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The map label for this gene is ubiE [H]

Identifier: 158425543

GI number: 158425543

Start: 4480414

End: 4481184

Strand: Reverse

Name: ubiE [H]

Synonym: AZC_3919

Alternate gene names: 158425543

Gene position: 4481184-4480414 (Counterclockwise)

Preceding gene: 158425544

Following gene: 158425542

Centisome position: 83.45

GC content: 66.15

Gene sequence:

>771_bases
ATGCAACGCTCCGATACCGACGCGGCCGAAACCCATTTCGGCTACCGCACCGTGCCCTTGGGCGAGAAGCAGACCCTGGT
GGACGACGTCTTCCACAAGGTCGCCAAGCGCTACGACATCATGAACGACCTCATGTCCGGCGGCCTGCACCGGGCGTGGA
AGAATGTCCTCATCTCGAAGCTCCGCCCGCCGCAGGGCGACCGGCCCTTCCGCCTGCTGGATCTGGCTGGCGGCACCGGG
GATGTGTCCTTTCGCGCCGTGACGGCGGGCGGCACCGGTACGCGGGCCGTGGTGGCGGACATCAACACCGAGATGCTGGC
GGTGGGGCGCGAGCGCGCCGAGAAGCGCGGCCTCTCCGACAAGGTGGATTTCCGCGAGGCCAATGCGGAAGAACTGCCCT
TCGAGGATGCGAGCTTCGATGCCGTGACCATCGCGTTTGGCATCCGCAACGTGCCGCGCATTCCGCTTGCCCTCTCCGAG
ATGCGGCGGGTTCTGAAGCCGGGCGGGCAGGCGCTGGTGCTGGAATTCTCCAAGGTCGACGTGCCGCTGCTCGACAAGAT
CTACGAATTCTATTCCTTCCGGGTGATCCCGGAACTGGGCAAGCGGGTCGCGGGCGATGGCGAGCCCTATCAGTATCTGG
TGGAATCCATCCGCCGCTTCCCCGCGCCGGACGTCTTCGCGCAGATGATGCGCACGGCGGGCTTCGCGCGGGTGACCGCG
ACCCCGCTCACCGGCGGTATTGTCTGCCTCCATTCGGGTTACCGCATCTGA

Upstream 100 bases:

>100_bases
GGCTGCGGGCTGGTGCTGCTGCAATAGGGCACTGCGCCGCTTGGCGTCCCGCGACGCGATCCGATAAGGTCGCGCCGAAA
CTGATCCGCCGGGACGCACC

Downstream 100 bases:

>100_bases
TGGCCGTGCGCGGCCATCGCGGAAAGGGACCGGCGTGATTCTCGTTCTCATCGACGCCGCGCGCCTCGCGCGCGCCGGCT
ATGTGCTTGCCCGCGAGGGC

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MQRSDTDAAETHFGYRTVPLGEKQTLVDDVFHKVAKRYDIMNDLMSGGLHRAWKNVLISKLRPPQGDRPFRLLDLAGGTG
DVSFRAVTAGGTGTRAVVADINTEMLAVGRERAEKRGLSDKVDFREANAEELPFEDASFDAVTIAFGIRNVPRIPLALSE
MRRVLKPGGQALVLEFSKVDVPLLDKIYEFYSFRVIPELGKRVAGDGEPYQYLVESIRRFPAPDVFAQMMRTAGFARVTA
TPLTGGIVCLHSGYRI

Sequences:

>Translated_256_residues
MQRSDTDAAETHFGYRTVPLGEKQTLVDDVFHKVAKRYDIMNDLMSGGLHRAWKNVLISKLRPPQGDRPFRLLDLAGGTG
DVSFRAVTAGGTGTRAVVADINTEMLAVGRERAEKRGLSDKVDFREANAEELPFEDASFDAVTIAFGIRNVPRIPLALSE
MRRVLKPGGQALVLEFSKVDVPLLDKIYEFYSFRVIPELGKRVAGDGEPYQYLVESIRRFPAPDVFAQMMRTAGFARVTA
TPLTGGIVCLHSGYRI
>Mature_256_residues
MQRSDTDAAETHFGYRTVPLGEKQTLVDDVFHKVAKRYDIMNDLMSGGLHRAWKNVLISKLRPPQGDRPFRLLDLAGGTG
DVSFRAVTAGGTGTRAVVADINTEMLAVGRERAEKRGLSDKVDFREANAEELPFEDASFDAVTIAFGIRNVPRIPLALSE
MRRVLKPGGQALVLEFSKVDVPLLDKIYEFYSFRVIPELGKRVAGDGEPYQYLVESIRRFPAPDVFAQMMRTAGFARVTA
TPLTGGIVCLHSGYRI

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=282, Percent_Identity=47.5177304964539, Blast_Score=256, Evalue=2e-68,
Organism=Escherichia coli, GI48994983, Length=253, Percent_Identity=48.2213438735178, Blast_Score=228, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17557039, Length=248, Percent_Identity=46.3709677419355, Blast_Score=213, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI25143945, Length=110, Percent_Identity=29.0909090909091, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323526, Length=258, Percent_Identity=43.0232558139535, Blast_Score=196, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24641702, Length=259, Percent_Identity=47.4903474903475, Blast_Score=242, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.-

Molecular weight: Translated: 28362; Mature: 28362

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS01183 UBIE_1 ; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRSDTDAAETHFGYRTVPLGEKQTLVDDVFHKVAKRYDIMNDLMSGGLHRAWKNVLISK
CCCCCCCHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LRPPQGDRPFRLLDLAGGTGDVSFRAVTAGGTGTRAVVADINTEMLAVGRERAEKRGLSD
CCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHCCCCC
KVDFREANAEELPFEDASFDAVTIAFGIRNVPRIPLALSEMRRVLKPGGQALVLEFSKVD
CCCHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCC
VPLLDKIYEFYSFRVIPELGKRVAGDGEPYQYLVESIRRFPAPDVFAQMMRTAGFARVTA
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEE
TPLTGGIVCLHSGYRI
CCCCCCEEEECCCCCC
>Mature Secondary Structure
MQRSDTDAAETHFGYRTVPLGEKQTLVDDVFHKVAKRYDIMNDLMSGGLHRAWKNVLISK
CCCCCCCHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LRPPQGDRPFRLLDLAGGTGDVSFRAVTAGGTGTRAVVADINTEMLAVGRERAEKRGLSD
CCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHCCCCC
KVDFREANAEELPFEDASFDAVTIAFGIRNVPRIPLALSEMRRVLKPGGQALVLEFSKVD
CCCHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCC
VPLLDKIYEFYSFRVIPELGKRVAGDGEPYQYLVESIRRFPAPDVFAQMMRTAGFARVTA
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEE
TPLTGGIVCLHSGYRI
CCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]