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The map label for this gene is tdcB [H]

Identifier: 15892551

GI number: 15892551

Start: 611878

End: 612909

Strand: Reverse

Name: tdcB [H]

Synonym: RC0628

Alternate gene names: 15892551

Gene position: 612909-611878 (Counterclockwise)

Preceding gene: 15892555

Following gene: 15892537

Centisome position: 48.31

GC content: 34.5

Gene sequence:

>1032_bases
ATGAATTTATTACTACAACCTCCTCAAAATGTAGCAGCCGCACATAATAGAATAAAACAATATCTACATTTAACTCCAAT
CGTTCACTCTGAAAGCTTAAATGAGATGCTCGGTCATGAGATTTTTTTTAAGGTTGAATCATTACAAAAAACCGGTGCAT
TTAAAGTTAGAGGCGTGTTAAATCATTTATTAGAATTAAAAGAGCAAGGAAAATTACCTGATAAAATAGTCGGTTATAGT
ACGGGTAACCATGGAATCGGTCTTGCTTATGCGAGTAAATTATTCGGTATTAAAACAAGGATTTATTTACCGTTAAATAC
TTCAAAAGTAAAACAGCAAGCAGCTCTTTATTACGGCGGTGAAGTTATATATACATATACTCGACAAGAAGCGGAAGAAA
AAGCAAAAGAAGATGAAAAGCAAGGATTTTACTATATGCATCCTTCCGATAGTGATTCTACAATAGCAGGAGCAGGCACG
TTATGCTATGAAGCATTACAGCAGCTAGGTTTTAGTCCTGATGCTATTTTTGCCTCTTGCGGCGGCGGCGGTTTAATTTC
CGGCGCATATCTTGCCAAGGAATTAATATCACCGACAAGCTTGTTAATAGGTTCAGAACCTTTAAATGCCAATGATGCTT
ATTTATCTGTTAAAGATAATAAAACATATAGATTTAACTATGCTCCGAATACTATTGCGGACGGTCTTAAAACCTTGAGT
ATATCAGCTCGTACATTTGAATATCTGAAAAAACTTGATGATTTTTATTTAGCAGAAGAATATGAAATATATTACTGGAC
GGCGTGGCTTACTCATTTACTTAAAGTAATATGCGAGCCGTCAAGTAGTATAAACATGGTTTCAGTGGTAAATTTTTTAA
AAACACAATCCAAACCTCAAAAATTACTAGTACTAATCTCAGGCGGTAATATCGATCCTATTCTTTATAATGAATTATGG
AAAGAGGAGTATTTAAGCATCTCACCTAAATCTTTATGTTCTGATGTCATTTCTGCGAAAGCAGCAATCTAG

Upstream 100 bases:

>100_bases
GGATTTTTTATGCATCAATTAAAACCTTTTAAAATTTCTAATTATTAACTATATAATATAGTATCAACCTGATTTCAAGC
TACCATTATTAATTATATTT

Downstream 100 bases:

>100_bases
TAACCTTTAATGTCATACTGCAACTTGATCGCGGTATCTTAGGACTCAGCTGGTGATACAAGACCCCGTGGTCACGCCAC
GGGGTGACAATAAAAAATCT

Product: threonine dehydratase

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MNLLLQPPQNVAAAHNRIKQYLHLTPIVHSESLNEMLGHEIFFKVESLQKTGAFKVRGVLNHLLELKEQGKLPDKIVGYS
TGNHGIGLAYASKLFGIKTRIYLPLNTSKVKQQAALYYGGEVIYTYTRQEAEEKAKEDEKQGFYYMHPSDSDSTIAGAGT
LCYEALQQLGFSPDAIFASCGGGGLISGAYLAKELISPTSLLIGSEPLNANDAYLSVKDNKTYRFNYAPNTIADGLKTLS
ISARTFEYLKKLDDFYLAEEYEIYYWTAWLTHLLKVICEPSSSINMVSVVNFLKTQSKPQKLLVLISGGNIDPILYNELW
KEEYLSISPKSLCSDVISAKAAI

Sequences:

>Translated_343_residues
MNLLLQPPQNVAAAHNRIKQYLHLTPIVHSESLNEMLGHEIFFKVESLQKTGAFKVRGVLNHLLELKEQGKLPDKIVGYS
TGNHGIGLAYASKLFGIKTRIYLPLNTSKVKQQAALYYGGEVIYTYTRQEAEEKAKEDEKQGFYYMHPSDSDSTIAGAGT
LCYEALQQLGFSPDAIFASCGGGGLISGAYLAKELISPTSLLIGSEPLNANDAYLSVKDNKTYRFNYAPNTIADGLKTLS
ISARTFEYLKKLDDFYLAEEYEIYYWTAWLTHLLKVICEPSSSINMVSVVNFLKTQSKPQKLLVLISGGNIDPILYNELW
KEEYLSISPKSLCSDVISAKAAI
>Mature_343_residues
MNLLLQPPQNVAAAHNRIKQYLHLTPIVHSESLNEMLGHEIFFKVESLQKTGAFKVRGVLNHLLELKEQGKLPDKIVGYS
TGNHGIGLAYASKLFGIKTRIYLPLNTSKVKQQAALYYGGEVIYTYTRQEAEEKAKEDEKQGFYYMHPSDSDSTIAGAGT
LCYEALQQLGFSPDAIFASCGGGGLISGAYLAKELISPTSLLIGSEPLNANDAYLSVKDNKTYRFNYAPNTIADGLKTLS
ISARTFEYLKKLDDFYLAEEYEIYYWTAWLTHLLKVICEPSSSINMVSVVNFLKTQSKPQKLLVLISGGNIDPILYNELW
KEEYLSISPKSLCSDVISAKAAI

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=311, Percent_Identity=34.4051446945338, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI1789505, Length=313, Percent_Identity=30.0319488817891, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1790207, Length=295, Percent_Identity=28.4745762711864, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI2367138, Length=199, Percent_Identity=25.6281407035176, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17508781, Length=320, Percent_Identity=28.75, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71991565, Length=309, Percent_Identity=27.5080906148867, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17537387, Length=315, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6322631, Length=313, Percent_Identity=32.9073482428115, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6320930, Length=276, Percent_Identity=28.6231884057971, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6319788, Length=263, Percent_Identity=25.0950570342205, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI21355833, Length=311, Percent_Identity=27.9742765273312, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 38134; Mature: 38134

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLLQPPQNVAAAHNRIKQYLHLTPIVHSESLNEMLGHEIFFKVESLQKTGAFKVRGVL
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHEEEHHHHHHCCCCHHHHHH
NHLLELKEQGKLPDKIVGYSTGNHGIGLAYASKLFGIKTRIYLPLNTSKVKQQAALYYGG
HHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHCEEEEEEEECCHHHHHHHHHHEECC
EVIYTYTRQEAEEKAKEDEKQGFYYMHPSDSDSTIAGAGTLCYEALQQLGFSPDAIFASC
EEEEEECHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEC
GGGGLISGAYLAKELISPTSLLIGSEPLNANDAYLSVKDNKTYRFNYAPNTIADGLKTLS
CCCCCHHHHHHHHHHCCCHHEEECCCCCCCCCCEEEEECCCEEEEECCCCHHHCCHHEEE
ISARTFEYLKKLDDFYLAEEYEIYYWTAWLTHLLKVICEPSSSINMVSVVNFLKTQSKPQ
EHHHHHHHHHHHHHHHEECCEEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCC
KLLVLISGGNIDPILYNELWKEEYLSISPKSLCSDVISAKAAI
EEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLLLQPPQNVAAAHNRIKQYLHLTPIVHSESLNEMLGHEIFFKVESLQKTGAFKVRGVL
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHEEEHHHHHHCCCCHHHHHH
NHLLELKEQGKLPDKIVGYSTGNHGIGLAYASKLFGIKTRIYLPLNTSKVKQQAALYYGG
HHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHCEEEEEEEECCHHHHHHHHHHEECC
EVIYTYTRQEAEEKAKEDEKQGFYYMHPSDSDSTIAGAGTLCYEALQQLGFSPDAIFASC
EEEEEECHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEC
GGGGLISGAYLAKELISPTSLLIGSEPLNANDAYLSVKDNKTYRFNYAPNTIADGLKTLS
CCCCCHHHHHHHHHHCCCHHEEECCCCCCCCCCEEEEECCCEEEEECCCCHHHCCHHEEE
ISARTFEYLKKLDDFYLAEEYEIYYWTAWLTHLLKVICEPSSSINMVSVVNFLKTQSKPQ
EHHHHHHHHHHHHHHHEECCEEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCC
KLLVLISGGNIDPILYNELWKEEYLSISPKSLCSDVISAKAAI
EEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]