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The map label for this gene is tam [H]

Identifier: 158425413

GI number: 158425413

Start: 4342663

End: 4343436

Strand: Reverse

Name: tam [H]

Synonym: AZC_3789

Alternate gene names: 158425413

Gene position: 4343436-4342663 (Counterclockwise)

Preceding gene: 158425414

Following gene: 158425394

Centisome position: 80.89

GC content: 66.02

Gene sequence:

>774_bases
ATGATCGCGGAATGGAGTGCGCGGCTTTATCTGACGTTCGAGGACGAACGCACCCGTCCGGCCCGTGACCTTGTTGCGCA
GATCCCTCTGGAAGCCGCGCGCCGCGTCGTCGATCTGGGATGCGGGCCCGGCAATTCCACCGAAATTCTGGCGGCCCGTT
ATCCCGAAGCCGATGTGATCGGCATCGACAGCTCTCCCGATATGCTCGCTGCCGCCCGCCGGCGCATGCCACACACGAGC
TTCATCCAGGCGGACGTGCGCACCTACCGTCTGCCGGAGCCGGCGGACGTGATACTGGCCAATGCCGTACTGAACTGGAT
GCCGGACCATCGCACGCTGTTTCCGCGTCTGGTCTCGCAACTGAGCGACGGCGGGGTTCTCGCCATCCAGTTGCCCGACA
ATCTGTCGGAGCCGGTGCACGAGACGCTGCGCGCGATGGCGGAGGGCGCCTGGGCGCGGCAGCTCAGCGCGGTACCCCGC
GCGCGCATTCCGCTGCTTCGCCCCGAGGAATATTACGATCTGCTGAGCCCCATCGCCGGCAAGGTGGAGATGTGGCGTAC
GGTCTATTGCCATCCGCTCGCCGATCATGCGGCGCTCATGGAGTGGATGCGGGGCGGAGGCGGCCTCCAGCCCTATCTCG
ATGCGCTGGCGCCTGCCGAGCGTCCGGATTTTCTCGCCGCCTATGAGGAGCGTCTCGCCCGCGTCTATCCCCGGCGTGCG
GATGGCCGCGTGCTGCTGTATTTCCCCCGTCTTTTTATCGTCGTGCTGCGCTGA

Upstream 100 bases:

>100_bases
CGCCGGATACTCCCCGGCGGGCGTTTTCGCAGGCGCCGCAGGGCTGCGCATGGCGCGTCCGCGGCGTGCTGTCGGCATGC
CCACAGCCGGGGGGGCGGCC

Downstream 100 bases:

>100_bases
GCCATCCCGACAGCGCCGTCCCGCGCGCTGGTTTTTCAGGGATGGTCGCCTCCTTCTTCCTCTTCCCCGTCACCGTTCGA
AGTTCTGCAGGCCAAGGGCC

Product: trans-aconitate methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MIAEWSARLYLTFEDERTRPARDLVAQIPLEAARRVVDLGCGPGNSTEILAARYPEADVIGIDSSPDMLAAARRRMPHTS
FIQADVRTYRLPEPADVILANAVLNWMPDHRTLFPRLVSQLSDGGVLAIQLPDNLSEPVHETLRAMAEGAWARQLSAVPR
ARIPLLRPEEYYDLLSPIAGKVEMWRTVYCHPLADHAALMEWMRGGGGLQPYLDALAPAERPDFLAAYEERLARVYPRRA
DGRVLLYFPRLFIVVLR

Sequences:

>Translated_257_residues
MIAEWSARLYLTFEDERTRPARDLVAQIPLEAARRVVDLGCGPGNSTEILAARYPEADVIGIDSSPDMLAAARRRMPHTS
FIQADVRTYRLPEPADVILANAVLNWMPDHRTLFPRLVSQLSDGGVLAIQLPDNLSEPVHETLRAMAEGAWARQLSAVPR
ARIPLLRPEEYYDLLSPIAGKVEMWRTVYCHPLADHAALMEWMRGGGGLQPYLDALAPAERPDFLAAYEERLARVYPRRA
DGRVLLYFPRLFIVVLR
>Mature_257_residues
MIAEWSARLYLTFEDERTRPARDLVAQIPLEAARRVVDLGCGPGNSTEILAARYPEADVIGIDSSPDMLAAARRRMPHTS
FIQADVRTYRLPEPADVILANAVLNWMPDHRTLFPRLVSQLSDGGVLAIQLPDNLSEPVHETLRAMAEGAWARQLSAVPR
ARIPLLRPEEYYDLLSPIAGKVEMWRTVYCHPLADHAALMEWMRGGGGLQPYLDALAPAERPDFLAAYEERLARVYPRRA
DGRVLLYFPRLFIVVLR

Specific function: Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate [H]

COG id: COG4106

COG function: function code R; Trans-aconitate methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Tam family [H]

Homologues:

Organism=Escherichia coli, GI1787798, Length=256, Percent_Identity=46.09375, Blast_Score=230, Evalue=6e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR023149 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.144 [H]

Molecular weight: Translated: 28964; Mature: 28964

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAEWSARLYLTFEDERTRPARDLVAQIPLEAARRVVDLGCGPGNSTEILAARYPEADVI
CCCCCCEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCEE
GIDSSPDMLAAARRRMPHTSFIQADVRTYRLPEPADVILANAVLNWMPDHRTLFPRLVSQ
ECCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
LSDGGVLAIQLPDNLSEPVHETLRAMAEGAWARQLSAVPRARIPLLRPEEYYDLLSPIAG
CCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHH
KVEMWRTVYCHPLADHAALMEWMRGGGGLQPYLDALAPAERPDFLAAYEERLARVYPRRA
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
DGRVLLYFPRLFIVVLR
CCEEEEEEHHHHHHCCC
>Mature Secondary Structure
MIAEWSARLYLTFEDERTRPARDLVAQIPLEAARRVVDLGCGPGNSTEILAARYPEADVI
CCCCCCEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCEE
GIDSSPDMLAAARRRMPHTSFIQADVRTYRLPEPADVILANAVLNWMPDHRTLFPRLVSQ
ECCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
LSDGGVLAIQLPDNLSEPVHETLRAMAEGAWARQLSAVPRARIPLLRPEEYYDLLSPIAG
CCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHH
KVEMWRTVYCHPLADHAALMEWMRGGGGLQPYLDALAPAERPDFLAAYEERLARVYPRRA
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
DGRVLLYFPRLFIVVLR
CCEEEEEEHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA