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The map label for this gene is tuf [H]

Identifier: 15892931

GI number: 15892931

Start: 934451

End: 935635

Strand: Reverse

Name: tuf [H]

Synonym: RC1008

Alternate gene names: 15892931

Gene position: 935635-934451 (Counterclockwise)

Preceding gene: 15892933

Following gene: 15892930

Centisome position: 73.74

GC content: 38.99

Gene sequence:

>1185_bases
ATGGCAAAAGCAAAATTTGAACGAACTAAACCGCACGTTAATATAGGTACGATCGGTCACGTAGATCACGGTAAAACTTC
CTTAACGGCAGCAATAACTATAGTACTTGCTAAGACAGGTGGAGCACAAGCTACAGCGTATGATCAAATTGATGCTGCTC
CTGAAGAAAAAGAAAGAGGTATAACTATTTCTACTGCACACGTAGAATATGAGACTCAAAATAGACACTATGCACACGTA
GATTGTCCGGGACACGCTGACTATGTAAAGAACATGATAACCGGTGCCGCTCAGATGGACGGTGCAATATTAGTAGTTTC
TGCTGCTGATGGTCCTATGCCGCAAACTAGAGAACATATATTACTAGCAAAACAGGTAGGTGTGCCTGCTATGGTAGTGT
TCTTAAATAAAATAGATATGGTGGATGATCCTGATCTATTAGAACTAGTAGAGATGGAAGTAAGAGAATTATTATCAAAA
TATGGTTTCCCGGGTGATGAAATACCTATTATTAAAGGTTCTGCACTTCAAGCTTTAGAAGGAAAGCCTGAAGGTGAAAA
AGCTATTAATGAGCTAATGGATGCAGTAGATAGCTATATACCGCAGCCTGTAAGAGCTACGGATAAACCTTTCTTAATGC
CAATAGAAGATGTATTCTCTATTTCAGGAAGAGGCACTGTTGTAACTGGTAGAGTAGAGTCAGGAATAATTAAGGTAGGT
GAGGAAATTGAAATAGTTGGTCTAAAAGATACGCAAAAAACTACCTGTACCGGTGTAGAAATGTTCAGGAAATTACTTGA
TGAAGGACAAGCCGGAGATAATGTTGGTATATTACTACGCGGTACAAAAAGAGAAGAAGTTGAAAGAGGACAAGTTCTCG
CAAAACCTGGTAGCATAAAACCGCATGATAAGTTTGAAGCTGAAGTATATGTGCTTAGTAAAGAAGAAGGTGGACGTCAT
ACCCCATTTACTAATGACTATCGTCCGCAGTTCTATTTTAGAACAACAGATGTTACCGGTACAATAAAATTGTCTGCTGA
TAAGCAGATGGTTATGCCTGGAGATAATGCTACTTTTACAGTGGAATTAATTAAACCGATTGCGATGCAGGAAGGGTTGA
AATTCTCTATACGTGAAGGTGGTAGAACAGTGGGTGCCGGCGTAGTAACTAAAATAAATAATTAA

Upstream 100 bases:

>100_bases
ACCCGCTCCAGAGATAGTGGTAACAAATAAATTTAGCAAAAAAATGCACTTATAAAAAAATAACTTGTATTAAACCTAAA
AAATTGTAGAGAGTAATAAT

Downstream 100 bases:

>100_bases
TTGATTTTATAAATACCTATTATTTTTTAATAATAGGTATTTATATTTTAAGATGTAATAGACATTTTTCCGAACCGGCT
TATAGAGAAGAATTTGAAGG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKAKFERTKPHVNIGTIGHVDHGKTSLTAAITIVLAKTGGAQATAYDQIDAAPEEKERGITISTAHVEYETQNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLAKQVGVPAMVVFLNKIDMVDDPDLLELVEMEVRELLSK
YGFPGDEIPIIKGSALQALEGKPEGEKAINELMDAVDSYIPQPVRATDKPFLMPIEDVFSISGRGTVVTGRVESGIIKVG
EEIEIVGLKDTQKTTCTGVEMFRKLLDEGQAGDNVGILLRGTKREEVERGQVLAKPGSIKPHDKFEAEVYVLSKEEGGRH
TPFTNDYRPQFYFRTTDVTGTIKLSADKQMVMPGDNATFTVELIKPIAMQEGLKFSIREGGRTVGAGVVTKINN

Sequences:

>Translated_394_residues
MAKAKFERTKPHVNIGTIGHVDHGKTSLTAAITIVLAKTGGAQATAYDQIDAAPEEKERGITISTAHVEYETQNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLAKQVGVPAMVVFLNKIDMVDDPDLLELVEMEVRELLSK
YGFPGDEIPIIKGSALQALEGKPEGEKAINELMDAVDSYIPQPVRATDKPFLMPIEDVFSISGRGTVVTGRVESGIIKVG
EEIEIVGLKDTQKTTCTGVEMFRKLLDEGQAGDNVGILLRGTKREEVERGQVLAKPGSIKPHDKFEAEVYVLSKEEGGRH
TPFTNDYRPQFYFRTTDVTGTIKLSADKQMVMPGDNATFTVELIKPIAMQEGLKFSIREGGRTVGAGVVTKINN
>Mature_393_residues
AKAKFERTKPHVNIGTIGHVDHGKTSLTAAITIVLAKTGGAQATAYDQIDAAPEEKERGITISTAHVEYETQNRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLAKQVGVPAMVVFLNKIDMVDDPDLLELVEMEVRELLSKY
GFPGDEIPIIKGSALQALEGKPEGEKAINELMDAVDSYIPQPVRATDKPFLMPIEDVFSISGRGTVVTGRVESGIIKVGE
EIEIVGLKDTQKTTCTGVEMFRKLLDEGQAGDNVGILLRGTKREEVERGQVLAKPGSIKPHDKFEAEVYVLSKEEGGRHT
PFTNDYRPQFYFRTTDVTGTIKLSADKQMVMPGDNATFTVELIKPIAMQEGLKFSIREGGRTVGAGVVTKINN

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=391, Percent_Identity=58.0562659846547, Blast_Score=485, Evalue=1e-137,
Organism=Homo sapiens, GI223555963, Length=450, Percent_Identity=28.8888888888889, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI5729864, Length=450, Percent_Identity=28.8888888888889, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI4503475, Length=450, Percent_Identity=28.8888888888889, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI4503471, Length=450, Percent_Identity=28.8888888888889, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI54607086, Length=313, Percent_Identity=30.9904153354633, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI46094014, Length=330, Percent_Identity=30.3030303030303, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI194018522, Length=336, Percent_Identity=29.1666666666667, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI194097354, Length=336, Percent_Identity=29.1666666666667, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI194018520, Length=336, Percent_Identity=29.1666666666667, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI4503507, Length=359, Percent_Identity=28.6908077994429, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI82546879, Length=431, Percent_Identity=25.9860788863109, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI157426893, Length=253, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI94966754, Length=130, Percent_Identity=33.0769230769231, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=392, Percent_Identity=78.5714285714286, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI1789737, Length=392, Percent_Identity=78.5714285714286, Blast_Score=611, Evalue=1e-176,
Organism=Escherichia coli, GI2367247, Length=336, Percent_Identity=31.25, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI48994988, Length=277, Percent_Identity=31.4079422382672, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1789108, Length=316, Percent_Identity=26.5822784810127, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789559, Length=189, Percent_Identity=34.9206349206349, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788922, Length=132, Percent_Identity=31.8181818181818, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=398, Percent_Identity=52.5125628140703, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=45.7364341085271, Blast_Score=354, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI17552884, Length=451, Percent_Identity=30.5986696230599, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17569207, Length=451, Percent_Identity=30.5986696230599, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI32566303, Length=433, Percent_Identity=29.3302540415704, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI115532067, Length=440, Percent_Identity=27.5, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=440, Percent_Identity=27.5, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=357, Percent_Identity=28.8515406162465, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=271, Percent_Identity=29.8892988929889, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=148, Percent_Identity=37.1621621621622, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=279, Percent_Identity=26.1648745519713, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=232, Percent_Identity=26.7241379310345, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=66.1616161616162, Blast_Score=531, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6325337, Length=445, Percent_Identity=30.3370786516854, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=445, Percent_Identity=30.3370786516854, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=346, Percent_Identity=27.1676300578035, Blast_Score=106, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6320377, Length=355, Percent_Identity=27.0422535211268, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=275, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=252, Percent_Identity=24.2063492063492, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=178, Percent_Identity=30.3370786516854, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281363316, Length=390, Percent_Identity=58.974358974359, Blast_Score=487, Evalue=1e-138,
Organism=Drosophila melanogaster, GI17864358, Length=390, Percent_Identity=58.974358974359, Blast_Score=487, Evalue=1e-138,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=51.0362694300518, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI45553807, Length=455, Percent_Identity=30.5494505494506, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI45553816, Length=455, Percent_Identity=30.5494505494506, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24651721, Length=455, Percent_Identity=30.5494505494506, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI17864154, Length=455, Percent_Identity=30.5494505494506, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24652838, Length=450, Percent_Identity=30.4444444444444, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI17137572, Length=450, Percent_Identity=30.4444444444444, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=30.6930693069307, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI45550900, Length=437, Percent_Identity=27.0022883295195, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17137380, Length=339, Percent_Identity=31.2684365781711, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24647046, Length=370, Percent_Identity=27.027027027027, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24647048, Length=370, Percent_Identity=27.027027027027, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI78706572, Length=305, Percent_Identity=26.8852459016393, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582462, Length=146, Percent_Identity=31.5068493150685, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42869; Mature: 42737

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHVNIGTIGHVDHGKTSLTAAITIVLAKTGGAQATAYDQIDAAPEEKERG
CCCCCCCCCCCCEEECEEECCCCCCHHEEEEEEEEEEECCCCCEEEHHHCCCCCCHHHCC
ITISTAHVEYETQNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLAKQVGVPAMVVFLNKIDMVDDPDLLELVEMEVRELLSKYGFPGDEIPIIKGSALQALE
EEEHHCCCCEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHC
GKPEGEKAINELMDAVDSYIPQPVRATDKPFLMPIEDVFSISGRGTVVTGRVESGIIKVG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHCCCCCCEEEECCCCCCEEEEC
EEIEIVGLKDTQKTTCTGVEMFRKLLDEGQAGDNVGILLRGTKREEVERGQVLAKPGSIK
CEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHCCCEEECCCCCC
PHDKFEAEVYVLSKEEGGRHTPFTNDYRPQFYFRTTDVTGTIKLSADKQMVMPGDNATFT
CCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEEEECCCEEEECCCCCEEE
VELIKPIAMQEGLKFSIREGGRTVGAGVVTKINN
EEEHHHHHHHCCCEEEECCCCEEECCEEEEEECC
>Mature Secondary Structure 
AKAKFERTKPHVNIGTIGHVDHGKTSLTAAITIVLAKTGGAQATAYDQIDAAPEEKERG
CCCCCCCCCCCEEECEEECCCCCCHHEEEEEEEEEEECCCCCEEEHHHCCCCCCHHHCC
ITISTAHVEYETQNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLAKQVGVPAMVVFLNKIDMVDDPDLLELVEMEVRELLSKYGFPGDEIPIIKGSALQALE
EEEHHCCCCEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHC
GKPEGEKAINELMDAVDSYIPQPVRATDKPFLMPIEDVFSISGRGTVVTGRVESGIIKVG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHCCCCCCEEEECCCCCCEEEEC
EEIEIVGLKDTQKTTCTGVEMFRKLLDEGQAGDNVGILLRGTKREEVERGQVLAKPGSIK
CEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHCCCEEECCCCCC
PHDKFEAEVYVLSKEEGGRHTPFTNDYRPQFYFRTTDVTGTIKLSADKQMVMPGDNATFT
CCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEEEECCCEEEECCCCCEEE
VELIKPIAMQEGLKFSIREGGRTVGAGVVTKINN
EEEHHHHHHHCCCEEEECCCCEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA