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The map label for this gene is fabI [H]

Identifier: 15892417

GI number: 15892417

Start: 487888

End: 488670

Strand: Reverse

Name: fabI [H]

Synonym: RC0494

Alternate gene names: 15892417

Gene position: 488670-487888 (Counterclockwise)

Preceding gene: 15892424

Following gene: 15892410

Centisome position: 38.52

GC content: 35.12

Gene sequence:

>783_bases
ATGACTACAGGATTACTACAAGGAAAAAAAGGCTTAATTACAGGTATTGCCAATAATATGTCAATATCGTGGGCGATTGC
ACAGCTTGCTAAAAAACACGGAGCAGAACTATGGTTTACTTATCAATCGGGAGTACTAGAAAAACGAGTTAAACCACTCG
CTAAGGAAATAGGTTGTAATTTTGTTAGCGAACTTGATGTTACGAAGCCAAAATCAATTAGTAATTTATTTGATGATATA
AAAGAGAAATGGGGTAGCTTCGATTTCTTACTTCACGGTATGGCTTTTGCCGATAAAAATGAATTAAAAGGACGTTATGT
TGATACTAGCTTAGAAAATTTTCATAATTCTTTACATATATCATGTTACTCTCTTTTAGAACTCTCAAGGTCCGCAGAAG
CCTTAATGCATAACGGCGGAAGCATTGTGACATTAACTTACTACGGTGCTGAAAAAGTTATACCTAATTATAACGTTATG
GGAGTAGCCAAAGCTGCACTTGAGGCTAGTATAAAATATCTAGCAAACGATATGGGAGAAAATAATATTAGGGTTAATGC
TATTTCGGCAGGACCTATCAAAACTTTAGCATCTAGTGCAATAGGTGATTTTAGTACTATGCTTAAATCTCACGCTGCAA
CCGCACCGTTAAAACGTAATACTACCCAAGAAGATGTAGGAGGAGCAGCAGTATATTTATTTAGTGAATTATCTAAAGGT
GTTACCGGTGAAATCCATTATGTAGATTGCGGTTATAATATTATGGGAAGTACTAAATTATAA

Upstream 100 bases:

>100_bases
AACATATGATTTTAGGTTTATACATTTTTATCGATTATTTGTTTACAAAATAATACATCATGCTATCATTTCAGCAAGTT
TTAATATAATGAGTAAATAA

Downstream 100 bases:

>100_bases
ATTATTGTAGGGATTAATGTCATTCCCGCAAAAGGCTTGCTTGTGTAGACCAATAAAATCCGCTGTGTCACCCCGTGGCT
TGACCACAGGGTCCATAAAA

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTTGLLQGKKGLITGIANNMSISWAIAQLAKKHGAELWFTYQSGVLEKRVKPLAKEIGCNFVSELDVTKPKSISNLFDDI
KEKWGSFDFLLHGMAFADKNELKGRYVDTSLENFHNSLHISCYSLLELSRSAEALMHNGGSIVTLTYYGAEKVIPNYNVM
GVAKAALEASIKYLANDMGENNIRVNAISAGPIKTLASSAIGDFSTMLKSHAATAPLKRNTTQEDVGGAAVYLFSELSKG
VTGEIHYVDCGYNIMGSTKL

Sequences:

>Translated_260_residues
MTTGLLQGKKGLITGIANNMSISWAIAQLAKKHGAELWFTYQSGVLEKRVKPLAKEIGCNFVSELDVTKPKSISNLFDDI
KEKWGSFDFLLHGMAFADKNELKGRYVDTSLENFHNSLHISCYSLLELSRSAEALMHNGGSIVTLTYYGAEKVIPNYNVM
GVAKAALEASIKYLANDMGENNIRVNAISAGPIKTLASSAIGDFSTMLKSHAATAPLKRNTTQEDVGGAAVYLFSELSKG
VTGEIHYVDCGYNIMGSTKL
>Mature_259_residues
TTGLLQGKKGLITGIANNMSISWAIAQLAKKHGAELWFTYQSGVLEKRVKPLAKEIGCNFVSELDVTKPKSISNLFDDIK
EKWGSFDFLLHGMAFADKNELKGRYVDTSLENFHNSLHISCYSLLELSRSAEALMHNGGSIVTLTYYGAEKVIPNYNVMG
VAKAALEASIKYLANDMGENNIRVNAISAGPIKTLASSAIGDFSTMLKSHAATAPLKRNTTQEDVGGAAVYLFSELSKGV
TGEIHYVDCGYNIMGSTKL

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI10190704, Length=266, Percent_Identity=25.187969924812, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787545, Length=258, Percent_Identity=44.1860465116279, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1789378, Length=257, Percent_Identity=27.2373540856031, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87082100, Length=265, Percent_Identity=28.3018867924528, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI2367365, Length=253, Percent_Identity=27.6679841897233, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787905, Length=255, Percent_Identity=27.0588235294118, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71994604, Length=200, Percent_Identity=25.5, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28212; Mature: 28080

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGLLQGKKGLITGIANNMSISWAIAQLAKKHGAELWFTYQSGVLEKRVKPLAKEIGCN
CCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHCCC
FVSELDVTKPKSISNLFDDIKEKWGSFDFLLHGMAFADKNELKGRYVDTSLENFHNSLHI
HHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCEEECHHHHHHCCHHHH
SCYSLLELSRSAEALMHNGGSIVTLTYYGAEKVIPNYNVMGVAKAALEASIKYLANDMGE
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
NNIRVNAISAGPIKTLASSAIGDFSTMLKSHAATAPLKRNTTQEDVGGAAVYLFSELSKG
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHCCC
VTGEIHYVDCGYNIMGSTKL
CCCEEEEEECCCEECCCCCC
>Mature Secondary Structure 
TTGLLQGKKGLITGIANNMSISWAIAQLAKKHGAELWFTYQSGVLEKRVKPLAKEIGCN
CCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHCCC
FVSELDVTKPKSISNLFDDIKEKWGSFDFLLHGMAFADKNELKGRYVDTSLENFHNSLHI
HHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCEEECHHHHHHCCHHHH
SCYSLLELSRSAEALMHNGGSIVTLTYYGAEKVIPNYNVMGVAKAALEASIKYLANDMGE
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
NNIRVNAISAGPIKTLASSAIGDFSTMLKSHAATAPLKRNTTQEDVGGAAVYLFSELSKG
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHCCC
VTGEIHYVDCGYNIMGSTKL
CCCEEEEEECCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]