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The map label for this gene is nuoL2 [H]

Identifier: 15892304

GI number: 15892304

Start: 381979

End: 383442

Strand: Reverse

Name: nuoL2 [H]

Synonym: RC0381

Alternate gene names: 15892304

Gene position: 383442-381979 (Counterclockwise)

Preceding gene: 15892305

Following gene: 15892303

Centisome position: 30.22

GC content: 30.19

Gene sequence:

>1464_bases
ATGATTACACAATTTACTACTCCGAATCTTTTGATTCTATCAACTTTGCTAGTTGGTGCATTAAACTTAACTAGCCCGTA
TGCCACTCAAAAAGATAGCTTTATACGTAATTTTTTACTTATTACTATTGCTATTTTTTTCTTCGGTAATATTTTAATTA
TAGACTGGTTGTTTTTAAAAGGTATTAGAGCAGGATTTGAATTTAATATATTCGGTAATTACTCTATAGGATTCCATCTT
GAACCTTTAGGGCTTATTTTCTTAAGTTTAATAGGCTTTTTATGGATTTGTGCTTTACTTTACACTTCTAAATATCTTGC
TATTAATAATATAGACTATTCTTCTAGATTCTTATTTTTCTTTAACTTCACTATTCTAATCGGTGTTTTAATTGCTTTAT
CTAGCAACTTATTCACGATGTTTATTTGCTATGAGCTTTTAACTATTTCTACAGCTTTTTTAATAGGACATACCAAAAAT
AATATAGTACTTAGCGGGTTATATAAATATCTAAAAATTCTGATGATTTCTGCCACAATATTATTTTTACCGGCAGTGAT
AATTATTTATACTAAAACCGGTAACGGAGATTTTGCAAGTGGGAGTTTAGTAGAAAATTATTTTTCCAAAAACCAGTCAA
TTATTTTATTGTTAATGTTTATTTGCGGTATTGCTAAAACTGCCATTTTTCCGGTGCATTCATGGCTTCCTGCTGCAATG
GTTGCACATTACCCCATTAGTAGTTTGCTGCATGCCGTAATAGTGGTTAAAACCGGATTATTTTGTATTTACAAAATCCT
ATTATATATATTCGGTTTATCGTATTTACAAATGATATTTGCCGAGTTTAACTGGTTAATTTTTATACCGATAGTAAGCA
TATTTTATAGCTCAATCAAAGCCCTAGACACGGATAATATCAAAAAAATACTCGCCTACTCCACTATGAACCAATTAAGC
CTTGCATTGCTTAGTGCTTTCATGTTAACACCTAAGGCACTTGGAGCCGCAATACTCCATTTAGTTTCACATTCTTTTAC
AAAAATCTGCTTATTTTATAGTATGGGAAGTATTTACAGTTTAAAGAAAACCAATCAAGTACAGAATTTACATGGTACAT
CCAAAGAATTTCCTTTAATTTCTTTTATTATATCTATCTCTTCATTATCATTAATCGGTATACCTATCTTTAGCGGATTT
ATTAGCAAATTCTCAATATTACTTGCCGCAAGCGAACAGAATCAAATCATTGTTATGATTGTCGTAATAGCTAGTAGTAT
ATTCTCAAGCCTTTACCTGCTCAAAATATTAAGCTCTATTTATAAACCTTCTTTAGTAGAATCTAGCGTAACAAAACCAC
TCCCCTACTCTATGCAAATTAGTATAATAATGTGCTGCTGTGCCGTAACTCTTTTCTATTTTATTCAAATCTTAATCAGA
GAATTTTTAGCTTATATTACATAA

Upstream 100 bases:

>100_bases
TCGCTTTTTCTGGATTCCCCCACTTTTAGCTAGGCATGACATAGAAAGCCATACAGGTAATGCTCTAACCATACTATCAA
AAAAAGTATAACAGCTTATC

Downstream 100 bases:

>100_bases
ACATATTTACATAATATACACATAATCATATAATATGTATACCGTAGATACTTCAAAAGTTGGAAGTCAAATAAGCAGTG
CACCTACACAGCGTACAATA

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol

Alternate protein names: Mrp complex subunit A2; Putative NADH-ubiquinone oxidoreductase subunit mnhA2 [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MITQFTTPNLLILSTLLVGALNLTSPYATQKDSFIRNFLLITIAIFFFGNILIIDWLFLKGIRAGFEFNIFGNYSIGFHL
EPLGLIFLSLIGFLWICALLYTSKYLAINNIDYSSRFLFFFNFTILIGVLIALSSNLFTMFICYELLTISTAFLIGHTKN
NIVLSGLYKYLKILMISATILFLPAVIIIYTKTGNGDFASGSLVENYFSKNQSIILLLMFICGIAKTAIFPVHSWLPAAM
VAHYPISSLLHAVIVVKTGLFCIYKILLYIFGLSYLQMIFAEFNWLIFIPIVSIFYSSIKALDTDNIKKILAYSTMNQLS
LALLSAFMLTPKALGAAILHLVSHSFTKICLFYSMGSIYSLKKTNQVQNLHGTSKEFPLISFIISISSLSLIGIPIFSGF
ISKFSILLAASEQNQIIVMIVVIASSIFSSLYLLKILSSIYKPSLVESSVTKPLPYSMQISIIMCCCAVTLFYFIQILIR
EFLAYIT

Sequences:

