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The map label for this gene is dme [H]

Identifier: 158425280

GI number: 158425280

Start: 4199096

End: 4201375

Strand: Reverse

Name: dme [H]

Synonym: AZC_3656

Alternate gene names: 158425280

Gene position: 4201375-4199096 (Counterclockwise)

Preceding gene: 158425282

Following gene: 158425276

Centisome position: 78.24

GC content: 67.32

Gene sequence:

>2280_bases
ATGTCGAACATTTCCGAGGATCTGAAATCCGGCGCGCTTGTCTACCATCGCAGCCCCAAGCCTGGGAAACTGGAAATCCA
GGCGACCAAGCCCCTCGGCAATCAGCGCGATCTCGCTCTGGCCTATTCTCCCGGCGTCGCCGCGGCCTGCGAGGCCATCA
AGGCTGACCCGCTCCAGGCCGCAGAACTGACCACCCGCGCCAATCTTGTGGCGGTGGTGTCCAACGGCACCGCCGTGCTC
GGCCTCGGAAACATCGGCCCGCTCGCCTCCAAGCCCGTGATGGAGGGCAAGGCGGTCCTGTTCAAGAAGTTCGCCGGCAT
CGACGTGTTCGACATCGAGCTCGCCGCCACCGAGATCGACCATCTGGTCGAGACGGTGGCCGCGCTGGAGCCCACCTTCG
GCGGAATCAACCTTGAGGACATCAAGGCGCCCGAGTGCTTCGAGGTGGAGAAGCGCCTGCGCGAGCGCATGAAGATTCCG
GTCTTCCACGACGACCAGCATGGCACCGCCATCATCGTCGGCGCTGCCGTCCGCAACGCGCTGGAGATCGGCAACCGCAA
GATCGAAGACCTCAAGATCGTCACCTCCGGTGCGGGCGCGGCGGCCATCGCCTGCCTCAACCTGCTGGTGACCCTCGGCG
CCAAGCGCGAGAACATCTGGGTCTGCGACATCGAGGGCGTGGTCTATGAAGGCCGCGAGACCCTCATGGACCCCTACAAG
TCTGTCTATGCCCAGAAGACCGACGCCCGCACGCTCGCCGAGGTGATCGACGGCGCGCACATCTTCCTCGGCCTCTCCGC
CGGCGGCGTGCTGAAGCCGGAGATGGTCCAGCGCATGGCGGATCGTCCGCTCATCCTCGCGCTCGCCAACCCCAATCCCG
AGATCATGCCGGAAGAGGCCCGCGCCGTGCGGCCCGACGCCATGATCTGCACCGGCCGTTCGGACTTCCCGAACCAGGTG
AACAACGTCCTCTGCTTCCCCTACATCTTCCGCGGCGCGCTGGATGTGGGTGCCACCGACATCAATGCCGAGATGAAGAT
GGCGGCGGTGGAAGCCATCGCCGCGCTCGCCCGCGAGACGCCCTCCGACGTGGTGGCCCGCGCCTATGGCGGCGAGACCC
GCGCCTTCGGTGCGGACAGCATCATTCCTTCGCCGTTCGATCCGCGCCTCATCCTGCGCATCGCCCCGGCGGTGGCCAAG
GCGGCCATGGACACCGGCGTCGCCACCCGGCCCATCGCCGATTTCGACGCCTATAACGAGAAGCTCGACGAGTTCGTCTT
CCGCTCCGGCTTCATCATGCGTCCGCTGTTCCAGCGCGCGAAGCAGGACAAGAAGCGCGTCATCTACGCCGAGGGCGAGG
ACGAGCGCGTGCTGCGCGCCGCGCAGGCGGTGATCGAGGAGGGCATTGCCCATCCCATCCTCGTCGCCCGCCCGAGCGTG
CTCGAAGCCCGCCTCCAGCGCTTCGGTCTCTCGATCCGTCCCGGCAAGGACTTCGAGGTCATCAACCCCGAAGACGATCC
GCGCTACCGCGACTTCGTGCGCTCCTACATCGAGATCGCCGGCCGTCGCGGCGTGACGCCGGATGCCGCCCGCACGCTGG
TGCGCACCTCCTCGACCGTCATCTCGGCGCTGGCCGTGAAGAAGGGCGAGGCGGATGCCATGCTGTGCGGCATCGAAGGC
CGCTTCTCCCGCCATCTGCGTCATGTGCGCGACATCATCGGCCTGGCTCCGGGCGTGCGCGAACTGGCCGCGCTCTCGCT
GCTCATCACTCCGAAGGGCAATTTGTTCCTGTGCGACACGCAGGTGCAGACCGAGCCGAACGCCGCCGACCTTGCGGAGA
TGACCATCCTCGCCGCCGCGCACGTGCGGCGCTTCGGCATCGAGCCGAAGGTGGCCCTGCTGTCGCACTCCAATTTCGGC
AGCAACGACACCGTCTGCGCCCGCCGGGTCCGTGCGGCCCTCGACATCCTCAAGGACCGCGCGCCGGAGCTTGAGGTGGA
TGGCGAGATGCAGGCGGAACTCGCGCTGCTGCCGGATGCCCGCGAGCGCATTCTCCCGCACTCGCGCCTCCAGGGCGTGG
CCAACGTGCTGGTGATGCCCGACCTCGATGCGGCGGACATCGCCTACAACATGATCAAGGTGCTGGGCGACGCGCTCCCG
GTCGGCCCCATCCTCATGGGCACCGCCAAGCCGGCGCACATCCTCGGCCCGACGGTGACCGCCCGCGGCATCGTCAACAT
GACCGCCGTCGCCGTGGTGGAAGCCCAGAGCGAAGCCTGA

Upstream 100 bases:

>100_bases
GCCATGCCAGACCGTGACATCCCCCGCCGCGGGGGATAGGTTGCCCGCCCGTTCGGTGGACATCTGTAAGAGAGGCTTGG
GGGAGGACATGGCGTCAAAC

Downstream 100 bases:

>100_bases
TCCGCAAGGGCTGCCTTCCGGCGGCCCGGCACAAACAAAAGGCCCCGGCGCTTCTGGCGCCGGGGCCTTTTTGATTCGGG
AAGGTGCCGAGCCGGTCAGC

Product: malic enzyme

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 759; Mature: 758

Protein sequence:

>759_residues
MSNISEDLKSGALVYHRSPKPGKLEIQATKPLGNQRDLALAYSPGVAAACEAIKADPLQAAELTTRANLVAVVSNGTAVL
GLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIELAATEIDHLVETVAALEPTFGGINLEDIKAPECFEVEKRLRERMKIP
VFHDDQHGTAIIVGAAVRNALEIGNRKIEDLKIVTSGAGAAAIACLNLLVTLGAKRENIWVCDIEGVVYEGRETLMDPYK
SVYAQKTDARTLAEVIDGAHIFLGLSAGGVLKPEMVQRMADRPLILALANPNPEIMPEEARAVRPDAMICTGRSDFPNQV
NNVLCFPYIFRGALDVGATDINAEMKMAAVEAIAALARETPSDVVARAYGGETRAFGADSIIPSPFDPRLILRIAPAVAK
AAMDTGVATRPIADFDAYNEKLDEFVFRSGFIMRPLFQRAKQDKKRVIYAEGEDERVLRAAQAVIEEGIAHPILVARPSV
LEARLQRFGLSIRPGKDFEVINPEDDPRYRDFVRSYIEIAGRRGVTPDAARTLVRTSSTVISALAVKKGEADAMLCGIEG
RFSRHLRHVRDIIGLAPGVRELAALSLLITPKGNLFLCDTQVQTEPNAADLAEMTILAAAHVRRFGIEPKVALLSHSNFG
SNDTVCARRVRAALDILKDRAPELEVDGEMQAELALLPDARERILPHSRLQGVANVLVMPDLDAADIAYNMIKVLGDALP
VGPILMGTAKPAHILGPTVTARGIVNMTAVAVVEAQSEA

Sequences:

