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The map label for this gene is phoB [H]

Identifier: 158425656

GI number: 158425656

Start: 4601038

End: 4601739

Strand: Reverse

Name: phoB [H]

Synonym: AZC_4032

Alternate gene names: 158425656

Gene position: 4601739-4601038 (Counterclockwise)

Preceding gene: 158425657

Following gene: 158425655

Centisome position: 85.7

GC content: 68.8

Gene sequence:

>702_bases
GTGCCTGCGCGCATTCTGATCATCGAGGACGAGGAGCCGCTGACGCTCCTCCTGCGGTACAACCTCGAGAGCGAGGGTTA
CGCCGTCGACAATGTGGGGCGGGGTGATGAAGCCGAGACGCGGCTGCGCGAGAGCGTGCCCGATCTCGTCATCCTCGACT
GGATGCTGCCGGGCCTTTCGGGCATCGAGCTCTGTCGCCGGCTGCGCACGCGGCCGGAGACGGAGCGCCTGCCTGTCATC
ATGCTGACCGCGCGCGGCGAGGAGACCGAGCGGGTGCGCGGCCTTGCCACGGGCGCCGACGATTATGTGGTGAAGCCCTT
CTCCGTGCCGGAATTGCTGGCGCGCGTGCGGGCGCTGCTGCGCCGGGCCAAGCCCGAGCATGTGGCGACCCTGCTGCGGG
CCGGCGACATCGAACTCGACCGCGAGACCCATCGGGTCCACCGCTCGGGCCGCGAGGTGCATCTCGGTCCGACCGAGTTC
CGCCTGCTCGAGTTCCTGATGCAGAGCCCTGGCCGCGTCTTCTCCCGCGAGCAGTTGCTCGACGGGGTGTGGGGCCAGGA
CGTTTATATCGACGAGCGCACCGTGGACGTGCACGTGGGCCGCCTGCGCAAGGCGATCAATCGCGGCCGCCAGCCCGATC
CCATCCGCACCGTGCGCGGCGCCGGTTATTCCTTCAACGAAATGTTCGCCCGCGCCCATTGA

Upstream 100 bases:

>100_bases
GGTCACCGGCAACATGCTCTCCGACGACCGCCCGAAGGCGGATGCGTCCAGCGAGACCACGGTGACCTTCCCCACCCGCA
CCCTTCAGGGGTAAGCCAAC

Downstream 100 bases:

>100_bases
GGGGCGCGGTGCGCCACGCCATCTGCCGTCCGATGGCCGCGTGGGTGGCGCGAGGGAGGCTGAGCATGGCAGGTCTGGTG
CGCATGTCGGGTCGCGTCCT

Product: phosphate regulon transcriptional regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MPARILIIEDEEPLTLLLRYNLESEGYAVDNVGRGDEAETRLRESVPDLVILDWMLPGLSGIELCRRLRTRPETERLPVI
MLTARGEETERVRGLATGADDYVVKPFSVPELLARVRALLRRAKPEHVATLLRAGDIELDRETHRVHRSGREVHLGPTEF
RLLEFLMQSPGRVFSREQLLDGVWGQDVYIDERTVDVHVGRLRKAINRGRQPDPIRTVRGAGYSFNEMFARAH

Sequences:

>Translated_233_residues
MPARILIIEDEEPLTLLLRYNLESEGYAVDNVGRGDEAETRLRESVPDLVILDWMLPGLSGIELCRRLRTRPETERLPVI
MLTARGEETERVRGLATGADDYVVKPFSVPELLARVRALLRRAKPEHVATLLRAGDIELDRETHRVHRSGREVHLGPTEF
RLLEFLMQSPGRVFSREQLLDGVWGQDVYIDERTVDVHVGRLRKAINRGRQPDPIRTVRGAGYSFNEMFARAH
>Mature_232_residues
PARILIIEDEEPLTLLLRYNLESEGYAVDNVGRGDEAETRLRESVPDLVILDWMLPGLSGIELCRRLRTRPETERLPVIM
LTARGEETERVRGLATGADDYVVKPFSVPELLARVRALLRRAKPEHVATLLRAGDIELDRETHRVHRSGREVHLGPTEFR
LLEFLMQSPGRVFSREQLLDGVWGQDVYIDERTVDVHVGRLRKAINRGRQPDPIRTVRGAGYSFNEMFARAH

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=48.9082969432314, Blast_Score=224, Evalue=4e-60,
Organism=Escherichia coli, GI1790860, Length=226, Percent_Identity=45.575221238938, Blast_Score=167, Evalue=4e-43,
Organism=Escherichia coli, GI1788394, Length=227, Percent_Identity=40.0881057268723, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=39.0909090909091, Blast_Score=150, Evalue=7e-38,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=36.2445414847162, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI87082012, Length=222, Percent_Identity=38.7387387387387, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786911, Length=235, Percent_Identity=39.5744680851064, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1789402, Length=224, Percent_Identity=38.3928571428571, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=39.5454545454545, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787375, Length=222, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI2367329, Length=228, Percent_Identity=37.280701754386, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1790863, Length=230, Percent_Identity=34.7826086956522, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=33.9130434782609, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI145693140, Length=234, Percent_Identity=32.9059829059829, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=35.7798165137615, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=27.5, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26664; Mature: 26533

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARILIIEDEEPLTLLLRYNLESEGYAVDNVGRGDEAETRLRESVPDLVILDWMLPGLS
CCEEEEEEECCCCEEEEEEECCCCCCEEECCCCCCCHHHHHHHHCCCCEEEHHHHHCCCC
GIELCRRLRTRPETERLPVIMLTARGEETERVRGLATGADDYVVKPFSVPELLARVRALL
HHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHH
RRAKPEHVATLLRAGDIELDRETHRVHRSGREVHLGPTEFRLLEFLMQSPGRVFSREQLL
HHCCHHHHHHHHHHCCCEECHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCCHHHHHHHH
DGVWGQDVYIDERTVDVHVGRLRKAINRGRQPDPIRTVRGAGYSFNEMFARAH
CCCCCCCEEEECCEEEHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHCCC
>Mature Secondary Structure 
PARILIIEDEEPLTLLLRYNLESEGYAVDNVGRGDEAETRLRESVPDLVILDWMLPGLS
CEEEEEEECCCCEEEEEEECCCCCCEEECCCCCCCHHHHHHHHCCCCEEEHHHHHCCCC
GIELCRRLRTRPETERLPVIMLTARGEETERVRGLATGADDYVVKPFSVPELLARVRALL
HHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHH
RRAKPEHVATLLRAGDIELDRETHRVHRSGREVHLGPTEFRLLEFLMQSPGRVFSREQLL
HHCCHHHHHHHHHHCCCEECHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCCHHHHHHHH
DGVWGQDVYIDERTVDVHVGRLRKAINRGRQPDPIRTVRGAGYSFNEMFARAH
CCCCCCCEEEECCEEEHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]