>Translated_487_residues
MITQFTTPNLLILSTLLVGALNLTSPYATQKDSFIRNFLLITIAIFFFGNILIIDWLFLKGIRAGFEFNIFGNYSIGFHL
EPLGLIFLSLIGFLWICALLYTSKYLAINNIDYSSRFLFFFNFTILIGVLIALSSNLFTMFICYELLTISTAFLIGHTKN
NIVLSGLYKYLKILMISATILFLPAVIIIYTKTGNGDFASGSLVENYFSKNQSIILLLMFICGIAKTAIFPVHSWLPAAM
VAHYPISSLLHAVIVVKTGLFCIYKILLYIFGLSYLQMIFAEFNWLIFIPIVSIFYSSIKALDTDNIKKILAYSTMNQLS
LALLSAFMLTPKALGAAILHLVSHSFTKICLFYSMGSIYSLKKTNQVQNLHGTSKEFPLISFIISISSLSLIGIPIFSGF
ISKFSILLAASEQNQIIVMIVVIASSIFSSLYLLKILSSIYKPSLVESSVTKPLPYSMQISIIMCCCAVTLFYFIQILIR
EFLAYIT
>Mature_487_residues
MITQFTTPNLLILSTLLVGALNLTSPYATQKDSFIRNFLLITIAIFFFGNILIIDWLFLKGIRAGFEFNIFGNYSIGFHL
EPLGLIFLSLIGFLWICALLYTSKYLAINNIDYSSRFLFFFNFTILIGVLIALSSNLFTMFICYELLTISTAFLIGHTKN
NIVLSGLYKYLKILMISATILFLPAVIIIYTKTGNGDFASGSLVENYFSKNQSIILLLMFICGIAKTAIFPVHSWLPAAM
VAHYPISSLLHAVIVVKTGLFCIYKILLYIFGLSYLQMIFAEFNWLIFIPIVSIFYSSIKALDTDNIKKILAYSTMNQLS
LALLSAFMLTPKALGAAILHLVSHSFTKICLFYSMGSIYSLKKTNQVQNLHGTSKEFPLISFIISISSLSLIGIPIFSGF
ISKFSILLAASEQNQIIVMIVVIASSIFSSLYLLKILSSIYKPSLVESSVTKPLPYSMQISIIMCCCAVTLFYFIQILIR
EFLAYIT

Specific function: Ndh-1 Shuttles Electrons From NADH, Via Fmn And Iron- Sulfur (Fe-S) Centers, To Quinones In The Respiratory Chain. The Immediate Electron Acceptor For The Enzyme In This Species Is Believed To Be Ubiquinone. Couples The Redox Reaction To Proton Translocat

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=400, Percent_Identity=26.5, Blast_Score=96, Evalue=9e-20,
Organism=Escherichia coli, GI1788614, Length=379, Percent_Identity=26.6490765171504, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788831, Length=358, Percent_Identity=27.3743016759777, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788829, Length=396, Percent_Identity=25.7575757575758, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1788827, Length=318, Percent_Identity=26.4150943396226, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788613, Length=412, Percent_Identity=23.7864077669903, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI145693160, Length=357, Percent_Identity=24.0896358543417, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI2367154, Length=347, Percent_Identity=22.478386167147, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 54556; Mature: 54556

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITQFTTPNLLILSTLLVGALNLTSPYATQKDSFIRNFLLITIAIFFFGNILIIDWLFLK
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIRAGFEFNIFGNYSIGFHLEPLGLIFLSLIGFLWICALLYTSKYLAINNIDYSSRFLFF
HHHCCEEEEEEECCEECEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHH
FNFTILIGVLIALSSNLFTMFICYELLTISTAFLIGHTKNNIVLSGLYKYLKILMISATI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHH
LFLPAVIIIYTKTGNGDFASGSLVENYFSKNQSIILLLMFICGIAKTAIFPVHSWLPAAM
HHHHHHHEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAHYPISSLLHAVIVVKTGLFCIYKILLYIFGLSYLQMIFAEFNWLIFIPIVSIFYSSIK
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALDTDNIKKILAYSTMNQLSLALLSAFMLTPKALGAAILHLVSHSFTKICLFYSMGSIYS
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LKKTNQVQNLHGTSKEFPLISFIISISSLSLIGIPIFSGFISKFSILLAASEQNQIIVMI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEHH
VVIASSIFSSLYLLKILSSIYKPSLVESSVTKPLPYSMQISIIMCCCAVTLFYFIQILIR
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EFLAYIT
HHHHHCC
>Mature Secondary Structure
MITQFTTPNLLILSTLLVGALNLTSPYATQKDSFIRNFLLITIAIFFFGNILIIDWLFLK
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIRAGFEFNIFGNYSIGFHLEPLGLIFLSLIGFLWICALLYTSKYLAINNIDYSSRFLFF
HHHCCEEEEEEECCEECEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHH
FNFTILIGVLIALSSNLFTMFICYELLTISTAFLIGHTKNNIVLSGLYKYLKILMISATI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHH
LFLPAVIIIYTKTGNGDFASGSLVENYFSKNQSIILLLMFICGIAKTAIFPVHSWLPAAM
HHHHHHHEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAHYPISSLLHAVIVVKTGLFCIYKILLYIFGLSYLQMIFAEFNWLIFIPIVSIFYSSIK
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALDTDNIKKILAYSTMNQLSLALLSAFMLTPKALGAAILHLVSHSFTKICLFYSMGSIYS
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LKKTNQVQNLHGTSKEFPLISFIISISSLSLIGIPIFSGFISKFSILLAASEQNQIIVMI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEHH
VVIASSIFSSLYLLKILSSIYKPSLVESSVTKPLPYSMQISIIMCCCAVTLFYFIQILIR
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EFLAYIT
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA