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The map label for this gene is eptB [H]

Identifier: 15892377

GI number: 15892377

Start: 448458

End: 450026

Strand: Reverse

Name: eptB [H]

Synonym: RC0454

Alternate gene names: 15892377

Gene position: 450026-448458 (Counterclockwise)

Preceding gene: 15892385

Following gene: 15892376

Centisome position: 35.47

GC content: 29.38

Gene sequence:

>1569_bases
ATGCTAAAAACTATTCAGAAACATTTAGATACGAAATTAATTAAATTATCGGCGATTTTAGCCTTTATTTATTGTTTATT
ATTTAATACCGCAATATTGATTTATAAATTTGATTATTATAAAGCAACAATCTTTAGAGGGATATTAGAGTTATCAAAAG
ATTTTTGCTATATTTATATATTTTCTTTTATAGCATTTTTCGGTCTTAGCGTACATAGATTAGTGTTAAAAATCGGCGTG
GGGTTTTTATTTATAACATCCGCTATTGCTAGTTATTACATTTATTTCTTCAAAATCAATCCTACTAAACAAGTAATAGG
TAGTTTTTTCTCAACGGATTTAAACGAAGTTTATGAATTAACCAGCATTAAGTTAATAATATGGATAATTTTTTGCCTTT
TTACCTGTTTTTATACTCTCAAATCTTTTGCCGCCGAAAATTCTAAATCATTCGTAACAAAACTATTATCTACCGCTTGC
TTACTTATTTTTGTATATAACATTATTACGCCTTCATTTAAGATACTAAAAAATTATTTTCCCATTCAGTATTTACATAA
TAGCTACCTTAATTTTGCCGGAAATTTCGGTGAAAAAAATTATGCATGTATAGATATTAGCAAACAATATAACTTTATAG
ATAAGTCAGATAAAGATATTATCGGTGTTTTAGTTATAGGAGAATCAGCAAGATTTGATCATTTCGGTATTAACGGTTAT
GAAAGAGATACGACTCCTTACTTAAAGACTACTCAAAACCTAATTTCTTTCAAAGCTAAATCTTCTTCTAATCTTACCTA
TCTTTCCGTACCGTCGTTACTTTCACGTTATCCTGCAAGTCAAATTGAAAATAGTAGACAAGAGAACAGCTTTTTATCAA
TTTTAACAAATCTTGGCTTTAATACTACCTGGATCGGTACTCAAACTTTAATGAGGAGTTTTGCAAATTTTGACCTCAGT
AATATATATAATGACGTTAATTTTACTATAGTACCGGGCGGCTCTGCCCTATTTTCTTTAAATGATCATGATGAAAAAAT
ATTGCCTTTTATAAAAGAAATACTAACAAACTCAGAGAAACAATTTTTAGTAGTTCACACTTCCGGCAGCCATTGGAACT
ATAATGCGAGATATCCAAAAGAGTTTGAGTATTTTACCCCTACCTGTCCTATTAAGGTTAAGGGAGATGCAAGTGATTGC
GATAAGCTTGCTTTAGTTAACAGTTATGATAACTCTATTTTATATACCGATTTTTTTTTATATAATTTAATAGATTTGCT
TAAAGATAAAAATGCATTTTTATTATATGTATCTGATCACGGCGAGTCCCTCGGAGAAAATGGTTATTACGGTCACGGCG
GTCCACTACTTGCAGAACAAATAACCGTACCTCTTATAGTTTGGGTATCAGATGATTTTCAAGCCAAATATCCTAAATCA
GTATCTTCAATTAAAAATTATGCTAATACCGAAATTAGCCATGATTATGTATTTCATTCAATCCTTAACTGTTTAAATAT
AGAGTCCGACATAATAGATAAAGACTTAAGTATATGTAAATCTAGTTAA

Upstream 100 bases:

>100_bases
ATAGCGATAAGCGGAACATTTGGTATTAAAAGCCGATAAAATTCTTGAAGATTTTTTATGATCATGATAGTTATAGAAAT
TACTTTATTAATTATTATTT

Downstream 100 bases:

>100_bases
AAATTATTGAATTATTAAAAAATTTATATATAAATTAAGAACTCAATAATTATATGATTTATTATTATGTCTGATATACC
TAAACTAGACGACTATTATT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MLKTIQKHLDTKLIKLSAILAFIYCLLFNTAILIYKFDYYKATIFRGILELSKDFCYIYIFSFIAFFGLSVHRLVLKIGV
GFLFITSAIASYYIYFFKINPTKQVIGSFFSTDLNEVYELTSIKLIIWIIFCLFTCFYTLKSFAAENSKSFVTKLLSTAC
LLIFVYNIITPSFKILKNYFPIQYLHNSYLNFAGNFGEKNYACIDISKQYNFIDKSDKDIIGVLVIGESARFDHFGINGY
ERDTTPYLKTTQNLISFKAKSSSNLTYLSVPSLLSRYPASQIENSRQENSFLSILTNLGFNTTWIGTQTLMRSFANFDLS
NIYNDVNFTIVPGGSALFSLNDHDEKILPFIKEILTNSEKQFLVVHTSGSHWNYNARYPKEFEYFTPTCPIKVKGDASDC
DKLALVNSYDNSILYTDFFLYNLIDLLKDKNAFLLYVSDHGESLGENGYYGHGGPLLAEQITVPLIVWVSDDFQAKYPKS
VSSIKNYANTEISHDYVFHSILNCLNIESDIIDKDLSICKSS

Sequences:

>Translated_522_residues
MLKTIQKHLDTKLIKLSAILAFIYCLLFNTAILIYKFDYYKATIFRGILELSKDFCYIYIFSFIAFFGLSVHRLVLKIGV
GFLFITSAIASYYIYFFKINPTKQVIGSFFSTDLNEVYELTSIKLIIWIIFCLFTCFYTLKSFAAENSKSFVTKLLSTAC
LLIFVYNIITPSFKILKNYFPIQYLHNSYLNFAGNFGEKNYACIDISKQYNFIDKSDKDIIGVLVIGESARFDHFGINGY
ERDTTPYLKTTQNLISFKAKSSSNLTYLSVPSLLSRYPASQIENSRQENSFLSILTNLGFNTTWIGTQTLMRSFANFDLS
NIYNDVNFTIVPGGSALFSLNDHDEKILPFIKEILTNSEKQFLVVHTSGSHWNYNARYPKEFEYFTPTCPIKVKGDASDC
DKLALVNSYDNSILYTDFFLYNLIDLLKDKNAFLLYVSDHGESLGENGYYGHGGPLLAEQITVPLIVWVSDDFQAKYPKS
VSSIKNYANTEISHDYVFHSILNCLNIESDIIDKDLSICKSS
>Mature_522_residues
MLKTIQKHLDTKLIKLSAILAFIYCLLFNTAILIYKFDYYKATIFRGILELSKDFCYIYIFSFIAFFGLSVHRLVLKIGV
GFLFITSAIASYYIYFFKINPTKQVIGSFFSTDLNEVYELTSIKLIIWIIFCLFTCFYTLKSFAAENSKSFVTKLLSTAC
LLIFVYNIITPSFKILKNYFPIQYLHNSYLNFAGNFGEKNYACIDISKQYNFIDKSDKDIIGVLVIGESARFDHFGINGY
ERDTTPYLKTTQNLISFKAKSSSNLTYLSVPSLLSRYPASQIENSRQENSFLSILTNLGFNTTWIGTQTLMRSFANFDLS
NIYNDVNFTIVPGGSALFSLNDHDEKILPFIKEILTNSEKQFLVVHTSGSHWNYNARYPKEFEYFTPTCPIKVKGDASDC
DKLALVNSYDNSILYTDFFLYNLIDLLKDKNAFLLYVSDHGESLGENGYYGHGGPLLAEQITVPLIVWVSDDFQAKYPKS
VSSIKNYANTEISHDYVFHSILNCLNIESDIIDKDLSICKSS

Specific function: Catalyzes the addition of a phosphoethanolamine (pEtN) moiety to the outer 3-deoxy-D-manno-octulosonic acid (KDO) residue of a KDO(2)-lipid A. Phosphatidylethanolamines with one unsaturated acyl group functions as pEtN donors and the reaction releases dia

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082286, Length=542, Percent_Identity=27.4907749077491, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI87082372, Length=325, Percent_Identity=30.4615384615385, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI87082223, Length=364, Percent_Identity=27.1978021978022, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1790392, Length=304, Percent_Identity=27.6315789473684, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787035, Length=276, Percent_Identity=26.8115942028986, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 59694; Mature: 59694

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTIQKHLDTKLIKLSAILAFIYCLLFNTAILIYKFDYYKATIFRGILELSKDFCYIYI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
FSFIAFFGLSVHRLVLKIGVGFLFITSAIASYYIYFFKINPTKQVIGSFFSTDLNEVYEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHCCHHHHHHH
TSIKLIIWIIFCLFTCFYTLKSFAAENSKSFVTKLLSTACLLIFVYNIITPSFKILKNYF
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
PIQYLHNSYLNFAGNFGEKNYACIDISKQYNFIDKSDKDIIGVLVIGESARFDHFGINGY
CHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCC
ERDTTPYLKTTQNLISFKAKSSSNLTYLSVPSLLSRYPASQIENSRQENSFLSILTNLGF
CCCCCCHHHHHHHHHHEECCCCCCEEEEEHHHHHHHCCHHHHHCCHHHHHHHHHHHHCCC
NTTWIGTQTLMRSFANFDLSNIYNDVNFTIVPGGSALFSLNDHDEKILPFIKEILTNSEK
CEEEECHHHHHHHHHCCCHHHHHCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHCCCCC
QFLVVHTSGSHWNYNARYPKEFEYFTPTCPIKVKGDASDCDKLALVNSYDNSILYTDFFL
EEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEECCCCCEEHHHHHH
YNLIDLLKDKNAFLLYVSDHGESLGENGYYGHGGPLLAEQITVPLIVWVSDDFQAKYPKS
HHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCHH
VSSIKNYANTEISHDYVFHSILNCLNIESDIIDKDLSICKSS
HHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKTIQKHLDTKLIKLSAILAFIYCLLFNTAILIYKFDYYKATIFRGILELSKDFCYIYI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
FSFIAFFGLSVHRLVLKIGVGFLFITSAIASYYIYFFKINPTKQVIGSFFSTDLNEVYEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHCCHHHHHHH
TSIKLIIWIIFCLFTCFYTLKSFAAENSKSFVTKLLSTACLLIFVYNIITPSFKILKNYF
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
PIQYLHNSYLNFAGNFGEKNYACIDISKQYNFIDKSDKDIIGVLVIGESARFDHFGINGY
CHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCC
ERDTTPYLKTTQNLISFKAKSSSNLTYLSVPSLLSRYPASQIENSRQENSFLSILTNLGF
CCCCCCHHHHHHHHHHEECCCCCCEEEEEHHHHHHHCCHHHHHCCHHHHHHHHHHHHCCC
NTTWIGTQTLMRSFANFDLSNIYNDVNFTIVPGGSALFSLNDHDEKILPFIKEILTNSEK
CEEEECHHHHHHHHHCCCHHHHHCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHCCCCC
QFLVVHTSGSHWNYNARYPKEFEYFTPTCPIKVKGDASDCDKLALVNSYDNSILYTDFFL
EEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEECCCCCEEHHHHHH
YNLIDLLKDKNAFLLYVSDHGESLGENGYYGHGGPLLAEQITVPLIVWVSDDFQAKYPKS
HHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCHH
VSSIKNYANTEISHDYVFHSILNCLNIESDIIDKDLSICKSS
HHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677609; 7721701 [H]