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The map label for this gene is ptrB [C]

Identifier: 15892138

GI number: 15892138

Start: 225636

End: 225932

Strand: Reverse

Name: ptrB [C]

Synonym: RC0215

Alternate gene names: 15892138

Gene position: 225932-225636 (Counterclockwise)

Preceding gene: 15892139

Following gene: 15892134

Centisome position: 17.81

GC content: 40.07

Gene sequence:

>297_bases
ATGGAAAATGAAGTATGGGTTAAACATGGCAGGGTTAGTGTTCTTGCTAATATTAGAGGTGGCGGAGAGTTTGGTCCTGA
GTGGCATGAGGCGACTCAAGGAATAAAACGTCAAACCGGCTTGAATGATTTTATTGCAGTAGCAGAGGATTTAATAAAGC
AAAATATTACTAGTCCTGAGTATTTAGGAATTAAAGGAGGAAGTCACGGCGGATTGCTAGTAAGTGTTGCAATGACACAG
TGTCCTGATTTATTTGGAGCTATAGCATGTGAAGTGCCGATACTTGATACGATATAA

Upstream 100 bases:

>100_bases
ATTTTATTGTCTATAAAAAAGGTACAAAGATTGTAAGAATCCAACATTACTTGAAGCATACAGCGGTTTTCAAGTAATCA
ACTCTCCATATTTCTCTCGC

Downstream 100 bases:

>100_bases
AGAATTCGGAGCAGGACATTCATGGGGTACAGAATATGGTGATCCAAAGTAGAAGATTTAGTACATATCAAAAAATATGC
ACCGCTAGAGAATTTATCTT

Product: putative prolyl endopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 98; Mature: 98

Protein sequence:

>98_residues
MENEVWVKHGRVSVLANIRGGGEFGPEWHEATQGIKRQTGLNDFIAVAEDLIKQNITSPEYLGIKGGSHGGLLVSVAMTQ
CPDLFGAIACEVPILDTI

Sequences:

>Translated_98_residues
MENEVWVKHGRVSVLANIRGGGEFGPEWHEATQGIKRQTGLNDFIAVAEDLIKQNITSPEYLGIKGGSHGGLLVSVAMTQ
CPDLFGAIACEVPILDTI
>Mature_98_residues
MENEVWVKHGRVSVLANIRGGGEFGPEWHEATQGIKRQTGLNDFIAVAEDLIKQNITSPEYLGIKGGSHGGLLVSVAMTQ
CPDLFGAIACEVPILDTI

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=95, Percent_Identity=45.2631578947368, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788150, Length=89, Percent_Identity=35.9550561797753, Blast_Score=74, Evalue=2e-15,
Organism=Drosophila melanogaster, GI221510989, Length=87, Percent_Identity=45.9770114942529, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583414, Length=82, Percent_Identity=45.1219512195122, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 10517; Mature: 10517

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENEVWVKHGRVSVLANIRGGGEFGPEWHEATQGIKRQTGLNDFIAVAEDLIKQNITSPE
CCCCEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
YLGIKGGSHGGLLVSVAMTQCPDLFGAIACEVPILDTI
EEEECCCCCCCEEEEHHHHHCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MENEVWVKHGRVSVLANIRGGGEFGPEWHEATQGIKRQTGLNDFIAVAEDLIKQNITSPE
CCCCEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
YLGIKGGSHGGLLVSVAMTQCPDLFGAIACEVPILDTI
EEEECCCCCCCEEEEHHHHHCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.23 {tosyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 80 {acetyl-tyrosine} 0.47 {tosyl-Lys-methyl} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]