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The map label for this gene is ogt [H]

Identifier: 15892790

GI number: 15892790

Start: 816906

End: 817082

Strand: Reverse

Name: ogt [H]

Synonym: RC0867

Alternate gene names: 15892790

Gene position: 817082-816906 (Counterclockwise)

Preceding gene: 15892791

Following gene: 15892789

Centisome position: 64.4

GC content: 34.46

Gene sequence:

>177_bases
TTGGGCAAACCTAATTCTTATAGAGCTGTTGCAAATGCTAATGGTATGAATCAGTTAGCAATCATTGTTCTTTGTCATCG
TATTATTAATAGCAATGGCAAACTTGGAGGTTATGGCAGGGGATTACATCGTAAAAAATGGCTTATTGAACATGAGAGAC
AATTTACAACAAATTAG

Upstream 100 bases:

>100_bases
CTATATAGCTTTTAGGAAGCCCATTTCAAAAAATGGTTTGGCAAGAATTAATGAATATACCTTACGGTGAAAACGAAGCT
ATTTAAATCAGGCTAAAGTT

Downstream 100 bases:

>100_bases
AAATATTTTACTTGAGAATGCTACTATTCCTGTAGAACGTAGAATGCTATTCTTGAAAACTAGAGAAGGAGAGTATGGAG
AGCATGACCGATTTATTGGT

Product: putative methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 58; Mature: 57

Protein sequence:

>58_residues
MGKPNSYRAVANANGMNQLAIIVLCHRIINSNGKLGGYGRGLHRKKWLIEHERQFTTN

Sequences:

>Translated_58_residues
MGKPNSYRAVANANGMNQLAIIVLCHRIINSNGKLGGYGRGLHRKKWLIEHERQFTTN
>Mature_57_residues
GKPNSYRAVANANGMNQLAIIVLCHRIINSNGKLGGYGRGLHRKKWLIEHERQFTTN

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1787596, Length=52, Percent_Identity=50, Blast_Score=64, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 6549; Mature: 6418

Theoretical pI: Translated: 11.22; Mature: 11.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKPNSYRAVANANGMNQLAIIVLCHRIINSNGKLGGYGRGLHRKKWLIEHERQFTTN
CCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
GKPNSYRAVANANGMNQLAIIVLCHRIINSNGKLGGYGRGLHRKKWLIEHERQFTTN
CCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]