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The map label for this gene is mraW [H]

Identifier: 15892780

GI number: 15892780

Start: 808574

End: 809497

Strand: Reverse

Name: mraW [H]

Synonym: RC0857

Alternate gene names: 15892780

Gene position: 809497-808574 (Counterclockwise)

Preceding gene: 15892781

Following gene: 15892779

Centisome position: 63.8

GC content: 37.12

Gene sequence:

>924_bases
ATGATACAATCTCACGTGTCTGTAATGCTAAATGAAATGTTAGAGGCCTTAAGCCCAAAAGCCGGTGAGTCTTATTTAGA
TTGTACGTTTGGAGCAGGAGGATATAGCAAAGCAATATTAGAAAGCTGTAACTGTTATGTCACAGCCTTGGATCGTGATC
CAAACGTGATTAAAAGAGCCGAAGAAATTCAGCAACATTACGGTGAGAGATTTGATTTTGTAGAAACAAATTTTGCGGAT
AGTTTTGCAAAGCTTAAGGAAAAAAAATTTGATGGGATAGTGCTGGATTTAGGCGTTTCATCAATGCAGTTAGATATAGC
AGATAGAGGATTTTCGTTTTTGCATGACGGACCACTCGATATGCGTATGAGCGGGCAAGGGCTTAGTGCCGAGGAGTTTG
TAAATGCGGCAGAGGAAAAAGAGCTAGCTGACGTAATTTACAAATACGGAGATGAGAGTTTTTCACGAAGAATTGCTAAG
AGAATTGTAGAATATAGAAAAACGGCGAGAATTGATAGTACAAGTAAGTTAGCCGAGATTGTTAGAAGTAGTATAGGATT
TAGAAAAGGCAAAATTGATCCTGCAACTAAAACCTTTCAAGCCATTAGGATTTATGTAAACGATGAGCTTGGGGAGTTAG
AGCAGTTTTTAGTGAATGTAAAAAATATATTAAAGAAAGACGGACGTTTAGTTGTGGTGTCTTTTCACTCTTTAGAAGAT
AGGATAGTTAAGAATTTCTTTAAAGAAAATTCGGAAAAGCCGGTAGTAAGATCAAAATATGCTAAAGACGACATGACGAT
TGATCCAAATAAATGGCTTAAGATTATTACTAACAAAGCTCTAGCACCGTCAGATAAAGAAGTAGGGCTAAATATTAGAG
CGAGATCGGCAAAACTTCGAGCAGCAAAAGCGATATATGAGTAA

Upstream 100 bases:

>100_bases
TGATTTTTGAGATTTGGCAGCCGCAGAATTTTGAGAAATATTTAAATGCCGCTCAAAAAATTGCTCATGAAAAGCGCTTA
ACTCTAAGGAATGCTCATTA

Downstream 100 bases:

>100_bases
TTAATTATGAGTATAAGAAAGCTTCACTATTTAACATTATTGATAACAATTATAGCAATATGTAGTTTGTTTAGCATAAA
GGAGAGAGTCTCAACTCTTG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MIQSHVSVMLNEMLEALSPKAGESYLDCTFGAGGYSKAILESCNCYVTALDRDPNVIKRAEEIQQHYGERFDFVETNFAD
SFAKLKEKKFDGIVLDLGVSSMQLDIADRGFSFLHDGPLDMRMSGQGLSAEEFVNAAEEKELADVIYKYGDESFSRRIAK
RIVEYRKTARIDSTSKLAEIVRSSIGFRKGKIDPATKTFQAIRIYVNDELGELEQFLVNVKNILKKDGRLVVVSFHSLED
RIVKNFFKENSEKPVVRSKYAKDDMTIDPNKWLKIITNKALAPSDKEVGLNIRARSAKLRAAKAIYE

Sequences:

>Translated_307_residues
MIQSHVSVMLNEMLEALSPKAGESYLDCTFGAGGYSKAILESCNCYVTALDRDPNVIKRAEEIQQHYGERFDFVETNFAD
SFAKLKEKKFDGIVLDLGVSSMQLDIADRGFSFLHDGPLDMRMSGQGLSAEEFVNAAEEKELADVIYKYGDESFSRRIAK
RIVEYRKTARIDSTSKLAEIVRSSIGFRKGKIDPATKTFQAIRIYVNDELGELEQFLVNVKNILKKDGRLVVVSFHSLED
RIVKNFFKENSEKPVVRSKYAKDDMTIDPNKWLKIITNKALAPSDKEVGLNIRARSAKLRAAKAIYE
>Mature_307_residues
MIQSHVSVMLNEMLEALSPKAGESYLDCTFGAGGYSKAILESCNCYVTALDRDPNVIKRAEEIQQHYGERFDFVETNFAD
SFAKLKEKKFDGIVLDLGVSSMQLDIADRGFSFLHDGPLDMRMSGQGLSAEEFVNAAEEKELADVIYKYGDESFSRRIAK
RIVEYRKTARIDSTSKLAEIVRSSIGFRKGKIDPATKTFQAIRIYVNDELGELEQFLVNVKNILKKDGRLVVVSFHSLED
RIVKNFFKENSEKPVVRSKYAKDDMTIDPNKWLKIITNKALAPSDKEVGLNIRARSAKLRAAKAIYE

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=338, Percent_Identity=40.2366863905325, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI165377202, Length=191, Percent_Identity=40.3141361256544, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI1786270, Length=306, Percent_Identity=42.483660130719, Blast_Score=224, Evalue=6e-60,
Organism=Drosophila melanogaster, GI62472493, Length=327, Percent_Identity=39.1437308868502, Blast_Score=194, Evalue=6e-50,
Organism=Drosophila melanogaster, GI28571637, Length=327, Percent_Identity=39.1437308868502, Blast_Score=194, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 34690; Mature: 34690

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQSHVSVMLNEMLEALSPKAGESYLDCTFGAGGYSKAILESCNCYVTALDRDPNVIKRA
CCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHCCCEEEEEECCCCHHHHHH
EEIQQHYGERFDFVETNFADSFAKLKEKKFDGIVLDLGVSSMQLDIADRGFSFLHDGPLD
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEHHHCCCHHHCCCCCC
MRMSGQGLSAEEFVNAAEEKELADVIYKYGDESFSRRIAKRIVEYRKTARIDSTSKLAEI
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VRSSIGFRKGKIDPATKTFQAIRIYVNDELGELEQFLVNVKNILKKDGRLVVVSFHSLED
HHHHCCCCCCCCCHHHHHEEHEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHH
RIVKNFFKENSEKPVVRSKYAKDDMTIDPNKWLKIITNKALAPSDKEVGLNIRARSAKLR
HHHHHHHHCCCCCCCHHHHHCCCCCEECHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHH
AAKAIYE
HHHHHCC
>Mature Secondary Structure
MIQSHVSVMLNEMLEALSPKAGESYLDCTFGAGGYSKAILESCNCYVTALDRDPNVIKRA
CCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHCCCEEEEEECCCCHHHHHH
EEIQQHYGERFDFVETNFADSFAKLKEKKFDGIVLDLGVSSMQLDIADRGFSFLHDGPLD
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEHHHCCCHHHCCCCCC
MRMSGQGLSAEEFVNAAEEKELADVIYKYGDESFSRRIAKRIVEYRKTARIDSTSKLAEI
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VRSSIGFRKGKIDPATKTFQAIRIYVNDELGELEQFLVNVKNILKKDGRLVVVSFHSLED
HHHHCCCCCCCCCHHHHHEEHEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHH
RIVKNFFKENSEKPVVRSKYAKDDMTIDPNKWLKIITNKALAPSDKEVGLNIRARSAKLR
HHHHHHHHCCCCCCCHHHHHCCCCCEECHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHH
AAKAIYE
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA