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The map label for this gene is pmrB [H]

Identifier: 158425610

GI number: 158425610

Start: 4552216

End: 4553568

Strand: Direct

Name: pmrB [H]

Synonym: AZC_3986

Alternate gene names: 158425610

Gene position: 4552216-4553568 (Clockwise)

Preceding gene: 158425609

Following gene: 158425611

Centisome position: 84.77

GC content: 69.18

Gene sequence:

>1353_bases
TTGAAACGGTTTCTGCCCGGCTCCATCGCCGCGTCGGGGGCGCTGTTTCTGGGGTTCTGCCTCACGGTCGTGCTGCTGTT
CTCCTGGGTCACCTTCGCCGCCCTGCAAGGGGATGACGGCGGCTATGCCCAGTTCGACGCGGCAACGGCCATCCAGAAGT
CCCTGAAGCGGACCGAGGCCGGCAGCTTCACCCTCCAGCCCGTGGAGGATCTGCTCGCCTATCAGCGGAAATTCCCCACC
CTCTGGTATCTCGTCTCCGACGGCCGGACGTCCGTGAGTTACGGGCCGGTGCCCGAGCGGGTGCAGGCCGGCGCCAAGGT
CTGGAGCCCGCCGCAGCCGCTCTCCGGCTACGCGGTCAACGGCTCGACCATGAGGACGGAGCGGCTGGGCCTCGTGGTGC
CGCTCGAGGACGACACCATCCTCATCGAAGTGGGCGGGGCGGCCTACAGCAGCACCCAGATCACCATCGAACTGCTGCGC
GACATCAACACCACGGCCGTGCCCATCCTCGTGGTGCTGTTCGCCACCATGGTCGTCACGGTCATCCTCGTGCCGCAGGT
CATCGCACGCCCCGTGCGCCGTGTGGCGACGGCGGCGGAGTTCATCGACGGCACGCGGGAAGGCACGCGTCTGCCGGAAG
ACAATGCGCCGACGGAACTGCGCCCGCTCATCGCCGCCTTCAACCGCGCGCTCGACCGCATCGACAACGTGACCGCCGAG
CAGCGCCGCTTCCTCTCCAACGCCGCCCACGAACTGCGCACGCCACTGGCCCGCGCGCGCACACGTCTCGAGGCCGTGCC
GGACCCGCGCCTGCGGGCGGAGCTTGCCACCGAGATCCATTCCCTCTCGGCCACCGTGACCATGCTGCTCCAGCTCGCCC
GGCTCACCTCCGAGCCCGCCGAGATCAGCCGCATCAACCTCACCGACCTCACGCGCAACCTGACGGCGCAGGAGGCGCCG
AGCCTCGTCGCCGCGGGAATGGAAGTGGAGTTCCAGGCGCCGGATACGCCCGTCTTCATCAACGGCTCGGCACAGGCCAT
CACCGTCGCGCTCTCCAACCTCATCCGCAACGCAGCCCAGCATGCGGCCTCCGGCGGTCGGGTGGTCGTGCAGGTGGAAG
CCTGCGGGCGCGTCTCGGTGATCGATTTCGGCGCCGGCATCGGCACGATCTACAACGGATCGAGCGTGATGCAGCCCTTC
GCCCGCAGCAGCCAGAGCAGCAGCGGCACGGGCCTCGGCCTCTCCATCGTCGCGCAGGTGGCGGCCCTTCATAATGCGGG
GGTTTCGCTGGACGAGACGCCCGGCGGCGGCACCACCGTGCGCATCGCCTTCACGCCGCTGCCGGTCGCCTGA

Upstream 100 bases:

>100_bases
TCGACGCCCATCTCTCGCGCCTGCGCAAGCGCCTGCGCGAGCACAACTGCCAGGCCCAGATCCGCCCCCTGCGCGGTCTG
GGCTACATGCTGTCCGAACA

Downstream 100 bases:

>100_bases
ACGGGCCCGATCCCGCGTAATGTCCGCGTAATCCTCACCGACAGAATGCCGGCACCAGCGCGCGAGCCCTGGGGCCGGAC
GCATGTCCTGTTGCCGGCCC

Product: periplasmic sensor signal transduction histidine kinase precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKRFLPGSIAASGALFLGFCLTVVLLFSWVTFAALQGDDGGYAQFDAATAIQKSLKRTEAGSFTLQPVEDLLAYQRKFPT
LWYLVSDGRTSVSYGPVPERVQAGAKVWSPPQPLSGYAVNGSTMRTERLGLVVPLEDDTILIEVGGAAYSSTQITIELLR
DINTTAVPILVVLFATMVVTVILVPQVIARPVRRVATAAEFIDGTREGTRLPEDNAPTELRPLIAAFNRALDRIDNVTAE
QRRFLSNAAHELRTPLARARTRLEAVPDPRLRAELATEIHSLSATVTMLLQLARLTSEPAEISRINLTDLTRNLTAQEAP
SLVAAGMEVEFQAPDTPVFINGSAQAITVALSNLIRNAAQHAASGGRVVVQVEACGRVSVIDFGAGIGTIYNGSSVMQPF
ARSSQSSSGTGLGLSIVAQVAALHNAGVSLDETPGGGTTVRIAFTPLPVA

Sequences:

>Translated_450_residues
MKRFLPGSIAASGALFLGFCLTVVLLFSWVTFAALQGDDGGYAQFDAATAIQKSLKRTEAGSFTLQPVEDLLAYQRKFPT
LWYLVSDGRTSVSYGPVPERVQAGAKVWSPPQPLSGYAVNGSTMRTERLGLVVPLEDDTILIEVGGAAYSSTQITIELLR
DINTTAVPILVVLFATMVVTVILVPQVIARPVRRVATAAEFIDGTREGTRLPEDNAPTELRPLIAAFNRALDRIDNVTAE
QRRFLSNAAHELRTPLARARTRLEAVPDPRLRAELATEIHSLSATVTMLLQLARLTSEPAEISRINLTDLTRNLTAQEAP
SLVAAGMEVEFQAPDTPVFINGSAQAITVALSNLIRNAAQHAASGGRVVVQVEACGRVSVIDFGAGIGTIYNGSSVMQPF
ARSSQSSSGTGLGLSIVAQVAALHNAGVSLDETPGGGTTVRIAFTPLPVA
>Mature_450_residues
MKRFLPGSIAASGALFLGFCLTVVLLFSWVTFAALQGDDGGYAQFDAATAIQKSLKRTEAGSFTLQPVEDLLAYQRKFPT
LWYLVSDGRTSVSYGPVPERVQAGAKVWSPPQPLSGYAVNGSTMRTERLGLVVPLEDDTILIEVGGAAYSSTQITIELLR
DINTTAVPILVVLFATMVVTVILVPQVIARPVRRVATAAEFIDGTREGTRLPEDNAPTELRPLIAAFNRALDRIDNVTAE
QRRFLSNAAHELRTPLARARTRLEAVPDPRLRAELATEIHSLSATVTMLLQLARLTSEPAEISRINLTDLTRNLTAQEAP
SLVAAGMEVEFQAPDTPVFINGSAQAITVALSNLIRNAAQHAASGGRVVVQVEACGRVSVIDFGAGIGTIYNGSSVMQPF
ARSSQSSSGTGLGLSIVAQVAALHNAGVSLDETPGGGTTVRIAFTPLPVA

Specific function: Member of the two-component regulatory system pmrB/pmrA involved in regulation of virulence. Functions probably as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to pmrA in response to external

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1789403, Length=289, Percent_Identity=28.0276816608997, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1790346, Length=272, Percent_Identity=27.9411764705882, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787894, Length=366, Percent_Identity=27.3224043715847, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788393, Length=271, Percent_Identity=25.830258302583, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789808, Length=240, Percent_Identity=27.5, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790551, Length=301, Percent_Identity=25.9136212624585, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1786600, Length=233, Percent_Identity=25.3218884120172, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI145693157, Length=221, Percent_Identity=28.0542986425339, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786783, Length=298, Percent_Identity=24.1610738255034, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47829; Mature: 47829

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFLPGSIAASGALFLGFCLTVVLLFSWVTFAALQGDDGGYAQFDAATAIQKSLKRTEA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCC
GSFTLQPVEDLLAYQRKFPTLWYLVSDGRTSVSYGPVPERVQAGAKVWSPPQPLSGYAVN
CCEEECCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEC
GSTMRTERLGLVVPLEDDTILIEVGGAAYSSTQITIELLRDINTTAVPILVVLFATMVVT
CCCEEHHHCEEEEEECCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VILVPQVIARPVRRVATAAEFIDGTREGTRLPEDNAPTELRPLIAAFNRALDRIDNVTAE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHH
QRRFLSNAAHELRTPLARARTRLEAVPDPRLRAELATEIHSLSATVTMLLQLARLTSEPA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EISRINLTDLTRNLTAQEAPSLVAAGMEVEFQAPDTPVFINGSAQAITVALSNLIRNAAQ
HHEEEEHHHHHHHCCHHHCCHHHHCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHH
HAASGGRVVVQVEACGRVSVIDFGAGIGTIYNGSSVMQPFARSSQSSSGTGLGLSIVAQV
HHCCCCEEEEEEECCCCEEEEEECCCCCEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHH
AALHNAGVSLDETPGGGTTVRIAFTPLPVA
HHHHHCCCCCCCCCCCCCEEEEEEECCCCC
>Mature Secondary Structure
MKRFLPGSIAASGALFLGFCLTVVLLFSWVTFAALQGDDGGYAQFDAATAIQKSLKRTEA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCC
GSFTLQPVEDLLAYQRKFPTLWYLVSDGRTSVSYGPVPERVQAGAKVWSPPQPLSGYAVN
CCEEECCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEC
GSTMRTERLGLVVPLEDDTILIEVGGAAYSSTQITIELLRDINTTAVPILVVLFATMVVT
CCCEEHHHCEEEEEECCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VILVPQVIARPVRRVATAAEFIDGTREGTRLPEDNAPTELRPLIAAFNRALDRIDNVTAE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHH
QRRFLSNAAHELRTPLARARTRLEAVPDPRLRAELATEIHSLSATVTMLLQLARLTSEPA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EISRINLTDLTRNLTAQEAPSLVAAGMEVEFQAPDTPVFINGSAQAITVALSNLIRNAAQ
HHEEEEHHHHHHHCCHHHCCHHHHCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHH
HAASGGRVVVQVEACGRVSVIDFGAGIGTIYNGSSVMQPFARSSQSSSGTGLGLSIVAQV
HHCCCCEEEEEEECCCCEEEEEECCCCCEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHH
AALHNAGVSLDETPGGGTTVRIAFTPLPVA
HHHHHCCCCCCCCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14617142 [H]