Click here to switch to the map view.

The map label for this gene is tyrS [H]

Identifier: 158425243

GI number: 158425243

Start: 4148929

End: 4150242

Strand: Direct

Name: tyrS [H]

Synonym: AZC_3619

Alternate gene names: 158425243

Gene position: 4148929-4150242 (Clockwise)

Preceding gene: 158425241

Following gene: 158425246

Centisome position: 77.26

GC content: 65.53

Gene sequence:

>1314_bases
ATGACCACCCTTCGCTCCGATTTCCTGCGCACGCTCCAAGAGCGCGGCTACATCCACCAGTGCTCGGATCTCGAGCGCCT
CGACGCCAAGGCGAGCGAAGGGCCGATCACCGCTTATATCGGCTTCGATGCCACAGCCTCCTCGCTGCATGCCGGGCATC
TGCTCTCCATCATGATGCTGCGCACGCTCCAGCGCACCGGCCACCGGCCGATCGCTCTCATGGGCGGCGGCACCACGAAG
ATCGGCGACCCGTCCGGCAAGGACGAGGCACGCAAGATGCTCACCGATGCGCAGATCAACGAGAACATCGCCTCCATCCG
CAAGGTGTTCGCCCGCTTCCTCGATTTCGAGGGTTCCCCAAAAGGCTACGCGAGATTTGCGCGGGCTGTAGACGGCAAGT
GGAATGAAGAGCCCCGCCTCCTTCCGGCCATCATGGAGAACAATGCGAACTGGCTGGACGAGCTGAAGTACATTCCCCTG
CTGCGCGAGGTGGGGCCGCACTTCACCATCAACCGGATGCTGACGTTCGATTCGGTGAAGCTGCGGCTGGAGCGCGAACA
GCCCCTCACCTTCCTCGAATTCAACTACATGATCCTGCAGGCCTATGACTTCGTGGAGCTGAACAAGCGCCACGGCTGCA
CCCTGCAGATGGGCGGCTCCGACCAGTGGGGCAACATCGTCAACGGCATGGAACTGGGCCGGCGGATGCACGGGGCCGAT
CTGTTCGCCCTCACCACCCCGCTGCTCACCACCTCCTCCGGCGCCAAGATGGGCAAGACGGCGGGCGGTGCGGTGTGGCT
CGATGCGGCGCTGCTGTCGCCTTACGAATACTGGCAGTACTGGCGCAATACCGGCGATGCGGACGTGGAGCGCTTCCTGA
AGCTCTTCACCGAGCTGCCGCTGGACGAGATCGCCCGCCTTGCCGCGCTGGGTGGCCAGGAGATCAACCACGCCAAGGAC
GTGCTCGCCACCGAGGCGACCGCCCTGCTGCATGGCCGCGCCGCTGCGGAGGAGGCCAAGGCCACGGCCCGCGCCACCTT
CGAGGAGGGCGCGCTCTCCACCAACCTGCCCACCATCGAGATCCCGGCGGCCGATCTGAAGGCGGGTCTTGCCGCCAACA
GCGCCTTCGTGACCGCCGGTCTCGTGCCCTCGACCAGCGAGGCGCGCCGCCAGATCAAGTCCGGCGGCCTCAAGGTGAAT
GACGCCACCGTCACCGACGAGAAGCTGGTGCTGGGCGAGCGCGACCTCACCGCCGAGGGCGTGATCAAGCTCTCCATGGG
CAAGAAGAAGCACGTCCTCGTCCGCCCCGTCTGA

Upstream 100 bases:

>100_bases
CGCATTGTCCCCCGCATGGCACCCTTCCCGCTCCGCCCGGTGCATTCCCGGCGAAGACCTGTTACATCACCGCTCCTTTC
CGTCATCCGAACAGATCGAC

Downstream 100 bases:

>100_bases
ACGGACGGACGACGTTGAAAATAGGAACGGCGGGCCATCGGCCCGCCGTTCGCGTTTCAGGAGCAGGACCGGTCGCCCCG
CTCACTTTGCGGGCGTGAGC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MTTLRSDFLRTLQERGYIHQCSDLERLDAKASEGPITAYIGFDATASSLHAGHLLSIMMLRTLQRTGHRPIALMGGGTTK
IGDPSGKDEARKMLTDAQINENIASIRKVFARFLDFEGSPKGYARFARAVDGKWNEEPRLLPAIMENNANWLDELKYIPL
LREVGPHFTINRMLTFDSVKLRLEREQPLTFLEFNYMILQAYDFVELNKRHGCTLQMGGSDQWGNIVNGMELGRRMHGAD
LFALTTPLLTTSSGAKMGKTAGGAVWLDAALLSPYEYWQYWRNTGDADVERFLKLFTELPLDEIARLAALGGQEINHAKD
VLATEATALLHGRAAAEEAKATARATFEEGALSTNLPTIEIPAADLKAGLAANSAFVTAGLVPSTSEARRQIKSGGLKVN
DATVTDEKLVLGERDLTAEGVIKLSMGKKKHVLVRPV

Sequences:

>Translated_437_residues
MTTLRSDFLRTLQERGYIHQCSDLERLDAKASEGPITAYIGFDATASSLHAGHLLSIMMLRTLQRTGHRPIALMGGGTTK
IGDPSGKDEARKMLTDAQINENIASIRKVFARFLDFEGSPKGYARFARAVDGKWNEEPRLLPAIMENNANWLDELKYIPL
LREVGPHFTINRMLTFDSVKLRLEREQPLTFLEFNYMILQAYDFVELNKRHGCTLQMGGSDQWGNIVNGMELGRRMHGAD
LFALTTPLLTTSSGAKMGKTAGGAVWLDAALLSPYEYWQYWRNTGDADVERFLKLFTELPLDEIARLAALGGQEINHAKD
VLATEATALLHGRAAAEEAKATARATFEEGALSTNLPTIEIPAADLKAGLAANSAFVTAGLVPSTSEARRQIKSGGLKVN
DATVTDEKLVLGERDLTAEGVIKLSMGKKKHVLVRPV
>Mature_436_residues
TTLRSDFLRTLQERGYIHQCSDLERLDAKASEGPITAYIGFDATASSLHAGHLLSIMMLRTLQRTGHRPIALMGGGTTKI
GDPSGKDEARKMLTDAQINENIASIRKVFARFLDFEGSPKGYARFARAVDGKWNEEPRLLPAIMENNANWLDELKYIPLL
REVGPHFTINRMLTFDSVKLRLEREQPLTFLEFNYMILQAYDFVELNKRHGCTLQMGGSDQWGNIVNGMELGRRMHGADL
FALTTPLLTTSSGAKMGKTAGGAVWLDAALLSPYEYWQYWRNTGDADVERFLKLFTELPLDEIARLAALGGQEINHAKDV
LATEATALLHGRAAAEEAKATARATFEEGALSTNLPTIEIPAADLKAGLAANSAFVTAGLVPSTSEARRQIKSGGLKVND
ATVTDEKLVLGERDLTAEGVIKLSMGKKKHVLVRPV

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=425, Percent_Identity=34.8235294117647, Blast_Score=231, Evalue=1e-60,
Organism=Escherichia coli, GI1787925, Length=442, Percent_Identity=39.8190045248869, Blast_Score=324, Evalue=7e-90,
Organism=Caenorhabditis elegans, GI25145802, Length=349, Percent_Identity=32.9512893982808, Blast_Score=167, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI25145805, Length=210, Percent_Identity=34.2857142857143, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6325160, Length=455, Percent_Identity=34.2857142857143, Blast_Score=223, Evalue=6e-59,
Organism=Drosophila melanogaster, GI20130383, Length=445, Percent_Identity=37.3033707865169, Blast_Score=256, Evalue=3e-68,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 48072; Mature: 47940

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLRSDFLRTLQERGYIHQCSDLERLDAKASEGPITAYIGFDATASSLHAGHLLSIMML
CCCHHHHHHHHHHHCCCEECCCCHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
RTLQRTGHRPIALMGGGTTKIGDPSGKDEARKMLTDAQINENIASIRKVFARFLDFEGSP
HHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KGYARFARAVDGKWNEEPRLLPAIMENNANWLDELKYIPLLREVGPHFTINRMLTFDSVK
HHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCEE
LRLEREQPLTFLEFNYMILQAYDFVELNKRHGCTLQMGGSDQWGNIVNGMELGRRMHGAD
EEEECCCCCEEEECCEEEEEEHHHHEECCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCC
LFALTTPLLTTSSGAKMGKTAGGAVWLDAALLSPYEYWQYWRNTGDADVERFLKLFTELP
EEEECCCCEECCCCCCCCCCCCCHHEEHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHCC
LDEIARLAALGGQEINHAKDVLATEATALLHGRAAAEEAKATARATFEEGALSTNLPTIE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
IPAADLKAGLAANSAFVTAGLVPSTSEARRQIKSGGLKVNDATVTDEKLVLGERDLTAEG
ECHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCEEEECCCCCCCEEEECCCCCCCCC
VIKLSMGKKKHVLVRPV
EEEEECCCCCCEEEECC
>Mature Secondary Structure 
TTLRSDFLRTLQERGYIHQCSDLERLDAKASEGPITAYIGFDATASSLHAGHLLSIMML
CCHHHHHHHHHHHCCCEECCCCHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
RTLQRTGHRPIALMGGGTTKIGDPSGKDEARKMLTDAQINENIASIRKVFARFLDFEGSP
HHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KGYARFARAVDGKWNEEPRLLPAIMENNANWLDELKYIPLLREVGPHFTINRMLTFDSVK
HHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCEE
LRLEREQPLTFLEFNYMILQAYDFVELNKRHGCTLQMGGSDQWGNIVNGMELGRRMHGAD
EEEECCCCCEEEECCEEEEEEHHHHEECCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCC
LFALTTPLLTTSSGAKMGKTAGGAVWLDAALLSPYEYWQYWRNTGDADVERFLKLFTELP
EEEECCCCEECCCCCCCCCCCCCHHEEHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHCC
LDEIARLAALGGQEINHAKDVLATEATALLHGRAAAEEAKATARATFEEGALSTNLPTIE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
IPAADLKAGLAANSAFVTAGLVPSTSEARRQIKSGGLKVNDATVTDEKLVLGERDLTAEG
ECHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCEEEECCCCCCCEEEECCCCCCCCC
VIKLSMGKKKHVLVRPV
EEEEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA