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The map label for this gene is ada [H]

Identifier: 158425306

GI number: 158425306

Start: 4229565

End: 4230536

Strand: Reverse

Name: ada [H]

Synonym: AZC_3682

Alternate gene names: 158425306

Gene position: 4230536-4229565 (Counterclockwise)

Preceding gene: 158425307

Following gene: 158425301

Centisome position: 78.78

GC content: 71.19

Gene sequence:

>972_bases
GTGATGGCACGAAACGTCCGAAGGGGAAGAACGCCCGTGCTCGACCTGACCGCCCAGACCACCCTGCCGCTTGAACTGCC
CCGCCCGCTGGCCGGCATGGTCGCAGGTCCGGCCGCCGCCCTCGACATCTGCGCCGCCGATTATGACGTGGTGCGCCGCG
CCGTCGCTTATGTGACCCATCACGTGCGCGACCAGCCCGAGGTGGAAGCCATCGCCGCCGCCGCGGGCGTGACGCCGCGC
GCGCTCACCGATCTTTTCCGCCGCTGGTGCGGCCTCACCCCCAAGGCGTTCCTTCAGGCGGTGACGCTGGACCGCGCCCG
GCAGGTGCTGGCCGAATGCCCGAACGTGCTCGATGCGGCGCTGGAGCTGGGCCTCTCCGGTCCCGGCCGCCTGCACGACC
TCTTCGTGGTGCATGAGGCCATGTCGCCGGGCGAGTGGAAGACGGGCGGTGCGGGGCTGGTCATCCGCTACGGCCTCCAT
CCCTCGCCCTTCGGCACGGCCCTCGCCATGTGTACGCCGCGCGGCCTCTGCGGCCTTGCCTTCGCGGATGCGGGCGAGGA
GGAGGCGGCGCTGGCCGACATGCGCCGGCGCTGGCCCTACGCCACGTATCTGGAGGACCCCACCGCCACCGCGCCGCTGG
TGTCGCGCATATTCAACCCCGAGGCGTGGCGGGAAGAGCAGCCGCTGCGCATCGTCTTCATCGGCACGGACTTCGAGATC
CGGGTGTGGGAGACGCTGCTGCGCATTCCCATGGGCCGGGCCACCACCTATTCGACCATAGCCGGACACGTGGGCAAGCC
CTCCGCCGCCCGCGCCGTGGGCGCGGCGGTGGGCAAGAACCCCATCTCCTTCGTGGTGCCCTGCCACCGCGTGCTCGGCA
AGAATGGCGATCTCACCGGCTATCACTGGGGCCTCACCCGCAAGCGCGCGATACTCGGCTGGGAGGCCGGCCGGATCGGG
ACGGAGGGCTGA

Upstream 100 bases:

>100_bases
CCCATGCCCTGAGCGAGGTGAAGCGCGGCGTCACCCGCACCCATTTCTGATCGGGGCGCGCGGCGGTGAAATCGGGTGGC
CCGCTGGCGCGGCCGGTGCT

Downstream 100 bases:

>100_bases
GACGCCTGCGTTGAAGTCGTAGGGTGCATCTTCCCCCTCCCCACCCCTCCCCCGCAGGCGGGAGAGGGGGCCCGTTGCGC
GGGCTGTTATGCACCGCATG

Product: methylated-DNA-(protein)-cysteine S-methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MMARNVRRGRTPVLDLTAQTTLPLELPRPLAGMVAGPAAALDICAADYDVVRRAVAYVTHHVRDQPEVEAIAAAAGVTPR
ALTDLFRRWCGLTPKAFLQAVTLDRARQVLAECPNVLDAALELGLSGPGRLHDLFVVHEAMSPGEWKTGGAGLVIRYGLH
PSPFGTALAMCTPRGLCGLAFADAGEEEAALADMRRRWPYATYLEDPTATAPLVSRIFNPEAWREEQPLRIVFIGTDFEI
RVWETLLRIPMGRATTYSTIAGHVGKPSAARAVGAAVGKNPISFVVPCHRVLGKNGDLTGYHWGLTRKRAILGWEAGRIG
TEG

Sequences:

>Translated_323_residues
MMARNVRRGRTPVLDLTAQTTLPLELPRPLAGMVAGPAAALDICAADYDVVRRAVAYVTHHVRDQPEVEAIAAAAGVTPR
ALTDLFRRWCGLTPKAFLQAVTLDRARQVLAECPNVLDAALELGLSGPGRLHDLFVVHEAMSPGEWKTGGAGLVIRYGLH
PSPFGTALAMCTPRGLCGLAFADAGEEEAALADMRRRWPYATYLEDPTATAPLVSRIFNPEAWREEQPLRIVFIGTDFEI
RVWETLLRIPMGRATTYSTIAGHVGKPSAARAVGAAVGKNPISFVVPCHRVLGKNGDLTGYHWGLTRKRAILGWEAGRIG
TEG
>Mature_323_residues
MMARNVRRGRTPVLDLTAQTTLPLELPRPLAGMVAGPAAALDICAADYDVVRRAVAYVTHHVRDQPEVEAIAAAAGVTPR
ALTDLFRRWCGLTPKAFLQAVTLDRARQVLAECPNVLDAALELGLSGPGRLHDLFVVHEAMSPGEWKTGGAGLVIRYGLH
PSPFGTALAMCTPRGLCGLAFADAGEEEAALADMRRRWPYATYLEDPTATAPLVSRIFNPEAWREEQPLRIVFIGTDFEI
RVWETLLRIPMGRATTYSTIAGHVGKPSAARAVGAAVGKNPISFVVPCHRVLGKNGDLTGYHWGLTRKRAILGWEAGRIG
TEG

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=116, Percent_Identity=37.0689655172414, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1788542, Length=252, Percent_Identity=30.5555555555556, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787596, Length=78, Percent_Identity=56.4102564102564, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI115533068, Length=84, Percent_Identity=42.8571428571429, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=84, Percent_Identity=42.8571428571429, Blast_Score=88, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=88, Percent_Identity=46.5909090909091, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17137554, Length=172, Percent_Identity=29.6511627906977, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 34915; Mature: 34915

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMARNVRRGRTPVLDLTAQTTLPLELPRPLAGMVAGPAAALDICAADYDVVRRAVAYVTH
CCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHH
HVRDQPEVEAIAAAAGVTPRALTDLFRRWCGLTPKAFLQAVTLDRARQVLAECPNVLDAA
HHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHH
LELGLSGPGRLHDLFVVHEAMSPGEWKTGGAGLVIRYGLHPSPFGTALAMCTPRGLCGLA
HHHCCCCCCCHHHHEEEEHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCEEH
FADAGEEEAALADMRRRWPYATYLEDPTATAPLVSRIFNPEAWREEQPLRIVFIGTDFEI
CCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHCCCHHCCCCCCEEEEEECCCCCH
RVWETLLRIPMGRATTYSTIAGHVGKPSAARAVGAAVGKNPISFVVPCHRVLGKNGDLTG
HHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCE
YHWGLTRKRAILGWEAGRIGTEG
EECCCCCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MMARNVRRGRTPVLDLTAQTTLPLELPRPLAGMVAGPAAALDICAADYDVVRRAVAYVTH
CCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHH
HVRDQPEVEAIAAAAGVTPRALTDLFRRWCGLTPKAFLQAVTLDRARQVLAECPNVLDAA
HHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHH
LELGLSGPGRLHDLFVVHEAMSPGEWKTGGAGLVIRYGLHPSPFGTALAMCTPRGLCGLA
HHHCCCCCCCHHHHEEEEHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCEEH
FADAGEEEAALADMRRRWPYATYLEDPTATAPLVSRIFNPEAWREEQPLRIVFIGTDFEI
CCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHCCCHHCCCCCCEEEEEECCCCCH
RVWETLLRIPMGRATTYSTIAGHVGKPSAARAVGAAVGKNPISFVVPCHRVLGKNGDLTG
HHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCE
YHWGLTRKRAILGWEAGRIGTEG
EECCCCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]