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The map label for this gene is tesA [H]

Identifier: 158425389

GI number: 158425389

Start: 4319804

End: 4320232

Strand: Direct

Name: tesA [H]

Synonym: AZC_3765

Alternate gene names: 158425389

Gene position: 4319804-4320232 (Clockwise)

Preceding gene: 158425385

Following gene: 158425390

Centisome position: 80.45

GC content: 64.8

Gene sequence:

>429_bases
GTGTCCGGCGACACCTCCACGCAGGGCCTCGCGCGGCTGGACTGGTCCGTACCACCCGGCACCGATGCAGTGATCGTCGA
GCTGGGCGCCAATGACATGCTGCGCGGACAGGATCCGAACATCACACGGCGCACGCTGGACGAGATCCTGACCCGGCTGA
AGGGCCGCAATATCCCCGTGCTGCTGGCCGGGATGCGGGCCGCGCCCAATTTCGGCGCCGACTACCAGAAGGCCTTTGAT
GCCATCTATCCCGATCTGGCGAGCAAGCACGGCGTGCTGCTCTATCCCTTCTTTCTCGATGGCGTTGCTGGAGATCGCAG
CCTCAATCAGGCCGACGGCATGCACCCCACGGCGGCCGGCGTGAACCGCATCGTGCCGGCCATTCTGCCCAGCGTCGAAA
AACTTCTGGCAGAGACCAGCCAACACTAG

Upstream 100 bases:

>100_bases
GACCGCCGGCTACGGCCTCCCGCGCGCCGATGCCTTTCCGGCGCGTCTTGAGGCGGCCCTGAGGGCCAAGGGATACGACG
TCTCCGTCACCAATGCGGGC

Downstream 100 bases:

>100_bases
TCAAAACCCCGAAATCATGCTCTTGGCGCGGGCGGGAAATCGAGGAAGCGCGCGCATTTTCGAACCATTTTGGTACCGAT
CGGGTGCAAGCCTCAGGAAG

Product: lysophospholipase L1

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 142; Mature: 141

Protein sequence:

>142_residues
MSGDTSTQGLARLDWSVPPGTDAVIVELGANDMLRGQDPNITRRTLDEILTRLKGRNIPVLLAGMRAAPNFGADYQKAFD
AIYPDLASKHGVLLYPFFLDGVAGDRSLNQADGMHPTAAGVNRIVPAILPSVEKLLAETSQH

Sequences:

>Translated_142_residues
MSGDTSTQGLARLDWSVPPGTDAVIVELGANDMLRGQDPNITRRTLDEILTRLKGRNIPVLLAGMRAAPNFGADYQKAFD
AIYPDLASKHGVLLYPFFLDGVAGDRSLNQADGMHPTAAGVNRIVPAILPSVEKLLAETSQH
>Mature_141_residues
SGDTSTQGLARLDWSVPPGTDAVIVELGANDMLRGQDPNITRRTLDEILTRLKGRNIPVLLAGMRAAPNFGADYQKAFDA
IYPDLASKHGVLLYPFFLDGVAGDRSLNQADGMHPTAAGVNRIVPAILPSVEKLLAETSQH

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=118, Percent_Identity=45.7627118644068, Blast_Score=91, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 15237; Mature: 15105

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGDTSTQGLARLDWSVPPGTDAVIVELGANDMLRGQDPNITRRTLDEILTRLKGRNIPV
CCCCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCE
LLAGMRAAPNFGADYQKAFDAIYPDLASKHGVLLYPFFLDGVAGDRSLNQADGMHPTAAG
EEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCCCCCCCCCCCCCHHH
VNRIVPAILPSVEKLLAETSQH
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGDTSTQGLARLDWSVPPGTDAVIVELGANDMLRGQDPNITRRTLDEILTRLKGRNIPV
CCCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCE
LLAGMRAAPNFGADYQKAFDAIYPDLASKHGVLLYPFFLDGVAGDRSLNQADGMHPTAAG
EEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCCCCCCCCCCCCCHHH
VNRIVPAILPSVEKLLAETSQH
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]