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The map label for this gene is cbiA [H]

Identifier: 158425570

GI number: 158425570

Start: 4510910

End: 4512226

Strand: Direct

Name: cbiA [H]

Synonym: AZC_3946

Alternate gene names: 158425570

Gene position: 4510910-4512226 (Clockwise)

Preceding gene: 158425568

Following gene: 158425571

Centisome position: 84.01

GC content: 72.89

Gene sequence:

>1317_bases
ATGAGCCTCAACGGACTGCTCATCGGCGCCCCGCGCTCGGGCTCCGGCAAGACCACGGTGACGCTCGGCCTCCTCGCCGC
GCTCGCCCGGCGTGGCGTTGCCGTGCGGGCGGCGAAATCGGGTCCGGACTATATCGACCCCGCCTTCCACGCCGCCGCCA
CCGGCCGGCCGGGCCTCAATCTCGACAGTTGGGCCATGCCACCCGGCCTGCTCGCCCATCTGGTGGATCTCGCCGGGGCG
GATGGCGACCTCCTCGTGGTGGAAGGCGCCATGGGCCTGTTCGATGGCGTTGCCGCCCCGCCCCGCCGCAGCGGCGCCGG
GGCGGACATGGCGGCGCTGCTTGGCATTCCCGTGCTGCTGGTGCTCGATGTCTCCGGCCAGTCGCAGACCGCCGCCGCCG
TCGCGCGCGGCTTCATGGCGCATGATCCGCAGGTGCGCATCGGCGGGGTGATTCTCAACCGGGTGGGCAGCGAGCGGCAC
ATAAGGCTCGTGCGCGAGGCCATGGCGCCGCTCGGCCTGCCGGTCGTCGGCGCCATTCCGCGCGATACCAGCCTCGCGCT
GCCGGAGCGCCATCTCGGCCTCGTGCAGGCGGGCGAGCATCCCGATCTCGACGCGCGGCTCGATGCGCTGGCCGCCATGG
CGGAGGCCCATCTCGACCTCGACGCCATTCTGGGGCTCGCGGCCCCCGTGCGGCCGGTGGCCGCGCACCCGGCACGCGCC
CTGCCGCCGCCGGGGCAGCGGATCGCTCTGGCGCAGGATGCCGCCTTCACCTTCGTCTATGCCCATCTGCTTGCGGAATG
GCGGGCGGCGGGGGCGGAGATCCTCCCCTTCTCGCCGCTGGCAGACGAGGCGCCCGATCCTTCCGCCGACATCTGCTGGC
TGCCCGGCGGCTATCCCGAGCTGCATGGCGGGGCGCTCGCCAATGCCCAGCGCTTCAAGGCGGGGCTCACCGCCTTCGCA
GCGCACAAGCCGGTGCATGGGGAGTGCGGCGGCTACATGGCCCTCGGCCAGGGCATCGTCGATGCACAGGGCGCGCGCCA
CGCCATGGCCGGGCTGCTGGCGCTGGAGACGAGCTTCGCCAAGCGCAAAATGAATCTCGGCTACCGCGAGGCGTGCCTCC
TGAGCGACACGCCCATCGGCCCGGCGGGCACGCGGGTGCGGGGGCACGAATTCCATTATGCCAGCGTGATCGACCGGGGT
TCCGACGCGCCGCTGGCGGAGCTCGCCGACAGCCAGGGCACCGCGCTGCCGCCGTCGGGCAGCCGGCGCGGACAGGTGAC
GGGCACGTTCTTCCACGCCATTGCCCCTGCGGAATGA

Upstream 100 bases:

>100_bases
GCGCCGTCCGGCTGCTCCGCGAAAGGCCCGTCTTCCTTGCCGCCTGTGCTCATGAGGCCCTTCATAGGCCGCAGGCGGCG
CGCCGTCACGGGGGCCAGCC

Downstream 100 bases:

>100_bases
GCATCCGCTTTTTGCTGCATCGCAAAGCAAATCGCACCCTCCCGGCCCATGCTGGAGCGTGTCTTTTTGGACCGTTCCGG
CGTTATACTTGGGTCAACGA

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSLNGLLIGAPRSGSGKTTVTLGLLAALARRGVAVRAAKSGPDYIDPAFHAAATGRPGLNLDSWAMPPGLLAHLVDLAGA
DGDLLVVEGAMGLFDGVAAPPRRSGAGADMAALLGIPVLLVLDVSGQSQTAAAVARGFMAHDPQVRIGGVILNRVGSERH
IRLVREAMAPLGLPVVGAIPRDTSLALPERHLGLVQAGEHPDLDARLDALAAMAEAHLDLDAILGLAAPVRPVAAHPARA
LPPPGQRIALAQDAAFTFVYAHLLAEWRAAGAEILPFSPLADEAPDPSADICWLPGGYPELHGGALANAQRFKAGLTAFA
AHKPVHGECGGYMALGQGIVDAQGARHAMAGLLALETSFAKRKMNLGYREACLLSDTPIGPAGTRVRGHEFHYASVIDRG
SDAPLAELADSQGTALPPSGSRRGQVTGTFFHAIAPAE

Sequences:

>Translated_438_residues
MSLNGLLIGAPRSGSGKTTVTLGLLAALARRGVAVRAAKSGPDYIDPAFHAAATGRPGLNLDSWAMPPGLLAHLVDLAGA
DGDLLVVEGAMGLFDGVAAPPRRSGAGADMAALLGIPVLLVLDVSGQSQTAAAVARGFMAHDPQVRIGGVILNRVGSERH
IRLVREAMAPLGLPVVGAIPRDTSLALPERHLGLVQAGEHPDLDARLDALAAMAEAHLDLDAILGLAAPVRPVAAHPARA
LPPPGQRIALAQDAAFTFVYAHLLAEWRAAGAEILPFSPLADEAPDPSADICWLPGGYPELHGGALANAQRFKAGLTAFA
AHKPVHGECGGYMALGQGIVDAQGARHAMAGLLALETSFAKRKMNLGYREACLLSDTPIGPAGTRVRGHEFHYASVIDRG
SDAPLAELADSQGTALPPSGSRRGQVTGTFFHAIAPAE
>Mature_437_residues
SLNGLLIGAPRSGSGKTTVTLGLLAALARRGVAVRAAKSGPDYIDPAFHAAATGRPGLNLDSWAMPPGLLAHLVDLAGAD
GDLLVVEGAMGLFDGVAAPPRRSGAGADMAALLGIPVLLVLDVSGQSQTAAAVARGFMAHDPQVRIGGVILNRVGSERHI
RLVREAMAPLGLPVVGAIPRDTSLALPERHLGLVQAGEHPDLDARLDALAAMAEAHLDLDAILGLAAPVRPVAAHPARAL
PPPGQRIALAQDAAFTFVYAHLLAEWRAAGAEILPFSPLADEAPDPSADICWLPGGYPELHGGALANAQRFKAGLTAFAA
HKPVHGECGGYMALGQGIVDAQGARHAMAGLLALETSFAKRKMNLGYREACLLSDTPIGPAGTRVRGHEFHYASVIDRGS
DAPLAELADSQGTALPPSGSRRGQVTGTFFHAIAPAE

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 45010; Mature: 44879

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNGLLIGAPRSGSGKTTVTLGLLAALARRGVAVRAAKSGPDYIDPAFHAAATGRPGLN
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHCCCCCCCC
LDSWAMPPGLLAHLVDLAGADGDLLVVEGAMGLFDGVAAPPRRSGAGADMAALLGIPVLL
CCCCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCCCCCCCCCCHHHHHHCCCEEE
VLDVSGQSQTAAAVARGFMAHDPQVRIGGVILNRVGSERHIRLVREAMAPLGLPVVGAIP
EEECCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHCCCHHHHHHHHHHHHCCCCCEEECCC
RDTSLALPERHLGLVQAGEHPDLDARLDALAAMAEAHLDLDAILGLAAPVRPVAAHPARA
CCCCCCCCHHHCCCEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHCCCCCC
LPPPGQRIALAQDAAFTFVYAHLLAEWRAAGAEILPFSPLADEAPDPSADICWLPGGYPE
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCC
LHGGALANAQRFKAGLTAFAAHKPVHGECGGYMALGQGIVDAQGARHAMAGLLALETSFA
CCCCHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHH
KRKMNLGYREACLLSDTPIGPAGTRVRGHEFHYASVIDRGSDAPLAELADSQGTALPPSG
HHHHCCCHHHEEEECCCCCCCCCCCCCCCCEEEHHHHCCCCCCCHHHHCCCCCCCCCCCC
SRRGQVTGTFFHAIAPAE
CCCCCEEEEEEEEECCCC
>Mature Secondary Structure 
SLNGLLIGAPRSGSGKTTVTLGLLAALARRGVAVRAAKSGPDYIDPAFHAAATGRPGLN
CCCCEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHCCCCCCCC
LDSWAMPPGLLAHLVDLAGADGDLLVVEGAMGLFDGVAAPPRRSGAGADMAALLGIPVLL
CCCCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCCCCCCCCCCHHHHHHCCCEEE
VLDVSGQSQTAAAVARGFMAHDPQVRIGGVILNRVGSERHIRLVREAMAPLGLPVVGAIP
EEECCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHCCCHHHHHHHHHHHHCCCCCEEECCC
RDTSLALPERHLGLVQAGEHPDLDARLDALAAMAEAHLDLDAILGLAAPVRPVAAHPARA
CCCCCCCCHHHCCCEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHCCCCCC
LPPPGQRIALAQDAAFTFVYAHLLAEWRAAGAEILPFSPLADEAPDPSADICWLPGGYPE
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCC
LHGGALANAQRFKAGLTAFAAHKPVHGECGGYMALGQGIVDAQGARHAMAGLLALETSFA
CCCCHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHH
KRKMNLGYREACLLSDTPIGPAGTRVRGHEFHYASVIDRGSDAPLAELADSQGTALPPSG
HHHHCCCHHHEEEECCCCCCCCCCCCCCCCEEEHHHHCCCCCCCHHHHCCCCCCCCCCCC
SRRGQVTGTFFHAIAPAE
CCCCCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]