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The map label for this gene is pleD [H]

Identifier: 158425594

GI number: 158425594

Start: 4537370

End: 4538926

Strand: Direct

Name: pleD [H]

Synonym: AZC_3970

Alternate gene names: 158425594

Gene position: 4537370-4538926 (Clockwise)

Preceding gene: 158425593

Following gene: 158425595

Centisome position: 84.5

GC content: 67.5

Gene sequence:

>1557_bases
ATGCTGAACCCCGATGTGCGACGCACCTATGCGCTGGCCGCGATCTGCGTTGGTGTTTTCCTCTCTTTGGCCGTCGCCGC
CTTCCTGCTCTGGGAAAACCGCCGTGAACGCTGGGCCAGCGCGGTGCAGTCCGCGCACAATGTGCTCATCACGCTCACCA
GCGACATCAACCGCAATCTGAACATCGTCGACCTGTCCCTCCAGGGGGCACAGGATGCCTTCCTCAAGGCCGACCTCACG
ACGCTGGACCCCGCCACGCGCAACATGGTGCTGTTCGACCGCGCCCTGAACGCGCGCTACCTCGGCGTCATGCTCATCCT
GTCGCCCAGCGGCGACGTGCTCTACGACCTCGGTCACCCCATTCCGCGTCCCGGTAATTTTTCCGATCGCGACTATTTCC
TGGTCCAGAAGGACGCGAATCACGCCCCCTATCTGAGCCGTCCGTACAACGCCCGTTTCCGCGACTTTGAACCGAGCGTC
GCTCTCAGCCGGCGCATCTCGGGGCCGGACGGGTCGTTCCGGGGCGTCGTCATGGGGGGCATCGGCCTCGAATTCTTCCG
CGATCTCTTCGCCAATGTGGATGTGGGTCCGCGCGGCATTGTCAGCCTGGTGCGGGACGACGGGGTGATCGTGATGCGCT
GGCCCTCCAGCGATGGGCACGGCGACACCAACCTCGACATCGCCAGCTCCGAAGGGATGCGACGGGTCATGACCGGCGAC
GGCCAGCCCTTCACCATGGACGGGGCCGTGGACGGGGTGCGCCGGCACTATTTCTACGACCGGGTCGGCCAGTATCCGCT
CCGCCTCGTCGTAGCCTTCGCGGTGGATGACGTACTCGCGCCCTGGCGCCGCCAGGCCCTCGCCTTCGGCGCCATCAGCG
GCGCATCGAGCCTGCTGATCATCCTGCTGGTGATCGCCTTGCAGCGGGCGCTGCGCAAGGAGCGGCTCGTCTCCGCCCGG
CTGGAAAACCTCGCCACGACCGATTGGCTCACCGGGCTCGCCAACCGGCGCAGCTTCGACGAAAATCTCATCATGGCCGG
TCGCCGGGCCGCCCGTTCCGGCGCGTCCCTTTCGCTGCTGATGATTGATGCCGACCGCTTCAAGCTTCTCAACGACACCT
TCGGCCACGCCAAGGGCGACGAGGTGCTGCACCAGATCGGTGCCGTCATCGCCGCCGCCGCCCGGCGGGCCGATGATGTG
GCGGCGCGCTATGGCGGGGAGGAATTCGCCGTCATCCTGCCCGCCACCGGAGCCGCCGGAGCGGCCCGTGTGGCCGAAGG
CATCCGCGCCGACATCGAACGGCAGGTCGGCCGCCCGAATATGCCGGTGAGCGTCAGCATCGGCGTCGCCACCGGGCACC
TCCTCACCGGGCAGGATGCGGAAGGGCTGCTCGCCGCGGCGGACAAGGCCGTCTACGAAGCAAAGGCGAATGGTCGCAAC
CGCGTGGCGGTGCGCAACCTCACCGAGACGCCGGATCACCGCCGTCCTCCGGATGCCGGCACTCACGCGGCCGCCGACTA
CACGTCCGAGGATCCCCGGACCGCTCAGGCGGTCTGA

Upstream 100 bases:

>100_bases
CGGAAAAATGCGGCGCCGCTCTAGATTTCGCTGCCGCACAATGCAACGCATGGTATGTTCGCATGCGAGTGAAATGCACG
AGTCCGGACCGCCATAAGGC

Downstream 100 bases:

>100_bases
TCCATCGCAAGGCGGAGCGTGCCTGCGGCGCTTTCCATCAGGGACAATCTTCGACACACCGGACTGGCCCTTGTGCCGCG
CATGCTATCCATCGCATGGT

Product: two component system fusin protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MLNPDVRRTYALAAICVGVFLSLAVAAFLLWENRRERWASAVQSAHNVLITLTSDINRNLNIVDLSLQGAQDAFLKADLT
TLDPATRNMVLFDRALNARYLGVMLILSPSGDVLYDLGHPIPRPGNFSDRDYFLVQKDANHAPYLSRPYNARFRDFEPSV
ALSRRISGPDGSFRGVVMGGIGLEFFRDLFANVDVGPRGIVSLVRDDGVIVMRWPSSDGHGDTNLDIASSEGMRRVMTGD
GQPFTMDGAVDGVRRHYFYDRVGQYPLRLVVAFAVDDVLAPWRRQALAFGAISGASSLLIILLVIALQRALRKERLVSAR
LENLATTDWLTGLANRRSFDENLIMAGRRAARSGASLSLLMIDADRFKLLNDTFGHAKGDEVLHQIGAVIAAAARRADDV
AARYGGEEFAVILPATGAAGAARVAEGIRADIERQVGRPNMPVSVSIGVATGHLLTGQDAEGLLAAADKAVYEAKANGRN
RVAVRNLTETPDHRRPPDAGTHAAADYTSEDPRTAQAV

Sequences:

>Translated_518_residues
MLNPDVRRTYALAAICVGVFLSLAVAAFLLWENRRERWASAVQSAHNVLITLTSDINRNLNIVDLSLQGAQDAFLKADLT
TLDPATRNMVLFDRALNARYLGVMLILSPSGDVLYDLGHPIPRPGNFSDRDYFLVQKDANHAPYLSRPYNARFRDFEPSV
ALSRRISGPDGSFRGVVMGGIGLEFFRDLFANVDVGPRGIVSLVRDDGVIVMRWPSSDGHGDTNLDIASSEGMRRVMTGD
GQPFTMDGAVDGVRRHYFYDRVGQYPLRLVVAFAVDDVLAPWRRQALAFGAISGASSLLIILLVIALQRALRKERLVSAR
LENLATTDWLTGLANRRSFDENLIMAGRRAARSGASLSLLMIDADRFKLLNDTFGHAKGDEVLHQIGAVIAAAARRADDV
AARYGGEEFAVILPATGAAGAARVAEGIRADIERQVGRPNMPVSVSIGVATGHLLTGQDAEGLLAAADKAVYEAKANGRN
RVAVRNLTETPDHRRPPDAGTHAAADYTSEDPRTAQAV
>Mature_518_residues
MLNPDVRRTYALAAICVGVFLSLAVAAFLLWENRRERWASAVQSAHNVLITLTSDINRNLNIVDLSLQGAQDAFLKADLT
TLDPATRNMVLFDRALNARYLGVMLILSPSGDVLYDLGHPIPRPGNFSDRDYFLVQKDANHAPYLSRPYNARFRDFEPSV
ALSRRISGPDGSFRGVVMGGIGLEFFRDLFANVDVGPRGIVSLVRDDGVIVMRWPSSDGHGDTNLDIASSEGMRRVMTGD
GQPFTMDGAVDGVRRHYFYDRVGQYPLRLVVAFAVDDVLAPWRRQALAFGAISGASSLLIILLVIALQRALRKERLVSAR
LENLATTDWLTGLANRRSFDENLIMAGRRAARSGASLSLLMIDADRFKLLNDTFGHAKGDEVLHQIGAVIAAAARRADDV
AARYGGEEFAVILPATGAAGAARVAEGIRADIERQVGRPNMPVSVSIGVATGHLLTGQDAEGLLAAADKAVYEAKANGRN
RVAVRNLTETPDHRRPPDAGTHAAADYTSEDPRTAQAV

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=181, Percent_Identity=37.5690607734807, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787262, Length=177, Percent_Identity=35.5932203389831, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI87082007, Length=205, Percent_Identity=35.609756097561, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI145693134, Length=163, Percent_Identity=39.2638036809816, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=37.2781065088757, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1787056, Length=287, Percent_Identity=30.3135888501742, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787802, Length=171, Percent_Identity=32.1637426900585, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788381, Length=208, Percent_Identity=31.7307692307692, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788085, Length=172, Percent_Identity=30.2325581395349, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787541, Length=178, Percent_Identity=32.0224719101124, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081974, Length=196, Percent_Identity=29.5918367346939, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI87081977, Length=179, Percent_Identity=33.5195530726257, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788956, Length=167, Percent_Identity=33.5329341317365, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 56238; Mature: 56238

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNPDVRRTYALAAICVGVFLSLAVAAFLLWENRRERWASAVQSAHNVLITLTSDINRNL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCC
NIVDLSLQGAQDAFLKADLTTLDPATRNMVLFDRALNARYLGVMLILSPSGDVLYDLGHP
EEEEEEECCCCHHHHCCCCCCCCCCCCCEEEEEHHCCCEEEEEEEEECCCCCEEEECCCC
IPRPGNFSDRDYFLVQKDANHAPYLSRPYNARFRDFEPSVALSRRISGPDGSFRGVVMGG
CCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEC
IGLEFFRDLFANVDVGPRGIVSLVRDDGVIVMRWPSSDGHGDTNLDIASSEGMRRVMTGD
CCHHHHHHHHHCCCCCHHHHHHHEECCCEEEEECCCCCCCCCCCEEEECCCCCHHHHCCC
GQPFTMDGAVDGVRRHYFYDRVGQYPLRLVVAFAVDDVLAPWRRQALAFGAISGASSLLI
CCEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ILLVIALQRALRKERLVSARLENLATTDWLTGLANRRSFDENLIMAGRRAARSGASLSLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCHHHHCCCCCEEEE
MIDADRFKLLNDTFGHAKGDEVLHQIGAVIAAAARRADDVAARYGGEEFAVILPATGAAG
EEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCH
AARVAEGIRADIERQVGRPNMPVSVSIGVATGHLLTGQDAEGLLAAADKAVYEAKANGRN
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECEEECCCCCCCHHHHHHHHHHHHCCCCCC
RVAVRNLTETPDHRRPPDAGTHAAADYTSEDPRTAQAV
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLNPDVRRTYALAAICVGVFLSLAVAAFLLWENRRERWASAVQSAHNVLITLTSDINRNL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCC
NIVDLSLQGAQDAFLKADLTTLDPATRNMVLFDRALNARYLGVMLILSPSGDVLYDLGHP
EEEEEEECCCCHHHHCCCCCCCCCCCCCEEEEEHHCCCEEEEEEEEECCCCCEEEECCCC
IPRPGNFSDRDYFLVQKDANHAPYLSRPYNARFRDFEPSVALSRRISGPDGSFRGVVMGG
CCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEC
IGLEFFRDLFANVDVGPRGIVSLVRDDGVIVMRWPSSDGHGDTNLDIASSEGMRRVMTGD
CCHHHHHHHHHCCCCCHHHHHHHEECCCEEEEECCCCCCCCCCCEEEECCCCCHHHHCCC
GQPFTMDGAVDGVRRHYFYDRVGQYPLRLVVAFAVDDVLAPWRRQALAFGAISGASSLLI
CCEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ILLVIALQRALRKERLVSARLENLATTDWLTGLANRRSFDENLIMAGRRAARSGASLSLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCHHHHCCCCCEEEE
MIDADRFKLLNDTFGHAKGDEVLHQIGAVIAAAARRADDVAARYGGEEFAVILPATGAAG
EEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCH
AARVAEGIRADIERQVGRPNMPVSVSIGVATGHLLTGQDAEGLLAAADKAVYEAKANGRN
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECEEECCCCCCCHHHHHHHHHHHHCCCCCC
RVAVRNLTETPDHRRPPDAGTHAAADYTSEDPRTAQAV
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA