Click here to switch to the map view.

The map label for this gene is folA [H]

Identifier: 15891957

GI number: 15891957

Start: 34249

End: 34743

Strand: Reverse

Name: folA [H]

Synonym: RC0034

Alternate gene names: 15891957

Gene position: 34743-34249 (Counterclockwise)

Preceding gene: 15891958

Following gene: 15891956

Centisome position: 2.74

GC content: 26.46

Gene sequence:

>495_bases
ATGAAAAATAGAAAAATCATCGGTATAATGGCATGTGACCCACAAGGGGTGATAGGTAATAAAGGTCAGATGCCTTGGTC
TTATACTAAGGAGTTTGAATATTTTTATCAGACGGTAAAAAATAATATTATAGTCATGGGACGAAAAACCTTTGACTCTA
TACCTACAAAGATTTTAAAAAATTGTATTTGTATTGTTTTTTCAAGAAATACACCATTACAATCTTATAATAATATATTT
TTTATTAAATCCTTAGATGATTTTTGGCAGGTAATAAAACCTTTTACTGATAAAAAAATTTTTATGGTTGGAGGGACAAA
AATAGCAACTCTTTTCCTAGAACAAAATCTTATTGATGAATTTTTACTTACAAAAATAAATAAAAATTATGATGGAGATA
CTTTTTTGCCTCTTAATCTTTTAGCAGAATGGCATTCTGTAATAATAGATAAAACAAACAATTATCAGATTTATAAATTT
ACTAAGAGGAGGTAA

Upstream 100 bases:

>100_bases
CAAAATATTTAATGGAAAACTTAGTTGATTATTTACAAGTGCATAATGTTATAGAGCATCAAAAATTTTTTGTAGCAGAC
CATATGATTAACTATATCTT

Downstream 100 bases:

>100_bases
GATGAAATTTTTAGAACAGCTTAATATAAAAATCTTGAGCAATATAACCTCTTAAATCATCCATTTTTCAATTATGGCAT
ATGGGAAAACTTACTCATTC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MKNRKIIGIMACDPQGVIGNKGQMPWSYTKEFEYFYQTVKNNIIVMGRKTFDSIPTKILKNCICIVFSRNTPLQSYNNIF
FIKSLDDFWQVIKPFTDKKIFMVGGTKIATLFLEQNLIDEFLLTKINKNYDGDTFLPLNLLAEWHSVIIDKTNNYQIYKF
TKRR

Sequences:

>Translated_164_residues
MKNRKIIGIMACDPQGVIGNKGQMPWSYTKEFEYFYQTVKNNIIVMGRKTFDSIPTKILKNCICIVFSRNTPLQSYNNIF
FIKSLDDFWQVIKPFTDKKIFMVGGTKIATLFLEQNLIDEFLLTKINKNYDGDTFLPLNLLAEWHSVIIDKTNNYQIYKF
TKRR
>Mature_164_residues
MKNRKIIGIMACDPQGVIGNKGQMPWSYTKEFEYFYQTVKNNIIVMGRKTFDSIPTKILKNCICIVFSRNTPLQSYNNIF
FIKSLDDFWQVIKPFTDKKIFMVGGTKIATLFLEQNLIDEFLLTKINKNYDGDTFLPLNLLAEWHSVIIDKTNNYQIYKF
TKRR

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17505899, Length=146, Percent_Identity=36.3013698630137, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19302; Mature: 19302

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRKIIGIMACDPQGVIGNKGQMPWSYTKEFEYFYQTVKNNIIVMGRKTFDSIPTKILK
CCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHH
NCICIVFSRNTPLQSYNNIFFIKSLDDFWQVIKPFTDKKIFMVGGTKIATLFLEQNLIDE
HEEEEEEECCCCCCCCCCEEEEECHHHHHHHHCCCCCCEEEEECCCEEEEEHHHHHHHHH
FLLTKINKNYDGDTFLPLNLLAEWHSVIIDKTNNYQIYKFTKRR
HHHHHHCCCCCCCEEEHHHHHHHHHHEEEECCCCEEEEEEECCC
>Mature Secondary Structure
MKNRKIIGIMACDPQGVIGNKGQMPWSYTKEFEYFYQTVKNNIIVMGRKTFDSIPTKILK
CCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHH
NCICIVFSRNTPLQSYNNIFFIKSLDDFWQVIKPFTDKKIFMVGGTKIATLFLEQNLIDE
HEEEEEEECCCCCCCCCCEEEEECHHHHHHHHCCCCCCEEEEECCCEEEEEHHHHHHHHH
FLLTKINKNYDGDTFLPLNLLAEWHSVIIDKTNNYQIYKFTKRR
HHHHHHCCCCCCCEEEHHHHHHHHHHEEEECCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7574597; 11337471 [H]