>Translated_759_residues
MSNISEDLKSGALVYHRSPKPGKLEIQATKPLGNQRDLALAYSPGVAAACEAIKADPLQAAELTTRANLVAVVSNGTAVL
GLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIELAATEIDHLVETVAALEPTFGGINLEDIKAPECFEVEKRLRERMKIP
VFHDDQHGTAIIVGAAVRNALEIGNRKIEDLKIVTSGAGAAAIACLNLLVTLGAKRENIWVCDIEGVVYEGRETLMDPYK
SVYAQKTDARTLAEVIDGAHIFLGLSAGGVLKPEMVQRMADRPLILALANPNPEIMPEEARAVRPDAMICTGRSDFPNQV
NNVLCFPYIFRGALDVGATDINAEMKMAAVEAIAALARETPSDVVARAYGGETRAFGADSIIPSPFDPRLILRIAPAVAK
AAMDTGVATRPIADFDAYNEKLDEFVFRSGFIMRPLFQRAKQDKKRVIYAEGEDERVLRAAQAVIEEGIAHPILVARPSV
LEARLQRFGLSIRPGKDFEVINPEDDPRYRDFVRSYIEIAGRRGVTPDAARTLVRTSSTVISALAVKKGEADAMLCGIEG
RFSRHLRHVRDIIGLAPGVRELAALSLLITPKGNLFLCDTQVQTEPNAADLAEMTILAAAHVRRFGIEPKVALLSHSNFG
SNDTVCARRVRAALDILKDRAPELEVDGEMQAELALLPDARERILPHSRLQGVANVLVMPDLDAADIAYNMIKVLGDALP
VGPILMGTAKPAHILGPTVTARGIVNMTAVAVVEAQSEA
>Mature_758_residues
SNISEDLKSGALVYHRSPKPGKLEIQATKPLGNQRDLALAYSPGVAAACEAIKADPLQAAELTTRANLVAVVSNGTAVLG
LGNIGPLASKPVMEGKAVLFKKFAGIDVFDIELAATEIDHLVETVAALEPTFGGINLEDIKAPECFEVEKRLRERMKIPV
FHDDQHGTAIIVGAAVRNALEIGNRKIEDLKIVTSGAGAAAIACLNLLVTLGAKRENIWVCDIEGVVYEGRETLMDPYKS
VYAQKTDARTLAEVIDGAHIFLGLSAGGVLKPEMVQRMADRPLILALANPNPEIMPEEARAVRPDAMICTGRSDFPNQVN
NVLCFPYIFRGALDVGATDINAEMKMAAVEAIAALARETPSDVVARAYGGETRAFGADSIIPSPFDPRLILRIAPAVAKA
AMDTGVATRPIADFDAYNEKLDEFVFRSGFIMRPLFQRAKQDKKRVIYAEGEDERVLRAAQAVIEEGIAHPILVARPSVL
EARLQRFGLSIRPGKDFEVINPEDDPRYRDFVRSYIEIAGRRGVTPDAARTLVRTSSTVISALAVKKGEADAMLCGIEGR
FSRHLRHVRDIIGLAPGVRELAALSLLITPKGNLFLCDTQVQTEPNAADLAEMTILAAAHVRRFGIEPKVALLSHSNFGS
NDTVCARRVRAALDILKDRAPELEVDGEMQAELALLPDARERILPHSRLQGVANVLVMPDLDAADIAYNMIKVLGDALPV
GPILMGTAKPAHILGPTVTARGIVNMTAVAVVEAQSEA

Specific function: Required for symbiotic nitrogen fixation. Plays a key role in the conversion of malate to acetyl-CoA for efficient tricarboxylic acid cycle function in nitrogen-fixating bacteria [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=341, Percent_Identity=24.0469208211144, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI62420882, Length=348, Percent_Identity=25.8620689655172, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI62420880, Length=348, Percent_Identity=25.8620689655172, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI239049447, Length=348, Percent_Identity=25.8620689655172, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI4505145, Length=345, Percent_Identity=25.2173913043478, Blast_Score=73, Evalue=1e-12,
Organism=Homo sapiens, GI270265879, Length=317, Percent_Identity=26.4984227129338, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1788806, Length=757, Percent_Identity=58.5204755614267, Blast_Score=908, Evalue=0.0,
Organism=Escherichia coli, GI1788800, Length=318, Percent_Identity=36.1635220125786, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI1788635, Length=325, Percent_Identity=27.0769230769231, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI87081919, Length=316, Percent_Identity=25.3164556962025, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322823, Length=240, Percent_Identity=31.25, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI281362674, Length=399, Percent_Identity=26.0651629072682, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281362672, Length=399, Percent_Identity=26.0651629072682, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21356279, Length=399, Percent_Identity=26.0651629072682, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24646388, Length=345, Percent_Identity=26.9565217391304, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24646386, Length=345, Percent_Identity=26.9565217391304, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.39 [H]

