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The map label for this gene is exsB

Identifier: 158425299

GI number: 158425299

Start: 4221943

End: 4222668

Strand: Reverse

Name: exsB

Synonym: AZC_3675

Alternate gene names: 158425299

Gene position: 4222668-4221943 (Counterclockwise)

Preceding gene: 158425300

Following gene: 158425298

Centisome position: 78.64

GC content: 70.25

Gene sequence:

>726_bases
ATGAGTGCTGACGCGGGCCCGGGTTCGGGCGCGCTCGTCCTGTTCTCGGGCGGGCAGGATTCCACCACCACGCTCGCCTG
GGCGCTGGACCGCTTCGAGCGGGTGGAGACGCTCGGCTTCGACTATGGCCAGCGCCACCGCATCGAACTCGACTGCCGGG
TGAAGGTGCGCGCCGCCATGGCAGCGCTCAATCCGGCCTGGGCCGCGAAGCTGGGCGAGGACCATACCCTCGCGCTCGAT
GCGCTGGGCGCGGTCTCCGACACGGCGCTCACCCGCGACGTCGCCATCGAGATGACCGAGGGCGGCCTGCCGAACACCTT
CGTGCCCGGCCGCAACATCGTCTTCCTCACCTTCGCCGCCGCGCTCGCCTATCGGCGCGGGCTGAAGCACATCGTCGGCG
GCATGTGCGAGACCGACTTCTCCGGCTATCCGGACTGCCGGGACGACACCATCAAGGCCCTGCAGGTGGCGCTCAACCTC
GGCATGGACCGGCGCTTTGTGCTCGAGACACCGCTCATGTGGATCGACAAGGCCGAGACCTGGCGGCTCGCCGAGCGGCT
CGGCGGCCGGGCGCTGGTGGACCTCATCGTGGAGGACACCCATACCTGCTATCTTGGCGAACGCGGCCCGCGCCATCCGT
GGGGGCATGGCTGCGGGACCTGCCCGGCGTGCCAGCTGCGCGCCGAGGGCTTTGCCCGCTACGAGGCCGCGAGGGTGAGC
GAATGA

Upstream 100 bases:

>100_bases
AGAGCCGCATCGACCGCCACCGTCTGGTGAACGGGCTGCTGGCGGGCGAGATCGCCGGCGGTCTCCACGCGCTCGCCATC
GACGCCAAAGCGCCCGGCGA

Downstream 100 bases:

>100_bases
CACTGCGCCGCGCGTCCACTGCCCTGGCCCTCCTGGGTCTCGTTCTCGCCTTCTCACCTGCGCCCGCCGCCGCAGCCAGC
TTCGACTGCACCAAGGCCCG

Product: ExsB protein

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine synthase; PreQ(0) synthase; Queuosine biosynthesis protein queC

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MSADAGPGSGALVLFSGGQDSTTTLAWALDRFERVETLGFDYGQRHRIELDCRVKVRAAMAALNPAWAAKLGEDHTLALD
ALGAVSDTALTRDVAIEMTEGGLPNTFVPGRNIVFLTFAAALAYRRGLKHIVGGMCETDFSGYPDCRDDTIKALQVALNL
GMDRRFVLETPLMWIDKAETWRLAERLGGRALVDLIVEDTHTCYLGERGPRHPWGHGCGTCPACQLRAEGFARYEAARVS
E

Sequences:

>Translated_241_residues
MSADAGPGSGALVLFSGGQDSTTTLAWALDRFERVETLGFDYGQRHRIELDCRVKVRAAMAALNPAWAAKLGEDHTLALD
ALGAVSDTALTRDVAIEMTEGGLPNTFVPGRNIVFLTFAAALAYRRGLKHIVGGMCETDFSGYPDCRDDTIKALQVALNL
GMDRRFVLETPLMWIDKAETWRLAERLGGRALVDLIVEDTHTCYLGERGPRHPWGHGCGTCPACQLRAEGFARYEAARVS
E
>Mature_240_residues
SADAGPGSGALVLFSGGQDSTTTLAWALDRFERVETLGFDYGQRHRIELDCRVKVRAAMAALNPAWAAKLGEDHTLALDA
LGAVSDTALTRDVAIEMTEGGLPNTFVPGRNIVFLTFAAALAYRRGLKHIVGGMCETDFSGYPDCRDDTIKALQVALNLG
MDRRFVLETPLMWIDKAETWRLAERLGGRALVDLIVEDTHTCYLGERGPRHPWGHGCGTCPACQLRAEGFARYEAARVSE

Specific function: Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0))

COG id: COG0603

COG function: function code R; Predicted PP-loop superfamily ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queC family

Homologues:

Organism=Escherichia coli, GI1786648, Length=234, Percent_Identity=47.8632478632479, Blast_Score=199, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEC_AZOC5 (A8IMX7)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001526591.1
- ProteinModelPortal:   A8IMX7
- SMR:   A8IMX7
- GeneID:   5688524
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_3675
- HOGENOM:   HBG553284
- OMA:   CETDYSG
- ProtClustDB:   CLSK864419
- BioCyc:   ACAU438753:AZC_3675-MONOMER
- HAMAP:   MF_01633_B
- InterPro:   IPR018317
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF006293
- TIGRFAMs:   TIGR00364

Pfam domain/function: PF06508 ExsB

EC number: NA

Molecular weight: Translated: 26272; Mature: 26141

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADAGPGSGALVLFSGGQDSTTTLAWALDRFERVETLGFDYGQRHRIELDCRVKVRAAM
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEHHHHH
AALNPAWAAKLGEDHTLALDALGAVSDTALTRDVAIEMTEGGLPNTFVPGRNIVFLTFAA
HHCCHHHHHHCCCCCEEEHHHHCCHHHHHHHHEEEEEEECCCCCCCCCCCCCEEHHHHHH
ALAYRRGLKHIVGGMCETDFSGYPDCRDDTIKALQVALNLGMDRRFVLETPLMWIDKAET
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEECCCHH
WRLAERLGGRALVDLIVEDTHTCYLGERGPRHPWGHGCGTCPACQLRAEGFARYEAARVS
HHHHHHHCCHHEEHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
E
C
>Mature Secondary Structure 
SADAGPGSGALVLFSGGQDSTTTLAWALDRFERVETLGFDYGQRHRIELDCRVKVRAAM
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEHHHHH
AALNPAWAAKLGEDHTLALDALGAVSDTALTRDVAIEMTEGGLPNTFVPGRNIVFLTFAA
HHCCHHHHHHCCCCCEEEHHHHCCHHHHHHHHEEEEEEECCCCCCCCCCCCCEEHHHHHH
ALAYRRGLKHIVGGMCETDFSGYPDCRDDTIKALQVALNLGMDRRFVLETPLMWIDKAET
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEECCCHH
WRLAERLGGRALVDLIVEDTHTCYLGERGPRHPWGHGCGTCPACQLRAEGFARYEAARVS
HHHHHHHCCHHEEHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA