFSKYQT
HCCCEEEECEEECCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCEEECHHHHCCCEE
YELEARYRLNDTDNMNFQVSYGKLPYFLEKDGSVVKNKKVSSYHYIASGNFISTDKVYDY
EEEEEEEEECCCCCCEEEEEECCCCEEECCCCCEEECCCCCCEEEEEECCEEECCCEEEC
GFRVERTSDKAYLKNYYNNYASYLTSKIFLNKIHEADYFSAEGLSFQGLGSNDRKYTDPL
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCE
VLPKINTKNVINLNDNDNSHIVVENNTLMYKERIGKQVARTSLQLSFMHNVLTASGQIFD
EECCCCCCCEEEECCCCCCEEEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
FAAKDRGDFYLVRRAYVDKPRESKAFQRNIPELQTLWRYPLVGNITKTINLLIEPVVSFT
EECCCCCCEEEEEEHHHCCCCCCHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
MGRKLSKKDKKFVIIDPAKYELSEKNIFLSNRYSGIDCYDFGNRLIYGLNTSIAKEENYL
HCCCCCCCCCEEEEECCCCEEECCCEEEEECCCCCEEHEECCCEEEEECCCCCCCCCCEE
KLFLGHSRNTRYSINLPADDKENVGQILGNLSNNLEVFYSFRKDRYFRPIRDEVGGNFNC
EEEEECCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHCCCCCCC
DKINFLGSFIQLTNLNKYYSVESIKVSNNRVRQFYGDINYQLAENWTIGFGARIDLSRRS
CHHHHHHHHHHEECCCCEEEEEEEEECCHHHHHHHCCCCEEEECCEEEEEEEEEEECCCC
PNLLTRTIRMTYAKDCVRITAKIYSDYIADGNRGIKKTTSSPTISIGLKVLNM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MLLRIICLLIIIIFSLVSFAALSKNGQNIKISNIIADFVEYNENQDLIYAKGNIRIITDE
CHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCEEEECCCEEEEECH
YLLTVDNLLYDIENDILRAEGNIRIKDKQNRIIEGDKAVLKDEFKKGIISEFILLSGDNN
HHHHHHHHHHHHCCCEEECCCCEEEEECCCCEECCCHHHHHHHHHHHHHHEEEEEECCCC
LLIAKLAERIDENNLKLHHAEFTPCDVACNRNPIWQIAAKDTYIHSAEHRVVYKNVFFEV
EEHHHHHHHCCCCCEEEEECCCCCCEEECCCCCEEEEEECCEEEECCCCEEEEEEEEEHH
YGVPICYLPYFFHPTHTAPATSGLLVPDVKNKGFGIPLYLRAKPNIDFTLTPRIFSKYQT
HCCCEEEECEEECCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCEEECHHHHCCCEE
YELEARYRLNDTDNMNFQVSYGKLPYFLEKDGSVVKNKKVSSYHYIASGNFISTDKVYDY
EEEEEEEEECCCCCCEEEEEECCCCEEECCCCCEEECCCCCCEEEEEECCEEECCCEEEC
GFRVERTSDKAYLKNYYNNYASYLTSKIFLNKIHEADYFSAEGLSFQGLGSNDRKYTDPL
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCE
VLPKINTKNVINLNDNDNSHIVVENNTLMYKERIGKQVARTSLQLSFMHNVLTASGQIFD
EECCCCCCCEEEECCCCCCEEEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
FAAKDRGDFYLVRRAYVDKPRESKAFQRNIPELQTLWRYPLVGNITKTINLLIEPVVSFT
EECCCCCCEEEEEEHHHCCCCCCHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
MGRKLSKKDKKFVIIDPAKYELSEKNIFLSNRYSGIDCYDFGNRLIYGLNTSIAKEENYL
HCCCCCCCCCEEEEECCCCEEECCCEEEEECCCCCEEHEECCCEEEEECCCCCCCCCCEE
KLFLGHSRNTRYSINLPADDKENVGQILGNLSNNLEVFYSFRKDRYFRPIRDEVGGNFNC
EEEEECCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHCCCCCCC
DKINFLGSFIQLTNLNKYYSVESIKVSNNRVRQFYGDINYQLAENWTIGFGARIDLSRRS
CHHHHHHHHHHEECCCCEEEEEEEEECCHHHHHHHCCCCEEEECCEEEEEEEEEEECCCC
PNLLTRTIRMTYAKDCVRITAKIYSDYIADGNRGIKKTTSSPTISIGLKVLNM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]