Molecular weight: Translated: 81757; Mature: 81626

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNISEDLKSGALVYHRSPKPGKLEIQATKPLGNQRDLALAYSPGVAAACEAIKADPLQA
CCCHHHHHHCCEEEEECCCCCCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHCCCCCHH
AELTTRANLVAVVSNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIELAATEID
HHHHCCCEEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEHHHHHHH
HLVETVAALEPTFGGINLEDIKAPECFEVEKRLRERMKIPVFHDDQHGTAIIVGAAVRNA
HHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHH
LEIGNRKIEDLKIVTSGAGAAAIACLNLLVTLGAKRENIWVCDIEGVVYEGRETLMDPYK
HHHCCCEECEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEECCEEECCHHHHHHHHH
SVYAQKTDARTLAEVIDGAHIFLGLSAGGVLKPEMVQRMADRPLILALANPNPEIMPEEA
HHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCHH
RAVRPDAMICTGRSDFPNQVNNVLCFPYIFRGALDVGATDINAEMKMAAVEAIAALARET
HHCCCCCEEEECCCCCCHHHHCEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
PSDVVARAYGGETRAFGADSIIPSPFDPRLILRIAPAVAKAAMDTGVATRPIADFDAYNE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHH
KLDEFVFRSGFIMRPLFQRAKQDKKRVIYAEGEDERVLRAAQAVIEEGIAHPILVARPSV
HHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEECCHH
LEARLQRFGLSIRPGKDFEVINPEDDPRYRDFVRSYIEIAGRRGVTPDAARTLVRTSSTV
HHHHHHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
ISALAVKKGEADAMLCGIEGRFSRHLRHVRDIIGLAPGVRELAALSLLITPKGNLFLCDT
HHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHEEEEEECCCCEEEEEC
QVQTEPNAADLAEMTILAAAHVRRFGIEPKVALLSHSNFGSNDTVCARRVRAALDILKDR
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCC
APELEVDGEMQAELALLPDARERILPHSRLQGVANVLVMPDLDAADIAYNMIKVLGDALP
CCCCEECCCCCEEEEECCCHHHHCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHCC
VGPILMGTAKPAHILGPTVTARGIVNMTAVAVVEAQSEA
CCCEEECCCCCCHHCCCCHHHHHHHHEEEEEEEECCCCC
>Mature Secondary Structure 
SNISEDLKSGALVYHRSPKPGKLEIQATKPLGNQRDLALAYSPGVAAACEAIKADPLQA
CCHHHHHHCCEEEEECCCCCCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHCCCCCHH
AELTTRANLVAVVSNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIELAATEID
HHHHCCCEEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEHHHHHHH
HLVETVAALEPTFGGINLEDIKAPECFEVEKRLRERMKIPVFHDDQHGTAIIVGAAVRNA
HHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHH
LEIGNRKIEDLKIVTSGAGAAAIACLNLLVTLGAKRENIWVCDIEGVVYEGRETLMDPYK
HHHCCCEECEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEECCEEECCHHHHHHHHH
SVYAQKTDARTLAEVIDGAHIFLGLSAGGVLKPEMVQRMADRPLILALANPNPEIMPEEA
HHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCHH
RAVRPDAMICTGRSDFPNQVNNVLCFPYIFRGALDVGATDINAEMKMAAVEAIAALARET
HHCCCCCEEEECCCCCCHHHHCEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
PSDVVARAYGGETRAFGADSIIPSPFDPRLILRIAPAVAKAAMDTGVATRPIADFDAYNE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHH
KLDEFVFRSGFIMRPLFQRAKQDKKRVIYAEGEDERVLRAAQAVIEEGIAHPILVARPSV
HHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEECCHH
LEARLQRFGLSIRPGKDFEVINPEDDPRYRDFVRSYIEIAGRRGVTPDAARTLVRTSSTV
HHHHHHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
ISALAVKKGEADAMLCGIEGRFSRHLRHVRDIIGLAPGVRELAALSLLITPKGNLFLCDT
HHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHEEEEEECCCCEEEEEC
QVQTEPNAADLAEMTILAAAHVRRFGIEPKVALLSHSNFGSNDTVCARRVRAALDILKDR
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCC
APELEVDGEMQAELALLPDARERILPHSRLQGVANVLVMPDLDAADIAYNMIKVLGDALP
CCCCEECCCCCEEEEECCCHHHHCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHCC
VGPILMGTAKPAHILGPTVTARGIVNMTAVAVVEAQSEA
CCCEEECCCCCCHHCCCCHHHHHHHHEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535928; 11481430; 10407149 [H]